检索结果-南通市图书馆

A fungal CYP from Beauveria bassiana with promiscuous steroid hydroxylation capabilities

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第5期35卷 218-222页

作者： Yu Peng Yue Wang Tian-Jiao Chen Jing-Jing Chen Jin-Ling yang Ting Gong ping zhu State Key Laboratory of Bioactive Substance and Function of Natural Medicines NHC Key Laboratory of Biosynthesis of Natural ProductsCAMS Key Laboratory of Enzyme and Biocatalysis of Natural DrugsInstitute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

Hydroxylation of steroid core is critical to the synthesis of steroid *** sp^(3) C-H hydroxylation is challenging through chemical catalysis,alternatively,fungal biotransformation offers a possible solution to this ***,mining and metabolic engineering of cytochrome P450 monooxygenases(CYPs)is usually regarded as a more eco-friendly and efficient ***,we report the mining and identification of a new steroid CYP(CYP68BE1)from Beauveria bassiana by transcriptomics,heterologous expression,in vivo and in vitro functional *** catalytic promiscuity of CYP68BE1 was explored,and CYP68BE1 showed promiscuously and catalytically versatile,which is qualified for monohydroxylation on C11α,C1α,C6βand dihydroxylation on C1β,11αand C6β,11αof six steroids,leading to the production of key steroid intermediates required in the industrial synthesis of some indispensable steroid *** dynamics simulations were performed,revealing the molecular basis of different binding orientations of CYP68BE1 with different *** discovery of CYP68BE1 offers a promising biocatalyst for enriching the steroid structural and functional diversity,which also can be applied to biosynthesize valuable steroid drug intermediates.

关键词： Fungal CYPs Steroid hydroxylase Beauveria bassiana Promiscuous biocatalyst Molecular dynamics

Efficacy of ginseng-based Renshenguben oral solution for cancer-related fatigue among patients with advanced-stage hepatocellular carcinoma:A prospective multicenter cohort study

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2024年第3期23卷 249-256页

作者： Ming-Da Wang Chen Yuan Ke-Chun Wang Nan-ya Wang Ying-Jian Liang Hong zhu Xiang-Min Tong Tian yang Department of Hepatobiliary and Pancreatic Surgery General Surgery CenterFirst Hospital of Jilin UniversityChangchun 130021China Department of Hepatobiliary Surgery Eastern Hepatobiliary Surgery HospitalSecond Military Medical University(Naval Medical University)Shanghai 200438China Department of Clinical Laboratory Laboratory Medicine CenterZhejiang Provincial People’s HospitalHangzhou Medical CollegeHangzhou 310000China Department of Hepatobiliary Surgery First Affiliated Hospital of Harbin Medical UniversityHarbin 150007China Department of Medical Oncology First Affiliated Hospital of Soochow UniversitySuzhou 215006China fDepartment of Central Laboratory Affiliated Hangzhou First People’s HospitalWestlake University School of MedicineHangzhou 310006China

Background:Cancer-related fatigue(CRF)is a common and debilitating symptom experienced by patients with advanced-stage cancer,especially those undergoing antitumor *** study aimed to evaluate the efficacy and safety of Renshenguben(RSGB)oral solution,a ginseng-based traditional Chinese medicine,in alleviating CRF in patients with advanced hepatocellular carcinoma(HCC)receiving antitumor ***:In this prospective,open-label,controlled,multicenter study,patients with advanced HCC at BCLC stage C and a brief fatigue inventory(BFI)score of≥4 were *** were assigned to the RSGB group(RSGB,10 mL twice daily)or the control group(with supportive care).Primary and secondary endpoints were the change in multidimensional fatigue inventory(MFI)score,and BFI and functional assessment of cancer therapy-hepatobiliary(FACT-Hep)scores at weeks 4 and 8 after *** events(AEs)and toxicities were ***:A total of 409 participants were enrolled,with 206 assigned to the RSGB *** week 4,there was a trend towards improvement,but the differences were not statistically *** week 8,the RSGB group exhibited a significantly lower MFI score(P<0.05)compared to the control group,indicating improved fatigue ***,the RSGB group showed significantly greater decrease in BFI and FACT-Hep scores at week 8(P<0.05).Subgroup analyses among patients receiving various antitumor treatments showed similar *** linear regression analyses revealed that the RSGB group experienced a significantly substantial decrease in MFI,BFI,and FACT-Hep scores at week *** serious drug-related AEs or toxicities were ***:RSGB oral solution effectively reduced CRF in patients with advanced HCC undergoing antitumor therapy over an eight-week period,with no discernible *** findings support the potential of RSGB oral solution as an adjunctive treatment for managing CRF in this patient population.

