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Cloning and characterization of two putative seven-transmembrane receptor genes from cotton

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Progress in Natural Science:Materials International 2008年第10期18卷 1225-1231页

作者： Peng Gao Piming Zhao Juan Wang Haiyun Wang Guiling Wang Guixian Xia national Key Laboratory of plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Datun Road,Chaoyang District,beijing 100101,china national center for plant gene research,beijing 100101,china national Key Laboratory of plant Genomics Institute of Microbiology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 China national center for plant gene research Beijing 100101 China

Using rapid amplification of cDNA ends (RACE)-PCR,two full-length cDNAs encoding putative seven-transmembrane receptors (designated Gh7TMpRI and Gh7TMpR2) were cloned from cotton *** blot and an ApaL1 restriction site polymorphism analyses revealed that Gh7TMpRI was derived from the ancestral A diploid genome,while Gh7TMpR2 was from the D subgenome. Northern blot hybridization indicated that both Gh7TMpR1 and GhTTMpR2 were expressed preferentially in the elongation phase of fiber *** of the Gh7TMpRl proteins were located within the membrane structure and displayed a punctuate pattern of *** of Gh7TMpR1 in fission yeast disrupted the polar growth and caused the formation of rounded cells. These results suggest that GhT7MpRl may play a critical role in cotton fiber development,perhaps as a signaling receptor that is involved in controlling fiber elongation.

关键词： Cotton Seven-transmembrane protein Fiber elongation Alloalleles

Human induced pluripotent stem cell-derived therapies for regeneration after central nervous system injury

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Neural Regeneration research 2025年第11期20卷 3063-3075页

作者： Stephen Vidman Yee Hang Ethan Ma Nolan Fullenkamp Giles W.plant Department of Neuroscience Ohio State UniversityColumbusOHUSA

In recent years,the progression of stem cell therapies has shown great promise in advancing the nascent field of regenerative *** the non-regenerative nature of the mature central nervous system,the concept that“blank”cells could be reprogrammed and functionally integrated into host neural networks remained *** work has also demonstrated the ability of such cells to stimulate intrinsic growth programs in post-mitotic cells,such as *** embryonic stem cells demonstrated great potential in treating central nervous system pathologies,ethical and technical concerns *** barriers,along with the clear necessity for this type of treatment,ultimately prompted the advent of induced pluripotent stem *** advantage of pluripotent cells in central nervous system regeneration is multifaceted,permitting differentiation into neural stem cells,neural progenitor cells,glia,and various neuronal *** precise spatiotemporal application of extrinsic growth factors in vitro,in addition to microenvironmental signaling in vivo,influences the efficiency of this directed *** the pluri-or multipotency of these cells is appealing,it also poses the risk of unregulated differentiation and teratoma *** of the neuroectodermal lineage,such as neuronal subpopulations and glia,have been explored with varying degrees of *** the risk of cancer or teratoma formation is greatly reduced,each subpopulation varies in effectiveness and is influenced by a myriad of factors,such as the timing of the transplant,pathology type,and the ratio of accompanying progenitor ***,successful transplantation requires innovative approaches to develop delivery vectors that can mitigate cell death and support ***,host immune responses to allogeneic grafts must be thoroughly characterized and further developed to reduce the need for *** to a clinical setting wi

关键词： axon regeneration central nervous system regeneration induced pluripotent stem cells neurotrauma regenerative medicine spinal cord injury stem cell therapy

Expression and functional analysis of the rice plasma-membrane intrinsic protein gene family

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Cell research 2006年第3期16卷 277-286页

作者： Lei Guo~1 Zi Yi Wang~1 Hong Lin~1 Wei Er Cui~1 Jun Chen~1 Meihua Liu~1 Zhang Liang Chen~(1,2) Li Jia Qu~(1,2) Hongya Gu~(1,2)1 Peking-Yale Joint research center for plant Molecular genetics and AgroBiotechnology,national Laboratoryfor Protein Engineering and plant genetic Engineering,College of Life Sciences,Peking University,beijing 100871,china 2 The national plant gene research center,beijing 100101,china Peking-YaleJointresearchcenterforplantMoleculargeneticsandAgroBiotechnology NationalLaboratoryforProteinEngineeringandPlantGeneticEngineeringCollegeofLifeSciencesPekingUniversityBeijing100871China

Plasma membrane intrinsic proteins (PIPs) are a subfamily of aquaporins that enable fast and controlled translocationof water across the *** this study,we systematically identified and cloned ten PIP genes from *** on thesimilarity of the amino acid sequences they encoded,these rice PIP genes were classified into two groups and designatedas OsPIPI-1 to OsPIP1-3 and OsPIP2-1 to OsPIP2-7 following the nomenclature of PIP genes in ***-PCR analysis identified three root-specific and one leaf-specific OsPIP ***,the expression profile ofeach OsPIP gene in response to salt,drought and ABA treatment was examined in *** on transgenic plantsover-expressing of either OsPIP1 (OsPIPI-1) or OsPIP2 (OsPIP2-2) in wild-type Arabidopsis,showed enhanced toler-ance to salt (100 mM of NaCl) and drought (200 mM of mannitol),but not to salt treatment of higher concentration (150mM of NaCl).Taken together,these data suggest a distinct role of each OsPIP gene in response to different stresses,andshould add a new layer to the understanding of the physiological function of rice PIP genes.

