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Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity

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Journal of Integrative Agriculture 2017年第4期16卷 774-788页

作者： WANG zuo-ping DENG Li-hua WENG Lü-shui DENG Xiang-yang fu Xi-qin XIN Ye-yun XIAO Guo-ying Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 410125 P.R.China Graduate University of Chinese Academy of Sciences Beijing 100049 P.R.China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center Changsha 410125 P.R.China

The developing trends of livestock production are efficiency availability of phytate phosphorus and abuse of antibiotics. safety and sustainability, which face two major challenges： low As a solution phytases and antimicrobial peptides are applied as feed additives. However, phytases and antimicrobial peptides are susceptible to proteases, costly by fermentation and potential toxic to production hosts. We transformed an optimized phytase-lactoferricin fusion gene PhyLfdriven by an en- dosperm-specific promoter Gt13aP and Bar （bialaphos resistance） gene as a selection maker into rice. The Bar and PhyLf genes were integrated into the rice genome, stably inherited and expressed. Their phosphinothricin acetyl transferase （PAT） protein content oftransgenic plants with glufosinate resistance varied between 50.45-93.39 IJg g-l. fusion protein expressed especially in the seeds of transgenic rice had a summit phytase activity at 32.30 U g-l, which increased by 61.71-fold com- pared to the control/check group （CK） and 7.54-fold compared to un-optimized transgenic plant. The highest inorganic phosphorus （Pi） content of the transgenic seeds reached 13.15 mg g-~, increased by 12.77-fold compared to that of CK. Preliminary antibacterial experiments showed that the enterokinase hydrolysate product of fusion protein could inhibit the growth of Escherichia coil DH5a. These results indicated that the protein PhyLf has the potential to increase availability of feed phytate phosphorus, improve consumer＇s immunity and reduce the use of antibiotics.

关键词： rice phytase antimicrobial peptide lactoferricin fusion gene Bar gene herbicide resistance

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Molecular characterization and tissue expression profile of the Dnmts gene family in pig

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Journal of Integrative Agriculture 2017年第6期16卷 1367-1374页

作者： LUO Zong-gang ZHANG Kai CHEN Lei YANG Yuan-xin fu Peng-hui WANG Ke-tian WANG Ling LI Ming-zhou LI Xue-wei zuo fu-yuan WANG Jin-yong Chongqing Academy of Animal Science Chongqing 402460 P.R. China Department of Animal Science Southwest University (Rongchang Campus) Chongqing 402460 P.R. China Key Laboratory of Pig Industry Sciences Ministry of Agriculture Chongqing 402460 P.R. China Sichuan Academy of Grassland Sciences Chengdu 610000 P.R.China Institute of Animal Genetics & Breeding College of Animal Science & Technology Sichuan Agricultural University Chengdu 611130 P.R.China

DNA methyltransferases（Dnmts）comprise a family of proteins which involved in the establishment and maintenance of DNA methylation *** pig,the molecular characterization and tissue expression profile of Dnmt gene family are not *** solve this problem,reverse transcriptase PCR and rapid amplification of c DNA ends were used to clone the sequences of the porcine Dnmt2 and Dnmt3b ***,the m RNA expression profiles of Dnmt1,Dnmt2,Dnmt3a and Dnmt3b genes from 54 adult tissues and 2 entire fetuses of Rongchang pig were analyzed by quantitative real-time PCR（q RT-PCR）.As a result,the lengths of porcine Dnmt2 and Dnmt3b gene c DNAs were 1 227 and 2 559 bp with cytosine-C5specific DNA methylase domain,*** four Dnmt genes were highly expressed in longissimus dorsi muscle（P〈0.01）.Dnmt1 is highly expressed in heart（P〈0.01）and Dnmt 2 shows its preference in liver and seminal vesicle tissue（P〈0.01）.Dnmt3a and Dnmt3b are highly expressed in the two fetus stages（P〈0.01）.All these results suggested that each gene has its specific expression profile,and deeper study is required to dig more details between the methylation level and Dnmt family m RNA expressions in different tissues.

关键词： pig Dnmt gene family cloning mRNA expression

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Role of rotational state-selected for nonadiabatic alignment： OCS molecules in femtosecond laser fields

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Frontiers of physics 2013年第2期8卷 236-240页

作者： Rui-Han Zhu Chun-Cheng Wang Si-zuo Luo Xue Yang Mei-Xia Zhang fu-Chun Liu Da-Jun ding Institute of Atomic and Molecular Physics Jilin UniversityChina Changchun 130012 School of Science Changchun University of Science and TechnologyChina Changchun 130022

Nonadiabatic alignment by intense nonresonant the spatial direction of molecules： By solving the the degree of alignment of the molecules initially laser fields is a versatile technique to manipulate time-dependent SchrSdinger equation numerically in different rotational state are calculated and the results show that the degree of alignment strongly depends on the initial rotational state. Thus, the present study indicates that, for obtaining a high degree of alignment for molecules, appropriate selection of molecular rotational states is necessary.

