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Cellular & Molecular immunology 2005年第6期2卷 467-471页

作者： Ming Li~1 Yuanchao Zhang~(1,2) ~1department of rheumatology and immunology,shandong provincial hospital,shandong university,jinan 250021,china.~2department of rheumatology and immunology,shandong provincial hospital,shandong university,jinan 250021,shandong,china. department of rheumatology and immunology Shandong Provincial Hospital Shandong University Jinan 250021 China

CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells （PBMCs） stimulated with phytohemagglutinin （PHA, 50 μg/ml） were treated by CD134 mAb （1μg/ml, 5 μg/ml, 10 μg/ml） for 6 h, 12 h, 24 h and 48 h. The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction （RT-PCR） technique. Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1 μg/ml to 5 μg/ml and dropped down to its minimum on 24 h （p 〈 0.05）. The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5 μg/ml. In conclusion, CD134 mAb can inhibit perforin expression, which may enhance the ability of T cells for survival. Cellular ＆ Molecular immunology. 2005;2（6）： 467-471.

关键词： CD134 perforin phytohemagglutinin

Zinc fingers and homeoboxes 2 involves in oncogenic activation of glypican 3 in human hepatocellular carcinoma

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Zinc fingers and homeoboxes 2 involves in oncogenic activati...

Therapeutic Characterization of ^(131)I-Labeled Humanized Anti-B7-H3 Antibodies for Radioimmunotherapy for Glioblastoma

2012全国临床微生物与感染免疫学术研讨会

作者： Luan Fang~(1,2),Yue Xuetian~1,Liu Peng~1,Liu Jun~3,Liang Xiaohong~1,Shi Wei~1,Gao Lifen~1,Spear Brett~4,Ma Chunhong~1 1.Key Laboratory for Experimental Teratology of Ministry of Education and Institute of immunology,shandong university School of Medicine,44 Wenhua Xi Road,jinan,shandong,250012 P.R.china. 2.department of Clinical laboratory,provincial hospital affiliated to shandong university,324 Jingwu Road,jinan,shandong,250021 P.R.china. 3.department of Surgery,provincial hospital affiliated to shandong university,324 Jingwu Road,jinan,shandong,250021 P.R.china. 4.department of Microbiology,immunology,&Molecular Genetics and Markey Cancer Center,university of Kentucky College of Medicine,Lexington,KY,USA.

Background & Aims Glypican 3(GPC3) expresses abundantly in fetal liver and is silent in normal adult liver but becomes reactivated in hepatocellular carcinoma(HCC).And recently GPC3 has been the focus of particular interests because of its potential usefulness as a specific marker for *** study the mechanisms regulating the reactivation of GPC3 in HCC appears to be clinically ***-fingers and homeoboxes 2(ZHX2) are ubiquitously expressed and localized to the nuclei of cells,and appear to function as transcriptional repressors. Previous data have shown that ZHX2 protein is involved in postnatal repression of GPC3 ***,we hypothesized ZHX2 might regulate GPC3 expression in HCC. Methods ZHX2 and GPC3 expression were analyzed by RT-PCR,Western blot and immunohistochemistry to evaluate the correlation of ZHX2 and GPC3 *** effects of ZHX2 overexpressed and sh-RNA mediated ZHX2 knockdown in different HCC cell lines were examined in regard to GPC3 *** assay of ZHX2 in HCC and cytoplasmic/nuclear ZHX2 staining in HCC and adjacent non-tumor sections were carried out to reveal the mechanism of GPC3 reactivation in *** reporter assays and chromatin immunoprecipitation were used to measure effects of ZHX2 on GPC3 promoter. RGSUltS Inverse correlation of ZHX2 and GPC3 expression was demonstrated not only in cultured liver cell lines but also in HCC *** of ZHX2 inHepG2,Hep3B and HepG2.2.15 cells,which express lower endogenous ZHX2,significantly decreases GPC3 *** contrast,ZHX2 knockdown increases GPC3 levels in SMMC7721 and *** these results represent the first direct evidence that ZHX2 represses GPC3 expression in *** assay of ZHX2 inHCC and cytoplasmic/nuclear ZHX2 staining in HCC and adjacent non-tumor sections reveal that decreased nuclear ZHX2 levels in HCC tissues is responsible for GPC3 reactivation. Co-transfections of ZHX2 and GPC3-luciferase reporter genes demon

