检索结果-南通市图书馆

Exploiting sound for emerging applications of extracellular vesicles

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2024年第2期17卷 462-475页

作者： zhuhao wu Hongwei Cai Chunhui Tian Zheng Ao Lei Jiang Feng Guo Department of Intelligent Systems Engineering Indiana UniversityBloomingtonIN 47405USA

Extracellular vesicles are nano-to micro-scale,membrane-bound particles released by cells into extracellular space,and act as carriers of biomarkers and therapeutics,holding promising potential in translational ***,the challenges remain in handling and detecting extracellular vesicles for disease diagnosis as well as exploring their therapeutic capability for disease ***,we review the recent engineering and technology advances by leveraging the power of sound waves to address the challenges in diagnostic and therapeutic applications of extracellular vesicles and biomimetic *** first introduce the fundamental principles of sound waves for understanding different acoustic-assisted extracellular vesicle *** discuss the acoustic-assisted diagnostic methods including the purification,manipulation,biosensing,and bioimaging of extracellular ***,we summarize the recent advances in acoustically enhanced therapeutics using extracellular vesicles and biomimetic ***,we provide perspectives into current challenges and future clinical applications of the promising extracellular vesicles and biomimetic nanovesicles powered by sound.

关键词： extracellular vesicles exosomes biomimetic nanovesicles acoustics disease diagnostics therapeutics

Biomimetic artificial islet model with vascularized microcapsule structures for durable glycemic control

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Materials Futures 2024年第3期3卷 157-167页

作者： Jingbo Li Yile Fang zhuhao wu Luoran Shang Ling Li Department of Endocrinology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjing 210009People’s Republic of China Institute of Glucose and Lipid Metabolism Southeast UniversityNanjing 210009People’s Republic of China Shanghai Xuhui Central Hospital Zhongshan-Xuhui Hospitaland the Shanghai Key Laboratory of Medical EpigeneticsThe International Co-laboratory of Medical Epigenetics and Metabolism(Ministry of Science and Technology)Institutes of Biomedical SciencesFudan UniversityShanghai 200032People’s Republic of China Department of Clinical Laboratory Nanjing Drum Tower HospitalThe Affiliated of Medical SchoolNanjing UniversityNanjing 210008People’s Republic of China

Islet transplantation is a promising strategy for diabetes mellitus treatment as it can recapitulate endogenous insulin secretion and provide long-term glycemic *** models constructed in biomaterial scaffolds that reproduce biological characteristics of native islets is a feasible option to circumvent the dilemma of donor shortage and the requirement of chronic ***,we developed bioinspired artificial microcapsule-based islet models with microvessels for glycemic control using microfluidic electrospray *** electrospray can generate uniform hydrogel microcapsules with core-shell structure for encapsulating islet *** cell-laden microcapsules enabled the efficient transportation of nutrient,oxygen,and insulin;as well as the incorporation with microvessels for prompting glucose responsiveness and molecular *** demonstrated by in vivo experiments that the blood glucose,food intake,and body weight of diabetic mouse models were alleviated,and the glucose tolerance was promoted after the engraftment of islet *** further demonstrated the improved functionality of transplanted islet model in insulin secretion,immune escape,and microcirculation using standard histological and molecular *** results indicated that the microcapsules with microvessels are promising artificial islet models and are valuable for treating diabetes.

关键词： artificial islet model microfluidics vascularization hydrogel diabetes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

小型水库调洪演算及日常管养工作研究

城市道桥与防洪 2024年第4期 151-154,I0011页

作者：李朋闫红飞吴朱昊上海市政工程设计研究总院(集团)有限公司上海市200092

以晋江市东山水库为例,根据小型水库调查测绘情况,梳理水库设施存在的问题并分析原因,提出相应的措施建议.对试运行的小型水库山围塘日常管理和养护制度进行评估分析,提出更科学合理、契合当地实际的日常管养方案,研究维修养护部分(除... 详细信息

以晋江市东山水库为例,根据小型水库调查测绘情况,梳理水库设施存在的问题并分析原因,提出相应的措施建议.对试运行的小型水库山围塘日常管理和养护制度进行评估分析,提出更科学合理、契合当地实际的日常管养方案,研究维修养护部分(除草、灭蚁、灌浆)市场化运作方案及成本核算等.其成果可为其他地区小型水库山塘调洪演算及日常管养工作提供借鉴参考.

