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Role of arachidonic acid in hyposmotic membrane stretch-induced increase in calcium-activated potassium currents in gastric myocytes

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Acta Pharmacologica Sinica 2005年第10期26卷 1233-1242页

作者： Meng YANG~2, Xing-lan LI~3, Hui-ying XU~3, Jia-bin SUN~3, Bin MEI~4, Hai-feng ZHENG~3, Lian-hua PIAO~3, De-gang XING~3 Zhai-liu LI~3, Wen-xie XU~(2,5) ~2 department of Physiology, shanghai Jiaotong university School of Medicine, shanghai 200030 ~3 department of Physiology Yanbian university college of Medicine Yanji 133000 ~4 East china Normal university School of Life Science, shanghai 200062, china department of Physiology Shanghai Jiaotong University School of Medicine Shanghai China department of Physiology Yanbian University College of Medicine Yanji China East china Normal university School of Life Science Shanghai China

Aim: To study effects of arachidonic acid （AA） and its metabolites on the hyposmotic membrane stretch-induced increase in calcium-activated potassium currents (IKCa) in gastric myocytes. Methods: Membrane currents were recorded by using a conventional whole cell patch-clamp technique in gastric myocytes isolated with collagenase. Results: Hyposmotic membrane stretch and AA increased both IK（Ca）and spontaneous transient outward currents significantly. Exogenous AA could potentiate the hyposmotic membrane stretch-induced increase in IK（Ca）The hyposmotic membrane stretch-induced increase inK（Ca）was significantly suppressed by dimethyleicosadienoic acid （100 μmol/L in pipette solution）, an inhibitor of phospholipase A2. Nordihydroguaiaretic acid, a lipoxygenase inhibitor, significantly suppressed AA and hyposmotic membrane stretch-induced increases in IK（Ca）. External calcium-free or gadolinium chloride, a blocker of stretch-activated channels, blocked the AA-induced increase in IK（Ca）significantly, but it was not blocked by nicardipine, an L-type calcium channel blocker. Ryanodine, a calcium-induced calcium release agonist, completely blocked the AA-induced increase inK（Ca）; however, heparin, a potent inhibitor of inositol triphosphate receptor, did not block the AA-induced increase in IK（Ca）. Conclusion: Hyposmotic membrane stretch may activate phospholipase A2, which hydrolyzes membrane phospholipids to ultimately produce AA; AA as a second messenger mediates Ca2+influx, which triggers Ca2+-induced Ca2+release and elicits activation of IK（Ca）in gastric antral circular myocytes of the guinea pig.

关键词： gastric myocyte arachidonic acid hyposmotic membrane stretch calcium-activated potassium current.

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Inhibitory effects of idoxifene on hepatic fibrosis in rats

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Acta Pharmacologica Sinica 2005年第5期26卷 581-586页

作者： Ya-jun ZHOU~2,Dong-mei YIN,Hong-shan CHEN,Jian-hua SHI,Bao-xi SHA,Xing WANGdepartment of biochemistry & Molecular Biology medical college,Nan Tong university,Nantong 226001,china department of biochemistry & Molecular Biology Medical College Nan Tong University Nantong China

Aim:To investigate the effects of a tissue-specific selective estrogen receptormodulator,idoxifene,on hepatic fibrosis in ***:Hepatic fibrosis wasinduced by dimethylnitrosamine(DMN)in male *** DMN model of hepaticfibrosis and the hepatocytes undergoing oxidative stress were treated withidoxifene *** effect of idoxifene on hepatic fibrosis in the DMNmodel was examined by *** of idoxifene on antioxi-dant enzyme levels of copper,zinc-dependent superoxide dismutase(CuZn-SOD),and cellular glutathione peroxidase(GSHPx)were measured by *** ofidoxifene on activation,proliferation,and apoptosis of culture-activated hepaticstellate cells(HSC)were analysed by immunohistochemistry,bromodeoxyuridine(BrdU)uptake,and flow cytometry, respectively. Results:Idoxifene could mark-edly suppress DMN-induced hepatic fibrosis in male rats.A treatment of 0.4mg·kg-1·d-1of idoxifene reduced the protein levels of collagen in the DMN modelby 41.19%(P2.65 times(P2.08 times greater(P2.52 times(P2%(P<0.05).Conclusion:Idoxifene showed inhibi-tory action on hepatic fibrosis in male rats.

