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Transgene directionally integrated into C-genome of Brassica napus

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Chinese Science Bulletin 2006年第13期51卷 1578-1585页

作者： LI Jun FANG Xiaoping WANG zhuan LUO Lixia hu Qiong Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture Institute of Oil Crops Research Chinese Academy of Agricultural Sciences Wuhan 430062 China

Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus To obtain C-genome transgenic B. napus, herbi- cide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the trans- genic B. oleracea as paternal plants and 8 non- transgenic varieties of B. rapa as maternal plants, C- genome transgenic B. napus with bar gene was arti- ficially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resyn- thesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray con- firmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines.

关键词： C-染色体组 Bar基因定向转基因芸苔再合成

Sn-MOFs/G composite materials for long cycling performance lithium ion batteries

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Rare Metals 2022年第5期41卷 1504-1511页

作者： Jian-Guo Zhao Hong-Yan Zhou zhuan hu Ya-Wen Wu Hong Jia Xian-Ming Liu School of Physics and Electronic Information Luoyang Normal UniversityLuoyang 471022China Henan Key Laboratory of Electromagnetic Transformation and Detection Luoyang Normal UniversityLuoyang 471022China School of Physics South China University of TechnologyGuangzhou 510640China School of Physics School of Physics and ElectronicsHunan UniversityChangsha 410082China School of Chemistry and Chemical Engineering Luoyang Normal UniversityLuoyang 471022China

Metal-organic frameworks(MOFs)are considered as one of the most important anode materials because of their controllable preparation,large specific surface area and controllable ***,low electronic conductivity causes u... 详细信息

关键词： lithium capacity cycling

The Effectiveness of Antiviral Treatment in Severe COVID-19 Patients in Wuhan,China:A Multicenter Study

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Biomedical and Environmental Sciences 2022年第1期35卷 58-63页

作者： ZHOU Xian Long DING Guo Yong YANG Lu Yu LIU Rui Ning HOU Hai Feng WANG Ping MA Min hu zhuan zhuan huANG Lei XU Xi Zhu hu Quan ZHAO Yan XING Wei Jia ZHAO Zhi Gang Emergency Center Zhongnan Hospital of Wuhan UniversityWuhan 430071HubeiChina School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesTai’an 271016ShandongChina Intensive Care Unit Wuhan Third HospitalWuhan 430060HubeiChina Intensive Care Unit Huazhong University of Science and Technology Union Jiangbei HospitalWuhan 430100HubeiChina Intensive Care Unit The first people's Hospital of Jiangxia DistrictWuhan 430200HubeiChina hubei Clinical Research Center for Emergency and Resuscitation Zhongnan Hospital of Wuhan UniversityWuhan 430071HubeiChina

Coronavirus disease 2019(COVID-19)has inarguably caused the most challenging *** less than 2 years,greater than 200 million cases of COVID-19 and 4.5 million deaths have been reported worldwide[1].The treatment strategy of an emerging infectious disease is a huge challenge for clinical practitioners because of missing key knowledge about the disease.

关键词： Patients clinical COV

Number and Distribution of Fiducial Fibers in a Spectroscopic Survey Telescope

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Research in Astronomy and Astrophysics 2022年第2期22卷 108-117页

作者： Shi-Peng Duan Zeng-Xiang Zhou Jia-Le Zuo Meng-Tao Li Zhi-Gang Liu Hong-zhuan hu Jian-Ping Wang Xiang-Qun Cui Yong Zhang Hao-Tong Zhang Department of Precision Machinery and Precision Instrumentation University of Science and Technology of ChinaHefei 230026China National Astronomical Observatories/Nanjing Institute of Astronomical Optics&Technology Chinese Academy of ScienceNanjing 210042China CAS Key Laboratory of Astronomical Optics&Technology Nanjing Institute of Astronomical Optics&TechnologyNanjing 210042China National Astronomical Observatories Chinese Academy of SciencesBeijing 100012China

To date,the Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST)has been in operation for 12 *** improve the telescope's astronomical observation accuracy,the original open-loop fiber positioning system of LAMOST is in urgent need of *** upgrade plan is to install several fiber view cameras(FVCs)around primary mirror B to build a closed-loop feedback control *** FVCs are 20 m from the focal *** reduce a series of errors when the cameras detect the positions of the optical fibers,we designed fiducial fibers on the focal surface to be fiducial points for the *** the number of fiducial fibers can improve the detection accuracy of the FVC system,but it will also certainly reduce the number of fiber positioners that can be used for ***,the focus of this paper is how to achieve the quantity and distribution that meet the requirements of system *** this paper,we introduce the necessity of using fiducial fibers,propose a method for selecting their number and present several methods for assessing the uniformity of their ***,we implement particle swarm optimization to find the best distribution of fiducial fibers.

