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Acta Pharmacologica Sinica 2005年第2期26卷 171-176页

作者： Sun ZHANG~2,Ying HUANG~2,Yi-chun ZHU~2,Tai YAO~(2,3,4)~2Department of Physiology and Pathophysiology ~3state key laboratory for medical neurobiology,shanghai medical college of fudanuniversity,shanghai 200032,china Department of Physiology and Pathophysiology shanghai medical college of fudanuniversity Shanghai 200032 China

Aim:To investigate the mechanism of the action of estrogen,which stimulates therelease of secreted amyloid precursor protein α(sAPPα)and decreases the gen-eration of amyloid-β protein(Aβ),a dominant component in senile plaques in thebrains of Alzheimer's disease ***:Experiments were carried out inprimary rat cortical neurons,and Western blot was used to detect sAPPα in aculture medium and the total amount of cellular amyloid precursor protein(APP)***:17β-Estradiol(but not 17α-estradiol)and β-estradiol 6-(O-carboxymethyl)oxime:BSA increased the secretion of sAPPα and this effect wasblocked by protein kinase C(PKC)inhibitor calphostin C,but not by the classicalestrogen receptor antagonist ICI 182,***,17β-estradiol did not alterthe synthesis of cellular ***:The effect of 17β-estradiol on sAPPαsecretion is likely mediated through the membrane binding sites,and needs mo-lecular configuration specificity of the ***,the action of the PKC-dependent pathway might be involved in estrogen-induced sAPPα secretion.

关键词： Alzheimer disease estrogen amyloid precursor protein estrogen receptors protein kinase C

All-trans retinoic acid inhibited angiotensin Ⅱ-induced increase in cell growth and collagen secretion of neonatal cardiac fibroblasts

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Acta Pharmacologica Sinica 2006年第4期27卷 423-429页

作者： Yan HE~2 Ying HUANG~2 Li ZHOU~2 Li-min LU~2 Yi-chun ZHU~2 Tai YAO~(2,3,4) ~2Department of Physiology and Pathophysiology,and ~3state key laboratory for medical neurobiology.Fudan University shanghai medical college.shanghai 200032.china Department of Physiology and Pathophysiology Fudan University Shanghai Medical College Shanghai China state key laboratory for medical neurobiology Fudan University Shanghai Medical College Shanghai China

Aim:To determine whether all-trans retinoic acid(atRA)acts to modulate angio-tensin Ⅱ(Ang Ⅱ)-induced cardiac fibroblast cell growth and collagen ***:Cultured neonatal rat cardiac fibroblasts(CF)were used in the experiment.A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)assay wasused to detect cell growth of the CF;and immunocytochemistry and Westernblotting were used to measure the production and secretion of collagen and theexpression of transforming growth factor-β1(TGF-β1)by the ***:atRA(1×10-7to 1×10-5mol/L)inhibited the Ang Ⅱ-induced increase in cell growth of CF(P3319 (up to 1×10-6mol/L).Exposure of CF toatRA(1×10-5mol/L)attenuated the Ang Ⅱ-induced increase in the secretion ofcollagen types Ⅰ and Ⅲ(P<0.05).atRA (1×10-5mol/L)also blocked the Ang Ⅱ-induced increase in the expression of TGF-β***:atRA inhibits the AngⅡ-induced increase in cell growth and collagen secretion of neonatal rat *** of atRA is possibly mediated by lowering the TGF-β*** observa-tions support the notion that atRA is a potential candidate for the prevention andtherapy of cardiac remodeling.

关键词： cardiac fibroblast angiotensin Ⅱ all-trans retinoic acid collagen secretion transforming growth factor-β1

The Mystery of Sleep:Molecular Mechanisms of Histaminergic Roles in Sleep-wake Regulation

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生物物理学报 2009年第S1期25卷 52-53页

作者： Zhi-Li Huang1,2,Wei-Min Qu2 1 state key laboratory of medical neurobiology 2 Department of Pharmacology, shanghai medical college of Fudan University, shanghai 200032, china.

