检索结果-南通市图书馆

The Asparagine-Rich Protein NRP Facilitates the Degradation of the PP6-type Phosphatase FyPP3 to Promote ABA Response in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2018年第2期11卷 257-268页

作者： tong zhu Yanying Wu Xiaotong Yang Wenli Chen Qingqiu Gong Xinqi Liu State Key Laboratory of Medicinal Chemical Biology Department of Biochemistry and Molecular Biology College of Life Sciences Nankai UniversityTianjin 300071 China Tianjin Key Laboratory of Protein Science Department of Plant Biology and Ecology College of Life Sciences Nankai University Tianjin 300071 China

The phytohormone abscisic acid （ABA） plays critical roles in abiotic stress responses and plant develop- ment. In germinating seeds, the phytochrome-associated protein phosphatase, FyPP3, negatively regulates ABA signaling by dephosphorylating the transcription factor ABI5. However, whether and how FyPP3 is regulated at the posttranscriptional level remains unclear. Here, we report that an asparagine-rich protein, NRP, interacts with FyPP3 and tethers FyPP3 to SYP41/61-positive endosomes for subsequent degradation in the vacuole. Upon ABA treatment, the expression of NRP was induced and NRP-mediated FyPP3 turnover was accelerated. Consistently, ABA-induced FyPP3 turnover was abolished in an nrp null mutant. On the other hand, FyPP3 can dephosphorylate NRP in vitro, and overexpression of FyPP3 reduced the half-life of NRP in vivo. Genetic analyses showed that NRP has a positive role in ABA-mediated seed germination and gene expression, and that NRP is epistatic to FyPP3. Taken together, our results identify a new regulatory circuit in the ABA signaling network, which links the intracellular trafficking with ABA signaling.

关键词： protein interaction vacuolar degradation ABA response germination

Design and Analysis of a Novel Hybrid Processing Robot Mechanism

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Automation and computing 2020年第3期17卷 403-416页

作者： Hai-Rong Fang tong zhu Hai-Qiang Zhang Hui Yang Bing-Shan Jiang School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China

In order to satisfy the requirements of large workspace and high dexterity for processing equipment of oversized cylindrical boxes′spherical crown surfaces in the aerospace industry,a novel serial-parallel hybrid processing robot mechanism is *** degrees of freedom of the 5PUS-(2UR)PU parallel mechanism are obtained by using the screw *** inverse kinematics of the hybrid mechanism are analyzed and the velocity Jacobian matrix is ***,the constraints of the main factors influencing workspace of the mechanism are given,and the position and posture workspace are ***,the dexterity and stiffness performance of the mechanism is analyzed based on the Jacobian *** virtual prototype is established,and the theoretical calculation and simulation analysis of the hybrid mechanism with arc curve as the processing trajectory are carried out by using Matlab and Adams *** research results show that the mechanism can satisfy the requirements of large workspace and high dexterity of oversized cylindrical boxes′spherical crown surface processing,and has feasibility and practical application value.

关键词： Hybrid mechanism kinematic analysis large workspace dexterity kinematic simulation

Characteristics and risk factor analyses of high-grade intraepithelial neoplasia in older patients with colorectal polyps