关键词： Cancer-related fatigue Hepatocellular carcinoma Renshenguben oral solution Efficacy Safety Ginseng

Discovery of proqodine A derivatives with antitumor activity targeting NAD(P)H:quinone oxidoreductase 1 and nicotinamide phosphoribosyltransferase

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2024年第1期22卷 75-88页

作者： SONG Jiangzhou ZOU Guiqing ZHAO Zhou zhu ya XUE Jiayu AO Lanjia SUN Huiyong HAO Haiping ZHANG Bo XU Xiaowei State Key Laboratory of Natural Medicines Key Lab of Drug Metabolism and PharmacokineticsChina Pharmaceutical UniversityNanjing 210009China State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China

NAD(P)H:quinone oxidoreductase 1(NQO1)is a flavin protease highly expressed in various cancer ***1 catalyzes a futile redox cycle in substrates,leading to substantial reactive oxygen species(ROS)*** ROS generation results in extensive DNA damage and elevated poly(ADP-ribose)polymerase 1(PARP1)-mediated consumption of nicotinamide adenine dinucleotide(NAD^(+)),ultimately causing cell *** phosphoribosyltransferase(NAMPT),the rate-limiting enzyme in the NAD^(+)salvage synthesis pathway,emerges as a critical target in cancer *** concurrent inhibition of NQO1 and NAMPT triggers hyperactivation of PARP1 and intensive NAD+*** this study,we designed,synthesized,and assessed a novel series of proqodine A derivatives targeting both NQO1 and *** these,compound T8 demonstrated potent antitumor ***,T8 selectively inhibited the proliferation of MCF-7 cells and induced apoptosis through mechanisms dependent on both NQO1 and *** discovery offers a promising new molecular entity for advancing anticancer research.

关键词： NQO1 ROS NAMPT NAD^(+) T8

云芝糖肽配合化疗对卵巢和子宫体癌患者免疫功能及生活质量的影响(英文)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国临床康复 2006年第47期10卷 212-214页

作者：祝亚平严伟孙廷慰上海交通大学附属第一人民医院妇产科上海市200080

背景:生物反应调节剂己成为继化疗、手术及放疗后第4种治疗肿瘤的方法。云芝糖肽是一种生物反应调节剂物质,在肿瘤治疗中具有重要作用,己被应用于胃癌、肺癌、食道癌等的治疗。目的:观察云芝糖肽对因妇科恶性肿瘤而化疗的患者免疫功能... 详细信息

