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Effects of depside salt from salvia miltiorrhiza on platelet endothelial nitric oxide synthase activity

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上海医学 2007年第S1期30卷 182-183页

作者： WANG Wen XU Biao department of Cardiology, Affiliated drum tower hospital of nanjing University Medical School, nanjing 210008, china

Objectivs To observe the changes of platelet endothelial nitric oxide synthase (eNOS) activity and effects of depside salt from salvia miltiorrhiza on platelet eNOS activity in health adult. Methods Peripheral venous

关键词： salvia Platelet dosage stimulated arginine aggregation

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Effect of adjunctive tirofiban therapy on myocardial perfusion in patients with ST-segment elevated myocardial infarction undergoing primary percutaneous coronary intervention

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上海医学 2007年第S1期30卷 117-118页

作者： WANG Lian XU Biao ZHANG Rong-lin department of Cardiology, nanjing drum tower hospital Affiliated to Medical School of nanjing University, nanjing 210008,china

Objective To investigate the effect of glycoprotein II b/IIIa receptor blockade with tirofiban on myocardial perfusion in patients with ST-segment elevated myocardial infarction ( STEMI ) undergoing

关键词： perfusion elevated glycoprotein elevation bleeding occlusion minutes restored markedly slightly

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Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity

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Acta Pharmacologica Sinica 2006年第5期27卷 588-596页

作者： Yun LIU~(2,3) Wen-lan SUN~3 Yan SUN~3 Gang HU~(2,4) Guo-xian DING~(3,4) ~2department of Pharmaeology,nanjing Medical University,nanjing 210029,china ~3department of Geratology,the First hospital Affiliated to Nan jinx,Medical University,nanjing 210008,china department of Pharmacology Nanjing Medical University Nanjing China department of Geratology the First Hospital Affiliated to Nanjing Medical University Nanjing China

Aim:To observe the roles of 11-beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in in vitro preadipocyte differentiation and in rats with diet-induced obe-sity (DIO).Methods:Protein expression of 11β-HSD1 in the process of 3T3-L1 celldifferentiation and in various tissues of the rats were detected by Western blotanalysis;expression of 11β-HSD1 mRNA and glucocorticoid receptor (GR) andother marker genes of preadipocyte differentiation were detected by using real-time ***:Lipid droplets in 3T3-L1 cells accumulated and increased afterstimulation.A dramatically elevated protein level of 11β-HSD1,especially in thelate stages of 3T3-L1 cell differentiation,was *** relative mRNA levelsof 11β-HSD1,GR and cell differentiation markers LPL,aP2,and FAS wereupregulated,and Pref-1 was downregulated during the *** DIO rats,bodyweight,visceral adipose mass index and the protein expression of 11β-HSD1increased,especially in adipose tissue,brain and *** insulin,triglyceride,total cholesterol and low-density lipoprotein cholesterol were foundto be increased in DIO rats,but without any obvious changes in blood glucose ortumor necrosis factor-α ***:11β-HSDI may promote preadipocytedifferentiation,and may be involved in the development of obesity.

关键词： 11-beta-hydroxysteroid dehydrogenase type 1 glucocorticoids adipocytes cell differentiation obesity

11 beta-hydroxysteroid dehydrogenase type 1 promotes differentiation of 3T3-L1 preadipocyte

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Acta Pharmacologica Sinica 2007年第8期28卷 1198-1204页

作者： Yun LIU~(2,3,4) Yan SUN~(2,4) Ting ZHU~2 Yu XIE~2 Jing YU~2 Wen-lan SUN~2 Guo-xian DING~(2,5) Gang HU~(3,5) ~2department of Geratology the First Affiliated hospital,nanjing Medical University,nanjing 210008,china ~3department of Pharmacology,nanjing Medical University,nanjing 210029,china department of Geratology the First Affiliated Hospital Nanjing Medical University Nanjing China department of Pharmacology Nanjing Medical University Nanjing China

