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Impact of seepage flow on sediment resuspension by internal solitary waves:parameterization and mechanism

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Journal of Oceanology and Limnology 2023年第2期41卷 444-457页

作者： Zhuangcai TIAN Chao liu ziyin REN Xiujun GUO Mingwei ZHANG Xiuhai WANG Lei SONG Yonggang JIA State Key Laboratory for Geomechanics and Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China Key Laboratory of Coastal Science and Integrated Management Ministry of Natural ResourcesQingdao 266061China Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering Ocean University of ChinaQingdao 266100China

Sediment incipient motion is the first step in sediment *** studies ignored the effect of seepage flow on the mobility of sediment particles and simplified the seabed surface as a rigid boundary.A flume experiment was designed to innovatively divide the seabed into two parts to control the dynamic response of the seabed and control the seepage *** the experiment,the seabed sediments and the amplitude of internal solitary waves(ISWs)were changed to compare and analyze the impact of seepage flow on the sediment resuspension by shoaling ***,parametric research and verification were carried *** indicate that seepage flow can greatly influence fine sand,promote sediment resuspension,and increase the amount of suspension by two times on ***,seepage flow had a little effect on the suspension of clayey silt and sandy ***,seepage force was added to the traditional gravity,drag force,and uplift force,and the parameterization of threshold starting shear stress of coarse-grained sediments was *** results of this parameterization were verified,and seepage force was critical to *** threshold starting shear stress was reduced by 54.6%after increasing the seepage *** physical mechanism of this process corresponded to the vertical reciprocating transient seepage in and out the seabed interface caused by the wave-induced transient excess pore water *** quantitative study on seepage flow for shear stress of coarse-grained sediments induced by ISWs is critical to geohazard assessment.

关键词： internal waves seepage flow sediment resuspension parameterization mechanism

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GB7 acetate,a galbulimima alkaloid from Galbulimima belgraveana,possesses anticancer effects in colorectal cancer cells

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Journal of Pharmaceutical Analysis 2022年第2期12卷 339-349页

作者： ziyin Li Lianzhi Mao Bin Yu Huahuan liu Qiuyu Zhang Zhongbo Bian Xudong Zhang Wenzhen Liao Suxia Sun Department of Nutrition and Food Hygiene Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou510515China School of Pharmaceutical Sciences Zhengzhou UniversityZhengzhou450000China

GB7 acetate is a galbulimima alkaloid obtained from Galbulimima ***,information regarding its structure,biological activities,and related mechanisms is not entirely available.A series of spectroscopic analyses,structural degradation,interconversion,and crystallography were performed to identify the structure of GB7 *** MTT assay was applied to measure cell proliferation on human colorectal cancer HCT 116 *** expressions of the related proteins were measured by Western *** electron microscopy(TEM),acridine orange(AO)and monodansylcadaverine(MDC)staining were used to detect the presence of autophagic vesicles and autolysosomes.A transwell assay was performed to demonstrate metastatic *** consumption rate(OCR)and extracellular acidification rate(ECAR)assays were performed to determine the mitochondrial oxidative phosphorylation(OXPHOS)and glycolysis activity of HCT 116 *** data showed that GB7 acetate suppressed the proliferation and colony-forming ability of HCT 116 *** with GB7 acetate significantly induced the formation of autophagic vesicles and ***7 acetate upregulated the expressions of LC3 and Thr172 phosphorylated adenosine 5'-monophosphate(AMP)-activated protein kinase a(p-AMPKa),which are key elements of *** addition,GB7 acetate suppressed the metastatic capabilities of HCT 116 ***,the production of matrix metallo-proteinase-2(MMP-2)and MMP-9 was reduced,whereas the expression of E-cadherin(E-cad)was ***,GB7 acetate significantly reduced mitochondrial OXPHOS and *** conclusion,the structure of the novel Galbulimima alkaloid GB7 acetate was ***7 acetate was shown to have anti-proliferative,pro-autophagic,anti-metastatic,and anti-metabolite capabilities in HCT 116 *** study might provide new insights into cancer treatment efficacy and cancer chemoprevention.

