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Journal of Integrative Agriculture 2017年第1期16卷 16-26页

作者： WANG Zhen CHEN Jun-yu ZHU Yu-jun FAN Ye-yang zhuang jie-yun State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research Institute Hangzhou 310006 P.R. China

Grain size is a major determinant of grain weight and a trait having important impact on grain quality in rice. The objective of this study is to detect QTLs for grain size in rice and identify important QTLs that have not been well characterized before. The QTL mapping was first performed using three recombinant inbred line populations derived from indica rice crosses Teqing/IRBB lines, Zhenshan 97/Milyang 46, Xieqingzao/Milyang 46. Fourteen QTLs for grain length and 10 QTLs for grain width were detected, including seven shared by two populations and 17 found in one population. Three of the seven com- mon QTLs were found to coincide in position with those that have been cloned and the four others remained to be clarified. One of them, qGSIO located in the interval RM6100-RM228 on the long arm of chromosome 10, was validated using F2：3 populations and near isogenic lines derived from residual heterozygotes for the interval RM6100-RM228. The QTL was found to have a considerable effect on grain size and grain weight, and a small effect on grain number. This region was also previously detected for quality traits in rice in a number of studies, providing a good candidate for functional analysis and breeding utilization.

关键词： grain size quantitative trait locus residual heterozygote rice (Oryza sativa L.)

Quantitative Trait Loci for Heading Date and Their Relationship with Genetic Control of Yield Traits in Rice (Oryza sativa)

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Rice science 2013年第1期20卷 1-12页

作者： GUO Liang ZHANG Zhen-hua zhuang jie-yun Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research Institute

Grain yield and heading date are key factors determining the commercial potential of a rice variety. Mapping of quantitative trait loci （QTLs） in rice has been advanced from primary mapping to gene cloning, and heading date and yield traits have always attracted the greatest attention. In this review, genomic distribution of QTLs for heading date detected in populations derived from intra-specific crosses of Asian cultivated rice （Oryza sativa） was summarized, and their relationship with the genetic control of yield traits was analyzed. The information could be useful in the identification of QTLs for heading date and yield traits that are promising for the improvement of rice varieties.

关键词： rice heading date yield traits quantitative trait locus gene

Quantitative Trait Loci for Panicle Size and Grain Yield Detected in Interval RM111-RM19 784 on the Short Arm of Rice Chromosome 6

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Agricultural Sciences in China 2010年第8期9卷 1085-1092页

作者： GONG Jun-yi DU Jing-hong FAN Ye-yang WU Ji-rong zhuang jie-yun Chinese National Center for Rice Improvement and State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 PR. China College of Agricultural Resources and Environmental Sciences Heilongjiang University Harbin 150080 P.R.China

A rice residual heterozygous line （RHL） carrying a heterozygous segment extending from RM111 to RM19784 on the short arm of rice chromosome 6 was selected from a RHL-derived population used previously. The resultant F2：3 population was used to detect quantitative trait loci （QTLs） for three yield traits, the number of spikelets per panicle （NSP）, the number of grains per panicle （NGP） and grain yield per plant （GY）. Two QTLs for NSP, one QTL for NGP and one QTL for GY were detected, all of which were partially dominant and had the enhancing alleles from the maternal line Zhenshan 97B. Analysis based on the genotypic groups of the markers closely linked to the two QTLs for NSP indicated that they did not interact with each other. Two F2 populations and two near isogenic line （NIL） sets segregating in two sub-regions of interval RM111-RM19784 were developed. The two QTLs for NSP were validated, of which one had major effect and was co-segregated with heading date gene Hdl, and the other had smaller effect and was located in an upper region linked to Hdl. The two regions also showed significant effects on the number of filled grain and grain yield, although the effect on the number of filled grain was less consistent.

关键词： rice (Oryza sativa L.) short arm of chromosome 6 residual heterozygous line quantitative trait locus yield

Dissection of QTLs for Yield Traits on the Short Arm of Rice Chromosome 6

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Agricultural Sciences in China 2008年第5期7卷 513-520页

作者： DU Jing-hong FAN Ye-yang WU Ji-rong zhuang jie-yun Chinese National Center for Rice Improvement/State Key Laboratory of Rice Science China National Rice Research Institute Hangzhou310006 P.R.China

This study was undertaken to dissect quantitative trait loci （QTLs） controlling yield traits on the short arm of rice chromosome 6. A residual heterozygous line that carries a heterozygous segment extending from RM587 to RM19784 on the short arm of rice chromosome 6 was selected from an F7 population of the indica rice cross Zhenshan 97B/Milyang 46. An F2：3 population consisting of 221 lines was derived and grown in two trial sites. Six yield traits including number of panicles per plant, number of filled grains per panicle, total number of spikelets per panicle, spikelet fertility, 1 000-grain weight, and grain yield per plant were measured. An SSR marker linkage map was constructed and employed to determine QTLs for yield traits with Windows QTL Cartographer 2.5. QTLs were detected in the target interval for all the traits analyzed except NP, with phenotypic variance explained by a single QTL ranging between 6.3% and 35.2%. Most of the QTLs for yield components acted as additive QTLs, while the three QTLs for grain yield had dominance degrees of 1.65, 0.84, and -0.42, respectively. It was indicated that three or more QTLs for yield traits were located in the target region. The genetic action mode, the direction of the QTL effect, and the magnitude of the QTL effect varied among different QTLs for a given trait, and among QTLs for different traits that were located in the same interval.