关键词： nonadiabatic alignment rotational state-selection linear triatomic molecule

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Determination the Hydrogen Stored in Carbon Nanotubes by NO Titration Method

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Chinese Chemical Letters 2001年第11期12卷 1015-1018页

作者： Shui Hua TANG Li Zhen GAO Bao Chun LIU He LI Bo Lan ZHANG Liang fu ZHANG zuo Long YU Chengdu Institute of Organic Chemistry Chinese Academy of SciencesChengdu 61004 Institute of Chemical Engineering Materials CAEPMianyang 621900

A novel method was established to determine the H-2-storage capacity in carbon nanotubes. According to 2NO+2H(2) (or 4H) = N-2+2H(2)O reaction, the H-2-storage capacity in carbon nanotubes could be calculated. The H-2... 详细信息

关键词： NO titration hydrogen storage carbon nanotubes

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Clinical efficacy of gemcitabine and cisplatin-based transcatheter arterial chemoembolization combined with radiotherapy in hilar cholangiocarcinoma

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World Journal of Gastrointestinal Oncology 2019年第6期11卷 489-498页

作者： Wen-Heng Zheng Tao Yu Ya-Hong Luo Ying Wang Ye-fu Liu Xiang-Dong Hua Jie Lin zuo-Hong Ma fu-Lu Ai Tian-Lu Wang Department of Medical Imaging Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyang 110042Liaoning ProvinceChina Department of Hepatobiliary and Pancreatic Surgery Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyang 110042Liaoning ProvinceChina Department of General Surgery (VIP ward) Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyang 110042Liaoning ProvinceChina Department of Radiotherapy Cancer Hospital of China Medical UniversityLiaoning Cancer Hospital and InstituteShenyang 110042Liaoning ProvinceChina

BACKGROUND Radical surgical resection is regarded as the best treatment for hepatic hilar cholangiocarcinoma. However, 60%-70% of patients have lost the chance of surgery at the time of diagnosis. Simple biliary stent or drainage tube placement may fail in a short time due to tumor invasion or overgrowth, bile accumulation, or biofilm formation. Effective palliative treatments to extend the effective drainage time are of great significance for improving the quality of life of patients and changing the prognosis of patients. AIM To investigate the clinical efficacy of gemcitabine and cisplatin-based transcatheter arterial chemoembolization (TACE) combined with radiotherapy in hilar *** A retrospective analysis was conducted on patients clinically diagnosed with hilar cholangiocarcinoma from June 2014 to January 2017 at the Liaoning Provincial Cancer Hospital. Patients were evaluated by specialists, and those who were not suitable for surgery or unwilling to undergo surgery and met the inclusion criteria were included in the study. There were a total of 72 patients (34 males and 38 females) with an average age of 59.9 years (range, 40-72 years). According to percutaneous transhepatic biliary angiography and the patients’ wishes, stent implantation or biliary drainage tube implantation was used to relieve biliary obstruction. The patients were divided into either a control group or a combined treatment group according to their follow-up treatment. The control group consisted of a total of 35 patients who received simple biliary drainage tube placement and biliary stent implantation (7 patients with bilateral stents and 6 with a unilateral stent) and 22 patients receiving biliary drainage tube placement alone. The combined treatment group received TACE and extracorporeal radiotherapy after biliary drainage or biliary stent implantation and consisted of a total of 37 patients, including 21 patients receiving combined treatment after biliary stent p

关键词： Hilar cholangiocarcinoma Biliary stent Percutaneous biliary drainage Gemcitabine Cisplatin Radiotherapy Transcatheter arterial chemoembolization

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Design and application of a multichannel"cross"hot tearing tendency device:A study on hot tearing tendency of Al alloys

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China Foundry 2022年第4期19卷 327-334页

作者： Ming Su Wen-tao Zheng Deng-ke fu Hong-jun Huang Xiao-jiao zuo Chun-yu Yue Yu-xiang Wang Xiao-guang Yuan School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China

Hot tearing is one of the most serious defects during the casting solidification *** this study,a new type of multichannel"cross"hot tearing device was *** hot cracks initiation and propagation were predicted by the relationship between temperature,shrinkage force and solidification time during the casting solidification *** reliability and practicability of the multichannel"cross"hot tearing device were verified by casting experiments and numerical *** theoretical calculation based on Clyne-Davies model and numerical simulation results show that the hot tearing tendency decreases in the order:2024 Al alloy>Al-Cu alloy>Al-Si alloy at a pouring temperature of 670°C and a mold temperature of 25°*** of liquid films at the end of solidification plays an important role in the propagation process of hot *** decrease of hot tearing tendency is attributed to the feeding of liquid film and intergranular bridging.