关键词： fibrosis idoxifene oxidative stress hepatic stellate cell liver

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Amphiphilic dendronized homopolymers

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Science china Chemistry 2010年第12期53卷 2509-2519页

作者： LI Wen 1 & ZHANG AFang 1,2 1 Institute of Polymers,department of Materials,ETH Zurich,Wolfgang-Pauli-Str.10,8093 Zurich,Switzerland 2 department of Polymer Materials,college of Materials Science and Engineering,shanghai university,shanghai 201800,china Institute of Polymers Department of Materials ETH Zurich Zurich Switzerland department of Polymer Materials College of Materials Science and Engineering Shanghai University Shanghai China

A series of second generation of amphiphilic dendronized homopolymers are efficiently synthesized,and their thermoresponsiveness in aqueous solutions and secondary structures in methanol solutions are *** polymers are constructed in each repeat unit with various generations of hydrophobic 4-aminoproline and hydrophilic oligoethylene glycol (OEG)-based dendrons,and their over-all hydrophilicity is tuned by varying these dendron *** with or without the first generation of proline dendron show good water solubility at room temperature,but exhibit typical thermoresponsive behaviors at elevated temperatures as characterized by turbidity measurements using UV-vis spectroscopy,while the polymer with the secondary generation of proline dendron is not soluble in *** polymers show ordered secondary structures as evidenced by the optical rotation and circular dichroism ***,assembly of these amphiphilic homopolymers into porous films via breath figure (BF) technique is described,and polymer structures are found to show significant influence on the morphology of porous film.

关键词： amphiphilic polymer dendronized polymer dendritic macromolecule supramolecular structure

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Synergistic extraction of praseodymium with 2-ethylhexyl phosphonic acid mono-2-ethylhexyl ester and 8-Hydroxyquinoline

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Journal of shanghai university(English Edition) 2009年第1期13卷 72-75页

作者：张倩乌东北包伯荣 department of Chemistry College of SciencesShanghai University department of Chemistry Tongji University

The synergistic extraction of Pr^3＋ from hydrochloric medium using mixture of 2-ethylhexyl phosphonic acid mono- 2-ethylhexyl ester （P507, HL） and 8-Hydroxyquinoline （HQ） in heptane was investigated. The effect of equilibrium of aqueous acidity on extraction of Pr^3＋ was discussed. The effect of extractant concentraction, different diluents, equilibrium time and acetate ion concentration oil extraction reaction were also studied. With a method of double-logarithmic slope, composition of the extracted species on 2-ethylhexyl phosphonic acid mono-2-ethylhexyl ester and 8-Hydroxyquinoline was derived. The result shows that the synergistic extraction system not only overcomes emulsification of 8-Hydroxyquinoline, but also shows perfect capacity of synergistic extraction. The largest synergistic enhancement factor can be calculated to be 5.49 at pH 3.6 for Pr^3＋.

关键词： 2-ethylhexyl phosphonic acid mono-2-ethylhexyl ester 8-Hydroxyquinoline (HQ) synergistic extraction,praseodymium

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A Novel Gene Delivery System Targeting Urokinase Receptor

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 485-491页

作者： Xing-Hui SUN, Li TAN, Chun-Yang LI, Chang TONG, Jin FAN, Ping LI, and Yun-Song ZHU*department of Molecular Genetics, Key Laboratory of Molecular Medicine of Ministry Education,shanghai medical college of Fudan university, shanghai 200032, china department of Molecular Genetics Key Laboratory of Molecular Medicine of Ministry Education Shanghai Medical College of Fudan UniversityShanghai 200032 China

Recombinant proteins that combine different functions required for cell targeting and intra- cellular delivery of DNA present an attractive approach for the development of nonviral gene delivery vectors. Here, we described a novel protein termed ATF-lys10 which facilitated cell-specific gene transfer via receptor-mediated endocytosis. ATF-lys10 was composed of the amino-terminal fragment of urokinase and ten lysines at the carboxyl terminus. Bacterially expressed ATF-lys10 protein existed in soluble form, and had antigenicity of human urokinase. Purified ATF-lys10 specifically bound to uPAR-expressing cells and formed protein-DNA complexes with plasmid pGL3-control. After neutralization of excess negative charge with poly-L-lysine, these complexes served as a specific gene delivery vector for uPAR-expressing cells. Lyso- somotropic compounds, such as chloroquine, drastically increased the ATF-lys10 mediated gene delivery efficiency. Our results suggest that the recombinant protein ATF-lys10 with the properties of DNA binding and tumor cell targeting represents a promising method for gene transfer and expression in tumor cells.