关键词： ZHX2 GPC3 HCC Negative regulation

来源： cnki会议评论

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Engineering 2023年第11期30卷 190-202页

作者： Fengqing Fu Meng Zheng shandong Zhao Yan Wang Minzhou Huang Hanqing Chen Ziyi Huang Kaijie Zhang Liyan Miao Xueguang Zhang Jiangsu Institute of Clinical immunology the First Affiliated Hospital of Soochow UniversitySuzhou 215000China State Key Laboratory of Radiation Medicine and Protection Soochow UniversitySuzhou 215123China department of Clinical Pharmacology the First Affiliated Hospital of Soochow UniversitySuzhou 215006China Suzhou Bright Scistar Antibody Biotech.Co. Ltd.Suzhou 215152China Institute for Interdisciplinary Drug Research and Translational Sciences College of Pharmaceutical SciencesSoochow UniversitySuzhou 215123China department of Hematology the First Affiliated Hospital of Soochow UniversitySuzhou 215000China

B7 homolog 3(B7-H3)has attracted much attention in glioblastoma(GBM)radioimmunotherapy(RIT)due to its abnormally high expression on tumor *** this study,we report that two specific humanized anti-human B7-H3 antibodies(hu4G4 and hu4H12)derived from mouse anti-human B7-H3 antibodies that were generated by computer-aided design and exclusively recognize membrane expression of B7-H3 by human glioma cells,Hu4G4 and hu4H12 were radiolabeled with^(89)Zr for RIT antibody ***-positron emission tomography(PET)imaging,biodistribution and pharmacokinetic(PK)analyses of^(89)Zr-labeled antibodies were performed in U87-xenografted models.^(125)I labelling of the antibodies for single-photon emission computed tomography(SPECT)imaging was also used to investigate the biological behavior of the antibodies in *** rthermore,the pharmacodynamic(PD)of the^(131)Ilabeled antibodies were evaluated in U87-xenografted mice and GL261 Red-FLuc-B7-H3 in situ glioma tumor ***-PET imaging and biodistribution analysis with a gamma counter showed that^(89)Zr-deferoxamine(DFO)-hu4G4 had higher tumor targeting performance with lower liver uptake than^(89)Zr-DFO-(hu4H12,immunoglobulin G(IgG)).The biodistribution results of^(125)I-SPECT imaging were similar to those of^(89)Zr-PET imaging,though the biodistribution in long bone joints and the thyroid *** PD analysis results indicated that^(131)I-hu4G4 had an excellent therapeutic effect and high safety with no apparent ***,^(131)I-hu4G4 improved the tumor vasculature in tissues with higher expression of collagen typeⅣand platelet-derived growth factor receptorβ(PDGFR-β)compared with control treatment,as determined by immunofluorescence(IF),which contributed to inhibiting tumor *** together,our data indicate that hu4G4 exhibits good tumor targeting and specificity,achieves low nonspecific concentrations in normal tissues,and has acceptable PK characteristics.^(131)I-hu4G4 also exerts effect

关键词： B7-H3 Radioimmunotherapy Glioblastoma Pharmacokinetics Pharmacodynamics

抗风湿药物胃肠道损伤管理中国专家共识(2024版)

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中华风湿病学杂志 2024年第5期28卷 293-302页

作者：黄红段利华姜林娣谈文峰薛静程永静李景南吕宾吴开春张卓莉赵岩中华医学会风湿病学分会中国初级卫生保健基金会风湿免疫学专委会北京大学第一医院风湿免疫科北京100034 江西省人民医院风湿免疫科南昌330006 复旦大学附属中山医院风湿免疫科上海200032 江苏省人民医院风湿免疫科南京210029 浙江大学医学院附属第二医院风湿免疫科杭州310003 北京医院风湿免疫科北京100005 北京协和医院消化科北京100730 浙江中医药大学附属第一医院消化科杭州310006 西京医院消化科西安710032 北京协和医院风湿免疫科北京100730 不详

风湿免疫病患者在接受抗风湿药物治疗过程中可能出现一系列不良反应,其中胃肠道损伤较为常见。由抗风湿药物引起的胃肠道损伤不仅危害患者健康,而且显著增加了医疗费用。为了使临床医生科学、规范地管理抗风湿药物相关的胃肠道损伤,本... 详细信息

