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Influence of environmental factors on arsenic accumulation and biotransformation using the aquatic plant species Hydrilla verticillata

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Journal of Environmental Sciences 2020年第4期32卷 244-252页

作者： Yuan Zhao zhuo Zhen Zhenhong Wang Liqing Zeng Changzhou Yan Key Laboratory of Urban Environment and Health Fujian Key Laboratory of Watershed EcologyInstitute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China School of Chemistry and Environment Fujian Province Key Laboratory of Modern Analytical Science and Separation TechnologyMinnan Normal UniversityZhangzhou 363000China

Hydrilla verticillata(waterthyme)has been successfully used for phytoremediation in arsenic(As)contaminated *** evaluate the effects of environmental factors on phytoremediation,this study conducted a series of orthogonal design experiments to determine optimal conditions,including phosphorus(P),nitrogen(N),and arsenate(As(Ⅴ))concentrations and initial pH levels,for As accumulation and biotransformation using this aquatic plant species,while also analyzing As species transformation in culture media after 96-hr *** of variance and the signal-to-noise ratio were used to identify both the effects of these environmental factors and their optimal conditions for this *** indicated that both N and P significantly impacted accumulation,and N was essential in As species *** N and intermediate P levels were critical to As accumulation and biotransformation by ***,while high N and low P levels were beneficial to As species transformation in culture *** highest total arsenic accumulation was(1.7.2±1..4)μg/g dry weight when As(V)was at level 3(375μg/L),N at level 2(4 mg/L),P at level 1.0.02 mg/L),and pH at level 2(7).Although *** is highly efficient in removing As(Ⅴ)from aquatic environments,its use could be potentially harmful to both humans and the natural environment due to its release of highly toxic *** cost-effective and ecofriendly phytoremediation of As-contaminated water,both N and P are helpful in regulating As accumulation and transformation in plants.

关键词： Phytoremediation Orthogonal design pH Phosphorus Nitrogen

坩埚尺寸和电磁频率对半固态A356铝合金浆料流动的影响

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金属学报 2018年第3期54卷 435-442页

作者：刘政陈志平陈涛江西理工大学机电工程学院赣州341000 江西理工大学材料科学与工程学院赣州341000

采用数值模拟方法,通过改变坩埚长短轴比例R和电磁搅拌频率研究半固态A356铝合金浆料的流动规律,以及R对半固态A356铝合金浆料初生相组织的影响。结果表明:随着R增大,半固态A356铝合金在短轴上所受的最大电磁力和最大流速呈先增大后减... 详细信息

采用数值模拟方法,通过改变坩埚长短轴比例R和电磁搅拌频率研究半固态A356铝合金浆料的流动规律,以及R对半固态A356铝合金浆料初生相组织的影响。结果表明:随着R增大,半固态A356铝合金在短轴上所受的最大电磁力和最大流速呈先增大后减小的趋势,在长轴上所受的最大电磁力和最大流速呈先增大后减小再增大的现象;频率越高,短轴和长轴上所受的电磁力差和流速差越明显,因而可使熔体流动时出现"加速-减速-加速"的循环运动现象。当电磁频率和R分别为30 Hz和1.1.,坩埚长轴和短轴上的最大流速分别为1.3.6和1.3.2 mm/s,流速差最小,此时可制备出较优的半固态A356铝合金浆料。

关键词：半固态铝合金坩埚尺寸电磁频率流速数值模拟

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

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Journal of Pharmaceutical Analysis 2010年第2期22卷 97-101页

作者： Qin Xiao1.2,Hui chen2,Jin-Ming Lin2 1. College of Ocean,Hebei Agricultural University,Qinhuangdao 066003 2. The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,Department of Chemistry,Tsinghua University,Beijing 1.0084,China. College of Ocean Hebei Agricultural University Qinhuangdao 066003 China Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Department of Chemistry Tsinghua University Beijing 100084 China

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase,equipped with a polyhistidine affinity tag,was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×1.8,and the specific activity was 2.9×1.1. RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein,the ATP content of bacteria was 9.48×1.-1.mol/mL,and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together,our results provided a simple and efficacious method of the preparation of recombinant luciferase,which could be applied in the determination of bacteria via ATP bioluminescence.

