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缺血性脑卒中铁死亡特征基因NFE2L2的鉴定与验证

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中国组织工程研究 2025年第7期29卷 1466-1474页

作者：王咪马书杰刘杨齐瑞上海中医药大学附属岳阳中西医结合医院康复医学科上海市200437 上海中医药大学上海市201203 上海市第二康复医院上海市200431

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血... 详细信息

背景:铁死亡与缺血性脑卒中的发病密切相关,靶向铁死亡是一种治疗缺血性脑卒中有前景的方案,但具体调控靶点尚不明确。目的:通过生物信息学和机器学习方法筛选缺血性脑卒中铁死亡相关特征基因,并通过细胞实验进行验证,探讨铁死亡在缺血性脑卒中的作用。方法:基于GEO数据库和FerrDb数据库选取符合条件的缺血性脑卒中相关数据集和铁死亡表达数据集,通过t检验筛选铁死亡相关差异基因。对铁死亡相关差异基因进行GO功能富集分析与KEGG信号通路富集分析。通过PPI网络分析和机器学习筛选缺血性脑卒中铁死亡的特征基因,利用ROC分析和GSEA分析探究特征基因的准确性和生物功能。然后进行细胞实验,将HT22细胞分为对照组与缺血性脑卒中组,对照组不作任何干预,缺血性脑卒中组加入0.1 mol/L的H_(2)O_(2)干预24 h诱导细胞氧化应激和铁死亡,通过实时荧光定量RT-PCR和Western Blot验证铁死亡的发生和特征基因表达。结果与结论:(1)共获取45个铁死亡相关差异基因,GO和KEGG富集分析发现差异基因与氧化应激、自噬、铁死亡、脂肪细胞因子信号通路和线粒体代谢密切相关。(2)通过PPI网络中的MCODE插件和cytoHubba插件与机器学习中的LASSO算法和SVM-RFE算法共鉴定出1个铁死亡特征基因核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,NFE2L2)。(3)对NFE2L2进行ROC曲线分析,发现在训练集和验证集中构建的诊断预测模型具有良好的准确性与特异性;对NFE2L2进行GSEA分析,发现特征基因通过免疫、炎症反应、氨基酸代谢及神经因子调控等方面参与缺血性脑卒中发病机制的调控。(4)细胞实验的RT-PCR和Western Blot分析表明,与对照组对比,缺血性脑卒中组中的酰基辅酶A合成酶长链家族成员4 mRNA和蛋白表达水平显著增高(P<0.05),谷胱甘肽过氧化物酶4 mRNA和蛋白表达水平显著降低(P<0.05);与对照组对比,缺血性脑卒中组中特征基因NFE2L2 mRNA和蛋白表达水平显著增高(P<0.05)。(5)上述结果证实,缺血性脑卒中与铁死亡密切相关,靶向特征基因NFE2L2可以为研究和治疗缺血性脑卒中提供一定的思路与方向。

关键词：缺血性脑卒中铁死亡生物信息学 HT22细胞机器学习特征基因细胞实验 NFE2L2 ASCL4 GPX4

Tunable Luminescence Properties and Elucidating the Electronic Structures of Single-Phase Spherical BaWO 4:Dy^(3+),Tm^(3+),Eu^(3+)Phosphors for Warm-White-Lighting

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Journal of Renewable Materials 2022年第2期10卷 431-451页

作者： zun Bi Ke Jia Yunfei liu Yinong Lyu State Key Laboratory of Materials–Orient Chemical Engineering College of Materials Science and EngineeringNanjing Tech UniversityNanjingChina Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM) NanjingChina Jiangsu Collaborative Innovation Center for Advanced Inorganic Function Composites NanjingChina