关键词：小型水库调洪演算日常管理管养分离

Replacing alkyl side chain of non-fullerene acceptor with siloxane-terminated side chain enables lower surface energy towards optimizing bulk-heterojunction morphology and high photovoltaic performance

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2021年第7期64卷 1208-1218页

作者： Rihang Qiu zhuhao wu Suhan Li Haiying Jiang Qian Wang Yinchu Chen Xuanchen Liu Lianjie Zhang Junwu Chen State Key Laboratory of Luminescent Materials&Devices Institute of Polymer Optoelectronic Materials&DevicesGuangzhou 510640China South China University of Technology South China Institute of Collaborative InnovationDongguan 523808China

Towards a good control of the morphology of bulk-heterojunction(BHJ)active layers for polymer solar cells(PSCs),selecting an appropriate side chain for a polymer donor and a nonfullerene acceptor(NFA)is very *** this work,two novel NFAs i-IE-4F and i-IESi-4 F comprising alkyl and siloxane-terminated side chains on the central indacenodithiophene(IDT)core,respectively,were *** the siloxane-terminated side chain to i-IESi-4 F affords surface energy(γ)of33.32 mN/m,much lower than that of 39.83 mN/m for i-IE-4F,supplying a big chance to tune miscibility with a polymer *** fluorobenzotriazole-based polymer donors J52 and PBZ-2Si bearing alkyl and siloxane-terminated side chains,respectively,showγvalues of 36.08 and 33.10 mN/m,*** estimated Flory-Huggins interaction parameters(χD,A)indicate that the i-IESi-4 F is more miscible than i-IE-4F in pairing with J52 or *** resulting i-IESi-4 F-based blend film exhibits low film roughness and accompanies obviously improved BHJ *** PSCs,the J52:i-IESi-4F and PBZ-2Si:i-IESi-4 F active layers display power conversion efficiencies(PCEs)of 12.67%and 14.54%,respectively,all remarkably higher than PCEs≤7.34%of the i-IE-4F-based active ***,the PBZ-2Si:i-IESi-4 F active layer,a donor:acceptor blend system comprising siloxane-terminated side chains(DSi:ASimatching)with the highest BHJ miscibility due to the combinatory effect of the side chains,shows the highest efficiency,as supported by efficient exciton dissociation,the lowest bimolecular recombination,and the optimal charge *** results demonstrate that attaching siloxane-terminated side chains to NFAs,as a side chain engineering,has big potential in lowering their surface energy towards fine control of BHJ morphology and leading to a better donor:acceptor blend system.

关键词： terminated acceptor donor

Acoustofluidics for cell patterning and tissue engineering

在线全文

学校读者我要写书评

暂无评论

Engineered Regeneration 2022年第4期3卷 397-406页

作者： zhuhao wu Meidie Pan Jinglin Wang Baojie Wen Ling Lu Haozhen Ren Institute of Translational Medicine The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing210002China School of Medicine Zhejiang UniversityHangzhou310030China

Acoustofluidics has been a promising approach using sound waves to manipulate particles and actuate fluids in biomedical *** usually generates acoustic radiation force and acoustic streaming to initiate diffraction,reflection and interference,building up a pressure distribution to facilitate accurate manipulation of micro-or nano-scale particles and *** to its remarkable contact-free and biocompatible advantages,acoustoflu-idics has been used in high-throughput cell analysis,size-controllable organoid structures,and functional tissue *** enumerate the basic concepts and the sufficient research of acoustofluidics in precise patterning and tissue engineering in this review,including the design and function of four typical acoustofluidic devices,var-ious forms of cell patterning and 3D tissue ***,we outlined current challenges and future directions of acoustofluidics in biomedicine and tissue engineering.

