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Sodium butyrate-induced death-associated protein kinase expression promote Raji cell morphological change and apoptosis by reducing FAK protein levels

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Acta Pharmacologica Sinica 2007年第11期28卷 1783-1790页

作者： Hai-tao ZHANG~(2,5),Zhe-ling FENG~3,Jun WU~4,Ya-jun WANG~2,Xia GUO~2,Nian-ci LIANG~2,Zhen-yu ZHU~(3,5),Jian-quan MA~3~2 Institute of biochemistry and Molecular biology Guangdong medical college,Zhanjiang 524023,china ~3 department of biochemistry andmolecular biology.sums medical college of sums university guangzhou 510089,china ~4 Affiliated Hospital of Guangdong medicalcollege,Zhanjiang 524023,china ute of biochemistry and Molecular biology Guangdong Medical College Zhanjiang 524023 China. taohaizhang@***.

Aim:To investigate the role of death-associated protein kinase (DAPK) on theapoptosis of Raji cells induced by sodium ***:The apoptosis ofRaji cells were induced by sodium butyrate for 2,4,6,8,and 10 ***,theRaji cells were inhibited to adhere on culture flask by *** viabilitywas detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidemethod and the cell apoptosis percentage was estimated by flow *** focal adhesion kinase (FAK) expression were measured by Western *** sequence on the C-terminal of DAPK,which can suppress the function ofDAPK,was tranfected into the Raji cells to investigate whether the C-terminal ofDAPK could inhibit the apoptosis of Raji cells induced by sodium ***:After being treated with sodium butyrate,the Raji cells expressed DAPK anddisplayed many protrusions to adhere onto the culture *** Raji cells weresusceptive to apoptosis when they were inhibited adhesion by *** time,the cell viability decreased,the cell apoptosis percentage increased andthe protein levels of total FAK were *** Raji cells,which were trans-fected with the coding region on the C-terminal of DAPK,sustained apoptosisand the FAK protein level when treated with sodium ***:So-dium butyrate induced DAPK *** caused the Raji cells to display manyprotrusions all around the cells and adhere onto the culture *** expres-sion prompted apoptosis by reducing the FAK protein level in sodium butyrate-induced Raji cells.

关键词： Raji cells death-associated protein kinase apoptosis sodium butyrate focal adhesion kinase

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Parameter identification theory of a complex model based on global optimization method

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Science china(Physics,Mechanics & Astronomy) 2008年第11期51卷 1722-1732页

作者： QU Jie1, JIN QuanLin2 & XU BingYe3 1 college of Automotive Engineering, South china university of Technology, guangzhou 510640, china 2 Beijing Research Institute of Mechanical and Electrical Technology, Beijing 100083, china 3 department of Engineering Mechanics, Tsinghua university, Beijing 100084, china 1. college of Automotive Engineering South China University of Technology Guangzhou 510640 China 2. Beijing Research Institute of Mechanical and Electrical Technology Beijing 100083 China 3. department of Engineering Mechanics Tsinghua University Beijing 100084 China

With the development of computer technology and numerical simulation technology, computer aided engineering (CAE) technology has been widely applied to many fields. One of the main obstacles, which hinder the further application of CAE technology, is how to successfully identify the parameters of the selected model. An elementary framework for parameter identification of a complex model is provided in this paper. The framework includes the construction of objective function, the design of the optimization method and the evaluation of the identified results, etc. The parameter identification process is described in this framework, taking the parameter identification of the superplastic constitutive model considering grain growth for Ti-6Al-4V at 927°C as an example. The objective function is the weighted quadratic sums of the difference between the experimental and computational data for the stress-strain relationship and the grain growth relationship; the designed optimization method is a hybrid global optimization method, which is based on the feature of the objective function and incorporates the strengths of genetic algorithm (GA), the Levenberg-Marquardt algorithm and the augmented Gauss-Newton algorithm. The reliability evaluation of parameter identification result is made through the comparison between the calculated and experimental results and between the theoretical values of the parameters and the identified ones.