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2024年第10期16卷 4129-4137页

作者： Xin Zhang Ying Wang tong zhu Jian Ge Jun-Hua Yuan Department of Geriatric Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND According to the degree of intradermal neoplasia in the colorectal exhalation,it can be divided into two grades:Low-grade intraepithelial neoplasia(LGIN)and high-grade intraepithelial neoplasia(HGIN).Currently,it is difficult to accurately diagnose LGIN and HGIN through imaging,and clinical diagnosis depends on postoperative histopathological diagnosis.A more accurate method for evaluating HGIN preoperatively is urgently needed in the surgical treatment and nursing intervention of colorectal *** To explore the characteristics and risk factors of HGIN in older patients with colorectal *** We selected 84 older patients diagnosed with HGIN as the HGIN group(n=95 colonic polyps)and 112 older patients diagnosed with LGIN as the LGIN group(n=132 colonic polyps)from Shandong Provincial Hospital Affiliated to Shandong First Medical *** endoscopic features,demographic characteristics,and clinical manifestations of the two patient groups were compared,and a logistic regression model was used to analyze the risk factors for HGIN in these *** The HGIN group was older and had a higher number of sigmoid colon polyps,rectal polyps,pedunculated polyps,polyps≥1.0 cm in size,polyps with surface congestion,polyps with surface depression,and polyps with villous/tubular adenomas,a higher proportion of patients with diabetes and a family history of colorectal cancer,patients who experienced rectal bleeding or occult blood,patients with elevated carcinoembryonic antigen(CEA)and cancer antigen 199(CA199),and lower nutritional levels and higher frailty *** polyp location(in the sigmoid colon or rectum),polyp diameter(≥1.0 cm),pathological diagnosis of(villous/tubular adenoma),family history of colorectal cancer,rectal bleeding or occult blood,elevated serum CEA and CA199 levels,lower nutritional levels and higher frailty levels also are independent risk factors for *** The occurrence of high-grade neoplastic transformat

关键词： Elderly Colorectal polyps High-grade intraepithelial neoplasia Low-grade intraepithelial neoplasia Risk factors

Exogenous acid fibroblast growth factor inhibits ischemia-reperfusion-induced damage in intestinal epithelium via regulating P53 and P21WAF-1 expression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第44期11卷 6981-6987页

作者： Wei Chen Xiao-Bing Fu Shi-Li Ge tong-zhu Sun Zhi-Yong Sheng Key Research Laboratory of Wound Repair Burns Institute 304^th Clinical Department General Hospital of PLABeijing 100037 China Institute of Radiation Medicine Beijing 100850 China

AIM： To detect the effect of acid fibroblast growth factor （aFGF） on P53 and P21WAF-1 expression in rat intestine after ischemia-reperfusion （I-R） injury in order to explore the protective mechanisms of aFGF. METHODS： Hale rats were randomly divided into four groups, namely intestinal ischemia-reperfusion group （R）, aFGF treatment group （A）, intestinal ischemia group （I）, and sham-operated control group （C）. In group I, the animals were killed after 45 min of superior mesenteric artery （SHA） occlusion. In groups R and A, the rats sustained for 45 min of SHA occlusion and were treated with normal saline （0.15 mL） and aFGF （20 μg/kg, 0.15 mL）, then sustained at various times for up to 48 h after reperfusion. In group C, SHA was separated, but without occlusion. Apoptosis in intestinal villi was determined with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling technique （TUNEL）. Intestinal tissue samples were taken not only for RT- PCR to detect P53 and P21WAF-1 gene expression, but also for immunohistochemical analysis to detect P53 and P21WAF-1 protein expression and distribution. RESULTS： In histopathological study, ameliorated intestinal structures were observed at 2, 6, and 12 h after reperfusion in A group compared to R group. The apoptotic rates were （41.17±3.49）%, （42.83±5.23）%, and （53.33±6.92）% at 2, 6, and 12 h after reperfusion, respectively in A group, which were apparently lower than those in R group at their matched time points （50.67±6.95）%, （54.17±7.86）%, and （64.33±6.47）%, respectively, （P〈0.05））. The protein contents of P53 and P21WAF-1 were both significantly decreased in A group compared to R group （P〈0.05） at 2-12 h after reperfusion, while the mRNA levels of P53 and P21VVAF-1 in A group were obviously lower than those in R group at 6-12 h after reperfusion （P〈0.05）. CONCLUSION： P53 and P21WAF-1 protein accumulations are associated with intestinal barrier injury induced by I-R insult, while intravenous aFGF can all

关键词： Acid fibroblast growth factor Ischemia Reperfusion P53 gene P21WAF-1 gene

NO_x Release from Snow and Ice Covered Surface in Polar Regions and the Tibetan Plateau

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Climate Change Research 2011年第3期2卷 141-148页