背景:生物反应调节剂己成为继化疗、手术及放疗后第4种治疗肿瘤的方法。云芝糖肽是一种生物反应调节剂物质,在肿瘤治疗中具有重要作用,己被应用于胃癌、肺癌、食道癌等的治疗。目的:观察云芝糖肽对因妇科恶性肿瘤而化疗的患者免疫功能与生活质量的影响。设计:对比观察。单位:上海交通大学附属第一人民医院妇产科。对象:选择1999-06/2002-12上海交通大学附属第一人民医院妇产科已手术确诊的妇科恶性肿瘤住院患者共40例。其中卵巢癌36例,平均年龄52岁;子宫体癌4例,平均年龄54岁。纳入标准:卵巢或子宫体癌患者,手术后没有化疗禁忌征。排除标准:有化疗禁忌征的患者。方法:将患者按期别及年龄配对分成试验组和对照组,每组20例。试验组接受化疗加云芝糖肽;对照组单用化疗。卵巢癌采用PAC方案化疗,子宫体癌用MFP方案。实验时机于第2次化疗开始前至第4次化疗前,共2个月。主要观察指标:各组治疗前后血免疫功能指标及生活质量指数的变化。结果:纳入患者40例,均进入结果分析。①治疗前后Karnofsky评分值试验组分别为(84±9.6),(90±8.6)分,(P0.05);治疗后试验组比对照组高10分,(P<0.05)。②治疗后试验组和对照组的CD4+分别为28.14±4.10及23.12±3.97,(P<0.05);CD4+/CD8+为0.96±0.17及0.75±0.21,(P<0.01);自然杀伤细胞为23.80±4.90,5.32±3.90,(P<0.01),试验组均明显高于对照组.结论:云芝糖肽能提高卵巢和子宫体癌患者的细胞免疫和生活质量。

关键词：糖肽类卵巢癌子宫癌症生活质量

Re-usable Cd_(0.9)Zn_(0.1)S-ZnO@C/PVDF piezo-photocatalytic film with exceptional hydrogen evolution capability triggered by the synergetic advantages of piezoelectricity and S-Scheme heterojunction

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2024年第9期96卷 164-176页

作者： ping Su Dong Zhang Minghui zhu Tengteng Liang Nan yang Huaihao Zhao Dafeng Zhang Junchang Liu Peiqing Cai Xipeng Pu School of Materials Science and Engineering Shandong Provincial Key Laboratory of Chemical Energy Science and Novel Cell TechnologyLiaocheng UniversityLiaocheng 252000ShandongChina School of Physics Science and Information Technology Shandong Key Laboratory of Optical Communication Sciencend TechnologyLiaocheng UniversityLiaocheng 252000ShandongChina College of Optical and Electronic Technology China Jiliang UniversityHangzhou 310018ZhejiangChina

Piezoelectric materials have advantages of fine-tuning photocatalytic performance through harvesting mechanical energy and open a new avenue in facilitating green catalytic ***,polyvinylidene fluoride(PVDF),a flexible piezoelectric material,was introduced to synthesize a novel Cd_(0.9)Zn_(0.1)S-ZnO@C/PVDF(CZS-ZO@C/PVDF)piezo-photocatalytic film by spin coating and immersion phase conversion *** from the piezoelectricity of PVDF and the internal electric field(IEF)of CZS-ZO@C Step-scheme(S-Scheme)heterojunction,CZS-ZO@C/PVDF was able to induce a hydrogen generation rate of 34.9 mmol g^(−1)h^(−1)activated by ultrasound and visible light(U-L),which is∼17.5 times of Cd_(0.9)Zn_(0.1)S/PVDF(CZS/PVDF)and∼7.4 times of the photocatalysis rate activated by visible light only(L).Piezoelectric measurements and COMSOL simulation illustrated the excellent piezoelectricity of CZS-ZO@C/PVDF film,which exhibits a piezoelectric coefficient(d33)of 9.9 pm V−1 and a piezoelectric potential of 874 mV(under 0.5 MPa).The reaction mechanism for the exceptional piezo-photocatalytic performance was finally disclosed through density functional theory(DFT)calculation and electrochemical *** study enriches the application scope of piezoelectric materials in sustainable energy catalysis and provides a new direction to develop efficient piezoelectric photocatalysts.