The histaminergic neurons are confined to the tuberomammillary nucleus (TMN) of the posterior hypothalamus, and send their fibers to almost all regions of the brain. A

关键词： TMN

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Differential sensitivity of GABAergic and glycinergic inputs to orexin-A in preganglionic cardiac vagal neurons of newborn rats

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Acta Pharmacologica Sinica 2005年第12期26卷 1442-1447页

作者： Ji-jiang WANG~2 Yong-hua CHEN Ke-yong LI Feng-yan SUN National key laboratory of medical neurobiology,Fudan University shanghai medical college,shanghai 200032,china Nationalkeylaboratoryofmedicalneurobiology FudanUniversityShanghaiMedicalCollegeShanghai200032China

Aim:To test the effect of orexin-A(hypocretin-1),a neuropeptide synthesized inthe lateral hypothalamus and the perifornical area,on the glycinergic inputs andthe GABAergic inputs of cardiac vagal neurons(CVN).Methods:The effects oforexin-A at three concentrations(20 nmol/L,100 nmol/L,500 nmol/L)on theglycinergic inputs and the GABAergic inputs were investigated by using retro-grade fluorescent labeling of cardiac neurons(CVN)in the nucleus ambiguus(NA)and the voltage patch-clamp ***:Orexin-A dose-depen-dently increased the frequency of both the glycinergic and the GABAergic spon-taneous inhibitory postsynaptic currents(sIPSC).However,at a lower concentra-tion(20 nmol/L)of orexin-A,although the frequency of the glycinergic sIPSC wassignificantly increased,the frequency of the GABAergic sIPSC was not signifi-cantly ***:The glycinergic inputs and the GABAergic inputshave different sensitivities to orexin-A,which suggests that the two kinds ofinhibitory inputs might play different roles in the synaptic control of cardiac vagalfunctions.

关键词： patch-clamp techniques nervous system synaptic transmission vagus nerve

Altered neuromodulation sensitivity of two epilepsy related SCNIA mutations by protein kinase A

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Altered neuromodulation sensitivity of two epilepsy related ...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Shuai LIU,Ping ZHENG~* state key laboratory of medical neurobiology,Fudan University shanghai medical college,shanghai 200032, china

＜正＞Over the past 10 years,mutations in voltage-gated sodium channel subtype one（Gene symbol SCNl A） have become closely associated with inheritable forms of *** the chief culprit,hundreds of SCNl A mutations that cause epilepsy have been identified *** of the former studies reported that biophysical characteristics of these mutations may change to a certain extent varied in different cases,while no elucidate conclusion has been justified. In present experiment,we aim to investigate if there is an underlying alternation in neuromodulation sensitivity beyond the change of biophysical ***-type（WT） SCN1A cDNA and two epilepsy related mutations were transfected into HEK293t cells *** cells were treated by forskolin in different concentrations to activate PKA *** properties of WT channels and mutant channels were recorded before and after the treatment of forskolin via whole-cell patch-clamp *** results show that forskolin inhibited peak current amplitude of both WT and mutant channels in a dose-dependent manner,however peak current amplitude of mutant channels decrease less than that of WT channels and the difference enlarged as the dose of forskolin increased（statistically different in 3μM and 10μM）.Steady-state inactivation curve of WT showed a remarkable negative shift after forskolin application while both mutant channels showed relatively slight *** the part of recovery from fast inactivation,mutant channels also showed insensitivity to the delay effect of forskolin compared to WT *** results above are mostly in accordance with the results we recently obtained from transfected primary neuronal *** is suggested that these epilepsy-related mutant channels possess altered neuromodulation sensitivity which can be probably enlarged in signal transduction, impairing the delicate balances between chemical and electrical harmony in the central nervous system,and fina

关键词： epilepsy voltage-gated sodium channel mutation PKA patch clamp

Induction of CRMP-4 in striatum of adult rat after transient brain ischemia

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Acta Pharmacologica Sinica 2003年第12期24卷 31-37页

作者： LIU Peng-Chong YANG Zeng-Jin QIU Mei-Hong, ZHANG Ling-Mei, SUN Feng-Yan National laboratory of medical neurobiology, Fudan University shanghai medical college, shanghai 200032, china National laboratory of medical neurobiology Fudan University Shanghai Medical College Shanghai 200032 Chin