关键词： GB7 acetate Galbulimima belgraveana Anti-cancer effects HCT 116 cells

基于PubMed数据库的临床药学工作切入点的文献计量分析

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中国医院用药评价与分析 2024年第4期24卷 506-509页

作者：程海新马子寅刘碧晴钟旭丽张建民首都儿科研究所附属儿童医院药学部北京100020 首都医科大学科技处北京100069

目的:基于文献可视化分析,探讨临床药学研究的发展趋势。方法:利用BICOMB 2.0和VOSviewer 1.6.16软件,对PubMed数据库自建库起至2023年4月7日发表的临床药学相关文献进行提取统计和可视化分析。结果:最终纳入文献4 609篇,其中我国学者... 详细信息

目的:基于文献可视化分析,探讨临床药学研究的发展趋势。方法:利用BICOMB 2.0和VOSviewer 1.6.16软件,对PubMed数据库自建库起至2023年4月7日发表的临床药学相关文献进行提取统计和可视化分析。结果:最终纳入文献4 609篇,其中我国学者发文量为174篇。我国学者总体发文量呈递增趋势;发文量较多的机构包括中国药科大学、浙江大学医学院附属第一医院、北京大学医学部、上海交通大学医学院附属仁济医院以及首都医科大学附属北京朝阳医院。国内临床药学研究热点问题较单一,涉及医院药学服务、成年人的用药管理和回顾性研究等领域。结论:临床药学在我国起步晚但发展迅速,发展前景广阔。

关键词：临床药学 PubMed数据库文献计量学可视化分析热点与前沿

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银杏二萜内酯葡胺注射液对缺血性脑卒中小鼠黑质脑区的调控机制研究

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中草药 2024年第11期55卷 3735-3748页

作者：刘子宇徐欣玉吕文欣郭志宏张新庄曹亮王振中田金洲王团结潘贤武子寅肖伟南京中医药大学中药制药过程控制与智能制造技术全国重点实验室江苏南京210023 江苏康缘药业股份有限公司江苏连云港222001

目的研究银杏二萜内酯葡胺注射液(Diterpene Ginkgolides Meglumine Injection,DGMI)在C57BL/6J小鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型黑质脑区中抗脑缺血再灌注损伤的潜在作用通路。方法C57BL/6J小鼠按随机数字表分为假手术组、模型组、DGMI(25 mg/kg)组及银杏叶提取物761(Ginkgo biloba extract-761,EGb-761,100 mg/kg)组,构建MCAO模型,通过测定各组小鼠术后24 h的改良版神经功能缺损评分和脑梗死率,评价DGMI相较于EGb-761治疗缺血性脑卒中的疗效。进一步利用Illumina二代测序平台对各组小鼠脑组织黑质样本进行高通量转录组测序,并结合基因集富集分析(gene set enrichment analysis,GSEA)、差异表达基因(differentially expressed genes,DEGs)的基因本体(gene ontology,GO)功能和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析等生物信息学分析方法,评价DGMI抗脑缺血再灌注损伤作用及潜在作用机制。采用qRT-PCR验证转录组测序中关键基因表达。结果与假手术组比较,模型组改良版神经功能缺损评分和脑梗死率显著升高(P<0.001);与模型组比较,DGMI组治疗效果与EGb-761接近,可显著改善改良版神经功能缺损评分和脑梗死率(P<0.01、0.001)。转录组的数据降维分析显示,模型组与假手术组样本显著分离。GSEA结果显示,模型组与假手术组对比显著富集35条通路,DGMI组相较于模型组可有效逆转7条通路。与假手术组相比,模型组共筛选出88个DEGs,将其与他人发现的大小鼠MCAO模型组共同差异基因比较(15个),有10个基因相同。模型组GO功能分析主要富集在炎症和细胞趋化等过程,KEGG通路分析主要富集在细胞因子互作、肿瘤坏死因子等信号通路。与模型组相比,DGMI组DEGs的GO功能分析主要富集在神经信号传导过程,KEGG通路分析主要富集在多巴胺能信号通路、神经活性受体配体作用等。qRT-PCR验证与转录组结果基本一致。结论DGMI可有效抵抗脑缺血再灌注损伤,发挥作用的关键基因及信号通路主要集中于抗炎、调节神经突触等生物学过程,可能通过改善多巴胺能通路治疗损伤。

关键词：银杏二萜内酯葡胺注射液缺血性脑卒中黑质大脑中动脉闭塞模型转录组测序

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A Hierarchically Porous Metal-Organic Framework from Semirigid Ligand for Gas Adsorption

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Chinese Journal of Chemistry 2016年第2期34卷 215-219页

作者： Lizhen liu Yingxiang Ye Zizhu Yao liuqin Zhang ziyin Li Lihua Wang Xiuling Ma Qian-Huo Chen Zhangjing Zhang Shengchang Xiang College of Chemistry and Chemical Engineering Fujian Provincial Key Laboratory of Polymer Materials Fujian Normal University Fuzhou Fujian 350007 China State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fuzhou Fujian 350002 China

Hierarchically porous materials play an important role in facilitating mass transport and improving efficiency of adsorption and separation processes. In this paper, a new strategy is proposed to realize a hierarchically porous metal-organic framework （[Cu2（OH）（L）]＇（DMF）0.8 （FJU-11, H3L=3,5-（4-carboxybenzyloxy）benzoic acid, DMF= N,N-dimethylformamide） via using semi-rigid multi-carboxylic acids. Interestingly, FJU-11 possesses the large adsorption capacities and small isosteric heats toward CO2. The column breakthrough experiment for FJU-11 highlights its potential application in the separation of the flue gas.

