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Inhibition of caspases and intracellular free Ca2+ concentrations are involved in resveratrol protection against apoptosis in rat primary neuron cultures

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Acta Pharmacologica Sinica 2007年第11期28卷 1724-1730页

作者： Qi-hai GONG~(2,3.4),Qian WANG~(2,3.,Jing-shan SHI~(2,3.,Xie-nan HUANG~(2,3.,Qiong LIU~2,Hu MA~2~2 Department of pharmacology,zunyi medical college,{3. 563.00,China ~3.the Key Laboratory of Basic pharmacology of GuizhouProvince,zunyi medical college,{3. 563.00,China ment of pharmacology Zunyi Medical College Zunyi 563000 China. gongqihai@***.

Aim:To investigate the influence of resveratrol （Res）,a nutritional antioxidant,onthe inhibition of apoptosis in rat primary neuron ***:The culturedcortical neurons of neonatal Sprague-Dawley rats were pretreated with Res （0.1,1.0,and 10.0 μmol/L） and oxygen-glucose deprivation/reperfusion （OGD/RP） withoxygen and glucose were initiated at d 10 in *** apoptosis wasdetermined by flow cytometry,and morphological changes of neurons wereobserved by an electron *** the mechanism studies,the intracellularfree calcium concentration ([Ca2+]i) and the transcription of caspases-3.and-12 inneurons were detected by Fura 2/AM loading and real-time RT-PCR,***:OGD/RP insult could induce an increase in the apoptotic rate of neurons（from 11.1% to 49.0%）,and elicit an obvious morphological change in neurons;pretreatments with Res （0.1,1.0,and 10.0 μmol/L,respectively） significantlyreduced the elevated rate of apoptosis to 41.7%,40.8%,and 3..4%,respectively,and ameliorated the neuronal morphological ***,the OGD/RP insultobviously elicited the elevated levels of the [Ca2+]iand the expressions of caspases-3.nd-12 mRNA in *** pretreatments markedly depressed the neuronalabnormal elevation of [Ca2+]iand the overexpression of caspases-3.and-12 mRNAin a concentration-dependent ***:Res can attenuate the ratcortical neuronal apoptosis induced by OGD/*** mechanisms are,at leastpartly,due to the inhibition of the calcium overload and the overexpression ofcaspases-3.and-12 mRNA.

关键词： resveratrol oxygen-glucose deprivation/reperfusion brain ischemia apoptosis neurons

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Protective effects of icariin on neurons injured by cerebral ischemia/reperfusion

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中华医学杂志(英文版) 2005年第19期000卷 55-61页

作者： LI Li, ZHOU Qi-xin and SHI Jing-shanDepartment of pharmacology, zunyi medical college, {3. 563.03. ChinaDepartment of pharmacology,Chongqing medical University, Chongqing400016,China Department of pharmacology Zunyi Medical College Zunyi 563003 China Department of pharmacology Chongqing Medical University Chongqing 400016 China Department of pharmacology Chongqing Medical University Chongqing 400016 China Department of pharmacology Zunyi Medical College Zunyi 563003 China

Brain is very susceptible to hypo-glucose and hypoxia induced by ischemia/reperfusion and significant neuronal injuries during which large amount of free radicals is released. Therefore, in order to prevent neuron death and degeneration with dementia, many researchers have been engaging in seeking novel neuro-protective drugs that can decrease the neuron damages induced by ischemia/reperfusion or neutralize the neurotoxicity of free radicals. Unfortunately, remedies are rarely effective to reverse or prevent the pathophysiological course up to date.~ 1-6

关键词： icariin · neurons · cerebral ischemia · reperfusion

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Tissue and host tropism of influenza viruses:Importance of quantitative analysis

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Science China(Life Sciences) 2009年第12期52卷 1101-1110页

作者： ZHANG Hong1,2 1 Z-BioMed, Inc., Rockville, MD 20855, USA 2 Department of Respiratory Medicine, Affiliated Hospital of zunyi medical college, zunyi {3. China Z-BioMed Inc. Rockville MD 20855 USA Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi 563003 China