关键词： amino-terminal fragment urokinase gene transfer endocytosis urokinase receptor

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Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line

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Acta Pharmacologica Sinica 2003年第12期24卷 61-66页

作者： WANG Lei, ZHANG Gui-Mei, FENG Zuo-Hua department of biochemistry and Molecular Biology, tongji medical college Huazhong university of Science and Technology, Wuhan 430030, china department of biochemistry and Molecular Biology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

AIM: To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in downregulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line. METHODS: The expression of survivin mRNA was measured by RTPCR and the expression of survivin protein was measured by Western blot. Caspase-3 activity was determined by Phar Mingen colorimetric assay kit. Apoptosis was assessed by flow cytometry. The chemosensitivity of HL-60/ADR cells to adriamycin (ADR) was measured by MTT assay. RESULTS: pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, and induced apoptosis of HL-60/ADR cells in a time-dependent manner during 12-48 h. After transient transfection with pcDNA3.1/survivin for 48 h, survivin mRNA decreased by 67％, survivin protein decreased by 57％, caspase-3 activity increased 4.37 times, and the apoptosis rate increased by 4.41％ compared wi

关键词： antisense RNA doxorubicin apoptosis survivin caspase-3 HL-60 cells multiple drug resistance

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Detection of germline mutations of hMLH1 and hMSH2 based on cDNA sequencing in china

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World Journal of Gastroenterology 2005年第42期11卷 6620-6623页

作者： Chao-Fu Wang, Xiao-Yan Zhou, Tai-Ming Zhang, Meng-Hong Sun, Da-Ren Shi, Laboratory of Molecular Pathology, Cancer Hospital of Fudan university department of Oncology, shanghai medical college of Fudan university, shanghai 200032, china Laboratory of Molecular Pathology Cancer Hospital of Fudan University Shanghai 20032 China.

AIM： TO detect the germline mutations Of hMLH1 and hMSH2 based on mRNA sequencing to identify hereditary non polyposis oolorectal cancer （HNPCC） families. METHODS： Total RNA was extracted from peripberal blood of 14 members from 12 different families fulfilling Amsterdam criteria II. mRNA of hMLH1 and hMSH2 was reversed with special primers and heat-resistant reverse tmnscriptase, cDNA was amplified with expand long template PCR and cDNA sequendng analysis was followed. RESULT： Seven germline mutations were found in 6 families （6/12, 50%）, in 4 hMLH1 and 3 hMSH2 mutations （4/12, 33.3%）; （3/12, 25%）. The mutation types involved 4 missense, 1 silent and 1 frame shift mutations as well as 1 mutation in the non-coding area. Four out of the seven mutations have not been reported previously. The 4 hMLH1 mutations were distributed in exons 8, 12, 16, and 19. The 3 hMSH2 mutations were distributed in exons 1 and 2. Six out of the 7 mutations were pathological, which were dislTibuted in 5 HNPCC families. CONCLUSION： Germline mutations of hMLH1 and hMSH2 can be found based on cDNA sequencing so as to identify HNPCC family, which is highly sensitive and has the advantages of cost and time saving.

关键词： hMLH1 hMSH2 Colorectal cancer Hereditarynon-polyposis Reverse transcription Germline mutation

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A two-dimensional study on natural convection and heat transfer in the enclosure with heat transfer and radiation coupled in natural convection

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Science china(Technological Sciences) 2010年第4期53卷 991-999页

作者： ZHAN NaiYan1,2,YANG Mo1 & XU PeiWei2 1 college of Power Engineering,university of shanghai for Science and Technology,Shanghiai 200093,china 2 department of Environmental Engineering,Jilin Architectural and Civil Engineering Institute,Changchun 130021,china college of Power Engineering University of Shanghai for Science and Technology Shanghiai China department of Environmental Engineering Jilin Architectural and Civil Engineering Institute Changchun China

Numerical investigation using SIMPLE algorithm with QUICK scheme for natural convection and heat transfer in the enclosure bounded by a solid wall and with heat transfer and radiation coupled in natural convection has been *** various parameters are:Rayleigh number(from 103 to 105),dimensionless conductivity of bounding wall(from 0 to 100),dimensionless wall thickness(from 0 to 0.6) and radiation emissivity of all surfaces(from 0 to 1).The results suggest that flow and heat transfer are influenced by *** is a dominant action on flow and heat *** increase of the thermal conductivity of wall,flow and heat transfer turn *** temperature distribution changes *** the thermal conductivity of wall is over a certain critical number,the increasing trend of flow and heat transfer may *** increase of enclosure wall thickness,flow and heat transfer turn *** the enclosure wall thickness is over a certain critical number,the flow and heat transfer will turn slow.