关键词： ATP bioluminescence bacterial detection expression firefly luciferase purification

Effects of chronic elevated intraocular pressure on parameters of optical coherence tomography in rhesus monkeys

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 542-548页

作者： Zhi-Chao Yan Xue-Jiao Yang Hong-Rui chen Shui-Feng Deng Ying-Ting Zhu Ye-Hong {1. Department of Ophthalmology the Second Affiliated Hospital of Guangzhou Medical University State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM: To determine the progression of parameters from optical coherence tomography(OCT) in chronic elevated intraocular pressure(IOP) ***: A chronic elevated IOP model of rhesus monkeys was induced by laser photocoagulation. Representative OCT parameters, including the average and four-quadrant retinal nerve fiber layer(RNFL) thickness, and parameters from optic nerve head(ONH) analysis were collected before and after laser treatments biweekly for up to 28 wk. The performance of each parameter for early progression detection was analyzed. The progressive trends toward elevated IOP were analyzed using a linear mixed-effects ***: There were 1. successfully maintained high IOP eyes in 7 monkeys. The follow-up time was 24±5.37 wk. With cumulative IOP elevation, the cup area, rim area and C/D area ratio were statistically significantly changed as early as 2 wk after elevated IOP induction(P<0.05). The quadrant RNFL thickness changed at 6wk after high IOP induction, and the superior and inferior RNFL thicknesses exhibited more obvious reductions than other quadrants. The average RNFL thickness was the last one to show a significant decrease at ***: The parameters of ONH are most sensitive to elevated IOP in a primate glaucomatous model. These findings suggest that we should focus on those parameters instead of RNFL thickness in patients with elevated IOP, as they might present with earlier glaucomatous changes.

关键词： optical coherence tomography rhesus monkey glaucoma high intraocular pressure retinal nerve fiber layer

Bardenpho-臭氧催化氧化-双膜法用于再生水厂

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中国给水排水 2024年第6期40卷 103-107页

作者：王秋晨姜红张勇陈娣山东省城建设计院山东济南250021 山东省建设建工集团消防工程有限公司山东济南250101 山东省工程咨询院山东济南250013

山东省某县经济开发区污水处理厂总规模为4.5×1.^(4 )m^(3)/d,一期采用A~2O生化池+气浮池+纤维束滤池工艺,二期采用调节池-涡凹气浮池-水解酸化池-A~2O生化池-二沉池-高效沉淀池—臭氧接触池-V型砂滤池工艺。现状一期和二期工程出水水... 详细信息

山东省某县经济开发区污水处理厂总规模为4.5×1.^(4 )m^(3)/d,一期采用A~2O生化池+气浮池+纤维束滤池工艺,二期采用调节池-涡凹气浮池-水解酸化池-A~2O生化池-二沉池-高效沉淀池—臭氧接触池-V型砂滤池工艺。现状一期和二期工程出水水质均能稳定达到《城镇污水处理厂污染物排放标准》(GB 1.91.-2002)的一级A标准。因服务范围内多家企业有再生水使用需求,通过改造二期工程生化系统及深度处理系统,使出水水质提标至地表准Ⅳ类水质后,再与一期出水勾兑进入再生水系统。二期改造采用Bardenpho及臭氧催化氧化工艺,再生水系统采用双膜法(UF+RO)进一步去除盐分。2022年通水以来,实际再生水量为1.530 m^(3)/d,再生水费用为2.0元/m^(3)。整个系统运行安全稳定,出水满足企业用水要求,效果良好。

关键词： Bardenpho工艺臭氧催化氧化 UF工艺 RO工艺再生水厂脱盐

Safety and efficacy of a novel responsive neurostimulation system in China for drug-refractory focal epilepsy:The first-in-man study

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Chinese Medical Journal 2024年第12期137卷 1486-1488页

作者： Kailiang Wang Yongzhi Shan Penghu Wei Liankun Ren Liang chen Junming Zhu Guoguang Zhao Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijing 100053China International Neuroscience Institute(China-INI) Clinical Research Center for EpilepsyCapital Medical UniversityBeijing 100053China China National Medical Center for Neurological Diseases Beijing 100053China Department of Neurology Xuanwu HospitalCapital Medical UniversityBeijing 100053China Department of Neurosurgery Huashan HospitalFudan UniversityShanghai 200032China Department of Neurosurgery The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310009China

