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An Asymptotic UTD Solution for the Scattering by an Impedance Wedge at Skew Incidence

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Wuhan University Journal of Natural Sciences 2005年第5期10卷 855-861页

作者： YUAN Fei XIAO Dong-shan zhu guo-qiang School of Electronic Information Wuhan UniversityWuhan 430072 Hubei China Kenbotong Communication LTD Foshan 528000 Guangdong China

Asymptotic expressions for the fields scattered by an isotropic impedance wedge at skew incidence are derived in the frame of the uniform geometrical theory of diffraction （UTD）. They are obtained by approximately solving the difference equations generated from impedance boundary con ditions （IBCs） in Maliuzhinets＇ approach. The method overcomes the discontinuity of the nonuniform evaluation given in the literature referred and can give out satisfactory numerical results.

关键词： electromagnetic scattering impedance wedge diffraction UTD

Comprehensive Analysis of Expressed Sequence Tags from the Pulp of the Red Mutant ‘Cara Cara’ Navel Orange (Citrus sinensis Osbeck)

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Journal of Integrative Plant Biology 2010年第10期52卷 856-867页

作者： Jun-Li Ye An-Dan zhu Neng-guo Tao qiang Xu Juan Xu Xiu-Xin Deng National Key Laboratory of Crop Genetic Improvement National Center of Citrus Breeding Huazhong Agricultural University Wuhan430070 China

Expressed sequence tag （EST） analysis of the pulp of the red-fleshed mutant ＇Cara Cara＇ navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. interpretation of the EST datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes, such as the type III metallothionein-like gene （6.0%）, heat shock protein （2.8%）, Cu/Zn superoxide dismutase （0.8%）, late embryogenesis abundant protein 5 （0.8%）, etc. 133 transcripts were detected to be differentially expressed between the red mutant and its orange-color wild genotype ＇Washington＇ via digital expression analysis. Among them, genes involved in metabolism, defense/stress and signal transduction were statistical overrepresented. Fifteen transcription factors, composed of NAM, ATAF, and CUC transcription factor （NAC）; myeloblastosis （MYB）; myelocytomatosis （MYC）; basic helix-loop-helix （bHLH）; basic leucine zipper （bZIP） domain members, were also included. The data reflected the distinct expression profile and the unique regulatory module associated with these two genotypes. Eight differently expressed genes analyzed in digital were validated by quantitative real- time polymerase chain reaction. For structural polymorphism, both simple sequence repeats and single nucleotide polymorphisms （SNP） loci were surveyed; dinucleotide presentation revealed a bias toward AG/GA/TC/CT repeats （52.5%）, against GC/CG repeats （0%）. SNPs analysis found that transitions （73%） outnumbered transversions （27%）. Seventeen potential cultivar-specific and 387 heterozygous SNP loci were detected from ＇Cara Cara＇ and ＇Washington＇ EST pool.

关键词： PLANT genetics ORANGE (Fruit) -- Varieties GENETIC polymorphisms NUCLEOTIDE sequence TRANSCRIPTION factors HEAT shock proteins EFFECT of stress on plants PLANT gene expression POLYMERASE chain reaction

Weighted gene co-expression network analysis identiffes potential regulators in response to Salmonella Enteritidis challenge in the reproductive tract of laying ducks

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Journal of Integrative Agriculture 2022年第8期21卷 2384-2398页

作者： ZHANG Yu LUO Shu-wen HOU Li-e GU Tian-tian zhu guo-qiang Wanwipa VONGSANGNAK XU Qi CHEN guo-hong Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China Jiangsu Key Laboratory of Zoonosis Yangzhou UniversityYangzhou 225009P.R.China Department of Zoology Faculty of ScienceKasetsart UniversityBangkok 10900Thailand

Salmonella Enteritidis(SE)is a zoonotic and vertically transmitted pathogen,often colonized in the reproductive tract of adult poultry,which can result in direct contamination of eggs and threaten human *** studies have revealed that some pattern recognition receptors and resistance genes were involved in regulating immune responses to SE invasion in ***,the role of these immune response genes was not independent,and the interactions among the genes remained to be further *** this study,SE burden and colonization were determined in reproductive tissue after the ducks were SE-infected,and RNA-sequencing was performed to construct co-expression networks by weighted gene co-expression network analysis(WGCNA).The result showed that SE could be isolated from 22% of infected-birds in any segment of the reproductive tract and the SE was readily colonized in the stroma,small follicle,isthmus,and vagina of the reproductive tracts in morbid *** top central,highly connected genes were subsequently identified three specific modules in the above four tissues at the defined cut-offs(P<0.01),including 60 new candidate regulators and 125 transcription ***,those 185 differentially expressed genes(DEGs)in these modules were ***,the hub genes(TRAF3,CXCR4 and IL13RA1)were identified to act with many other genes through immune response pathways including NF-kappaB,Toll-like receptor,steroid biosynthesis,and p53signaling *** data provide references that will understand the immune regulatory relationships during SE infection,but also assist in the breeding of SE-resistant lines through potential biomarkers.