关键词： hot tearing testing device hot cracking tendency solidification process formation mechanism numerical simulation

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Fisetin mitigates hepatic ischemia-reperfusion injury by regulating GSK3β/AMPK/NLRP3 inflammasome pathway

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Hepatobiliary & Pancreatic diseases International 2021年第4期20卷 352-360页

作者： Jun-Liang Pu zuo-Tian Huang Yun-Hai Luo Tong Mou Ting-Ting Li Zhong-Tang Li Xu-fu Wei Zhong-Jun Wu Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Anatomy Basic Medical CollegeChongqing Medical UniversityChongqing 400016China

Background: Hepatic ischemia-reperfusion(I/R) injury(IRI) represents a crucial challenge in liver transplantation. Fisetin has anti-inflammatory, anti-aging and anti-oxidative properties. This study aimed to examine whether fisetin mitigates hepatic IRI and examine its underlying mechanisms. Methods: Sham or warm hepatic I/R operated mice were pretreated with fisetin(5, 10 or 20 mg/kg). Hepatic histological assessments, TUNEL assays and serum aminotransferase measurements were performed. An in vitro hypoxia/reoxygenation(H/R) model using RAW264.7 macrophages pretreated with fisetin(2.5, 5 or 10 μmol/L) was also used. Serum and cell supernatant concentrations of interleukin-1 β(IL-1 β), IL-18 and tumor necrosis factor-α(TNF-α) were determined by enzyme-linked immunosorbent assay(ELISA). Protein levels of p-GSK3 β, p-AMPK and NLR family pyrin domain-containing 3(NLRP3)-associated proteins were detected by Western blotting. Results: Compared with the I/R group, fisetin pretreatment reduced pathological liver damage, serum aminotransferase levels, serum concentrations of IL-1 β, IL-18 and TNF-α in the murine IRI model. Fisetin also reduced the expression of NLRP3 inflammasome-associated proteins(NLRP3, cleaved caspase-1, IL-1 β and IL-18) in I/R-operated liver. The experiments in vitro showed that fisetin decreased the release of IL-1 β, IL-18 and TNF-α, and reduced the expression of NLRP3 inflammasome-associated proteins in H/R-treated RAW264.7 cells. Moreover, fisetin increased the expressions of p-GSK3 β and p-AMPK in both models, indicating that its anti-inflammatory effects were dependent on GSK3 β/AMPK signaling. The antiinflammatory effects of fisetin were partially inhibited by the AMPK specific inhibitor compound C. Conclusions: Fisetin showed protective effects against hepatic IRI, countering inflammatory responses through mediating the GSK3 β/AMPK/NLRP3 inflammasome pathway.

关键词： Fisetin Hepatic ischemia-reperfusion injury GSK3βAMPK NLRP3

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Preparation and characterization of m PEG grafted chitosan micelles as 5-fluorouracil carriers for effective anti-tumor activity

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Chinese Chemical Letters 2014年第11期25卷 1435-1440页

作者： Dong-Jun fu Yu Jin Mu-Qing Xie Ya-Jing Ye Dong-Dong Qin Kai-Yan Lou Yan-zuo Chen Feng Gao Department of Pharmaceutics School of Pharmacy East China University of Science and Technology Shanghai Key Laboratory of functional Materials Chemistry East China University of Science and Technology Shanghai Key Laboratory of New Drug Design East China University of Science and Technology

The objective of this study was to investigate the potential of methoxy polyethylene glycol（m PEG）grafted chitosan（m PEG-g-CS） to be used as a drug carrier. m PEG-g-CS was successfully synthesized by one-step method with formaldehyde. The substitution degree of m PEG on chitosan was calculated by elemental analysis and was found to be（3.23 0.25）%. m PEG-g-CS self-assembled micelles were prepared by ultrasonic method with the controlled size of 178.5–195.1 nm and spherical morphology. Stable dispersion of the micelles was formed with the zeta potential of 2.3–30.2 m V. 5-Fluorouracil（5-fu）, an anticancer chemotherapy drug, was used as a model drug to evaluate the efficiency of the new drug delivery carrier. The loading efficiency of 5-fu was（4.01 0.03）%, and the drug-loaded m PEG-g-CS self-assembled micelle showed a controlled-release effect. In summary, the m PEG-g-CS self-assembled micelle is proved to be a promising carrier with controlled particle size and controlled-release effect. Therefore, it has great potential for the application as 5-fu carriers for effective anti-tumor activity.