作者： Feng Wang Weili Lin Junxia Wang tong zhu Centre for Atmosphere Watch ~ Services Meteorological Observation Centre . China Meteorological Administration Beijing 100081 China State Key Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering Peking University Beijing 100871 China China Quality Certification Center Beijing 100070 China

The exchanges of NOx between snow and air have significant impact on the atmospheric components and photochemical processes in the overlying boundary layer. Such exchanges increase the oxidizing capacity of the atmosphere and may have a crucial impact on the air signals that are retrieved from ice cores. In the recent years, sunlit snow and ice have been demonstrated to be important NOx sources in the polar atmospheric boundary layer. This paper makes a thorough review on the release of NOx from snow and ice, including field observations and experimental evidences, release mechanisms and influential parameters that affect such a release process, polar NOx concentrations and fluxes, and environmental impacts of the chemical processes of NOx in the polar atmospheric boundary layer. In the Tibetan Plateau, the released NOx observed recently in the sunlit snow/ice-cover is 1-order magnitude more than that in polar regions, but further scientific research is still needed to reveal its impact on the atmospheric oxidizing capacity.

关键词： NOx exchange between snow and air polar area atmospheric oxidizing capacity Tibetan Plateau

Development of a Method to Determine the Environmental Burden of Diseases and an Application to Identify Factors Driving Changes in the Number of PM_(2.5)-Related Deaths in China between 2000 and 2010

在线全文

学校读者我要写书评

暂无评论

Environment & Health 2024年第9期2卷 642-650页

作者： Ning Kang Pengfei Li Tao Xue tong zhu Institute of Reproductive and Child Health National Health Commission Key Laboratory of Reproductive Health/Department of Epidemiology and BiostatisticsMinistry of Education Key Laboratory of Epidemiology of Major Diseases(PKU)School of Public HealthPeking University Health Science CenterBeijing 100191China Institute of Medical Technology Peking University Health Science CenterBeijing 100191China Advanced Institute of Information Technology Peking UniversityHangzhou 311215China State Environmental Protection Key Laboratory of Atmospheric Exposure and Health Risk Management and Center for Environment and HealthPeking UniversityBeijing 100871China State Environmental Protection Key Laboratory of Atmospheric Exposure and Health Risk Management and Center for Environment and Health and State Key Joint Laboratory of Environment Simulation and Pollution ControlCollege of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China

The attributable burden is codetermined by the exposure level and nontarget ***,the conventional method of health impact assessment based on preestablished exposure−response functions includes only a few well-known characteristics and thus is insufficient to capture the comprehensive *** aimed to develop a method to fuse health impact assessment with epidemiological analysis and to identify factors driving baseline *** method was applied to identify the factors underlying the change in the number of fine particulate matter(PM_(2.5))related deaths in China between 2000 and *** the study period,the number of PM_(2.5)-related deaths across China's Mainland increased by 0.62(95%CI:0.57,0.69)million,with 0.65(95%CI:0.47,0.91)million,0.55(95%CI:0.39,0.79)million,and 0.11(95%CI:0.06,0.18)million deaths being associated with increased PM_(2.5)exposure,population aging,and growth in population size,***,economic growth,urbanization,improvement of welfare services,and improvement of hospital services resulted in 0.25(95%CI:0.15,0.40)million,0.16(95%CI:0.10,0.27)million,0.16(95%CI:0.09,0.26)million,and 0.09(95%CI:0.05,0.15)million fewer deaths,*** indicated that increased exposure was the major driver of the change in the number of PM_(2.5)-related deaths,and economic growth was the main driver of increased resilience to air pollution.

