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Antagonist peptides of human interferon-α2b isolated from phage display library inhibit interferon induced antiviral activity

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Acta Pharmacologica Sinica 2006年第8期27卷 1044-1050页

作者： Wang TIAN~1 Gang BAI~(2,3) Zheng-he LI~2 wen-bo YANG~2 ~1department of microbiology,college of Life Sciences,Nankai University,Tianjin 300071,china." 2college of Life Sciences,Nankai University,Tianjin 300071,china department of microbiology College of Life Sciences Nankai University Tianjin 300071 China college of Life Sciences Nankai University Tianjin 300071 China

Aim:To screen human interferon(IFN)-α2b antagonist peptides from a phagedisplayed heptapeptide ***:WISH cells and polyclonal anti-IFN-α2b antibodies were used to select IFN receptor-binding peptides from a phagedisplayed heptapeptide *** specific binding of phage clones was exam-ined by phage ELISA and *** specific binding activitiesof synthetic peptides to WISH cells were detected by competition *** synthetic peptides to IFN-induced antiviral activity were analyzed by evaluat-ing the cytopathic effect(CPE)using the MTT ***:Twenty-threepositive clones were obtained after seven rounds of *** clones wererandomly picked from the positive clones and were *** correspond-ing amino acid sequences suggested 3 groups homologous to the 3 domains ofIFN-α2b,defined by residues 24-41,43-49,and 148-158 of IFN-α*** theypresented as corresponding to IFN receptor-binding domains,AB loop and Ehelix,clone № 26 and 35 were chosen for further characterization and shown tobind to WISH *** peptides corresponding to clone № 26 and 35,desig-nated SP-7(SLSPGLP)and FY-7(FSAPVRY)were shown to compete with GFP-IFN-α2b for binding to its receptor and to inhibit the IFN-α2b-induced ***:Both IFN-α2b antagonist peptides,SP-7 and FY-7,wereable to inhibit the IFN-induced antiviral activity,and could be helpful in laying thefoundation for the molecular mechanism of the interaction between IFN and itsreceptor.

关键词： interferon-α2b antagonist peptide phage display type Ⅰ IFN receptor cell selection

Expression of vascular endothelial growth factor and its role in oncogenesis of human gastric carcinoma

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World Journal of Gastroenterology 2001年第4期7卷 500-505页

作者： Du-Hu Liu Xue-Yong Zhang Dai-ming Fan Yu-Xin Huang Jin-Shan Zhang Wei-Quan Huang Yuan-Qiang Zhang Qing-Sheng Huang wen-Yu Ma Yu-Bo Chai ming Jin Institute of Digestive Disease,Xijing Hospital,~2 department of Gastroenterology,Tangdu Hospital,~3department of Histology and Embryology,~4 department of microbiology,~5 department of Biochemistry,Fourth Military medical University,Xi’an 710033,Shaanxi Province,china Institute of Combined Injury Third Military Medical University Chongqing 400038 China. liuduhu@***

AIM: To establish the role of vascular endothelial growth factor (VEGF) in the oncogenesis of human gastric carcinoma more directly. METHODS: The expression of VEGF and its receptor kinase-domain insert containing receptor (KDR) in human gastric cancer tissue were observed by immunohistochemical staining. VEGF levels were manipulated in human gastric cancer cell using eukaryotic expression constructs designed to express the complete VEGF(165) complimentary DNA in either the sense or antisense orientation. The biological changes of the cells were observed in which VEGF was up-regulated or down-regulated. RESULTS: VEGF-positive rate was 50%, and VEGF was mainly localized in the cytoplasm and membrane of the tumor cells, while KDR was mainly located in the membrane of vascular endothelial cells in gastric cancer tissues and peri-cancerous tissue. In 2 cases of 50 specimens, the gastric cancer cells expressed KDR, localized in both the cytoplasm and membrane. Introduction of VEGF(165) antisense into human gastric cancer cells (SGC-7901, immunofluorescence intensity, 31.6%)) resulted in a significant reduction in VEGF-specific messenger RNA and total and cell surface VEGF protein (immunofluorescence intensity, 8.9%) (P2.88 mm3), whereas up-regulation of VEGF resulted in increased xenografted tumor size (at 33 days postimplantation, tumor volume: 2350.50 +/- 637.70 mm3) (P<0.05 vs control SGC-7901 group). CONCLUSION: This study provides direct evidence that VEGF plays an important role in the oncogenesis of human gastric cancer.