关键词： aquaporins PIP quantitative RT-PCR Oryza sativa organ-specific

Isolation and annotation of 10828 putative full length cDNAs from indica rice

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Science china(Life Sciences) 2005年第5期48卷 445-451页

作者： XIE Kabin1, ZHANG Jianwei1, XIANG Yong1, FENG Qi2, HAN Bin2, CHU Zhaohui1, WANG Shiping1, ZHANG Qifa1 & XIONG Lizhong1 1. national Key Laboratory of Crop genetic Improvement and national center of plant gene research (Wuhan), Huazhong Agricultural University, Wuhan 430070, china 2. national center for gene research, Chinese Academy of Sciences, Shanghai 200233, china 1. national Key Laboratory of Crop genetic Improvement and national center of plant gene research (Wuhan) Huazhong Agricultural University 430070 Wuhan China 2. national center for gene research Chinese Academy of Sciences 200233 Shanghai China

We reported the isolation and identification of 10828 putative full-length cDNAs (FL-cDNA) from an indica rice cultivar, Minghui 63, with the long-term goal to isolate all full-length cDNAs from indica genome. Comparison with the databases showed that 780 of them are new rice cDNAs with no match in japonica cDNA database. Totally, 9078 of the FL-cDNAs contained predicted ORFs matching with japonica FL-cDNAs and 6543 could find homologous proteins with complete ORFs. 53% of the matched FL-cDNAs isolated in this study had longer 5′UTR than japonica FL-cDNAs. In silico mapping showed that 9776 (90.28%) of the FL-cDNAs had matched genomic sequences in the japonica genome and 10046 (92.78%) had matched genomic se-quences in the indica genome. The average nucleotide sequence identity between the two sub-species is 99.2%. A majority of FL-cDNAs (90%) could be classified with GO (gene ontology) terms based on homology proteins. More than 60% of the new cDNAs isolated in this study had no homology to the known proteins. This set of FL-cDNAs should be useful for functional ge-nomics and proteomics studies.

关键词： Oryza sativa L., indica, functional genomics.

Effects of elevated CO_2 on growth and carbon partitioning in rice

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Chinese Science Bulletin 1998年第23期43卷 1982-1986页

作者： LIN Weihong\+1 and WANG Dali\+2 1. Department of plant Nutrition, china Agricultural University, beijing 100094, china 2. Department of Systems Ecology, research center for Eco_Environmental Science, Chinese Academy of Sciences, beijing 100085, china Department of plant Nutrition China Agricultural University Beijing China Department of Systems Ecology Research Center for Eco-Environmental Science Chinese Academy of Sciences Beijing China

Rice (\%Oryza sativa\% cv. Jindao 1187) was grown in open_top chambers which contained ambient and enriched CO\-2. CO\-2 elevation stimulated rice tillering during early vegetative stage. However, panicle dry weight per plant did not change at maturity stage. Root biomass was enhanced by high CO\-2. Root / shoot ratio was increased under high CO\-2 at maturity, indicating more carbon allocation to the below_bround part in rice under high CO\-2.

关键词： CO 2 elevation growth carbon partitioning rice (\%Oryza sativa cv\%. Jindao 1187).

Growth phase-dependent expression of proteins with decreased plant-specific N-glycans and immunogenicity in tobacco BY2 cells

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Science china(Life Sciences) 2009年第8期52卷 739-746页

作者： YIN BoJiao1,2,ZHENG NuoYan1,2,LI Yin1,TANG SanYuan2,LIANG LiMing1 & XIE Qi1,2 1 State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen(Zhongshan) University,Guangzhou 510275,china 2 State Key Laboratory of plant Genomics,national Centre for plant gene research,Institute of genetics and Developmental Biology,Chinese Academy of Sciences,beijing 100101,china State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen (Zhongshan) University Guangzhou 510275 China State Key Laboratory of plant Genomics National Centre for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

plants possess some desirable characteristics to synthesize recombinant glycoproteins for pharma-ceutical ***,the mammalian glycoproteins produced in plants are somewhat different from their natural counterparts in terms of *** immunogenicity of plant-specific glyco-epitopes is the major concern in human ***,the distribution of N-glycans in different growth phases of tobacco BY2 cells and their immunogenicity in mice were *** was ob-served that the percentage of β1,2-xylose and α1,3-fucose in proteins of growing cells increased and the corresponding protein extracts caused accelerated immune response in *** on this observation,the recombinant erythropoietin in BY2 cells was expressed and characterized,and Western blot analysis showed that the recombinant erythropoietin contained a relatively small amount of plant-specific glyco-epitopes in the early phase of culture *** study may provide a simple but effective strategy for the production of therapeutic glycoproteins with human-like N-glycan structures in plant hosts to avoid a great allergenic risk.