关键词： Acoustofluidics Cell patterning Tissue engineering Disease models Engineered regeneration

Engineering human spinal microphysiological systems to model opioid-induced tolerance

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第4期22卷 482-490页

作者： Hongwei Cai Zheng Ao Chunhui Tian zhuhao wu Connor Kaurich Zi Chen Mingxia Gu Andrea G.Hohmann Ken Mackie Feng Guo Department of Intelligent Systems Engineering Indiana UniversityBloomingtonIN47405United States Department of Surgery Brigham and Women’s Hospital/Harvard Medical SchoolBostonMA02115United States Division of Pulmonary Biology Center for Stem Cell and Organoid Medicine(CuSTOM)Cincinnati Children’s Hospital Medical CenterCincinnatiOH45229United States University of Cincinnati School of Medicine CincinnatiOH45229United States Gill Center for Biomolecular Science and Department of Psychological and Brain SciencesIndiana UniversityBloomingtonIN47405United States

pioids are commonly used for treating chronic ***,with continued use,they may induce tolerance and/or hyperalgesia,which limits therapeutic *** human mechanisms of opioid-induced tolerance and hyperalgesia are significantly understudied,in part,because current models cannot fully recapitulate human ***,we engineered novel human spinal microphysiological systems(MPSs)integrated with plug-and-play neural activity sensing for modeling human nociception and opioid-induced *** spinal MPS consists of a flattened human spinal cord organoid derived from human stem cells and a 3D printed organoid holder device for plug-and-play neural activity *** found that the flattened organoid design of MPSs not only reduces hypoxia and necrosis in the organoids,but also promotes their neuron maturation,neural activity,and functional *** further demonstrated that prolonged opioid exposure resulted in neurochemical correlates of opioid tolerance and hyperalgesia,as measured by altered neural activity,and downregulation ofμ-opioid receptor expression of human spinal *** MPSs are scalable,cost-effective,easy-to-use,and compatible with commonly-used well-plates,thus allowing plug-and-play measurements of neural *** believe the MPSs hold a promising translational potential for studying human pain etiology,screening new treatments,and validating novel therapeutics for human pain medicine.

关键词： Microphysiological systems Organ-on-chip In-situ electrical sensing Spinal cord organoids Opioid-induced tolerance and hyperalgesia Neural activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

谐波减速器刚轮用材质摩擦磨损性能分析

金属热处理 2024年第1期49卷 47-52页

作者：邵洙浩杨大伟吴咏锦刘钊赵亮北京航空材料研究院北京100095 北京科技大学材料科学与工程学院北京100083 北京中技克美谐波传动股份有限公司北京101318

通过对合金成分、显微组织和耐磨性的定量分析,对比了两种球墨铸铁(QT1和QT2)和40Cr钢的刚轮摩擦磨损性能。结果表明,QT1和QT2基体组织分别为珠光体、针状铁素体和残留奥氏体,两者石墨球尺寸细小且圆整度较高。QT1和QT2球墨铸铁的磨损... 详细信息

通过对合金成分、显微组织和耐磨性的定量分析,对比了两种球墨铸铁(QT1和QT2)和40Cr钢的刚轮摩擦磨损性能。结果表明,QT1和QT2基体组织分别为珠光体、针状铁素体和残留奥氏体,两者石墨球尺寸细小且圆整度较高。QT1和QT2球墨铸铁的磨损位置较为集中,起伏波动小,最大磨痕深度分别为3.7μm和2.6μm,而40Cr钢多为深浅不一的犁沟,起伏波动大,最大磨痕深度为7.6μm。与40Cr钢相比,两种球墨铸铁具有良好的耐磨性和更低的摩擦因数,国内谐波减速器企业可采用球墨铸铁作为刚轮材料代替40Cr钢。

关键词：刚轮球墨铸铁 40Cr钢耐磨性能

右美托咪定对口腔鳞癌细胞增殖、迁移和侵袭的影响

在线全文

学校读者我要写书评

暂无评论

临床麻醉学杂志 2023年第6期39卷 623-630页

作者：吴朱昊张晓昕泥艳红孙国文董迎春南京大学医学院附属口腔医院、南京市口腔医院口腔麻醉科 210008 南京大学医学院附属口腔医院、南京市口腔医院中心实验室 210008 南京大学医学院附属口腔医院、南京市口腔医院口腔颌面外科 210008

目的探讨右美托咪定对口腔鳞癌细胞增殖、迁移和侵袭的影响及机制。方法实验一:选用人口腔鳞癌细胞系HN6和CAL27,根据右美托咪定浓度不同随机均分为六组:阴性对照组(NC组)、右美托咪定1 nmol/L组(D1组)、右美托咪定10 nmol/L组(D2组)、... 详细信息