关键词： yield traits residual heterozygous line quantitative trait locus short arm of chromosome 6 rice (Oryza sativa L.)

Mapping of QTLs for Leaf Malondialdehyde Content Associated with Stress Tolerance in Rice

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Rice science 2009年第1期16卷 72-74页

作者： JIANG Jing zhuang jie-yun FAN Ye-yang SHEN Bo College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310012 China Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China

Malondialdehyde （MDA） is the final product of lipid peroxidation, and MDA content can reflect the stress tolerance of plants. To map QTLs conditioning the MDA content in rice leaves, a recombinant inbred line （RIL） population with 247 lines derived from an indica-indica cross Zhenshan 97BxMilyang 46, and a linkage map consisting of 207 DNA markers were used. The RIL population showed a transgressive segregation in the MDA content of rice leaves. Two QTLs for the MDA content in rice leaves were detected in the intervals RG532-RG811 and RG381-RG236 on chromosome 1, with the additive effects from maternal and paternal parents, accounting for 4.33% and 4.62% of phenotype variations, respectively.

关键词： rice malondialdehyde content quantitative trait locus leaf stress tolerance

Quantitative Trait Loci for Grain Chalkiness and Endosperm Transparency Detected in Three Recombinant Inbred Line Populations of Indica Rice

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Journal of Integrative Agriculture 2013年第1期12卷 1-11页

作者： MEI De-yong ZHU Yu-jun YU Yong-hong FAN Ye-yang HUANG De-run zhuang jie-yun China National Rice Research Institute

Quantitative trait loci（QTL） for percentage of chalky grain,degree of chalkiness,and endosperm transparency were detected using 3 recombinant inbred line populations derived from crosses between parental lines of commercial three-line hybrids of indica *** of the populations showed great variations on heading date,and the other had a short range of heading date variation.A total of 40 QTLs were detected and fell into 15 regions of 10 chromosomes,of which 5 regions were detected for 1 or more same traits over different populations,2 were detected for different traits in different populations,3 were detected for 2 or all the 3 traits in a single population,and 5 were detected for a single trait in a single *** of these QTLs have been reported previously,but a region located on the long arm of chromosome 10 showing significant effects in all the 3 populations has not been reported *** was shown that a number of gene cloned,including the Wx and Alk for the physiochemical property of rice grain,and GW2,GS3 and GW5 for grain weight and grain size,could have played important roles for the genetic control of grain chalkiness in rice,but there are many more QTLs exerting stable effects for rice chalkiness over different genetic *** is worth paying more attentions to these regions which harbor QTL such as the qPCG5.2/qDC5.2/qET5.2 and qPCG10/qDC10/qET10 detected in our *** results also showed that the use of segregating populations having high-uniform heading date could greatly increase the efficiency of the identification of QTL responsible for traits that are subjected to great environmental influence.

关键词： percentage of chalky grain degree of chalkiness endosperm transparency quantitative trait locus headingdate variation indica rice(Oryza sativa L.subsp.indica)

Dissection of QTLs for Yield Traits Using Near Isogenic Lines Derived from Residual Heterozygous Lines in Rice

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Rice science 2008年第4期15卷 259-266页

作者： Jing-hong DU Ye-yang FAN Lei WANG jie-yun zhuang China National Rice Research Institute Hangzhou 310006 China

Three residual heterozygous lines (RHLs) carrying heterozygous segments in the intervals RM587–RM225, RM204–RM6119 and RM6119–RM402 on the short arm of rice chromosome 6, respectively, were selected from a rice population derived from an RHL for the interval RM587–RM402. Ten maternal homozygotes, 10 paternal homozygotes and 20 heterozygotes were selected from each of the F2 populations derived from the three RHLs. The three sets of near isogenic lines (NILs) were grown to detect the grain yield per plant, number of panicles per plant, number of filled grains per panicle and 1000-grain weight. With analysis on the phenotypic differences among the three genotype groups in each NIL set and those among overlapping chromosome segment substitution lines, three QTLs for number of filled grains per panicle and two QTLs for grain yield per plant were resolved. They were located in the intervals ranging from 0.66 Mb to 2.49 Mb. The additive effect was higher than the dominance effect at each locus. The allele for increasing the trait value was derived from the paternal parent at qNFGP6-1, and from the maternal alleles at other QTLs. Based on the present study, an approach for constructing new genetic resource to facilitate fine mapping of QTLs in rice was proposed.