关键词： instrumentation detectors-techniques image processing-methods data analysis-telescopes

Immunolocalization of secretion complex in Streptococcus mutans

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Journal of Microbiology and Immunology 2007年第1期5卷 13-16页

作者： PING hu zhuan BIAN MING WEN FAN MIN NIE Key Laboratory for Oral Biomedical Engineering of Ministry of Education School and Hospital of Stomatology Wuhan University P. R. China

Each secretion （See） machinery minimally consists of three integral inner membrane pro- teins SecYEG. In order to know the distribution pattern of the Sec complex in Streptococcus mutans, we explored the subcellular localization of SecY. The anti-secY antibody was examined on Western blots to evaluate specificity. An indirect post-embedding immunogold method was used to determine the subcellular localization of the SecY in the cytoplasmic membrane of the Streptococcus mutans GS-5. lmmunoblotting experiments showed that the anti-seeY antibody specifically recognized a single band of about 47.8 kDa. Immunogold electron microscopy image of section with anti-secY antibody revealed a single intense focus of gold particles at a discrete location on the cytoplasmic membrane of the Streptococcus mutans GS-5. SecY clustered to an asymmetric microdomain, which indicated for the first time that See complex presented a unisite on the cytoplasmic membrane of Streptococcus mutans.

关键词： Streptococcus mutans Secretion complex SecY

Mode coupling with Fabry-Perot modes in photonic crystal slabs

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Chinese Physics B 2024年第8期33卷 90-94页

作者：秦恳胡鹏刘杰向红韩德专 Department of Physics and Chongqing Key Laboratory for Strongly Coupled Physics Chongqing UniversityChongqing 401331China School of Science Chongqing Key Laboratory of New Energy Storage Materials and DevicesChongqing University of TechnologyChongqing 400054China

Fabry–Perot(FP)modes are a class of fundamental resonances in photonic crystal(PhC)*** to their low quality factors,FP modes are frequently considered as background fields with their resonance nature being ***,FP modes can play important roles in some phenomena,as exemplified by their coupling with guided resonance(GR)modes to achieve bound states in the continuum(BIC).Here,we further demonstrate the genuine resonance mode capability of FP modes PhC ***,we utilize temporal coupled-mode theory to obtain the transmittance of a PhC slab based on the FP ***,we construct exceptional points(EPs)in both momentum and parameter spaces through the coupling of FP and GR ***,we identify a Fermi arc connecting two EPs and discuss the far-field polarization *** work elucidates that the widespread FPs in PhC slabs can serve as genuine resonant modes,facilitating the realization of desired functionalities through mode coupling.

关键词： Fabry–Perot mode photonic crystal slab mode coupling exceptional point

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氟硅酸制备高纯硅副产氟化盐的工艺研究

河南化工 2020年第6期37卷 23-25页

作者：胡专多氟多化工股份有限公司河南焦作454006

概述了国内外高纯硅烷的研究进展,采用氟硅酸制备高纯硅副产氟化盐,分析了实验原料、实验方法、工艺流程、工艺关键点和创新点,对比了该工艺制备高纯硅烷和国内外超大规模集成电路VLSI用硅烷的指标。该技术创新点较多,经济效益、环境效... 详细信息

概述了国内外高纯硅烷的研究进展,采用氟硅酸制备高纯硅副产氟化盐,分析了实验原料、实验方法、工艺流程、工艺关键点和创新点,对比了该工艺制备高纯硅烷和国内外超大规模集成电路VLSI用硅烷的指标。该技术创新点较多,经济效益、环境效益和社会效益都极其显著,实现了氟硅资源的高效利用。

关键词：氟硅酸高纯硅氟化盐

尿石素A通过调控有氧糖酵解抑制肝癌细胞生长的机制

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中国现代应用药学 2024年第8期41卷 1047-1055页

作者：胡红柳何志龙王专蒋利和右江民族医学院基础医学院广西百色533000 湖北民族大学生物资源保护与利用湖北省重点实验室湖北恩施445000 广西大学轻工与食品工程学院南宁530004 厦门大学福建省药物新靶点研究重点实验室福建厦门361005

目的探讨尿石素抑制肝癌细胞生长的分子机制。方法不同浓度尿石素A(urolithin A,Uro-A)处理肝癌huh-7细胞,CCK-8法检测细胞抑制率,并计算IC50,克隆形成试验检测细胞增殖能力,细胞划痕试验评估细胞迁移率,比色法检测细胞培养液中葡萄... 详细信息