关键词： BY2 cell,N-glycans,protein expression immunogenicity

Identification of genes contributing to quantitative disease resistance in rice

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Science china(Life Sciences) 2010年第11期53卷 1263-1273页

作者： KOU YanJun, LI XiangHua, XIAO JingHua & WANG ShiPing national Key Laboratory of Crop genetic Improvement, national center of plant gene research (Wuhan),Huazhong Agricultural University, Wuhan 430070, china 1. national Key Laboratory of Crop genetic Improvement National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China

Despite the importance of quantitative disease resistance during a plant’s life, little is known about the molecular basis of this type of host-pathogen interaction, because most of the genes underlying resistance quantitative trait loci (QTLs) are unknown. To identify genes contributing to resistance QTLs in rice, we analyzed the colocalization of a set of characterized rice defense-responsive genes and resistance QTLs against different pathogens. We also examined the expression patterns of these genes in response to pathogen infection in the parents of the mapping populations, based on the strategy of validation and functional analysis of the QTLs. The results suggest that defense-responsive genes are important resources of resistance QTLs in rice. OsWRKY45-1 is the gene contributing to a major resistance ***,OsGH3-1,and OsGLP members on chromosome 8 contribute alone or collectively to different minor resistance QTLs. These genes function in a basal resistance pathway or in major disease resistance gene-mediated race-specific pathways.

关键词： quantitative disease resistance QTL defense-responsive gene rice

Scale correlation between vegetation and soil in larch forest, NE china

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Journal of Environmental Sciences 2001年第1期13卷 51-57页

作者： MA Ke ming 1, ZU Yuan gang 2, Michel Godron 3 (1. Reserch center for Eco Environmental Sciences, Chinese Academy of Sciences, beijing 100085, china. E mail: mkm@*** 2. Open research Laboratory of Forest plant Ecology, Northeast Fo research center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China. mkm@***

After the analysis on the linages of species, the fractal dimensions of vegetation and soil in a managed larch(Larix gmelini) forest in Daxingan Mountains, NE china were estimated separately, and their scale correlation was discussed. (1) The dominant species of the larch forest, larch and the important accompany species, birch (Betula platyphylla) were homogeneously distributed along the transect. The other species were heterogeneous, and can be divided into three groups located on the right, left and central parts of the transect respectively. The transect can be separated into two parts at the position for the 29th quadrat(580m). (2) The fractal dimension in the large scale range from 200 to 400m was lower than that in the small scale range from 0 to 200m in the forest, indicating the different variation regularities of spatial heterogeneity in different scale ranges. The inflection point at 200m was one of the key scales of spatial hierarchy of the larch forest. (3) The scale variation of the forest was correlated with that of soil pH on large scales, which reflected the indirect control of the dominant species to the spatial pattern and species distribution of understorey by changing soil acidity in the forest and the indirect effects of forest management.

关键词： fractal dimension larch forest scale variation soil spatial heterogeneity species linkX

PAT: waking up a lazy sleeping beauty

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Cell research 2007年第5期17卷 387-388页

作者： Xiangdong Fu Jianru Zuo, national center for plant gene research, Institute of genetics and Developmental Biology, Chinese Academy of Sciences, beijing 100101, china. xdfu@*** nationalcenterforplantgeneresearch InstituteofGeneticsandDevelopmentalBiologyChineseAcademyofSciencesBeijing100101China

Once upon a time, there was an unusual corn; and un-like its normal siblings, its stalk grew towards the groundrather than in an upward direction. The sleeping stature ofthe corn was portrayed in early 1930s as "lazy" by Jenkinsand Gerhardt [1]. Later, lazy appeared to be a fashionin the plant kingdom, found in rice, barley, tomato andseveral other species [2]. These seemingly magic spell-chanted plants have drawn considerable interests of plantbiologists and breeders for many decades. To breeders, thelazy phenotype, viewed as tillering or branching anglesof stems (or termed as culms in crop plants), represents akey characteristic important for controlling photosynthesisefficiency and planting density, and thus a crucial factor indetermining crop production [6]. Previous physiologicalstudies suggested that the prostrate or lazy phenotype wasattributed, at least in part, to the loss of gravitropism. Forexample, the rice lazyl plants are ageotropic, whereas wildtype (WT) plants are negatively geotropic [2]. Gravity plays a major role in plant morphogenesis bydetermining the directional growth of plant organs. Upongermination, shoots grow upward, capturing light, and bycontrast, roots penetrate the soil and grow downward, tak-

关键词： waking up a lazy sleeping beauty PAT