关键词： constitutive model parameter identification theory inverse analysis global optimization

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Copper transportion of WD protein in hepatocytes from Wilson disease patients in vitro

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World Journal of Gastroenterology 2001年第6期7卷 846-851页

作者： Guo-Qing Hou~1 Xiu-Ling Liang~2 Rong Chen~2 Li-Wen Tang~3 Ying Wang~2 Ping-Yi Xu~2 Ying-Ru Zhang~2 Cui-Hua Ou~2 1 department of Neurology.guangzhou First Municipal People’s Hospital,guangzhou medical college,guangzhou 510180,Guangdong Province.china2 department of Neurology.First Affiliated Hospital.Sun Yat-Sen university of medical Sciences.guangzhou 510080.Guangdong Province.china3 department of Pharmacology,university of Kentucky.Lexington,KY 40506.USA department of Neurology Guangzhou First Municipal People's Hospital Guangzhou Medical College Guangdong Province China. spring-hua@***

AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD). METHODS: WD copper transporting properties in some organelles of the cultured hepatocytes were studied from WD patients and normal *** cultured hepatocytes were incubated in the media of copper 15 mg x L(-1) only, copper 15 mg x L(-1) with vincristine (agonist of P-type ATPase) 0.5mg x L(-1), or copper 15 mg x L(-1) with vanadate (antagonist of P-type ATPase) 18.39 mg x L(-1) separately. Microsome (endoplasmic reticulum and Golgi apparatus), lysosome, mitochondria, and cytosol were isolated by differential centrifugation. Copper contents in these organelles were measured with atomic absorption spectrophotometer, and the influence in copper transportion of these organelles by vanadate and vincristine were comparatively analyzed between WD patients and controls. WD copper transporting P-type ATPase was detected by SDS-PAGE in conjunction with Western blot in liver samples of WD patients and controls. RESULTS: The specific WD proteins (M(r)155,000 lanes) were expressed in human hepatocytes, including the control and WD patients. After incubation with medium containing copper for 2 h or 24 h, the microsome copper concentration in WD patients was obviously lower than that of controls, and the addition of vanadate or vincristine would change the copper transporting of microsomes obviously. When incubated with vincristine, levels of copper in microsome were significantly increased, while incubated with vanadate, the copper concentrations in microsome were obviously decreased. The results indicated that there were WD proteins, the copper transportion P-type ATPase in the microsome of hepatocytes. WD patients possessed abnormal copper transporting function of WD protein in the microsome, and the agonist might correct the defect of copper transportion by promoting the activity of copper transportion P-type ATPase. CONCLUSION: Cop

关键词： Adenosinetriphosphatase Adolescent Adult Cation Transport Proteins Copper Female Hepatocytes Hepatolenticular Degeneration Humans Male Research Support, Non-U.S. Gov't

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纪念活动与毛泽东思想大众化——以毛泽东诞辰110周年纪念活动为考察对象

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重庆教育学院学报 2011年第4期24卷 42-46页

作者：朱斌华南师范大学增城学院政治与行政学院广州511363

12月26日是毛泽东的诞辰日,对毛泽东诞辰110周年纪念一方面充分表达了人民对毛泽东的无限怀念之情,充分展现了对毛泽东研究的成果;另一方面,使得毛泽东思想深入人心,推动毛泽东思想的大众化进程。本文总结毛泽东诞辰110周年纪念活动的... 详细信息

12月26日是毛泽东的诞辰日,对毛泽东诞辰110周年纪念一方面充分表达了人民对毛泽东的无限怀念之情,充分展现了对毛泽东研究的成果;另一方面,使得毛泽东思想深入人心,推动毛泽东思想的大众化进程。本文总结毛泽东诞辰110周年纪念活动的具体纪念方式,揭示了毛泽东诞辰周年纪念活动对毛泽东思想大众化的作用,并总结了纪念活动对马克思主义大众化的启示。

关键词：毛泽东诞辰周年纪念思想大众化

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