风湿免疫病患者在接受抗风湿药物治疗过程中可能出现一系列不良反应,其中胃肠道损伤较为常见。由抗风湿药物引起的胃肠道损伤不仅危害患者健康,而且显著增加了医疗费用。为了使临床医生科学、规范地管理抗风湿药物相关的胃肠道损伤,本共识团队通过对常用抗风湿药物相关胃肠道不良事件及防治方法进行文献检索、文献质量评价,基于循证医学证据制定了《抗风湿药物胃肠道损伤管理中国专家共识》。该共识包括总则的3条陈述和细则的12条推荐意见,提出了风湿免疫病对胃肠道的影响及其表现,抗风湿药物引起胃肠道损伤的机制,风湿免疫病患者服用抗风湿药物相关胃肠道损伤的危险因素,以及抗风湿药物相关胃肠道损伤的防治方法,用于指导临床实践。

关键词：风湿免疫病抗风湿药物胃肠道损伤干预措施共识

Chinese Expert Consensus on the Diagnosis and Treatment of Adult Dermatomyositis (2022)

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International Journal of Dermatology and Venereology 2024年第3期7卷 163-173页

作者： Hua Cao Ai-Jun Chen Yong Cui Dan-Qi Deng Xing-Hua Gao Yan-Ling He Xiao-Jing Kang Hong-Zhong Jin Cheng-Xin Li Feng Li Heng-Jin Li Wen-Jun Liao Xiao-Ming Liu Qian-Jin Lu Yan Lu Meng Pan Wei-Hua Pan Xiao-Ming Shu Qing Sun Ke-Yun Tang Juan Tao Yu Wang Ting Xiao Fu-Ren Zhang Han-Lin Zhang Dermatology Branch of the china.International Exchange and Promotion Association for Medical and Health Care National Clinical Research Center for Dermatologic and Immunologic Diseases department of Dermatology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China department of Dermatology First Affiliated Hospital of Chongqing Medical UniversityChongqing 400042China department of Dermatology China-Japan Friendship HospitalBeijing 100029China department of Dermatology the Second Affiliated Hospital of Kunming Medical UniversityKunmingYunnan 650101China department of Dermatology the First Hospital of China Medical UniversityShenyangLiaoning 110001China department of Dermatology Beijing Chaoyang Hospital Affiliated to Capital Medical UniversityBeijing 100020China department of Dermatology and Venereology People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqiXinjiang Uygur Autonomous Region 830001China department of Dermatology State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100730China department of Dermatology First Medical CenterPLA General HospitalBeijing 100853China department of Dermatology Hainan Hospital of PLA General HospitalSanyaHainan 572013China department of Dermatology Xijing HospitalAir Force Medical UniversityXi’anShaanxi 710032China department of Dermatology Huazhong University of Science and Technology Union Shenzhen HospitalShenzhenGuangdong 518052China hospital for Skin Diseases(Institute of Dermatology) Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China department of Dermatology Jiangsu Province Hospitalthe First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China department of Dermatology Shanghai Changzheng HospitalSecond Affiliated Hospital of Naval Medical UniversityShanghai 200003China department of rheumatology China-Japan Friendship HospitalBeijing 100029China department of Dermatology Qilu Hospital of Shandong UniversityJinanShandong 250012China department of Dermatology Union Hospital

Dermatomyositis,an idiopathic inflammatory myopathy,is characterized by distinctive skin manifestations,proximal muscle weakness,and multiple organ involvement and can be accompanied by *** provide a reference for dermatologists and clinicians in other relevant fields of clinical practice,experts from the Dermatology Branch of the china.International Exchange and Promotion Association for Medical and Health Care and the National Clinical Research Center for Dermatologic and Immunologic Diseases developed this consensus on the diagnosis and treatment of adult dermatomyositis using Chinese and international literature and expert advice.