It is generally accepted that human influenza viruses preferentially bind to cell-surface glycoproteins/ glycolipids containing sialic acids in α2,6-linkage; while avian and equine influenza viruses preferentially bind to those containing sialic acids in α2,3.linkage. Even though this generalized view is accurate for H3.subtype isolates, it may not be accurate and absolute for all subtypes of influenza A viruses and, therefore, needs to be reevaluated carefully and realistically. Some of the studies published in major scientific journals on the subject of tissue tropism of influenza viruses are inconsistent and caused confusion in the scientific community. One of the reasons for the inconsistency is that most studies were quantitative descriptions of sialic acid receptor distributions based on lectin or influenza virus immunohistochemistry results with limited numbers of stained cells. In addition, recent studies indicate that α2,3. and α2,6-linked sialic acids are not the sole receptors determining tissue and host tropism of influenza viruses. In fact, determinants for tissue and host tropism of human, avian and animal influenza viruses are more complex than what has been generally accepted. Other factors, such as glycan topology, concentration of invading viruses, local density of receptors, lipid raft microdomains, coreceptors or sialic acid-independent receptors, may also be important. To more efficiently control the global spread of pandemic influenza such as the current circulating influenza A H1N1, it is crucial to clarify the determinants for tissue and host tropism of influenza viruses through quantitative analysis of experimental results. In this review, I will comment on some conflicting issues related to tissue and host tropism of influenza viruses, discuss the importance of quantitative analysis of lectin and influenza virus immunohistochemistry results and point out directions for future studies in this area, which should lead to a better understanding

关键词： tissue and host tropism influenza viruses sialic acid receptors quantitative analysis

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Inhibitory effect of ginsenoside Rb1 on calcineurin signal pathway in cardiomyocyte hypertrophy induced by prostaglandin F2α

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Acta Pharmacologica Sinica 2007年第8期28卷 1149-1154页

作者： Qing-song JIANG~2 Xie-nan HUANG Gui-zhong YANG~4 Xiao-yan JIANG~5 Qi-xin ZHOU~(2,6) ~2Department of pharmacology,Chongqing medical University,Chongqing 400016,China {3.,zunyi medical college,zunyi 563.03.China ~4Department of Biochemistry zunyi medical college,zunyi 563.03.China ~5Chengdu Vocational college of Agricultural Science and Technology,Chengdu 611100,China Department of pharmacology Chongqing Medical UniversityChongqing400016China Department of pharmacology Zunyi Medical CollegeZunyi 563003China Department ofBiochemistry Zunyi Medical CollegeZunyi 563003China

Aim:To examine the antihypertrophic effect of ginsenoside Rb1（Rb1）induced byprostaglandin F2α(PGF2α)in vitro and to investigate the possible mechanismsinvolved in the calcineurin（CAN）signal transduction ***:Thecardiomyocyte hypertrophy induced by PGF2αand the antihypertrophic effect ofRbiwere evaluated in primary culture by measuring the cell diameter,proteincontent,and atrial natriuretic peptide（ANP）mRNA *** and CaNmRNA expressions,CaN and its downstream effectors NFAT3.nd GATA4 proteinexpressions,and the intracellular free Ca2+concentration([Ca2+]i)were assayedby RT-PCR,Western blot,and fluorescent determination using Fura 2/AM,***:PGF2α（100 nmol/L）significantly increased the cardiomyo-cyte diameter,protein content and[Ca2+]i,and promoted ANP,CaN mRNA,andCaN/NFAT3.GATA4protein expressions,which were inhibited by either Rb1in aconcentration-dependent manner（50,100,and 200μg/mL）or L-arginine（1 mmol/L）.NG-nitro-L-arginine-methyl ester,a nitric oxide synthase inhibitor,could abolishthe effects of L-arginine,but failed to change the effects of Rb1in the ***:The present data implicate that Rb1attenuates cardiachypertrophy,the underlying mechanism may be involved in the inhibition of theCa2+-CaN signal transduction pathway.