关键词： metal-organic frameworks hierarchical pore semirigid ligand gas sorption column breakthrough experiment

金振口服液治疗支气管哮喘的药效作用和分子机制

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药物评价研究 2024年第7期47卷 1486-1501页

作者：董佳裕刘鑫苏真真邢薇薇徐欣玉徐利杨钰冰李美瑶张新庄曹亮王振中武子寅肖伟南京中医药大学中药制药过程控制与智能制造技术全国重点实验室江苏南京210023 江苏康缘药业股份有限公司江苏连云港222001

目的建立小鼠支气管哮喘模型和人非小细胞肺癌细胞(A549)炎症模型,研究金振口服液(Jinzhen Oral Liquid,JZOL)对支气管哮喘的药效作用和分子机制。方法将66只雌性BALB/c小鼠随机分为对照组和模型组,模型组小鼠采用卵清蛋白(OVA)致敏法... 详细信息

目的建立小鼠支气管哮喘模型和人非小细胞肺癌细胞(A549)炎症模型,研究金振口服液(Jinzhen Oral Liquid,JZOL)对支气管哮喘的药效作用和分子机制。方法将66只雌性BALB/c小鼠随机分为对照组和模型组,模型组小鼠采用卵清蛋白(OVA)致敏法建立哮喘模型后,将其分为模型组和JZOL高、中、低剂量(4.4、2.2、1.1 mg·kg^(-1))组和地塞米松组(Dex,2 mg·kg^(-1))。全身体积描记系统测定小鼠气道反应Penh值。HE染色观察小鼠肺组织病理学情况。瑞氏-姬姆萨染色检测肺泡灌洗液(BALF)中炎性细胞数量。ELISA检测BALF中肿瘤坏死因子-α(TNF-α)和血清中免疫球蛋白E(IgE)和免疫球蛋白G1(IgG1)浓度。采用20 ng·mL^(-1)白细胞介素(IL)-1β诱导A549细胞24 h建立细胞炎症模型,随机分为对照组、模型组以及4个质量浓度JZOL(5.39、2.69、1.35、0.67 mg·mL^(-1))处理组。MTS法测定JZOL对A549细胞的药物毒性。采用RNA-seq技术对6个实验组进行转录组学分析,筛选差异基因,对其进行GSEA通路富集分析、京都基因与基因组百科全书(KEGG)通路富集分析和基因本体(GO)注释通路富集分析,通过实时荧光定量PCR(qRT-PCR)实验对筛选出的关键基因进行实验验证。结果体内实验表明,JZOL能够改善小鼠肺组织炎性细胞浸润,降低小鼠气道高反应性Penh值,减少BALF中巨噬细胞、嗜酸性粒细胞和中性粒细胞的数量,降低BALF中TNF-α和血清中IgE和IgG1的浓度。体外实验表明,JZOL对A549细胞的最大无毒浓度为5.39 mg·mL^(-1)。基因富集分析结果显示,与对照组相比,模型组共获得46条炎症模型相关通路,而JZOL不同浓度处理组对这些通路的逆转比例分别为34.8%(5.39 mg·mL^(-1))、50%(2.69 mg·mL^(-1))、32.6%(1.35 mg·mL^(-1))和26%(0.67 mg·mL^(-1))。差异表达基因(DEG)的KEGG富集分析显示,与对照组相比,模型组共富集到58条通路;与模型组相比,JZOL处理组共富集到32条通路,逆转了IL-17、TNF、核苷酸寡聚化结构域(NOD)样受体信号通路等。对IL-17信号通路中趋化因子配体1(CXCL1)、趋化因子配体2(CXCL2)、脂质运载蛋白2(LCN2)进行qRT-PCR验证,与转录组测序结果一致。结论JZOL对由OVA诱导的小鼠支气管哮喘模型具有显著的治疗功效,能有效缓解哮喘小鼠的气道高反应性并抑制肺部组织的炎症反应。其抗炎效果是通过调节CXCL1、CXCL2和LCN2这几个关键基因的表达,进而影响IL-17信号通路来实现的。

关键词：金振口服液支气管哮喘气道炎症气道高反应性 A549细胞转录组测序 qRT-PCR

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住院患者跌倒预防相关临床实践指南的质量评价及推荐意见总结