关键词： Piezo-photocatalysis Heterojunction Cd_(0.9)Zn_(0.1)S Zno@c PVDF

The dynamics beamline at SSRF

在线全文

学校读者我要写书评

暂无评论

NUCLEAR SCIENCE AND TECHNIQUES 2024年第9期35卷 1-12页

作者： Liu, Zhen Wang, Li-Hua Jiang, Yong Tong, ya-Jun zhu, Hua-Chun Ji, Te Chen, Min Jiang, Zheng Wei, Xiang-Jun Chinese Acad Sci Shanghai Adv Res Inst Shanghai 201204 Peoples R China ShanghaiTech Univ Shanghai 201210 Peoples R China

The dynamics beamline (D-Line), which combines synchrotron radiation infrared spectroscopy (SR-IR) and energy-dispersive X-ray absorption spectroscopy (ED-XAS), is the first beamline in the world to realize concurrent ED-XAS and SR-IR measurements at the same sample position on a millisecond time-resolved scale. This combined technique is effective for investigating rapid structural changes in atoms, electrons, and molecules in complicated disorder systems, such as those used in physics, chemistry, materials science, and extreme conditions. Moreover, ED-XAS and SR-IR can be used independently in the two branches of the D-Line. The ED-XAS branch is the first ED-XAS beamline in China, which uses a tapered undulator light source and can achieve approximately 2.5 x 1012 photons/s300 eV BW@7.2 keV at the sample position. An exchangeable polychromator operating in the Bragg-reflection or Laue-transmission configuration is used in different energy ranges to satisfy the requirements for beam size and energy resolution. The focused beam size is approximately 3.5 mu m (H) x 21.5 mu m (V), and the X-ray energy range is 5-25 keV. Using one- and two-dimensional position-sensitive detectors with frame rates of up to 400 kHz enables time resolutions of tens of microseconds to be realized. Several distinctive techniques, such as the concurrent measurement of in situ ED-XAS and infrared spectroscopy, time-resolved ED-XAS, high-pressure ED-XAS, XMCD, and pump-probe ED-XAS, can be applied to achieve different scientific goals.

关键词： ED-XAS SR-IR Time-resolved D-line SSRF SYNCHROTRON-RADIATION DISPERSIVE MODE SPECTROSCOPY PRESSURE EXAFS SPECTROMETER DICHROISM DETECTOR XAFS IR

前驱体转化法制备TiSi2/YSZ陶瓷涂层

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

人工晶体学报 2019年第7期48卷 1341-1349页

作者：孔诚朱亚平王帆齐会民华东理工大学材料科学与工程学院特种功能高分子材料及其相关技术教育部重点实验室

采用聚硅氮烷前驱体作为基体,添加惰性填料(YSZ)和活性填料(TiSi2)制备涂层浆料,在310s不锈钢表面利用前驱体转化法制备TiSi2/YSZ陶瓷涂层。通过TGA、XRD和SEM对涂层的物相组成和微观形貌进行了表征,并研究了TiSi2含量对涂层的隔热性能... 详细信息

采用聚硅氮烷前驱体作为基体,添加惰性填料(YSZ)和活性填料(TiSi2)制备涂层浆料,在310s不锈钢表面利用前驱体转化法制备TiSi2/YSZ陶瓷涂层。通过TGA、XRD和SEM对涂层的物相组成和微观形貌进行了表征,并研究了TiSi2含量对涂层的隔热性能以及耐高温性能的影响。结果表明,所制备的涂层厚度在15~35μm之间,由于前驱体树脂裂解过程中的体积收缩,未添加TiSi2的涂层表面分布有大量裂纹且涂层部分脱落。裂解过程中,TiSi2氧化生成TiO2与SiO2后可使TiSi2体积膨胀,弥补了前驱体树脂的体积收缩。因此随着TiSi2含量的增加,涂层脱落面积减少,表面裂纹密度降低,当TiSi2的体积分数达41%时,涂层内部无裂纹产生,该涂层样品在1200℃氧化25h后金属基底氧化增重降低88.8%,并表现出良好的隔热性能和抗热震性能。