目的探讨尿石素抑制肝癌细胞生长的分子机制。方法不同浓度尿石素A(urolithin A,Uro-A)处理肝癌huh-7细胞,CCK-8法检测细胞抑制率,并计算IC50,克隆形成试验检测细胞增殖能力,细胞划痕试验评估细胞迁移率,比色法检测细胞培养液中葡萄糖摄取量和乳酸水平,化学发光法检测细胞内ATP含量;Western blotting检测各浓度Uro-A对葡萄糖转运载体GLUT1、糖酵解关键酶(HK2、PKM2、LDHA)、p53、p-p38和Bcl-2蛋白表达的影响,流式细胞术和TUNEL法检测细胞凋亡率。结果 CCK-8检测结果显示,Uro-A能明显抑制huh-7细胞的增殖,IC50为(48.54±1.21)μmol·L^(-1)。Uro-A处理后细胞克隆形成量减少,迁移率降低。不同浓度Uro-A作用后细胞葡萄糖摄取量、乳酸水平和ATP生成减少,糖酵解相关蛋白GLUT1、PKM2、LDHA和HK2表达量下降;Western blotting显示p53和pp38被激活,Bcl-2表达降低;流式细胞术和TUNEL法显示细胞凋亡率显著升高。结论 Uro-A可抑制huh-7细胞增殖和迁移,其机制可能是通过p53、p-p38和Bcl-2抑制糖酵解,使细胞生长受阻,诱导细胞凋亡。

关键词：尿石素肝癌糖酵解细胞生长

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST)

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Research in Astronomy and Astrophysics 2012年第9期12卷 1197-1242页

作者： Xiang-Qun Cui Yong-Heng Zhao Yao-Quan Chu Guo-Ping Li Qi Li Li-Ping Zhang Hong-Jun Su Zheng-Qiu Yao Ya-Nan Wang Xiao-Zheng Xing Xin-Nan Li Yong-Tian Zhu Gang Wang Bo-Zhong Gu A-Li Luo Xin-Qi Xu Zhen-Chao Zhang Gen-Rong Liu Hao-Tong Zhang De-hua Yang Shu-Yun Cao Hai-Yuan Chen Jian-Jun Chen Kun-Xin Chen Ying Chen Jia-Ru Chu Lei Feng Xue-Fei Gong Yong-hui Hou Hong-zhuan hu Ning-Sheng hu Zhong-Wen hu Lei Jia Fang-hua Jiang Xiang Jiang Zi-Bo Jiang Ge Jin Ai-hua Li Yan Li Ye-Ping Li Guan-QunLiu Zhi-Gang Liu huo-Ming Shi Zheng-Hong Tang Qing-Sheng Tao Xiang-Yan Yuan Chao Zhai Jing Zhang Yan-Xia Zhang Yong Zhang Ming Zhao Fang Zhou Guo-hua Zhou Jie Zhu Si-Cheng Zou National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China yzhao @ *** Nanjing Institute of Astronomical Optics and Technology National Astronomical ObservatoriesChinese Academy of Sciences Nanjing 210042 China University of Science and Tecfinologyof China Hefei 230026 China Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China Institute of Architecture Design and Research Chinese Academy of Sciences Beijing 100190China

The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST, also called the Guo Shou Jing Telescope) is a special reflecting Schmidt telescope. LAMOST’s special design allows both a large aperture (effective aperture of 3.6 m–4.9 m) and a wide field of view (FOV) (5°). It has an innovative active reflecting Schmidt configuration which continuously changes the mirror’s surface that adjusts during the observation process and combines thin deformable mirror active optics with segmented active optics. Its primary mirror (6.67m×6.05 m) and active Schmidt mirror (5.74m×4.40 m) are both segmented, and composed of 37 and 24 hexagonal sub-mirrors respectively. By using a parallel controllable fiber positioning technique, the focal surface of 1.75 m in diameter can accommodate 4000 optical fibers. Also, LAMOST has 16 spectrographs with 32 CCD cameras. LAMOST will be the telescope with the highest rate of spectral acquisition. As a national large scientific project, the LAMOST project was formally proposed in 1996, and approved by the Chinese government in 1997. The construction started in 2001, was completed in 2008 and passed the official acceptance in June 2009. The LAMOST pilot survey was started in October 2011 and the spectroscopic survey will launch in September 2012. Up to now, LAMOST has released more than 480 000 spectra of objects. LAMOST will make an important contribution to the study of the large-scale structure of the Universe, structure and evolution of the Galaxy, and cross-identification of multiwaveband properties in celestial objects.

关键词： techniques: wide field telescope - active optics - multi fiber - spectroscopy survey - data reduction