关键词： Gene Expression Regulation, Neoplastic Adult Aged Animals Cell Division Cloning, Molecular DNA, Antisense DNA, Complementary Endothelial Growth Factors Endothelium, Vascular Female Humans Lymphokines Male Mice Mice, Nude Middle Aged Neovascularization, Pathologic Receptor Protein-Tyrosine Kinases Receptors, Growth Factor Receptors, Vascular Endothelial Growth Factor Stomach Neoplasms Transfection Tumor Cells, Cultured Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors

Cascade electrolysis and thermocatalysis: a reliable system for upgrading C1 to C4 hydrocarbons

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Rare Metals 2024年第1期43卷 410-412页

作者： Chao-Yue Sun wen Li Hai-Qing Wang Institute for Advanced Interdisciplinary Research(iAIR) College of Chemistry and Chemical EngineeringUniversity of JinanJinan 250022China

The carbon cycle is important for maintaining a stable climate and carbon balance on Earth. Renewable-electricitydriven upcycling of carbon dioxide (CO_(2)) into value-added multi-carbon molecules is a potentially sus... 详细信息

关键词： cycling hydrocarbons enable

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Inhibition of ATP-induced calcium influx in HT4 cells by glucocorticoids:involvement of protein kinase A

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Acta Pharmacologica Sinica 2005年第2期26卷 199-204页

作者： Jian-zhong HAN,wen LIN~2,Yi-zhang CHEN~3Neuroscience Research Institute and department of Neurobiology,~2department of Pharmacology,college of Pharmacy,Second Military medicalUniversity,Shanghai 200433,china NeuroscienceResearchInstituteanddepartmentofNeurobiology CollegeofPharmacySecondMilitaryMedicalUniversitySha

Aim:In our previous observations,adenosine triphosphate(ATP)was found toevoke immediate elevations in intracellular free calcium concentration([Ca2+]i)in HT4 neuroblastoma cells of *** tried to see if a brief pretreatment ofglucocorticoids could inhibit the Ca2+response and reveal the underlying signal-ing ***:Measurement of[Ca2+]iwas carried out using thedual-wavelength fluorescence method with Fura-2 as the ***:Pre-incubation of HT4 cells for 5 min with corticosterone(B)or bovine serum albu-min conjugated corticosterone(B-BSA)inhibited the peak[Ca2+]iincrements in aconcentration-dependent *** and dexamethasone had a similar action,while deoxycorticosterone and cholesterol were *** extracellularCa2+influx and intemal Ca2+release contributed to ATP-induced[Ca2+]*** brief treatment with only B attenuated Ca2+***,the[Ca2+]ielevation induced by the P2X receptor agonist adenosine 5’-(β,γ-methylene)triph-osphate(β,γ-meATP)was also *** rapid inhibitory effect of B canbe reproduced by forskolin 1 mmol/L and blocked by H89 20 mmol/*** glucocorticoid receptor antagonist mifepristone nor protein kinase C in-hibitors influenced the rapid action of ***:Our results suggest thatglucocorticoids modulate P2X receptor-medicated Ca2+influx through a mem-brane-initiated,non-genomic and PKA-dependent pathway in HT4 cells.

关键词： calcium glucocorticoids adenosine triphosphate protein kinase C protein kinase A

Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) wen-Tao PENG~1 Yang LIU~1 Ting WU~2 Yu-Feng YAO~1 ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of Molecular microbiology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,china.2 department of Medicinal Chemistry,School of Pharmacy,Fudan University,Shanghai 200032,china.3 State Key Laboratory of Genetic Engineering & department of microbiology,School of Life Science,Fudan University,Shanghai 200433,china.4 Laboratory of Disease and Health Genomics,Chinese National Human Genome Center at Shanghai,Shanghai 201203,china Laboratory of Molecular microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering & department of microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

Overview of ferroptosis and pyroptosis in acute liver failure

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World Journal of Gastroenterology 2024年第34期30卷 3856-3861页

作者： Ya-wen Sun Bo-wen Zhao Hai-Fang Li Guang-Xiao Zhang college of Life Sciences Shandong Agricultural UniversityTai’an 271018Shandong ProvinceChina

In this editorial,we comment on the article by Zhou et al published in a recent *** specifically focus on the crucial roles of ferroptosis and pyroptosis in acute liver failure(ALF),a disease with high mortality *** is the result of increased intracellular reactive oxygen species due to iron accumulation,glutathione(GSH)depletion,and decreased GSH peroxidase 4 activity,while pyroptosis is a procedural cell death mediated by gasdermin D which initiates a sustained inflammatory *** this review,we describe the characteristics of ferroptosis and pyroptosis,and discuss the involvement of the two cell death modes in the onset and development of ***,we summarize several interfering methods from the perspective of ferroptosis and pyroptosis for the alleviation of *** observations might provide new targets and a theoretical basis for the treatment of ALF,which are also crucial for improving the prognosis of patients with ALF.