关键词：前驱体聚合物聚硅氮烷活性填料惰性填料陶瓷涂层

重组Hepcidin融合蛋白的金属螯合亲和层析纯化

在线全文

学校读者我要写书评

暂无评论

北京化工大学学报（自然科学版） 2005年第6期32卷 15-19页

作者：张怀袁其朋朱亚平马润宇北京化工大学生命科学与技术学院北京100029

在大肠杆菌中表达的重组Hepcidin融合蛋白以包涵体形式存在,其N端带有6个组氨酸。以Ni2+-IDA-Sepharose Fast Flow为层析介质,在变性条件下以不同的咪唑和pH值洗脱方式对Hepcidin融合蛋白的纯化效果进行了比较,确定了该融合蛋白的金属... 详细信息

在大肠杆菌中表达的重组Hepcidin融合蛋白以包涵体形式存在,其N端带有6个组氨酸。以Ni2+-IDA-Sepharose Fast Flow为层析介质,在变性条件下以不同的咪唑和pH值洗脱方式对Hepcidin融合蛋白的纯化效果进行了比较,确定了该融合蛋白的金属螯合层析纯化条件。以60 mmol/L咪唑洗脱杂蛋白,然后将pH值降至4.0洗脱融合蛋白,纯化后的融合蛋白纯度大于95%,而且不含咪唑,有利于下一步Hepcidin的制备。金属螯合层析中融合蛋白收率不低于90%。Ni2+-IDA-Sepharose Fast Flow对该融合蛋白的吸附量为30.4 mg/mL。

关键词： Hepcidin 融合蛋白 6个组氨酸包涵体金属螯合亲和层析

A human-specific insertion promotes cell proliferation and migration by enhancing TBC1D8B expression

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第4期67卷 765-777页

作者： Hui Zhao Lin-Lin Liu Jian Sun Lian Jin Hai-Bing Xie Jian-Bo Li Hui Xu Dong-Dong Wu Xiao-Lin zhuang Min-Sheng Peng ya-Jun Guo Wei-zhu Qian Newton OOtecko Wei-Jie Sun Liang-Hu Qu Jie He Zhao-Li Chen Rong Liu Ce-Shi Chen ya-ping Zhang State Key Laboratory for Conservation and Utilization of Bio-resource School of Life SciencesSchool of Ecology and Environmental ScienceYunnan UniversityKunming 650091China School of Forensic Medicine Kunming Medical UniversityKunming 650500China Academy of Biomedical Engineering Kunming Medical UniversityKunming 650500China The Third Affiliated Hospital Kunming Medical UniversityKunming 650118China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyChinese Academy of SciencesKunming 650201China State Key Laboratory of Genetic Resources and Evolution Yunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunming 650201China The Key Laboratory of Gene Engineering of the Ministry of Education State Key Laboratory for BiocontrolSun Yat-sen UniversityGuangzhou 510275China National Engineering Research Center for Antibody Medicine and Shanghai Key Laboratory of Cell Engineering and Antibody Shanghai 201203China Department of Thoracic Surgery Cancer Institute and HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Human-specific insertions play important roles in human phenotypes and *** we reported a 446-bp insertion(Insert-446)in intron 11 of the TBC1D8B gene,located on chromosome X,and traced its origin to a portion of intron 6 of the EBF1 gene on chromosome ***,Insert-446 was present in the human Neanderthal and Denisovans genomes,and was fixed in humans after human-chimpanzee *** have demonstrated that Insert-446 acts as an enhancer through binding transcript factors that promotes a higher expression of human TBC1D8B gene as compared with orthologs in *** addition,over-expression TBC1D8B promoted cell proliferation and migration through“a dual finger”catalytic mechanism(Arg538 and Gln573)in the TBC domain in vitro and knockdown of TBC1D8B attenuated tumorigenesis in *** of Insert-446 prevented cell proliferation and migration in cancer and normal *** results reveal that the human-specific Insert-446 promotes cell proliferation and migration by upregulating the expression of TBC1D8B *** findings provide a significant insight into the effects of human-specific insertions on evolution.

关键词： human-specific insertion enhancer gene expression cell proliferation and migration