AIM: To study the expression of collapsing response mediated protein-4 (CRMP-4) and nestin in the ischemic adult rat brain following transient brain ischemia. METHODS: Brain ischemia was induced by transient left middle cerebral artery occlusion (MCAO) for 60 min in adult rats. The expression of CRMP-4, nestin and bromodeoxyuridine (BrdU) was analyzed by immunohistochemical method. The co-localization of CRMP-4 and nestin or BrdU was analyzed by double staining combined with confocal laser scanning microscopy. RESULTS: CRMP-4, a marker of immature neuron, could be expressed in the ipsilateral striatum and cerebral cortex at 1st and 2nd week after the ischemia-reperfusion; nestin, a marker of neural stem cell, occurred in above regions from several hours to 2 weeks. CRMP-4 costained with nestin and with BrdU incorporation. CONCLUSION: Neural stem cells may present in the striatum and cerebral cortex of adult rat and can be triggered to differentiate into newborn neuron there by ischemic brain trauma.

关键词： brain ischemia corpus striatum CRMP-4 protein intermediate filament proteins bromodeoxyuridine neurons regeneration

Sigma-1 receptors amplify dopamine D1 receptor signaling at presynaptic sites in the prelimbic cortex

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Sigma-1 receptors amplify dopamine D1 receptor signaling at ...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Wen-Jie LUAN,Ping ZHENG state key laboratory of medical neurobiology,shanghai medical college,Fudan University,shanghai 200032, china

Sigma-1 receptors are highly expressed in the *** downstream signaling mechanisms associated with the sigma-1 receptor activation have been shown to involve the activation of protein kinase C(PKC),the control of Cahomoeostasis and the regulation of voltage-and ligand-gated ion *** few studies examined the regulatory effect of sigma-1 receptors on metabotropic receptor *** present paper studied the regulatory effect of sigma-1 receptors on the signaling of dopamine D receptors,one of metabotropic receptors,by examining the effect of sigma-1 receptor agonists on the D receptor agonist-induced cAMP-dependent protein kinase(PKA) activation at presynaptic sites using the synaptosomes from the prelimbic *** results showed that sigma-1 receptor agonists alone had no effects on the PKA activity,but could amplify the D receptor agonist-induced PKA *** sigma-1 receptor agonist also amplified the membrane-permeable analog of cAMP-and the adenylyl cyclase(AC)activator-induced PKA activation,but did not on the D receptor agonist-induced AC *** conventional PKC(cPKC),especially the PKCβI,and the extracellular Cainflux through L-type Cachannels might play key roles in the amplifying effect of the sigma-1 receptor *** activation of PKC by sigma-1 receptor agonists was the upstream event of the increase in the intrasynaptosomal *** results suggest that sigma-1 receptors may amplify the D receptor agonist-induced PKA activation by sigma-1 receptors-cPKC(especially the PKCβI)-L-type Cachannels-Ca-AC and/or cAMP signaling pathway.

关键词： sigma-1 receptors synaptosomes prelimbic cortex D1 receptors cAMP-dependent protein kinase protein kinase C

Pi-linked D1 receptors modulate classical dopamine D1 receptor/cAMP/PKA pathway at presynaptic sites in the prelimbic cortex

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Pi-linked D1 receptors modulate classical dopamine D1 recept...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Wen-Jie LUAN,Ping ZHENG state key laboratory of medical neurobiology,shanghai medical college,Fudan University,shanghai 200032, china

Inositol 1,4,5-trisphosphate(InsP)and cAMP are the two second messengers that play an important role in neuronal ***,we investigated the interactions of InsP-and cAMP-mediated signaling pathways activated by dopamine at presynaptic sites in the prelimbic *** present paper studied the regulatory effect of phosphatidylinositol(PI)-linked D-like receptor on the signaling of classical dopamine D receptors by examining the effect of Pi-linked D-like receptor agonists SKF83959 on the D,receptor agonist-induced cAMP-dependent protein kinase(PKA)activation at presynaptic sites using the synaptosomes from the prelimbic *** results showed that SKF83959 alone had no effects on the PKA activity,but could amplify the classical D receptor agonist-induced PKA *** conventional PKC(cPKC),especially the PKCa,and the extracellular Cainflux,Phospho-DARPP -32 Thr75 also play key roles in the modulate effect of the *** results suggest that Pi-linked D-like receptors may modulate the classical D,receptor agonist-induced PKA activation by Pi-linked D-like receptors-cPKC (especially the PKCa)-Ca-Cdk5-Phospho-DAPRR-32 Thr 75 signaling *** may provide a potential target for the drug discovery in Parkinson’s disease.