A series of uniform single-phase spherical BaWO 4:Dy^(3+),Tm^(3+),Eu^(3+)phosphors were prepared via a microwave hydrothermal method by using trisodium citrate dehydrate as *** phase structure,morphology and photoluminescence properties were measured by powder X-ray diffraction,scanning electron microscope and fluorescence spectrometer,*** results show that uniform spherical microcrystals with diameters in the range of 2–4μm are *** the phase and morphology of samples are not significantly changed by doping rare earth(RE^(3+))*** the excitation wavelength of 356 and 365 nm,the samples BaWO 4:0.03Dy^(3+),yTm^(3+)can emit cold white *** order to lower the correlated color temperature(CCT)to get a warm white light,the Eu^(3+)ions were doped into BaWO 4:0.03Dy^(3+),0.01Tm^(3+).Especially,under the excitation of 365 nm,BaWO 4:0.03Dy^(3+),0.01Tm^(3+),0.03Eu^(3+)phosphor shew a bright warm white light with color coordi-nate of(0.4013,0.3629)and CCT of 3288 ***,in the BaWO 4:Dy^(3+),Tm^(3+),Eu^(3+)phosphors,the energy transfer mechanism among Dy^(3+),Tm^(3+)and Eu^(3+)ions have been discussed and the change of electron structures have been calculated by first-principles *** results shew that the uniform single-phase spherical BaWO 4:Dy^(3+),Tm^(3+),Eu^(3+)phosphors could be favorable candidates in warm white LEDs.

关键词： Barium tungstate microwave hydrothermal method spherical warm white lighting electron structures

Periodic Solutions for Two Coupled Nonlinear-Partial Differential Equations

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Communications in Theoretical Physics 2007年第3X期48卷 425-427页

作者： liu Shi-Da FU zun-Tao liu Shi-Kuo School of Physics Peking University Beijing 100871 China State Key Laboratory for Turbulence and Complex Systems Peking University Beijing 100871 China

In this paper, by applying the Jacobi elliptic function expansion method, the periodic solutions for two coupled nonlinear partial differential equations are obtained.

关键词： Jacobi elliptic function periodic wave solution nonlinear partial differential equation

The Two Main Mechanisms of Glacier Lake Outburst Flood in Tibet,China

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Journal of Mountain Science 2013年第2期10卷 239-248页

作者： liu Jing-jing TANG Chuan CHENG zun-lan Key Laboratory of Mountain Hazards and Surface Process Institute of Mountain Hazards and EnvironmentChinese Academy of Sciences State Key Laboratory of Geohazard Preventions Graduate School of Chinese Academy of Sciences

With the global warming,the disasters of Glacier Lake Outburst Flood(GLOF) have taken place frequently in Tibet in recent years and attracted more and more attention.A systematic survey was conducted on the 19 GLOFs in Tibet to study their two main *** indicated that all the events occurred in end-moraine lakes,and the outburst occurred partially and *** the breach had the shape of an arc or a trapezoid in overflow outburst and its top width was 3-5 times more than the *** two main mechanisms of GLOFs in Tibetan end-moraine Lake were overflow and piping,and the overflow mechanism caused by iceberg collapse was dominated in most cases.A formula was proposed to calculate the critical thickness of iceberg tongue that determines the *** analysis of the moraine materials revealed that seepage deformation is crucial in the outburst ***,we conducted a case study of the Guangxiecuo Lake to show its possible process of outburst and estimated the peak discharge of the resulted flood.

关键词： Glacier Lake Outburst Flood End-moraine Lake Outburst mechanism Overflow Piping Tibet

Envelope Periodic Solutions to One-Dimensional Gross-Pitaevskii Equation in Bose-Einstein Condensation

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Communications in Theoretical Physics 2009年第6期51卷 1069-1072页

作者： liu Shi-Kuo GAO Bin FU zun-Tao liu Shi-Da School of Physics Peking University Beijing 100871 China Department of Physics Yuxi Normal University Yuxi 653100 China

In this paper, applying the dependent and independent variables transformations as well as the Jacobi elliptic function expansion method, the envelope periodic solutions to one-dimensional Gross-Pitaevskii equation in... 详细信息

关键词： Gross-Pitaevskii equation transformations Jacobi elliptic function expansion method

QSPR Studies on lgK_(ow) and lgK_(oc) of Fluorobenzenes and Property Parameters Based on HF and DFT Calculations

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Chinese Journal of Structural Chemistry 2006年第7期25卷 823-829页

作者： ZHANG Xing-Chuan YU Jing WANG zun-Yao liu Hong-Xia College of Biological and Chemical Engineering Jiaxing University Zhejiang 314001 China Department of Material and Chemical Engineering Guilin Institute of Technology Guilin Guangxi 541004 China