关键词： Acute liver failure Ferroptosis Pyroptosis Glutathione peroxidase 4 Gasdermin D

pH-Dependent Stability of EGX, A Multi-functional Cellulase from Mollusca, Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 603-608页

作者： wen-Ying LI2, Ji WANG1, Yan-Hong LI1, ming DING1, Gen-Jun XU1,3, Lan-Ying LIU2, and Fu-Kun ZHAO1,3* 1Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china.2college of Life Science, Jilin University, Changchun 130023, china.college of Life Science, Zhejiang University of Science and Technology, Hangzhou 310018, china Key Laboratory ofProteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

The cellulase activity and stability of EGX, a multi-functional cellulase previously purified from the mollusca Ampullaria crossean, was systematically studied under different pH. The pH induced con- formation and stability change of EGX have been investigated by using the intrinsic fluorescence, ANS fluorescence and CD spectrum. It has been found that the conformation and activity of this cellulase were strongly dependent on the pH. EGX was stable for both the enzyme activity and the conformation from pH 5.6 to pH 7.4. As shown by intrinsic and ANS fluorescence, no red shift of emission maximum occurred and a negligible intensity change was observed at pH 5.6–7.4. The activity of EGX remained about 80% in pH 5.6–7.4 and obviously decreased out of side the pH range. Urea-induced changes in EGX at pH 5.4 and pH 8.0 were measured by intrinsic fluorescence and CD spectrum. At pH 5.4, a significantly red shift of emission maximum occurred when the concentration of urea was 5 M compared to the concentration was 3 M at pH 8.0. The α-helix at pH 5.4 was 40.51% in the absence of urea and 31.04% in the presence of 4 M urea. At pH 8.0 the α-helix was 7.23% in the presence of 4 M urea. The data indicated that EGX was much susceptible to urea-induced unfolding at pH 8.0 and much stable at pH 5.4. The greater pH dependent stability of EGX may allow the enzyme to adequately catalyze the hydrolysis of cellulosic materials under natural or industrial extreme conditions.

关键词： multi-functional cellulase stability Ampullaria crossean fluorescence spectroscopy pH-dependent structural change CD spectrum

EFFECT OF EPIMEDIUM FLAVONE TOTAL(EFT) ON THE IMMUNOFUNCTION OF EHRLICH ASCITES CANCER(EAC) BEARING MICE

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Journal of Pharmaceutical Analysis 1999年第2期13卷 129-132页

作者： Hao Binghua,Tao ming,He Liduan,Xue Wanli, Li Xuanshe,Zhang Jingtian department of microbiology and immunology, college of Public Health, Xi′an medical University, Xi′an 710061 Dept. of microbiology and immunology College of Public Health Xi'an Medical University Xi'an 710061 China

Effect of Epimedium Flavone Total(EFT) on the immunofunction of mice which was burdened with Ehrlich Ascites Cancer (EAC) was studied. The results showed that EFT could increase phagocytosis activity of macrophagocyte (Pming Cell (PFC) signficantly (P<0 05), raise the ratio of spleen to body (P<0 05) of EAC bearing mice. EFT may be enhance burdened mice's immunofunction. Low dosage can improve sero antibody level. Effect on regulating cellular immunofunction are related to dosage. EFT may be inhibit the growth of EAC cells to some degree.

关键词： Epimedium Flavone Total (EFT) burdened mice immunofunction rate of tumor growth inhition(TGI)

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Anti-ageing Effects of Mushroom Extracts Obtained Using the Natural Form Extraction(NFE) Method

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食用菌学报 2008年第1期 47-50页

作者： YANG Xiaobing1,XIA Fengna1,ZHANG Song2,LIU Jinqing21guangdong Yuewei Edible Fungi Technology Co.Ltd,guangdong Institute of microbiology,Guangzhou,guangdong 510070,china.2college of Life Sciences,South china.Normal University,Guangzhou,guangdong 510631,china

A total of 23 mushroom extracts were prepared using the newly developed Natural Form Extraction(NFE) method and screened for anti-ageing activity in Drosophila *** amounts of extractives obtained from broken-walled spores of ***,and from *** and *** fruit bodies,were markedly higher when the NFE method was adopted compared with the more conventional Thermal Water Extraction(TWE) ***,in the case of ***,the Drosophila longevity prolongation rate for extracts prepared using the NFE method was almost three times *** dosage tests revealed that *** extract added to the basic Drosophila diet at a concentration of 20 g/L was most effective in prolonging the longevity of both male and female populations(prolongation rates of 43.2% and 20.3% for male and female populations,respectively).In the case of *** extract,5 g/L and 20 g/L dosages prolonged longevity in male Drosophila populations to a similar degree whereas 20 g/L was most effective in female *** the 23 mushroom materials tested,extracts of ***,***,Yuewei *** spores,***,***,*** and *** exhibited significant Drosophila anti-ageing activity,especially in the case of male populations.

关键词： Mushroom anti-ageing Natural Form Extraction Method bioactive extract