关键词： dermatomyositis adult diagnosis treatment clinical protocols expert consensus

脂肪来源间充质干细胞移植干预系统性红斑狼疮模型小鼠的免疫功能

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中国组织工程研究 2019年第17期23卷 2696-2702页

作者：叶玲朱静何成松西南医科大学附属医院风湿免疫科四川省泸州市646000 四川省医学科学院.四川省人民医院风湿免疫科四川省成都市610000

背景:虽然间充质干细胞治疗系统性红斑狼疮已取得临床疗效,但其免疫调节的内源性功能及作用机制有待深入研究。目的:探讨来源的脂肪间充质干细胞对系统性红斑狼疮小鼠(MRL/lpr小鼠)免疫功能的影响及其可能机制。方法:将36只MRL/lpr小鼠... 详细信息

背景:虽然间充质干细胞治疗系统性红斑狼疮已取得临床疗效,但其免疫调节的内源性功能及作用机制有待深入研究。目的:探讨来源的脂肪间充质干细胞对系统性红斑狼疮小鼠(MRL/lpr小鼠)免疫功能的影响及其可能机制。方法:将36只MRL/lpr小鼠随机分为3组:模型对照组不进行干预、磷酸盐缓冲液对照组尾静脉注射磷酸盐缓冲液、干细胞移植组尾静脉注射脂肪间充质干细胞;另取12只C57BL/6小鼠作为正常对照组。结果与结论:①分离MRL/lpr小鼠脂肪组织获得的细胞具有脂肪间充质干细胞特性;②模型对照组、磷酸盐缓冲液对照组和干细胞移植组的血清尿素氮、肌酐和抗双链DNA抗体水平均高于正常对照组(P 2比例高于正常对照组(P 2比值低于正常对照组和干细胞移植组(P 2、干扰素γ、白细胞介素10和T淋巴细胞产生的淋巴毒素水平降低,白细胞介素4水平升高(P 2、白细胞介素10、干扰素γ和T淋巴细胞产生的淋巴毒素水平升高,白细胞介素4水平降低(P <0.05)。结果表明脂肪间充质干细胞移植可通过提高树突状细胞和调节性T细胞比例、促进辅助性T细胞亚型的转化对系统性红斑狼疮小鼠免疫功能产生影响。

关键词：脂肪间充质干细胞系统性红斑狼疮干细胞移植尾静脉注射组织工程小鼠脾脏调节性T细胞树突状细胞辅助性T细胞

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痛风诊疗规范

中华内科杂志 2023年第9期62卷 1068-1076页

作者：徐东朱小霞邹和建林禾赵岩代表中华医学会风湿病学分会中国医学科学院、北京协和医学院、北京协和医院风湿免疫科、国家皮肤与免疫疾病临床医学研究中心、疑难重症及罕见病国家重点实验室、风湿免疫病学教育部重点实验室北京100730 复旦大学附属华山医院风湿免疫科上海200040 福建省立医院风湿免疫科福州350013 不详

痛风是一种单钠尿酸盐沉积在关节所致的晶体相关性关节病,属代谢性风湿病。我国痛风虽然并不少见,但其规范化诊疗的普及依然欠缺。中华医学会风湿病学分会查阅了该领域最新进展,在借鉴国内外诊治经验的基础上,制定了本规范,旨在规范痛... 详细信息

痛风是一种单钠尿酸盐沉积在关节所致的晶体相关性关节病,属代谢性风湿病。我国痛风虽然并不少见,但其规范化诊疗的普及依然欠缺。中华医学会风湿病学分会查阅了该领域最新进展,在借鉴国内外诊治经验的基础上,制定了本规范,旨在规范痛风的诊断、治疗时机和治疗方案,以减少误诊和漏诊。对患者短期与长期治疗予以建议,以减少不可逆损伤的发生,改善患者预后。

关键词：痛风高尿酸血症诊断治疗预后

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原发性干燥综合征诊疗规范

中华内科杂志 2023年第9期62卷 1059-1067页

作者：张文陈竹厉小梅高洁赵岩代表中华医学会风湿病学分会中国医学科学院、北京协和医学院、北京协和医院风湿免疫科、国家皮肤与免疫疾病临床医学研究中心、疑难重症和罕见病国家重点实验室、风湿免疫病教育部重点实验室北京100730 中国科学技术大学附属第一医院安徽省立医院风湿免疫科合肥230001 海军军医大学第一附属医院风湿免疫科上海200433 不详

干燥综合征(SS)是一种以淋巴细胞增殖及进行性外分泌腺体损伤为特征的慢性炎症性自身免疫病。临床除有唾液腺、泪腺功能受损外,亦可出现多系统多脏器受累,血清中存在自身抗体和高免疫球蛋白血症。SS根据是否伴发其他结缔组织病分为继发... 详细信息

