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Molecular characterization of sweet potato(Ipomoea batatas[L.]Lam)germplasms for desirable traits by using simple sequence repeats markers

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BIOCELL 2023年第1期47卷 227-237页

作者： KHANDAKAR ABUMDMOSTAFIZAR RAHMAN ABDUL SHUKOR JURAIMI MDREZWAN MOLLA MUHAMMAD ASYRAFMD HATTA zulkefly BIN sulaiman SHAMIMA SULTANA AHMED GABER BENUKAR BISWAS AKBAR HOSSAIN Tuber Crop Research Centre Bangladesh Agricultural Research InstituteGazipur1701Bangladesh Department of Crop Science Faculty of AgricultureUniversiti Putra MalaysiaUPMKuala Lumpur43400Malaysia Plant Genetic Resources Centre Bangladesh Agricultural Research InstituteGazipur1701Bangladesh Department of Agriculture Technology Faculty of AgricultureUniversiti Putra MalaysiaUPMKuala Lumpur43400Malaysia Regional Agricultural Research Station Bangladesh Agricultural Research InstituteCumilla3500Bangladesh Department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia Department of Agronomy Bidhan Chandra Krishi ViswavidyalayaNadiaWB741252India Division of Agronomy Bangladesh Wheat and Maize Research InstituteDinajpur5200Bangladesh

Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic ***,molecular characterization of germplasms is important to obtain target cultivars with desirable *** potato[Ipomoea batatas(L.)Lam]is widely considered the world’s most important crop,with great diversity in morphological and phenotypic *** genetic diversity of 20 sweet potato germplasms originating from Bangladesh,CIP,Philippines,Taiwan,and Malaysia were compared,which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and *** information was effective in differentiating or clustering the sweet potato genotypes.A total of 64 alleles were generated using the 20 primers throughout the 20 germplasm samples,with locus IBS97 having the highest number of alleles(5),whereas locus IbU33 had the fewest alleles(2).The alleles varied in size from 105(IbU31)to 213 base pairs(IBS34).The Polymorphism Information Content(PIC)values for the loci IbL46 and IBS97 varied from 0.445 to ***97 has the highest number of effective alleles(3.704),compared to an average of *** average Shannon’s diversity index(H)was 1.003,ranging from 0.673 in IbU3 to 1.432 in *** value of gene flow(Nm)varied between 0.000 and 0.005,with an average of 0.003,whereas genetic differentiation(FST-values)ranged between 0.901 and *** sweet potato germplasm included in this study had a broad genetic ***1 ***9 and SP12 ***18 germplasm pairings had the greatest genetic distance(GD=0.965),while SP1 ***2 germplasm couples had the least genetic diversity(GD=0.093).Twenty genotypes were classified into two groups in the UPGMA dendrogram,with 16 genotypes classified as group“A”and the remaining four genotypes,SP10,SP18,SP19,and SP20,classified as group“B.”According to cluster analysis,the anticipated heterozygosity(gene diversity)of Nei

关键词： Sweet potato Simple sequence repeats(SSRs) Genetic diversity Dendrogram

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Assessment of <i>Stevia rebaudiana</i>Bertoni Genotypes via Morpho-Agronomic Traits under Two Light Conditions

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American Journal of Plant Sciences 2018年第7期9卷 1403-1413页

作者： Dhulfiqar Aamer Abdulameer Mohamad Bin Osman zulkefly sulaiman Mohd Rafi Yusop Shamsiah Abdullah Parisa Azizi Qusay Abdulhamza Muttaleb Department of Crop Science Faculty of Agriculture Universiti Putra Malaysia Serdang Malaysia Institute of Plantation Studies Universiti Putra Malaysia Serdang Malaysia Laboratory of Food Crops Institute of Tropical Agriculture Universiti Putra Malaysia Serdang Malaysia Faculty of Plantation and Agrotechnology Universiti Teknologi MARA Shah Alam Malaysia Technical Institute of Babel Al-Furat Al-Awsat Technical University Al Hillah Iraq

Stevia rebaudiana Bertoni, a non caloric natural sweetner is currently the available substitute to sugar. It is a genus of about 150 species of herbs and shrubs, a compositae and native to Paraguay. However, stevia is a short day plant with almost a constant day length 12 hours in Malaysia stevia generated to flower early. Therefore, the aim of the study was to investigate the effect of different day length on Stevia rebaudiana genotypes in Malaysia. The experiment was undertaken during December 2016 to April 2017 in the Faculty of Design and Architecture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Eight stevia genotypes namely (CHV1, Morita2, MS012, MS012 (4x), SA18, TPU, and hybrids IIUM-F1 and UiTM-H2) were evaluated for the agronomic traits under two conditions: sun light (14 hours) using a factorial experiment. The agronomic performance of Stevia rebaudiana was significantly in long day length (>14 hours) environment comparing with short day length (14 hours) environments.

关键词： Stevia rebaudiana Propagation Sun Light Simulated Light

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