Aim:To investigate the relationship between 11 beta-hydroxysteroid dehydroge-nase type 1(1 lbeta-HSD1),a potential link between obesity and type 2 diabetes,and preadipocyte ***:Mouse 1 lbeta-HSD 1 siRNA plasmidswere transfected into 3T3-L1 preadipocytes(a cell line derived from mouseSwiss3T3 cells that were isolated from mouse embryo),for examination of theeffect of targeted 11 beta-HSD 1 inhibition on differentiation of 3T3-L 1 ***-ferentiation was stimulated with 3-isobutyl-l-methyxanthine,insulin,*** transcription level of the genes was detected by ***:Lipid accumulation was significantly inhibited in cells transfectedwith mouse 1 lbeta-HSD1 siRNA compared with non-transfected 3T3-L1 *** lipid droplets were detected in the transfected cells both prior to stimulationand after stimulation with differentiation-inducing *** expression ofadipocyte differentiation-associated markers such as lipoprotein lipase and fattyacid synthetase were downregulated in the transfected ***,the expres-sion of preadipocyte factor-1,an inhibitor of adipocyte differentiation,wasdownregulated upon stimulation of differentiation and had no changes in thetransfected ***:11 beta-HSD1 can promote *** on this,we propose that 11 beta-HSD1 may be an importantcandidate mediator of obesity and obesity-induced insulin resistance.

关键词： 11 beta-hydroxysteroid dehydrogenase type 1 siRNA 3T3-L1 cell differentiation obesity

Effects of coffee and caffeine on bladder dysfunction in streptozotocininduced diabetic rats

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Acta Pharmacologica Sinica 2006年第8期27卷 1037-1043页

作者： Chao-ran YI~(2,5) Zhong-qing WEI~2 Xiang-lei DENG~4 Ze-yu SUN~2 Xing-rong LI~4 Cheng-gong TIAN~3 ~2department of Urology,~3department of Endocrinology,the Affiliated drum tower hospital,nanjing University Medical School,nanjing 210008,china ~4department of Biochemisto,and Molecular Biology,Medieal College of Southeast University,nanjing 210009,china department of Urology the Affiliated Drum Tower Hospital Nanjing University Medical School Nanjing China department of Biochemistry and Molecular Biology Medical College of Southeast University Nanjing China department of Endocrinology the Affiliated Drum Tower Hospital Nanjing University Medical School Nanjing China

Aim:To explore the effects and mechanisms of caffeine and coffee on bladderdysfunction in streptozotocin-induced diabetic ***:Sprague-Dawleymale rats were divided randomly into 4 groups:control,diabetes mellitus(DM),DM with coffee treatment,and DM with caffeine *** diabetic rat wasinduced by intraperitoneal injection of streptozotocin(60 mg/kg).After 7 weeks oftreatment with coffee and caffeine,cystometrogram,contractile responses to elec-trical field stimulation(EFS)and acetylcholine(ACh),and cyclic AMP(cAMP)concentration of the bladder body and base were ***:The bladderweight,volume threshold for micturition and post-void residual volume(PVR)inthe diabetic rats were significantly higher compared to those in the control *** or caffeine treatment significantly reduced the bladder weight,bladdercapacity and PVR in the diabetic *** caused significant decreases in cAMPconcentration of the bladder and coffee and caffeine caused upregulation of cAMPcontent in the diabetic *** addition,coffee and caffeine tended to normal-ize the altered detrusor contractile responses to EFS and ACh in the diabetic ***:These results indicate that caffeine and coffee may have beneficialeffects on bladder dysfunction in the early stage of diabetes by increasing cAMPcontent in the lower urinary tract,recovering the micturition reflex and improvingthe detrusor contractility.

关键词： caffeine diabetic mellitus bladder urodynamics muscle contraction cyclic AMP

Differentiation of embryoid-body cells derived from embryonic stem cells into hepatocytes in alginate microbeads in vitro

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Acta Pharmacologica Sinica 2007年第12期28卷 1924-1930页

作者： Sheng FANG,Yu-dong QIU,Liang MAO,Xiao-lei SHI,De-cai YU,Yi-tao DING~2department of Hepatobiliary Surgery,Affiliated drum tower hospital,Medical College of nanjing University,nanjing 210008,china department of Hepatobiliary Surgery Affiliated Drum Tower Hospital Medical College of Nanjing University Nanjing China