关键词： Rb1 prostaglandin F2α cardiac hypertrophy calcineurin

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Conversion therapy for advanced cholangiocarcinoma in the era of molecular targeted therapy and immune therapy

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Hepatobiliary Surgery and Nutrition 2023年第5期12卷 772-775页

作者： zunyi Zhang Wei Zhang Hepatic Surgery Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Hubei Clinical Research Center for Hepatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Hubei Key Laboratory of Hepato-Pancreato-Biliary Diseases Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

Cholangiocarcinoma(CCA)encompasses a range of neoplasms,namely intrahepatic CCA,perihilar CCA,and distal CCA,which are distinguished by their anatomical *** cases where radical resection of CCA is feasible and the patient’s physical condition permits surgical intervention,it is advisable to proceed with surgical treatment(1).However,the insidious onset of the disease restricts surgical candidacy to only 20-3.%of patients(2,3..For those with unresectable CCAs,the recommended initial treatment is gemcitabine plus cisplatin(GemCis)chemotherapy(4).

关键词： Silmitasertib cholangiocarcinoma(CCA) conversion therapy

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Tanshinone prevents cancellous bone loss induced by ovariectomy in rats

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Acta Pharmacologica Sinica 2004年第05期25卷 136-142页

作者： Liao CUI2,3. Tie WU3. Yu-yu LIU3. Yi-feng DENG4, Chun-mei AI4, Huai-qing CHEN22Institue of Biomedical Engineering, West China medical Center, Sichuan University, Chengdu 610041, China 3.epartment of {3., 4Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong medical college, Zhanjiang 524023.China Institue of Biomedical Engineering West China Medical Center Sichuan University Department of pharmacology Guangdong Key Laboratory for Research and Development of Natural Drugs Guangdong Medical College

AIM: To investigate the skeletal effects of total tanshinone in ovariectomized rats by analyzing cancellous bonehistomorphometry of fourth lumbar vertebrae (LV4) and proximal tibial metaphyses (PTM). METHODS: Four-month-old Sprague-Dawley female rats were sham-operated and treated with vehicle or ovariectomized and treatedwith either vehicle, total tanshinone (200 mgkg-1d-1, equivalent to 3. gkg-1d-1 of tanshinone II A and 16 mgkg -1d-1 ofcryptotanshinone), or 17a-ethynylestradiol (3. gkg-1d-1 as positive treatment group) starting one day post-sur-gery for 10 weeks. Double in vivo fluorochrome labeling was administered to all rats. The undecalcified longitu-dinal LV4 and PTM sections were cut and stained with Goldners Trichrome (4-m thickness) or unstained (8-mthickness) for the bone histomorphometric analysis. RESULTS: A significant decrease in trabecular bone volume(BV/TV) and trabecular number (Tb.N) and a significant increase in osteoclast surface (OCS/BS) and mineralizingsurface (MS/BS) were found in both LV and PTM of vehicle-treated OVX rats compared with sham *** completely prevented the decreases in BV/TV and Tb.N and the increase in OCS/BS in the LV4, andpartially prevented the decreases in BV/TV and Tb.N in the PTM of OVX rats. In addition, tanshinone increasedtrabecular thickness (***) whereas it did not alter MS/BS. Moreover, tanshinone had no effect on uterine weightand body weight of OVX rats. Estrogen treatment increased BV/TV and Tb.N and decreased OCS/BS, but, alsomarkedly decreased MS/BS and increased uterine weight in OVX rats. CONCLUSION: The current study demon-strated that the adequate supply of tanshinone prevented OVX-induced cancellous bone loss in rats through inhibi-tion of elevated bone resorption.

关键词： tanshinone cryptotanshinone 2-fluoro-17alpha-ethynylestradiol ovariectomy bone and bones osteoclasts osteoporosis histocytochemistry morphology

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Icariin ameliorates learning and memory deficit induced by D-galactose in rats

Icariin ameliorates learning and memory deficit induced by D...

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中国药理学会第十次全国学术会议

作者： LI Fei,SHI Jing-shan (Department of pharmacology,zunyi medical college,{3.,Guizhou 563.03.China)

The effects of icariin from the Chinese medical herb Epimedium revicornum Maxim on spatial memory performances and expressions of BDNF and TrkB in hippocampus of rats were *** forty Sprague-Dawley rats were randomly d...