关键词： health burden decomposition analysis air pollution fine particulate matter premature deaths

Direct RNA sequencing in plants:Practical applications and future perspectives

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plant Communications 2024年第11期5卷 54-71页

作者： Xi-tong zhu Pablo Sanz-Jimenez Xiao-tong Ning Muhammad Tahir ul Qamar Ling-Ling Chen State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China National Key Laboratory of Crop Genetic Improvement College of InformaticsHuazhong Agricultural UniversityWuhan 430070China Integrative Omics and Molecular Modeling Laboratory Department of Bioinformatics and BiotechnologyGovernment College University Faisalabad(GCUF)Faisalabad 38000Pakistan These authors contributed equally to this article

The transcriptome serves as a bridge that links genomic variation to phenotypic diversity.A vast number of studies using next-generation RNA sequencing(RNA-seq)over the last 2 decades have emphasized the essential roles of the plant transcriptome in response to developmental and environmental conditions,providing numerous insights into the dynamic changes,evolutionary traces,and elaborate regulation of the plant *** substantial improvement in accuracy and throughput,direct RNA sequencing(DRS)has emerged as a new and powerful sequencing platform for precise detection of native and full-length transcripts,overcoming many limitations such as read length and PCR bias that are inherent to short-read ***,we review recent advances in dissecting the complexity and diversity of plant transcriptomes using DRS as the main technological approach,covering many aspects of RNA metabolism,including novel isoforms,poly(A)tails,and RNA modification,and we propose a comprehensive workflow for processing of plant DRS *** challenges to the application of DRS in plants,such as the need for machine learning tools tailored to plant transcriptomes,remain to be overcome,and together we outline future biological questions that can be addressed by DRS,such as allele-specific RNA *** technology provides convenient support on which the connection of distinct RNA features is tightly built,sustainably refining our understanding of the biological functions of the plant transcriptome.

关键词： direct RNA sequencing novel isoforms fusion transcripts poly(A)tail RNA modifications

Quantitative analysis of 3-dimensional root architecture based on image reconstruction and its application to research on phosphorus uptake in soybean

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2006年第19期51卷 2351-2361页

作者： zhu tong lin FANG Suqin LI Zhiyuan LIU Yutao LIAO Hong YAN Xiaolong Root Biology Center South China Agricultural University Guangzhou 510642 China Department of Computer Science South China Agricultural University Guangzhou 510642 China

Quantification of 3-dimensional (3-D) plant root architecture is one of the most important approaches to investigating plant root growth and its function in nutrient acquisition and utilization. However, no effective methods have been reported hitherto to quantify 3-D root architecture parameters, making it difficult to further study the 3-D characteris- tics of the root system and its function. In the present study, we created a rapid algorithm to reconstruct 3-D root system images based on the basic structural features of such linear objects as roots, using 2-D root images taken by digital CCD cameras at multi- viewing angles. This method is very effective in the reconstruction of plant root system images, thus enabling us to obtain the digital model of 3-D root architecture and its 3-D skeleton, based on which some major root architecture parameters can be calculated. Using this method, we were able to ac- quire 3-D parameters of soybean root architecture whose root diameter was more than 0.3 mm, includ- ing tap root length, total root length, average basal root angle, ratio of root width to root depth, percent- age distribution of root length in different layers and root distribution in different 3-D regions of the growth medium. We also quantitatively analyzed the rela- tionship between different root architecture parame-ters and such plant nutrition parameters as soybean biomass and phosphorus (P) uptake. Our study may provide a new tool in studying the growth and nutri- tional functions of plant root systems.

关键词：大豆根系图象再现磷效率定量分析

Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts

在线全文

学校读者我要写书评

暂无评论

Insect Science 2022年第2期29卷 539-549页

作者： Dan-tong zhu Qiong Rao Chi Zou Fei-Xue Ban Juan-Juan Zhao Shu-Sheng Liu Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects Institute of Insect SciencesZhejiang UniversityHangzhou310029 China School of Agriculture and Food Science Zhejiang Agriculture and Forestry UniversityLin'anHangzhou311300 China

Nutritional mutualism between insects and symbiotic bacteria is *** various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple secondary *** is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect *** effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well *** examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the *** whitefly complex,provisionally named as Asia II 3 and China *** sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B *** then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously *** analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were *** findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host *** phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B *** metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution.

关键词： symbiont B vitamins complement evolution whitefly