关键词： duck Salmonella Enteritidis reproductive tract infection-related genes WGCNA

The tissue expression level of BPI gene in piglets from newborn to weaning and its relationship with Gram-negative bacterial infection

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Journal of Integrative Agriculture 2020年第12期19卷 3065-3073页

作者： DAI Chao-hui CAO Yue GAO Zhong-cheng zhu guo-qiang WU Sheng-long BAO Wen-bin Key Laboratory for Animal Genetics BreedingReproduction and Molecular Design of Jiangsu ProvinceCollege of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education/Yangzhou UniversityYangzhou 225009P.R.China College of Veterinary Medicine Yangzhou UniversityYangzhou 225009P.R.China

The bactericidal/permeability increasing protein (BPI) has an important function of nonspecific killing of Gram-negative bacteria. In this study, qPCR was used to detect the expression of the BPI gene in twelve tissues of Meishan piglets from birth to weaning. BPI gene overexpression, bacterial adhesions count and indirect immunofluorescence were applied to analyze the relationship between BPI gene expression and the infectivity of Escherichia coli and Salmonella. The results showed that the BPI gene was expressed highly in duodenum, jejunum and ileum (fold changes of relative expression levels were more than 10 000, 500 and 200, respectively). The expression of the BPI gene at 35 days of age was significantly higher (P<0.01) than that at all other days. Transcription of the BPI gene was up-regulated 2 401-fold in porcine intestinal epithelial (IPEC-J2) cells that were transfected with the BPI gene overexpression lentivirus (IPEC-J2-BPI), and significantly higher (P<0.01) than that in negative control cells (IPEC-J2-NC). Protein expression levels in IPEC-J2-BPI cells were also increased. When IPEC-J2 cells were incubated with E. coli and Salmonella, respectively, for 2, 4, 6, 8, 10 and 12 h, the number of bacterial adhesions in IPEC-J2-BPI cells was significantly less (P<0.05) than that in IPEC-J2-NC cells. The results of indirect immunofluorescence analysis showed that the number of bacterial adhesions in IPEC-J2-BPI cells was significantly less (P<0.01) than that in IPEC-J2-NC cells. These results demonstrated that the BPI gene might play an important role in regulating weaning stress especially intestinal-mediated immune response. Overexpression of the BPI gene at the cellular level could significantly enhance the anti-bactericidal ability against Gram-negative bacteria such as E. coli and Salmonella. This has important biological significance in piglet resistance to bacterial diarrhea.

关键词： pig BPI gene Escherichia coli Salmonella disease-resistant breeding

Load transfer laws of tensile anchors in rock masses with weak interlayers based on a shear-slipping updating model

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Journal of Mountain Science 2022年第3期19卷 812-825页

作者： WANG Yan XIANG Xin LI Chang-dong zhu guo-qiang SONG Cheng-bin XIAN Jin-ye ZHANG Jia-jun Faculty of Engineering China University of GeosciencesWuhan 430074China China Three Gorges Corporation Limited Beijing 100038China Three Gorges Research Center for Geo-hazards Ministry of Education China University of GeosciencesWuhan 430074China

The load transfer characteristics of a tensile anchor in the rock mass with weak interlayers were investigated,considering the nonuniform stress of the horizontally layered rock mass along *** improved shear-slipping model was proposed to describe the stress evolution characteristics of the bolt-rock *** on the improved model,analytical solutions of the axial force,shear stress distribution and load-displacement relationship considering the residual stress stage were *** effects of the stratigraphic sequence,pulling force and bolt diameter on the stress distribution of the anchorage interface were evaluated by using analytical *** results were verified by applying the finite difference numerical simulation *** sensitivity of each parameter to the axial force and shear stress of the rock bolt was determined based on calculation of the sensitivity *** study results show that the axial force and shear stress tend to decrease nonuniformly along the rock bolt towards the anchorage *** to the existence of weak interlayers,the shear stress mutates at the weak and hard rock interface,and the axial force appears to“rebound”at the bottom of the anchored *** has more significant effects on the axial force and shear stress at the bottom of the anchor than at the top of the *** pulling force is more sensitive to the anchor stress than stratigraphic sequence when the bolt diameter is *** study provides a theoretical framework for the fundamental problem of tensile bolts in horizontally or vertically laminated rock masses,providing a theoretical basis for anchor design.