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长爪沙鼠体内周期型马来丝虫微丝蚴的季节周期性

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Current Zoology 1983年第2期29卷 180-184页

作者： Department of Parasitology, zunyi medical college, Guizhou 遵义医学院寄生虫学教研室

微丝蚴在外周血液中的出现,不仅有明显的日夜周期性,还有明显的季节周期性,后一现象不仅发生在马来丝虫(陆素筠,1956)或班氏丝虫的患者(Brengues等,1975),也见于有犬恶丝虫或匐形恶丝虫寄生的犬体(Kume和Ohishi,1965;Katamme等,1970;Aok... 详细信息

微丝蚴在外周血液中的出现,不仅有明显的日夜周期性,还有明显的季节周期性,后一现象不仅发生在马来丝虫(陆素筠,1956)或班氏丝虫的患者(Brengues等,1975),也见于有犬恶丝虫或匐形恶丝虫寄生的犬体(Kume和Ohishi,1965;Katamme等,1970;Aoki,1971;Cancrml,1975a、b;Sawyer,1975)。本文报道实验感染周期型马来丝虫的长爪沙鼠体内微丝蚴的季节周期性。

关键词： Meriones unguiculatus Brugia malayi Periodicity Microfilariae Rhythm

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周期型马来丝虫微丝蚴寿命的观察

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Current Zoology 1984年第1期30卷 63-66页

作者： Department OF PAEASITOLOGT, zunyi medical college, GUIZHOU 遵义医学院寄生虫学教研室

将实验感染周期型马来丝虫的长爪沙鼠的微丝蚴蚴阳性腹腔稀释液,移注于正常沙鼠腹腔内,微丝蚴除能在腹腔内长期生存外,还可出现于外周血液中,其在外周血液内末次阳性检出时间最长可超过3.周,在腹腔液内末次阳性检出时间最长为77周,故马... 详细信息

将实验感染周期型马来丝虫的长爪沙鼠的微丝蚴蚴阳性腹腔稀释液,移注于正常沙鼠腹腔内,微丝蚴除能在腹腔内长期生存外,还可出现于外周血液中,其在外周血液内末次阳性检出时间最长可超过3.周,在腹腔液内末次阳性检出时间最长为77周,故马来微丝蚴在沙鼠外周血液中的最长寿命不短于7.5月,而在腹腔液内的最长寿命可超过1.5年以上。

关键词： Meriones unguiculatus Brugia malayi Microfilaria Longevity Physiology.

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Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion

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Acta Pharmacologica Sinica 2004年第5期25卷 81-87页

作者： Jun-qiang SI2, Zhi-qin ZHANG, Chun-xia LI, Li-feng WANG, Yun-lei YANG3. Zhi-wang LI3.epartment of Physiology, medical college Shihezi University, Shihezi 83.002, China3.Now in Tongji medical college, Huazhong University of Sci-ence and Technology, Wuhan 43.03., China. Department of Physiology Medical College Shihezi University

AIM: To explore the modulatory effect of substance P (SP) on GABA-activated current of dorsal root ganglion(DRG) neurons in rat. METHODS: The whole-cell patch-clamp technique was used to record SP- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons. Drugs were applied by rapid solutionexchange. RESULTS: Application of SP (28/41, 68.5 %) and GABA (3./41, 88.2 %) could induce concentration-dependent inward current in some cells. SP-(10 mol/L) and GABA (100 mol/L)-activated inward currents were(24483. pA (n=9) and (1.80.5) nA (n=13., respectively. The majority of GABA-activated current had obviousthree processes, the peak value (Ip), the steady state (Iss) and the desensitization (Id). The desensitization of GABA-activated current was a biphasic process, including fast and slow desensitization. However, pre-application of SP(0.0011 mol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects were concentration-dependent. The inhibitory effect of SP on the peakvalue of GABA-activated current was more than the steady state of GABA-activated current. The inhibition ofGABA-activated current by SP (0.1 mol/L) was related to the time after application of SP, the inhibition of GABA-activated currents by SP reached the peak at about 4 min (49.8 %7.2 %, n=7, P<0.01) and took about 12 min toget a full recovery. The inhibition of GABA-activated currents by SP was almost completely removed after block-ade of PKC by H-7 with the re-patch clamp. CONCLUSION: Pre-application of SP exerts a more strong inhibitoryeffect on the peak value of GABA-activated current than the steady state of GABA-activated current.

关键词： patch-clamp techniques spinal ganglia substance P GABA protein kinase C

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