关键词： Self-assembled micelles Methoxy polyethylene glycol grafted chitosan 5-Fluorouracil Controlled-release

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Effect of heat treatment on microstructure,mechanical and tribological properties of in-situ (TiC+TiB)/TC4 composites by casting

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China Foundry 2023年第3期20卷 207-217页

作者： Bo-wen Zheng Shuai Chen Chun-yu Yue Xue-jian Lin fu-yu Dong Hong-jun Huang Xiao-jiao zuo Yin-xiao Wang Xiao-guang Yuan School of Materials Science and Engineering Shenyang University of TechnologyShenyang 110870China

To enhance the performance of in-situ synthesized 6vol.%(Ti C+Ti B)/TC4 titanium matrix composites fabricated by casting,a variety of heat treatment processes were carried *** conducting microstructure observations following various heat treatments,it was found that the composites exhibit a basketweave microstructure,consisting of an α phase and a transformed β *** sizes of(α+β) phases were found to be refined to varying degrees after the heat treatment processes,while the morphology of Ti B remains largely unchanged and Ti C becomes *** testing revealed that all composites subjected to different heat treatments demonstrate a notable increase in ultimate compressive strength as well as a slight improvement in plasticity compared to the as-cast *** results of the tribological performance test indicated that the heat-treated composites exhibit lower average friction coefficient,specific wear rate,and worn surface roughness compared to the as-cast *** the heat treatment processes studied,the composite solution heated at 1,150 °C/1 h followed by air cooling,then 950 °C/1 h followed by air cooling,and finally 500 °C/4 h followed by air cooling,demonstrates the highest levels of hardness,compressive strength,and wear *** improvements are attributed to the combined effects of solid solution strengthening,grain refinement,and the pinning of dislocation slip.

关键词： titanium matrix composites heat treatment mechanical properties tribological properties

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Fegeneration of functional alveoli by adult human SOX9^＋ airway basal cell transplantation

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Protein & Cell 2018年第3期9卷 267-282页

作者： Qiwang Ma Yu Ma Xiaotian Dai Tao Ren Yingjie fu Wenbin Liu Yufei Han Yingchuan Wu Yu Cheng Ting Zhang Wei zuo Shanghai Pulmonary Hospital School of Medicine Tongji University Shanghai 200433 China Southwest Hospital Third Military Medical University of PLA Chongqing 400038 China Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai 200233 China The Institute for Biomedical Engineering and Nano Science School of Medicine Tongji University Shanghai 200029 China Kiangnan Stem Cell Institute Zhejiang 311300 China Guangzhou Institute of Respiratory disease The First Affiliated Hospital of Guangzhou Medical University Guangzhou 510120 China

Irreversible destruction of bronchi and alveoli can lead to multiple incurable lung diseases. Identifying lung stem/progenitor cells with regenerative capacity and utilizing them to reconstruct functional tissue is one of the biggest hopes to reverse the damage and cure such diseases. Here we showed that a rare population of SOX9^＋ basal cells （BCs） located at airway epithelium rugae can regenerate adult human lung. Human SOX9^＋ BCs can be readily isolated by bronchoscopic brushing and indefinitely expanded in feeder-free condition. Expanded human SOX9^＋ BCs can give rise to alveolar and bronchiolar epithelium after being transplanted into injured mouse lung, with air-blood exchange system reconstructed and recipient＇s lung function improved. Manipulation of lung microenvironment with Pirfenidone to suppress TGF-β signaling could further boost the transplantation efficiency. Moreover, we conducted the first autologous SOX9^＋ BCs transplantation clinical trial in two bronchiectasis patients. Lung tissue repair and pulmonary function enhancement was observed in patients 3-12 months after cell transplantation. Alto- gether our current work indicated that functional adult human lung structure can be reconstituted by orthotopic transplantation of tissue-specific stem/progenitor cells, which could be translated into a mature regenerative therapeutic strategy in near future.

关键词： lung regeneration transplantation stem cell. bronchiectasis alveoli

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