Quantum chemistry parameters of 22 fluorobenzenes were computed at six levels using Hartree-Fock and DFT methods. Based on the experimental data of n-octanol/water partition coefficient （lgKow）, a three-parameter （dipole moments （μ）, zero point energy （ZPE） and free energy （G°）） quantitative correlation equation that can predict IgKow was developed using structural and thermodynamic parameters as theoretical descriptors. Similarly, based on experimental data of soil organic carbon sorption coefficient （lgKoc）, the other three-parameter （the most negative atomic net charge of molecule （q^-）, dipole moments （μ） and molecular volume （Vi）） quantitative correlation equation that can predict lgKoc was given. Quantitative correlation equations based on B3LYP/6- 311 G^＊＊ calculation were validated by VIF （variance inflation factors） and t-test and used to predict IgKow and lgKoc of a series of compounds. The result showed that the correlation and prediction ability of lgKoc equations based on three levels of HF/STO-3G, B3LYP/6-31 G^＊ and B3LYP/6- 311G^＊＊ are all more advantageous than those based on AMI.

关键词： fluorobenzene linear solvation energy theory Hartree-Fock density functional theory QSPR n-octanol/water partition coefficient (lgKow) soil organic carbon sorption coefficient (lgKoc)

The Homoclinic Orbit Solution for Functional Equation

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Communications in Theoretical Physics 2002年第5期38卷 553-554页

作者： liu Shi-Da FU zun-Tao liu Shi-Kuo REN Kui School of Physics Peking University Beijing 100871 China STCS Peking University Beijing 100871 China School of Physics Peking University Beijing 100871 China STCS Peking University Beijing 100871 China School of Physics Peking University Beijing 100871 China School of Physics Peking University Beijing 100871 China

In this paper, some examples, such as iterated functional systems, scaling equation of wavelet transform,and invariant measure system, are used to show that the homoclinic orbit solutions exist in the functional equat... 详细信息

关键词： functional equation, scaling equation, solitary wave, homoclinic orbit

New Rational Form Solutions to Coupled Nonlinear Wave Equations

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Communications in Theoretical Physics 2005年第2X期44卷 235-242页

作者： FU zun-Tao LIN Guang-Xing liu Shi-Kuo liu Shi-Da School of Physics Peking University Beijing 100871 China State Key Laboratory for Turbulence and Complex Systems Peking University Beijing 100871 China

The new rational form solutions to the elliptic equation are shown, and then these solutions to the elliptic equation are taken as a transformation and applied to solve nonlinear coupled wave equations. It is shown that more novel kinds of solutions are derived, such as periodic solutions of rational form, solitary wave solutions of rational form,and so on.

关键词： elliptic equation Jacobi elliptic function nonlinear coupled equations periodic wave solution rational form

Studies of n-Octanol/water Partition Coefficients (lgK_(ow)) for Organophosphate Compounds by Density Functional Theory

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Chinese Journal of Structural Chemistry 2007年第3期26卷 367-373页

作者： liu Hong-Xia WANG zun-Yao ZHAI Zhi-Cai liu Hong-Yan WANG Lian-Sheng Department of Material and Chemical Engineering Guilin Institute of Technology Guangxi 541004 China School of Biological and Chemical Engineering Jiaxing University Zhejiang 314001 China State Key Laboratory of Pollution Control and Resources Reuse School of the Environment Nanjing University Nanjing 210093 China

Optimized calculation of 35 dialkyl phenyl phosphate compounds （OPs） was carded out at the B3LYP/6-31G^＊ level in Gaussian 98 program. Based on the theoretical linear solvation energy relationship （TLSER） model, the obtained parameters were taken as theoretical descriptors to establish the novel QSPR model for predicting n-octanol/water partition coefficients （lgKow） of OPs. The new model achieved in this work contains three variables, i.e., molecular volume （Vm）, dipole moment of the molecules （μ） and enthalpy （H^0）. For this model, R^2 = 0.9167 and SD = 0.31 at large t values. In addition, the variation inflation factors （VIF） of variables are all close to 1.0, suggesting high accuracy of the predicting model. And the results of cross-validation test （q^2 = 0.8993） and method validation also showed the model of this study exhibited optimum stability and better predictive power than that from semi-empirical method. The model achieved can be used to predict IgKow of congeneric compounds.

关键词： organophosphate compounds (OPs) n-octanol/water partition coefficients (Kow) quantitative structure-property relationship (QSPR) density functional theory (DFT)