目的探讨右美托咪定对口腔鳞癌细胞增殖、迁移和侵袭的影响及机制。方法实验一:选用人口腔鳞癌细胞系HN6和CAL27,根据右美托咪定浓度不同随机均分为六组:阴性对照组(NC组)、右美托咪定1 nmol/L组(D1组)、右美托咪定10 nmol/L组(D2组)、右美托咪定100 nmol/L组(D3组)、右美托咪定1μmol/L组(D4组)和右美托咪定10μmol/L组(D5组)。培养24、48 h后分别采用CCK-8法、划痕实验和Transwell实验检测细胞增殖、迁移和侵袭能力。实验二:利用转录组测序技术筛选右美托咪定1μmol/L处理HN6细胞12 h后差异表达的基因。采用慢病毒转染细胞,建立稳定敲低细胞色素P4501B1(CYP1B1)的HN6细胞株。将细胞随机分为四组:shCtrl组(C组)、shCYP1B1组(CYP组)、shCtrl+右美托咪定组(CD组)和shCYP1B1+右美托咪定组(CYPD组)。采用划痕实验和Transwell实验检测HN6细胞迁移和侵袭能力。结果实验一:与NC组比较,D2组、D3组、D4组和D5组HN6和CAL27细胞迁移率均明显升高,基质胶内穿膜细胞数均明显增多;D1组HN6细胞迁移率明显升高(P<0.05)。与D1组比较,D2组、D3组、D4组和D5组HN6和CAL27细胞迁移率均明显升高,基质胶内穿膜细胞数均明显增多(P<0.05)。与D2组比较,D3组、D4组和D5组HN6和CAL27细胞迁移率均明显升高,基质胶内穿膜细胞数均明显增多(P<0.05)。与D3组比较,D4组HN6、CAL27细胞和D5组HN6细胞迁移率明显升高,基质胶内穿膜细胞数均明显增多(P<0.05)。与D4组比较,D5组HN6细胞迁移率明显升高,基质胶内穿膜细胞数明显增多;D5组CAL27细胞迁移率明显降低,基质胶内穿膜细胞数明显减少(P<0.05)。培养24、48 h后六组HN6和CAL27细胞存活率差异均无统计学意义。实验二:对右美托咪定处理后的HN6细胞进行转录组测序,筛选出54个差异表达基因,其中CYP1B1表达明显上调。慢病毒成功转染HN6细胞,构建稳定敲低CYP1B1的HN6细胞株。与C组比较,CYP组迁移率明显降低,基质胶内穿膜细胞数明显减少;CD组和CYPD组迁移率明显升高,基质胶内穿膜细胞数明显增多(P<0.05)。与CYP组比较,CD组和CYPD组迁移率明显升高,基质胶内穿膜细胞数增多(P<0.05)。与CD组比较,CYPD组迁移率明显降低,基质胶内穿膜细胞数明显减少(P<0.05)。结论右美托咪定能增强口腔鳞癌细胞迁移能力和侵袭能力,该作用可能与右美托咪定处理后CYP1B1表达相关。

关键词：细胞色素P4501B1 右美托咪定口腔鳞癌增殖迁移侵袭

SWMM模型在海绵城市建设径流控制模拟中的应用

在线全文

学校读者我要写书评

暂无评论

城市道桥与防洪 2019年第11期 69-72,80,m0010页

作者：李朋贺佳吴朱昊唐仕勤上海市政工程设计研究总院(集团)有限公司上海市200092 上海市堤防(泵闸)设施管理处上海市200080

针对海绵城市建设径流控制需求,利用SWMM模型模拟海绵城市建设措施在径流控制中的作用,分析不同控制单元的出水量、峰值流量和峰现时间,评估低影响开发措施布置下的片区径流控制效果。结果显示,低影响开发措施布置有效缓解雨水管网排水... 详细信息

针对海绵城市建设径流控制需求,利用SWMM模型模拟海绵城市建设措施在径流控制中的作用,分析不同控制单元的出水量、峰值流量和峰现时间,评估低影响开发措施布置下的片区径流控制效果。结果显示,低影响开发措施布置有效缓解雨水管网排水压力,多数出水口出水量小于对应调蓄湿塘容积,实现径流控制要求。各控制单元峰值流量削减比例在82.15%-88.63%,峰现时间延长6~17min,有利于研究区雨洪资源回用,补充地下水并减小区域洪涝风险。

关键词：海绵城市低影响开发水文模拟 SWMM模型