To the Editor:Recent studies have characterized drugresistant epilepsy(DRE)as essentially a neural network disease.[1.Detection and disconnection of this pathological epileptic connectome or network can greatly improve seizure *** team also proposes the development of the newly emerging branch of epileptic networks neurosurgery(ENN).Responsive neurostimulation(RNS)is an ENN-oriented emerging treatment option without the resection of the seizure-onset zone or epileptic focus and aims to control the seizure or other epileptic manifestations by modulating or disrupting the network’s key nodes(epileptic hubs)in a self-responsive way.

关键词： epilepsy stimulation drug

Amplifying protection against acute lung injury:Targeting both inflammasome and cGAS-STING pathway by Lonicerae Japonicae Flos-Forsythiae Fructus drug pair

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Chinese Herbal Medicines 2024年第3期16卷 422-434页

作者： Junjie Li Ming Dong Qing Yao Xu Dong Yuanyuan chen Jincai Wen Yingjie Xu Zhixin Wu Xiaomei Zhao Ye Xiu Xiaoyan Zhan Zhaofang Bai Xiaohe Xiao chengde Medical University Chengde 067000China Department of Hepatology The Fifth Medical Center of PLA General HospitalBeijing 100039China China Military Institute of Chinese Materia Fifth Medical Center of Chinese PLA General HospitalBeijing 100039China National Key Laboratory of Kidney Diseases The Fifth Medical Center of PLA General HospitalBeijing 100039China School of Stomatology Heilongjiang Key Lab of Oral Biomedicine Materials and Clinical Application&Experimental Center for Stomatology EngineeringJiamusi UniversityJiamusi 154007China Southern Medical University Guangzhou 510515China

Objective:Acute lung injury(ALI)is characterized by inflammation and currently lacks an efficacious pharmacological *** medicine combination of Lonicerae Japonicae Flos(LJF)and Forsythiae Fructus(FF)demonstrates combined properties in its anti-infective,anti-inflammatory,and therapeutic effects,particularly in alleviating respiratory *** previous studies,Chinese medicine has shown promising efficacy in lipopolysaccharides(LPS)-induced ***,there have been no reports of LJF and FF pairing for lung *** aim of this study is to compare the efficacy of herb pair Lonicerae Japonicae Flos-Forsythiae Fructus(LF)with LJF or FF alone in the treatment of ALI,and to explore whether LJF and FF have a combined effect in the treatment of lung injury,along with the underlying mechanism ***:A total of 36 mice were divided into six groups(control,model,LJF,FF,LF,dexamethasone)based on the treatments they received after undergoing sham-operation/LPS tracheal instillation.H&E staining and pulmonary edema indexes were used to evaluate lung injury *** exudate cells(AECs)were counted based on cell count in bronchoalveolar lavage fluid(BALF),and neutrophil percentage in BALF was measured using flow ***(MPO)activity in BALF was measured using enzyme-linked immunosorbent assay(ELISA),while the production of IL-1.,TNF-α,and IL-6 in the lung and secretion level of them in BALF were detected by quantitative polymerase chain reaction(qPCR)and *** effect of LJF,FF,and LF on the expression of Caspase-1.and IL-1.proteins in bone marrow derived macrophages(BMDMs)supernatant was assessed using Western blot method under various inflammasome activation *** addition,the concentration of IL-1.and changes in lactatedehydrogenase(LDH)release levels in BMDMs supernatant after LJF,FF,and LF administration,respectively,were measured using ***,the effects of LJF,FF and LF on STING and IRF3 phosphor

关键词： acute lung injury BMDMS inflammasomes Lonicerae Japonicae Flos-Forsythia Fructus drug pair STING

海水腐蚀对砂岩物理力学特性与微观结构破坏特征的影响研究

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矿业科学学报 2024年第2期9卷 178-189页

作者：周韬陈昌鹏张旸旸殷雪菡陈家嵘深圳大学深地科学与绿色能源研究院深圳518060 Department of Civil Environmental Engineering University of AlbertaEdmonton AlbertaT6G1K7Canada