关键词： conduction natural convection radiation couple numerical simulation

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pH-Dependent Stability of EGX, A Multi-functional Cellulase from Mollusca, Ampullaria crossean

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 603-608页

作者： Wen-Ying LI2, Ji WANG1, Yan-Hong LI1, Ming DING1, Gen-Jun XU1,3, Lan-Ying LIU2, and Fu-Kun ZHAO1,3* 1Key Laboratory of Proteomics, Institute of biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences, shanghai 200031, china 2college of Life Science, Jilin university, Changchun 130023, china college of Life Science, Zhejiang university of Science and Technology, Hangzhou 310018, china college of Life Science Jilin UniversityChangchun 130023 China Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China college of Life Science Zhejiang University of Science and TechnologyHangzhou 310018 China

The cellulase activity and stability of EGX, a multi-functional cellulase previously purified from the mollusca Ampullaria crossean, was systematically studied under different pH. The pH induced con- formation and stability change of EGX have been investigated by using the intrinsic fluorescence, ANS fluorescence and CD spectrum. It has been found that the conformation and activity of this cellulase were strongly dependent on the pH. EGX was stable for both the enzyme activity and the conformation from pH 5.6 to pH 7.4. As shown by intrinsic and ANS fluorescence, no red shift of emission maximum occurred and a negligible intensity change was observed at pH 5.6–7.4. The activity of EGX remained about 80% in pH 5.6–7.4 and obviously decreased out of side the pH range. Urea-induced changes in EGX at pH 5.4 and pH 8.0 were measured by intrinsic fluorescence and CD spectrum. At pH 5.4, a significantly red shift of emission maximum occurred when the concentration of urea was 5 M compared to the concentration was 3 M at pH 8.0. The α-helix at pH 5.4 was 40.51% in the absence of urea and 31.04% in the presence of 4 M urea. At pH 8.0 the α-helix was 7.23% in the presence of 4 M urea. The data indicated that EGX was much susceptible to urea-induced unfolding at pH 8.0 and much stable at pH 5.4. The greater pH dependent stability of EGX may allow the enzyme to adequately catalyze the hydrolysis of cellulosic materials under natural or industrial extreme conditions.

关键词： multi-functional cellulase stability Ampullaria crossean fluorescence spectroscopy pH-dependent structural change CD spectrum

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Resting state brain activity and functional brain mapping

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Progress in Natural Science:Materials International 2007年第5期17卷 511-519页

作者： Zhao Xiaohu , Wang Peijun and Tang Xiaowei (college of life Sciences in Zhejiang university, Hangzhou 310058, china Imaging department of Tong Ji Hospital of Tong Ji university, shanghai 200065, china Bio-X Lab, department of Physics, Zhejiang university, Hangzhou 310027, china) college of Life Sciences Zhejiang University Hangzhou 310058 China Imaging department Tongji Hospital Tongji University Shanghai 200065 China Bio-X Lab. Department of Physics Zhejiang University Hangzhou 310027 China

Functional brain imaging studies commonly use either resting or passive task states as their control conditions, and typically identify the activation brain region associated with a specific task by subtracting the resting from the active task conditions. Numerous studies now suggest, however, that the resting state may not reflect true mental "rest" conditions. The mental activity that occurs during "rest" might therefore greatly influence the functional neuroimaging observations that are collected through the usual subtracting analysis strategies. Exploring the ongoing mental processes that occur during resting conditions is thus of particular importance for deciphering functional brain mapping results and obtaining a more comprehensive understanding of human brain functions. In this review article, we will mainly focus on the discussion of the current research background of functional brain mapping at resting state and the physiological significance of the available neuroimaging data.

关键词： resting state brain activity default mode brain networks BOLD deactivation OEF.

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