Aim:Embryonic stem (ES) cells are being widely investigated as a promisingsource of hepatocytes with their proliferative,renewable,and ***,controlled and scalable ES cell differentiation culture intofunctional hepatocytes is *** this study,we examined the differentiat-ing potential of embryoid-body cells derived from ES cells into hepatocytes inalginate microbeads containing exogenous growth factors in ***:.Embryoid bodies were formed from ES cells by suspension *** cultured for 5 d were treated with *** disaggregated cellswere encapsulated in alginate microbeads and stimulated with exogenous growthfactors to induce hepatic *** the course of cell differentiation,cellmorphology and viability were observed,and the expression patterns of somegenes of the hepatocyte were confirmed by *** immunofluorescenceanalysis revealed the expression of albumin (ALB) and cytokeratin-18 (CK18).Hepatocyte functional assays were confirmed by the secretion of ALB and ***:We showed that embryoid-body cells could maintain cell viability inalginate microbeads in *** also found that directed differentiated cellsexpressed several hepatocyte genes including α-fetoprotein (AFP),ALB,Cyp7a1,CK18,transthyretin (TTR) and tyrosine aminotransferase (TAT) and producedALB and urea in alginate *** directed differentiated cells expressedALB and CK18 proteins on d ***,embryoid-body cells could not formhepatocytes without exogenous growth factors in alginate ***:The differentiation of embryoid-body cells into hepatocytes con-taining exogenous growth factors in alginate microbeads gives rise to functionalhepatocytes and may develop scalable stem cell differentiation strategies forbioartificial livers and hepatocyte transplantation.

关键词： alginate cell culture encapsulation hepatocytes stem cell differentiation

Caveolin-1 is important for nitric oxide-mediated angiogenesis in fibrin gels with human umbilical vein endothelial cells

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Acta Pharmacologica Sinica 2006年第12期27卷 1567-1574页

作者： Yi-ming PAN Yong-zhong YAO Zhang-hua ZHU Xi-tai SUN Yu-dong QIU Yi-tao DING~2 department of Hepatobiliary Surgery,drum tower hospital,Medical College of nanjing University and Hepatobiliary Institute of nanjing University,nanjing 210008,china department of Hepatobiliary Surgery Drum Tower Hospital Medical College of Nanjing University and Hepatobiliary Institute of Nanjing University Nanjing China

Aim:The role of caveolin-1(Cav-1)in angiogenesis remains poorly *** endothelial nitric oxide(NO)synthase(eNOS),a caveolin-interacting protein,was demonstrated to play a predominant role in vascular endothelial growth fac-tor(VEGF)-induced *** purpose of our study was to examine therole of Cav-1 and the eNOS complex in NO-mediated ***:Human umbilical vein endothelial cells(HUVEC)were isolated and cultured in 3-Dfibrin gels to form capillary-like tubules by VEGF *** expression ofCav-1 and eNOS was detected by semiquantitative *** HUVEC weretreated with antisense oligonucleotides to downregulate Cav-1 *** and non-infected HUVEC were cultured in fibrin gels in the presenceor absence of VEGF(20 ng/mL)and N~G-nitro-L-arginine methyl ester(L-NAME;5 mmol/L).NO was measured using a NO assay kit and capillary-like tubules werequantified by tubule formation index using the Image J ***:RT-PCRanalysis revealed that Cav-1 levels steadily increased in a time-dependent mannerand reached their maximum after 5d of incubation,but there were no obviouschanges in eNOS mRNA expression in response to VEGF in the fibrin gel ***(20 ng/mL)can promote NO production and the formation of capillary-liketubules,and this promoting effect of VEGF was blocked by the addition ofL-NAME(5 mmol/L).When transduced HUVEC with the antisense Cav-1 oligo-nucleotides were plated in the fibrin gels,the capillary-like tubules were signifi-cantly fewer than those of the non-infected *** capillary-like tubules forma-tion and NO production of transduced HUVEC with the antisense Car-1 oligo-nucleotides cultured in fibrin gels showed no responses to the addition of VEGF(20 ng/mL)and L-NAME(5.0 mmol/L).Conclusion:NO was a critical angiogenicmediator in this ***-1 was essential for NO-mediated angiogenesis andmay be an important target of anti-angiogenesis therapy.

