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Identification of AtENT3 as the main transporter for uridine uptake in Arabidopsis roots

cell Research 2006年第4期16卷 377-388页

作者： Kun Ling Chen~(1,2,*) Min Xin Xu~(2,3,*) Guang Yong Li~(2,3,*) Hui Liang~2 Zong Liang Xia~(2,3) Xin Liu~2 Ji Shu Zhang~1 Ai Min Zhang~2 Dao Wen Wang~2 ~1School of Life sciences,Northwest Agricultural and Forestry University,Yangling 712100,china ~2center for plant gene Research(beijing)and the state kev laboratory of plant cell and chromosome engineering,{13.,Chinese academy of sciences,datun road,chaovang district,beijing 1.01.1.china ~3Graduate School of the Chinese academy of sciences,Yuquan road,Shijingshan district,beijing 100039,china School of Life sciences Northwest Agricultural and Forestry University Yangling China center for plant gene Research (beijing) and the state Key laboratory of plant cell and chromosome engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences Datun Road Chaoyang District Beijing China Graduate School of the Chinese academy of sciences Yuquan Road Shijingshan District Beijing China

Previous studies have shown that Arabidopsis equilibrative nucleoside transporters （AtENTs） possess transport activitieswhen produced in yeast cells and are differentially expressed in Arabidopsis ***,we report further analysis onthe nucleoside transport activities and transcriptional patterns of AtENT *** recombinant proteins of AtENTs3,6,and 7,but not those of AtENTs 1,2,4,and 8,were found to transport thymidine with high *** to previ-ous suggestion that AtENT1 may not transport uridine,this work showed that recombinant AtENT1 was a pH-dependentand high-affinity transporter of *** grown on MS plates,the AtENT3 knockout plants were more tolerant tothe cytotoxic uridine analog 5-fluorouridine than wild-type plants and the knockout plants ofAtENT 1 or ***-sistent with this observation,the AtENT3 knockout line exhibited a significantly decreased ability to take up [3H]uridinevia the roots when compared with wild-type plants and the plants with mutated AtENT 1 or *** indicates thatAtENT3,but not AtENTs 1 and 6,is the main transporter for uridine uptake in Arabidopsis *** transcription ofAtENTs 1,3,4,6,7,and 8 was regulated in a complex manner during leaf development and *** contrast,thesix AtENT members were coordinately induced during seed *** work provides new information on thetransport properties of recombinant AtENT proteins and new clues for future studies of the in vivo transport activitiesand physiological functions of the different ENT proteins in Arabidopsis plants.

关键词： Arabidopsis equilibrative nucleoside transporter expression pattern nucleoside transport functional analysis

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