关键词： residual heterozygous line near isogenic line quantitative trait locus yield traits rice (Oryza sativa)

Mapping of qTGW1.1,a Quantitative Trait Locus for 1000-Grain Weight in Rice (Oryza sativa L.)

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Rice science 2015年第1期22卷 9-15页

作者： ZHANG Hong-wei CHEN Yu-yu CHEN Jun-yu ZHU Yu-jun HUANG De-run FAN Ye-yang zhuang jie-yun State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research Institute

1000-grain weight （ TGW） is one ot the three component traits ot the grain yiela in rice （Oryza sativa L）. This study was conducted to validate and fine-map qTGWl. 1, a minor QTL for TGW which was previously located in a 3.7-Mb region on the long arm of rice chromosome 1. Five sets of near isogenic lines （NILs） were developed from two BC2F4 populations of the indica rice cross Zhenshan 973/Milyang 46 The NIL sets consisted of two homozygous genotypic groups differing in the regions RM11448-RM11522, RM11448-RM11549, RM1232-RM11615, RM11543-RM11554 and RM11569-RM11621, respectively. Four traits, including TGW, grain length, grain width and heading date, were measured. Phenotypic difference between the two genotypic groups in each NIL population was analyzed using SAS procedure GLM. Significant QTL effects were detected on TGW with the Zhenshan 97 allele increasing grain weight by 0.12 g to 0.14 g and explaining 8.30% to 15.19% of the phenotypic variance. Significant effects were also observed for grain length and width, whereas no significant effect was found for heading date. Based on comparison among the five NILs on the segregating regions and the results of QTL analysis, qTGWl. 1 was delimited to a 376.9-kb region flanked by DNA markers Wn28382 and RMl1554. Our results indicate that the effects of minor QTLs could be steadily detected in a highly isogenic background and suggest that such QTLs could be utilized in the breeding of high-yielding rice varieties.

关键词： 1000-grain weight minor effect quantitative trait locus rice (Oryza sativa L.)

Construction and Testing of a Primary Microsatellite Database of Major Rice Varieties in China

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Rice science 2007年第4期14卷 247-255页

作者： YING jie-zheng SHI Yong-feng E Zhi-guo ZENG Rui-zhen CHEN jie ZHU Zhi-wei zhuang jie-yun China National Rice Research Institute Hangzhou 310006 China Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China International Rice Research Institute DAPO 7777 Metro Manila Philippines

Sixty-three major inbred varieties and parental lines of major F1 hybrids used in the commercial rice production in China were assayed with rice microsatellites screened in a previous study and additional microsatellites on four chromosomes. A set of 24 markers was selected and proposed for its application in the variety identification of rice, which are distributed on all the 12 rice chromosomes with 2 markers on each chromosome. The 63 major varieties and parental lines, as well as 41 major F1 hybrids, were genotyped with the markers. Alleles detected in each line at each marker locus were verified. By matching marker genotypes of corresponding F1, maternal and paternal lines of hybrid rice, high reliability of the maternal lines was verified, data on the paternal lines were modified, and a false hybrid was removed. A database containing genotype data of 103 major rice vadeties and parental lines at the 24 marker loci was constructed and analyzed.

关键词： simple sequence repeat rice (Oryza sativa) variety identification frequency of polymorphism database

Fine Mapping of C（Chromogen for Anthocyanin） Gene in Rice

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Rice science 2008年第1期15卷 1-6页

作者： FAN Fang-jun FAN Ye-yang DU Jing-hong zhuang jie-yun Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006 China

Seven residual heterozygous lines （RHLs） displaying different genotypic compositions in the genomic region covering probable locations of C （Chromogen for anthocyanin） gene on the short arm of rice chromosome 6 were selected from the progenies of the indica cross Zhenshan 97B/Milyang 46. Seeds were harvested from each of the seven plants, and the resultant F2：3 populations were used for fine mapping of C gene. It was shown in the populations that the apiculus coloration matched to basal leaf sheath coloration in each plant. By relating the coloration performances of the populations with the genotypic compositions of the RHLs, the C locus was located between rice SSR markers RM314 and RM253. By using a total of 1279 F2：3 individuals from two populations showing coloration segregation, the C locus was then located between RM111 and RM253, with genetic distances of 0.7 cM to RM111 and 0.4 cM to RM253. Twenty-two recombinants found in the two populations were assayed with seven more markers located between RM111 and RM253, including six SSR markers and one marker for the C gene candidate, OsCl. The C locus was delimited to a 59.3-kb region in which OsC1 was located.

关键词： C locus fine mapping candidate gene residual heterozygous line rice