针对海底隧道围岩在海水腐蚀下物理力学性能劣化的问题,选取典型砂岩作为研究对象,基于深圳海底隧道所在区域海水的取样数据配制不同浓度的模拟海水溶液,对砂岩试样进行不同浓度、不同时间的腐蚀浸泡处理,再进行单轴压缩试验,并结合声... 详细信息

针对海底隧道围岩在海水腐蚀下物理力学性能劣化的问题,选取典型砂岩作为研究对象,基于深圳海底隧道所在区域海水的取样数据配制不同浓度的模拟海水溶液,对砂岩试样进行不同浓度、不同时间的腐蚀浸泡处理,再进行单轴压缩试验,并结合声发射与扫描电子显微镜(SEM)技术,对海水腐蚀损伤砂岩的物理力学特性、破坏过程和微观结构演化特征进行分析研究。结果表明:随着腐蚀溶液浓度增加,砂岩试样表面越早发生脱落且面积越大,试样密度小幅度增加;峰值应力和弹性模量均随腐蚀时间增加而降低,但二者降低幅度及速率不同;随着腐蚀时间以及浓度的增加,除高浓度组砂岩试样在腐蚀45 d之后声发射事件活跃期有所缩短外,其余组砂岩试样的声发射事件活跃期均有所增长,砂岩试样内部缺陷逐渐发育,结构逐渐疏松,蜂窝状腐蚀区域面积增大,盐离子白色结晶区域逐渐扩大。

关键词：海水腐蚀水岩作用单轴压缩声发射 SEM

CHCHD2 Thr61.le mutation impairs F1.0-ATPase assembly in in vitro and in vivo models of Parkinson's disease

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Neural Regeneration Research 2024年第1期19卷 196-204页

作者： Xiang chen Yuwan Lin Zhiling Zhang Yuting Tang Panghai Ye Wei Dai Wenlong Zhang Hanqun Liu Guoyou Peng Shuxuan Huang Jiewen Qiu Wenyuan Guo Xiaoqin Zhu {1.hua Wu Yaoyun Kuang Pingyi Xu Miaomiao Zhou Department of Neurology The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina Department of Neurology The People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxi Zhuang Autonomous RegionChina School of Basic Medical Sciences Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina Department of Neurology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghaiChina

Mitochondrial dysfunction is a significant pathological alte ration that occurs in Parkinson's disease(PD),and the Thr61.le(T61.)mutation in coiled-coil helix coiled-coil helix domain containing 2(CHCHD2),a crucial mitochondrial protein,has been reported to cause Parkinson's ***-ATPase participates in the synthesis of cellular adenosine triphosphate(ATP)and plays a central role in mitochondrial energy ***,the specific roles of wild-type(WT)CHCHD2 and T61.-mutant CHCHD2 in regulating F1.O-ATPase activity in Parkinson's disease,as well as whether CHCHD2 or CHCHD2 T61. affects mitochondrial function through regulating F1.O-ATPase activity,remain unclea ***,in this study,we expressed WT CHCHD2 and T61.-mutant CHCHD2 in an MPP^(+)-induced SH-SY5Y cell model of *** found that CHCHD2 protected mitochondria from developing MPP^(+)-induced *** normal conditions,ove rexpression of WT CHCHD2 promoted F1.O-ATPase assembly,while T61.-mutant CHCHD2 appeared to have lost the ability to regulate F1.O-ATPase *** addition,mass spectrometry and immunoprecipitation showed that there was an interaction between CHCHD2 and *** weeks after transfection with AAV-CHCHD2 T61.,we intraperitoneally injected 1.methyl-4-phenyl-1.2,3,6-tetrahydropyridine into mice to establish an animal model of chronic Parkinson's disease and found that exogenous expression of the mutant protein worsened the behavioral deficits and dopaminergic neurodegeneration seen in this *** findings suggest that WT CHCHD2 can alleviate mitochondrial dysfunction in PD by maintaining F1.0-ATPase structure and function.

关键词： ATP synthase(F1.0-ATPase) coiled-coil helix coiled-coil helix domain containing 2 dopaminergic neuron mitochondrial dysfunction neurodegeneration oligomycin sensitivity-conferring protein Parkinson's disease T61. mutation tyrosine hydroxylase