关键词： caveolin-1 angiogenesis nitric oxide vascular endothelial growth factor

Hepatocyte-like cells from directed differentiation of mouse bone marrow cells in vitro

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Acta Pharmacologica Sinica 2005年第4期26卷 469-476页

作者： Xiao-lei SHI~2 Yu-dong QIU~2 Qiang LI Ting XIE~2 Zhang-hua ZHU~2 Lei-lei CHEN~3 Lei LI~3 Yi-tao DING~(2,4) 2 department of Hepatobiliary Surgery 3 Scientific Research department,Affiliated drum tower hospital,Medical College of nanjing University,nanjing 210008,china department of Hepatobiliary Surgery Affiliated Drum Tower Hospital Medical College of Nanjing University Nanjing China Scientific Research department Affiliated Drum Tower Hospital Medical College of Nanjing University Nanjing China

Aim:To design the effective directed differentiation medium to differentiate bonemarrow cells into hepatocyte-like ***:Bone marrow cells were cul-tured in the directed differentiation media including fibroblast growth factor-4(FGF-4)and oncostatin M(OSM).Hepatocyte-like cells from directed differentia-tion of bone marrow cells were identified through cell morphology,RNA expres-sions by reverse transcriptase-polymerase chain reaction(RT-PCR),protein ex-pressions by Western blot,and hepatocellular synthesis and metabolism func-tions by albumin ELISA,Periodic acid-Shift staining and urea ***:Some epithelial-like cells or polygonal cells appeared and increased in the courseof the cell directed *** nucleur factor-3[3(HNF-3β),albu-min(ALB),cytokeratin 18(CK18),transthyretin(TTR),glucose-6-phosphate(G-6-Pase),and tyrosine aminotransferase(TAT)mRNA were expressed in the courseof the directed *** directed differentiated cells on d 21 expressedHNF-3β,ALB,and CK18 *** directed differentiated cells producedalbumin and synthesized urea in a time-dependent *** could also syn-thesize ***:Our differentiation media,including FGF-4 andOSM,are effective to differentiate bone marrow cells into hepatocyte-like cells,which could be used for hepatocyte resources for bioartificial liver or hepatocytetransplantation.

关键词： bone marrow cells hepatocytes cell differen tiation stern cells

Mitochondrial uncoupling protein 2 expression in colon cancer and its clinical significance

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World Journal of gastroenterology 2010年第45期16卷 5773-5778页

作者： Xiao-Yi Kuai, Ze-Yu Ji, Hong-Jie Zhang,department of gastroenterology, First Affiliated hospital of nanjing Medical University, nanjing 210029, Jiangsu Province, china department of gastroenterology First Affiliated Hospital of Nanjing Medical University 300 Guangzhou Road Nanjing 210029 Jiangsu Province China.

AIM: To detect the expression of mitochondrial uncoupling protein 2 (UCP2) in colon cancer and analyze the relation between UCP2 expression and clinical pathological features of colon ***: Fifteen colon tissue samples and 15 its adjacent tissue samples were obtained from colon cancer patients during surgical interventions. UCP2 expression was detected with immunohistochemical method in 10 normal controls, 10 hyperplastic polyp patients, 20 tubular adenoma patients and 78 colon cancer patients. Patients with rectal cancer were excluded. Quantitative reverse transcription polymerase chain reaction and Western blotting were used to detect UCP2 expressions in colon cancer tissue samples and its adjacent tissue samples. Relation between UCP2 expression and clinical pathological features of colon cancer was also analyzed. RESULTS: The UCP2 mRNA expression level was fourfold higher in colon cancer tissue samples than in its adjacent tissue samples. The UCP2 protein expression level was three-fold higher in colon cancer tissue samples than in its adjacent normal tissue samples. The UCP2 was mainly expressed in cytoplasm. The UCP2 was not expressed in normal colon mucosa. Strong positive staining for UCP2 with a diffuse distribution pattern was identified throughout the mucosa in colon cancer tissue samples with a positive expression rate of 85.9%. The UCP2 expression level was higher in colon cancer tissue samples at clinical stages Ⅲ and Ⅳ than in those at stageⅠ+ Ⅱ. Univariate analysis showed that the high UCP2 expression level was significantly correlated to colon cancer metastasis (hazard ratio = 4.321, confidence interval = 0.035-0.682, P = 0.046). CONCLUSION: UCP2 is highly expressed in human colon cancer tissue and may be involved in colon cancer metastasis.

关键词： Mitochondrial uncoupling protein 2 Colon cancer Uncoupling protein 2 Clinicopathologic characteristics