干燥综合征(SS)是一种以淋巴细胞增殖及进行性外分泌腺体损伤为特征的慢性炎症性自身免疫病。临床除有唾液腺、泪腺功能受损外,亦可出现多系统多脏器受累,血清中存在自身抗体和高免疫球蛋白血症。SS根据是否伴发其他结缔组织病分为继发性SS和原发性SS(pSS),前者常继发于系统性红斑狼疮、类风湿关节炎等。中华医学会风湿病学分会在借鉴国内外诊治经验和指南的基础上,制订了本规范,旨在规范SS诊断中关键指标(如血清抗SSA抗体和唇腺病理学)的检测和解读,采用公认的疾病活动指标评价疾病,规范局部和系统受累的合理诊治。

关键词：干燥综合征诊断治疗预后

未分化结缔组织病和混合性结缔组织病的诊疗规范

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中华内科杂志 2022年第10期61卷 1119-1127页

作者：莫颖倩严青叶霜戴冽赵岩中华医学会风湿病学分会中山大学孙逸仙纪念医院风湿免疫科广州510120 福建省立医院风湿免疫科福州350001 上海交通大学医学院附属仁济医院风湿免疫科上海200001 中国医学科学院北京协和医学院北京协和医院风湿免疫科国家皮肤与免疫疾病临床医学研究中心疑难重症和罕见病国家重点实验室风湿免疫病学教育部重点实验室北京100730

未分化结缔组织病(CTD)通常指患者出现某些CTD相关的症状和体征,同时伴自身免疫病的血清学证据,但不符合任意一确定的CTD分类标准。混合性CTD是指患者出现手肿胀、滑膜炎、肌炎、雷诺现象、肢端硬化等一种或多种临床表现,伴血清高滴度... 详细信息

未分化结缔组织病(CTD)通常指患者出现某些CTD相关的症状和体征,同时伴自身免疫病的血清学证据,但不符合任意一确定的CTD分类标准。混合性CTD是指患者出现手肿胀、滑膜炎、肌炎、雷诺现象、肢端硬化等一种或多种临床表现,伴血清高滴度斑点型抗核抗体(ANA)和高滴度抗U1核糖核蛋白(U1RNP)抗体阳性,而抗Sm抗体阴性。未分化CTD和混合性CTD的规范化诊断和治疗的更新慢于其他确定的CTD,治疗均缺乏随机对照研究证据或指南推荐,目前主要按照构成疾病的具体临床表现而采用相应的常规治疗措施。中华医学会风湿病学分会在既往诊治指南及近年研究进展的基础上制定了本规范,旨在提高国内未分化CTD和混合性CTD的临床诊治水平,改善患者预后。

关键词：未分化结缔组织病混合性结缔组织病诊断治疗预后

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结缔组织病相关肺动脉高压诊疗规范

中华内科杂志 2022年第11期61卷 1206-1216页

作者：张晓赵久良丁峰杨静王静曾小峰赵岩中华医学会风湿病学分会广东省医学科学院广东省人民医院风湿免疫科广州510120 中国医学科学院北京协和医学院北京协和医院风湿免疫科国家皮肤与免疫疾病临床医学研究中心疑难重症及罕见病国家重点实验室风湿免疫病学教育部重点实验室北京100730 山东大学齐鲁医院风湿免疫科济南250012 绵阳市中心医院风湿免疫科绵阳621000 云南省第一人民医院昆明理工大学附属医院风湿免疫科昆明650032 不详

肺动脉高压(PAH)是多种原因导致肺动脉压升高而伴发的一系列临床病理综合征,PAH亦是结缔组织病(CTD)最严重的并发症。近年来有关PAH的诊治及评估有了很大进步,为提高风湿科医生对CTD相关PAH的认识与诊治水平,中华医学会风湿病学分会在... 详细信息

肺动脉高压(PAH)是多种原因导致肺动脉压升高而伴发的一系列临床病理综合征,PAH亦是结缔组织病(CTD)最严重的并发症。近年来有关PAH的诊治及评估有了很大进步,为提高风湿科医生对CTD相关PAH的认识与诊治水平,中华医学会风湿病学分会在目前国内外经验和指南的基础上,制定了本规范,旨在指导CTD相关PAH早期筛查、早期诊断和早期干预,以及如何进行患者随访与管理,以改善CTD相关PAH患者的长期预后。

关键词：高血压肺性结缔组织疾病诊断治疗预后