关键词： Pi-linked D1-like receptors prelimbic cortex classical D1 receptors cAMP-dependent protein kinase protein kinase C DARPP-32

Altered responses of sodium channels to cAMP-dependent protein kinase activator in an epilepsy-associated SCNIA mutation

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Altered responses of sodium channels to cAMP-dependent prote...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Shuai LIU,Ping ZHENG state key laboratory of medical neurobiology,shanghai medical college and Institutes of Brain Science,Fudan University,shanghai 200032,china

＜正＞Familial inherited epilepsy with febrile seizures plus（GEFS+）caused by the mutation（I1656M）in SCN1A,the gene encoding the brain voltage-gated sodium channel a,subunit,has received a considerable attention because it provides a novel epileptic model to understand the pathogenesis of *** studies examined biophysical characteristics of I1656M using whole-cell patch-clamp analysis of heterologously expressed recombinant human *** the present paper,we examined changes of channel properties in responses to cAMP-dependent protein kinase（PKA）activator forskolin and protein kinase C（PKC）activator PDBu in the mutant channels by using whole-cell patch-clamp *** results showed that the response of the amplitude of sodium currents to forskolin significantly decreased in the mutant channels,but did not to *** addition,the response of the voltage dependence of activation and fast inactivation to forskolin disappeared in mutant channels,but the response of the voltage dependence of slow inactivation did *** overall effect of PKA activation on neuronal excitability were opposite in the mutant *** results indicate that altered responses of the mutant channels to PKA signaling may impair the delicate balances between chemical and electrical harmony and lead to abnormal neuronal excitability.

关键词： epilepsy familial inherited epilepsy with febrile seizures plus Nav1.1 SCN1A mutation whole-cell patchclamp cAMP-dependent protein kinase

Inhibition of Qi and Qo sites of mitochondrial complexⅢinduced opposite effects on HVA calcium channels via activation of different protein kinase C isoforms in cortical neurons

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Inhibition of Qi and Qo sites of mitochondrial complexⅢindu...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Pei-Ying WU,Bin LAI,Ping ZHENG~* state key laboratory of medical neurobiology,Fudan University,shanghai medical college,shanghai 200032, china

＜正＞Objective Inhibition of Qi and Qo sites of mitochondrial complexⅢunder hypoxia and ischemia has received attention,but its downstream targets remain largely *** work showed altered function of sodium channels after the inhibition of complexⅢ.HVA calcium channels were well recognized to be an important factor in ischemia-induced cell *** explore whether the inhibition of mitochondrial complexⅢhas any effect on the function of HVA calcium channels and the related downstream pathways will shed light on our understanding about the complicated events after hypoxia/*** Electrophysiological recording of HVA calcium currents was carried out on slice preparations from 14-day-old rats using whole cell recording *** of Qi and Qo sites of mitochondrial complexⅢwas mimicked by application of specific inhibitors antimycin A and myxothiazol *** drugs were applied through the bath ***（1） Antimycin A decreased the peak amplitude of HVA calcium current while myxothiazol increased it.（2） Antimycin A induced the effect via the activation ofβ1 isoform of protein kinase C while myxothiazol increased the amplitude of calcium currents through the activation ofδisoform.（3） The effect of antimycin A on HVA calcium channels could be abolished by the application of antioxidants PHEN and MPG while the superoxide scavenger MnTB Aβand SOD could cancel the effect of *** Inhibition of mitochondrial complex HI at different sites exerted opposite effects on HVA calcium channels.

关键词： cortical neuron whole cell patch-clamp mitochondria complexⅢ Qi site Qo site antimycin A myxothiazol HVA calcium channels Protein Kinase C isoforms reactive oxygen species superoxide