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护士进修杂志 2024年第4期39卷 395-400页

作者：刘加婷邓子银赵丽蓉张蕊成磊周士萍程云复旦大学附属华东医院上海200040 复旦大学护理学院上海200032

目的对国内外住院患者跌倒预防相关临床实践指南进行质量评价和推荐意见总结。方法系统检索国内外指南网、医学文献数据库以及专业学术网站,应用相应评价工具对纳入指南和专家共识进行质量评价,并汇总相关推荐内容。检索时限为建库至202... 详细信息

目的对国内外住院患者跌倒预防相关临床实践指南进行质量评价和推荐意见总结。方法系统检索国内外指南网、医学文献数据库以及专业学术网站,应用相应评价工具对纳入指南和专家共识进行质量评价,并汇总相关推荐内容。检索时限为建库至2022年12月13日。结果共纳入9篇指南和5篇专家共识,9篇指南在AGREE II 6个领域得分为范围和目的94.03%、清晰性73.86%、参与人员76.07%、严谨性80.19%、应用性69.34%、独立性63.88%,其中5篇为A级推荐、4篇为B级推荐;3篇专家共识中的质量评价结果中条目1~6均为“是”,其余2篇条目1~4均为“是”,条目5~6分别为“否”和“不清楚”,5篇专家共识质量评价结果均为“纳入”。经内容整理,住院患者跌倒预防相关内容包括环境改善、跌倒风险评估、跌倒干预及跌倒后处理、药物审查、物理训练、保护器具的使用、营养支持、健康宣教8个方面,28项内容。结论本研究纳入的住院患者跌倒预防指南整体质量较好但仍有待提高,对临床护理人员预防患者跌倒、提高护理质量有一定的指导意义。

关键词：住院患者跌倒预防指南质量评价意见总结循证护理

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STX5 Inhibits Hepatocellular Carcinoma Adhesion and Promotes Metastasis by Regulating the PI3K/mTOR Pathway

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Journal of Clinical and Translational Hepatology 2023年第3期11卷 572-583页

作者： Bin Zhang ziyin Zhao Youpeng Wang Tingting Guo Mingyang He Ge Guan Pai Peng Jinzhen Cai Bingyuan Zhang Xutao liu Qiaoling Song Key Laboratory of Marine Drugs Ministry of Education School of Medicine&PharmacyOcean University of ChinaQingdaoShandongChina Organ Transplantation Center The Affiliated Hospital of Qingdao UniversityQingdaoShandongChina Department of Hepatobiliary and Pancreatic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandongChina Samueli School of Engineering University of California Los AngelesLos AngelesCAUSA

Background and Aims:Syntaxin 5(STX5)is a member of the syntaxin or target-soluble SNAP receptor(t-SNARE)fam-ily and plays a critical role in ***,its function and molecular mechanism in tumor cell migration are still *** role of STX5 in influencing hepatocellular carci-noma(HCC)is an important topic in our ***:By using quantitative reverse transcription polymerase chain reaction(qPCR),western blotting,and immunohistochemical analysis of RNA and protein in tissues,we comprehensively evaluated data sets from public databases and clinical patient cohorts for *** correlation of STX5 expression with the clinicopathological characteristics of HCC patients were *** addition,we predicted signal pathways from dif-ferentially expressed genes(DEGs)and the Cancer Genome Atlas(TCGA)databases,and confirmed the prediction using integrated transcriptome and *** further investi-gated the underlying mechanisms of STX5 in the migration and adhesion of HCC cells both in vitro and in ***:In the TCGA dataset and our patient cohort,STX5 levels were significantly higher in HCC tissues than in adjacent normal liver *** the same time,high expression of STX5 pre-dicted worse prognosis in patients with liver *** ex-pression of STX5 indicates the decrease of adhesion and the increase of migration of HCC cells,and the conversion of epi-thelial-mesenchymal transition(EMT)in vitro via PI3K/mTOR pathway ***,when Sirolimus,a phospho-inositide 3-kinase(PI3K)/AKT/mechanistic target of rapa-mycin(mTOR)inhibitor acts on cells simultaneously,STX5 overexpression-mediated enhancement of HCC metastasis is ***-negative regulation of STX5 and mTOR further enhanced the inhibitory effect of STX5 on HCC *** vivo,STX5 knockdown inhibited the metastasis of HCC ***:Our study demonstrates a novel research result that STX5 promotes HCC metastasis through PI3K/mTOR *** believe th

关键词： Syntaxin 5 Hepatocellular carcinoma PI3K/mTOR Epithelial-mes-enchymal transition

Formation and emission of geraniol in tea(Camellia sinensis) leaves exposed to tea green leafhopper(Empoasca(Matsumurasca) onukii Matsuda) attack

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Formation and emission of geraniol in tea(Camellia sinensis)...