关键词： Weak interlayer Tensile anchor Residual stress Shear-slipping updating model Sensitivity

Effective Electromagnetic Parameters and Absorbing Properties for Honeycomb Sandwich Structures with a Consideration of the Disturbing Term

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Chinese Physics Letters 2013年第10期30卷 183-186页

作者： HU Ji-Wei HE Si-Yuan RAO Zhen-Min zhu guo-qiang YIN Hong-Cheng School of Electronic Information Wuhan UniversityWuhan 430072 National Electromagnetic Scattering Laboratory Beijing 100854

The interaction between particles cannot be ignored when a high frequency electromagnetic wave is incident on a mixed *** fluctuation theory with correlation function is a suitable method to describe the *** with honeycomb sandwich structures with an absorber included are *** effective electromagnetic parameters and reflection coefficient of these materials are deduced and numerical results are *** with the method with a disturbing term not considered,this method shows better absorbing properties.

关键词： properties absorber method

Minimum Inductance Optimal Design for the Gradient Coil

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Wuhan University Journal of Natural Sciences 2002年第3期7卷 302-306页

作者： Chen Yin ping, zhu guo qiang School of Electronic Information, Wuhan University. Wuhan 430072, Hubei, China Wuhan University School of Electronic Information Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***)

In MRI (Magnetic Resonance Imaging), a crucial role of gradient coils is to image organism, meanwhile the inductance of the coils determines the speed of imaging. So it is of great importance to optimize designs of the gradient coils. The target field approach is an effective method to design the gradient coils. Having applied this method and performing many numerical tests, we achieved the designs of thex ?,y ?,z ? in which the inductance and resistance of the coils also meet the requirements.

关键词： Key words magnetic resonance imaging linearity target field Fourier transform

Dynamic Pathology and Antigen Location Study on Broiler Breeders with Coinfection of Marek's Disease Virus and Reticuloendotheliosis Virus

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Agricultural Sciences in China 2008年第11期7卷 1387-1393页

作者： DIAO Xiu-guo zhu guo CHENG Zi-qiang WANG Gui-hua MENG Xiang-kai GAO Ting-ting CUI Zhi-zhong College of Animal Science and Veterinary Medicine Shangdong Agricultural University Tai ＇an 271018 P.R. China

To further understand the generation and development of coinfection of Marek＇s disease virus （MDV） and reticuloendotheliosis virus （REV） in broiler breeders, and then find the method and optimal time of differential diagnosis for complex clinic multiple infection, the authors studied the pathohistological changes, apoptosis, immunohistochemistry （immunofluorescence）, and ultrastructure of tumor tissues of broiler breeders inoculated with MDV and REV. The study showed that proliferation of small lymphocytes was seen in the main organs at the age of 1 week, then immature lymphocytes, all kinds of lymphocytes, primitive reticulum cells, and Marek＇s disease cells （MDCs） were observed at 2-9 weeks. Apoptosis of lymphocytes could not be seen until the age of 10 weeks in the immune system. Immunohistochemistry detection showed that the positive signs of MDV and REV antigen were observed in the main organs at 2 weeks of age. Multi-morphology lymphocytes, MDV, and REV, mitotic figures and apoptosis of lymphocytes were observed with the help of transmission electron microscopy. MDV cooperating with REV promotes the course of disease of coinfection. Differential diagnosis can be done by immunohistochemistry in the early stage （before 2 weeks）, and histopathology in the late stage （post 4 weeks）. MDCs, primitive reticulum cells, immature lymphocytes, and two kinds of virions can serve as a basis for bistopathology differential diagnosis.

关键词： Marek's disease virus reticuloendotheliosis virus coinfection dynamic pathology antigen location

Apoptosis mechanisms of human gastric cancer cell line MKN-45 infected with human mutant p27

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World Journal of Gastroenterology 2005年第47期11卷 7536-7540页

作者： Jin-Shui zhu Long Wang guo-qiang Cheng Qin Li Zu-Ming zhu Li zhu Department of Gastroenterology Affiliated Sixth People's Hospital Shanghai Jiaotong University Shanghai 200233 China

AIM: To explore the inducing effect of human mutant p27 gene on the apoptosis of the human gastric cancer cell line MKN-45 and its associated mechanisms. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the human gastric cancer cell line MKN-45. Using flow cytometry, TUNEL assay and DNA fragment analysis, we measured the apoptotic effect of Ad-p27mt on the human gastric cancer cells. RESULTS: Ad-p27mt was successfully constructed and the infection efficiency reached 100%. After 18 h of infection, we observed an apoptotic hypodiploid peak on the flow cytometer before G1-S and apoptotic characteristic bands in the DNA electrophoresis. The apoptotic rate detected by TUNEL method was significantly higher in the Ad-p27mt group (89.4±3.12%)compared to the control group (3.12±0.13%, P ＜ 0.01).CONCLUSION: Human mutant p27 can induce apoptosis of the human gastric cancer cells in vitro.

关键词： Gastric cancer Human mutant p27 Cell line MKN-45