检索结果-南通市图书馆

A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum

引用

Science China chemistry 2010年第4期53卷 759-767页

作者： zhu jun, wang fangjun, dong xiaoli, ye mingliang & zou hanfa key laboratory of separation sciences for analytical chemistry National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics, Chinese Academy of sciences, Dalian 116023, China key laboratory of separation sciences for analytical chemistry Chinese Academy of Sciences Dalian China National Chromatographic Research and Analysis Center Chinese Academy of Sciences Dalian China Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian China

Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.

关键词： peptidomics label-free quantification hepatocellular carcinoma breast cancer human serum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes

引用

Protein & Cell 2012年第9期3卷 669-674页

作者： fangjun wang jun zhu Lianghai Hu Hongqiang Qin mingliang ye hanfa zou CAS key Lab of separation sciences for analytical chemistry National Chromatographic Research and Analysis CenterDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China

The human serum proteome is closely associated with the state of the *** peptides derived from proteolytic enzymes cleaving on serum proteins are widely studied due to their potential application in disease-specific marker ***,the reproducibility of peptidome analysis of endogenous peptides is significantly influenced by the proteolytic enzymes within body fluids,thereby limiting the clinical use of the endogenous *** comprehensively investigated the N and C terminus of endogenous peptides using *** cleavage site patterns of the N and C terminus and adjacent sites from all the identified endogenous peptides were highly conserved under different sample preparation conditions,including long-term incubation at 37℃ and pretreatment with repeated freeze-thaw ***,a distinguishable cleavage site pattern was obtained when a different disease serum was *** conserved cleavage site pattern derived from proteolytic enzymes holds potential in highly specific disease diagnosis.

关键词： human serum endogenous peptide N and C termini disease diagnosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

MONOLITHIC CAPILLARY COLUMNS FOR EFFICIENT separation OF BIOLOGICAL SAMPLES

MONOLITHIC CAPILLARY COLUMNS FOR EFFICIENT SEPARATION OF BIO...

引用

第十三届北京分析测试学术报告会暨展览会(BCEIA 2009)

作者： hanfa zou fangjun wang Renan WU mingliang ye CAS key laboratory of separation Science for analytical chemistry National Chromatographic R&A Center Dalian Institute of Chemical PhysicsChinese Academy of Sciences

Monolithic columns have been widely applied in capillary liquid chromatography(μLC)and capillary electrochromatography(CEC)due to their easy preparations,low back pressure,convective mass transfer process and high se...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Elevating mitochondrial reactive oxygen species by mitochondria-targeted inhibition of superoxide dismutase with a mesoporous silica nanocarrier for cancer therapy

引用

Nano Research 2014年第8期7卷 1103-1115页

作者： Yi Zhang Zhengyan Hu Guiju Xu Chuanzhou Gao Ren'an Wu hanfa zou key Lab of separation sciences for analytical chemistry National Chromatographic R&A Center Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China University of Chinese Academy of sciences Beijing 100049 China Institute of CancerStem Cell Dalian Medical University Dalian 116044 China

In the intrinsic pathway of apoptosis, stresses of mitochondrial reactive oxygen species （mitoROS） might be sensed as more effective signals than those in cytosol, as mitochondria are the major sources of reactive oxygen species （ROS） and pivotal components during cell apoptosis. Mitochondrial superoxide dismutase （SOD2） takes the leading role in eliminating mitoROS, and inhibition of SOD2 might induce severe disturbances overwhelming the mitochondrial oxidative equilibrium, which would elevate the intracellular oxidative stresses and drive cells to death. Herein, we report a general strategy to kill cancer cells by targeted inhibition of SOD2 using 2-methoxyestradiol （2-ME, an inhibitor for the SOD family） via a robust mitochondria-targeted mesoporous silica nanocarrier （mtMSN）, with the expected elevation of mitoROS and activation of apoptosis in HeLa cells. Fe304@MSN was employed in the mitochondria-targeted drug delivery and selective inhibition of mitochondrial enzymes, and was shown to be stable with good biocompatibility and high loading capacity. Due to the selective inhibition of SOD2 by 2-ME/mtMSN, enhanced elevation of mitoROS （132% of that with free 2-ME） was obtained, coupled with higher efficiency in initiating cell apoptosis （395% of that with free 2-ME in 4 h）. Finally, the 2-ME/mtMSN exhibited powerful efficacy in targeted killing of HeLa cells by taking advantage of both biological recognition and magnetic guiding, causing 97.0% cell death with only 2 Dg/mL 2-ME/mtMSN, hinting at its great potential in cancer therapy through manipulation of the delicate mitochondrial oxidative balance.

关键词： mitochondria reactive oxygen species apoptosis mesoporous silicananoparticles drug delivery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An Enzyme Assisted RP-RPLC Approach for In-Depth Human Liver Phosphoproteome Analysis

An Enzyme Assisted RP-RPLC Approach for In-Depth Human Liver...

引用

BCEIA2013分析仪器应用技术报告会

作者： Yangyang Bian Chunxiao Song Mingming dong Wenhai Jin Lihai Guo Yongming Xie mingliang ye hanfa zou key laboratory of separation sciences for analytical chemistry National Chromatographic R&A Center Dalian Institute of Chemical Physics Chinese Academy of Sciences

Reports of analysis of the human liver phosphoproteome analysis in the literature has been very limited. Here, an enzyme assisted RP-RPLC approach was developed, and we demonstrate that the introduction of trypsin digestion for fractionated Glu-C generated peptides led to a wider separation window at the second RPLC. The approach was further combined with two different types of mass spectrometers, TripleTOF 5600 system and LTQ Orbitrap Velos for the mapping of human liver phosphoproteome. TripleTOF 5600 system and LTQ Orbitrap Velos resulted in 13976 and 14050 phosphorylation sites separately, resulting in a total number of 22446 modification sites identified, corresponding to 6526 nonredundant phosphoproteins from human liver tissue. It is interested that only 25% of the total phosphorylation sites were both identified by the two instruments, this clearly demonstrated that the coverage of phosphorylation sites could be significantly increased with the using different type of mass spectrometers. So far this is the largest data set of phosphoproteome of human liver up to now.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Tuning the charge distribution and crystal field of iron single atoms via iron oxide integration for enhanced oxygen reduction reaction in zinc-air batteries

引用

Journal of Energy chemistry 2023年第10期85卷 154-163,I0006页

作者： Feifei Zhang Yinlong zhu Yijun Zhong Jing zou Yu Chen Lianhai Zu Zhouyou wang Jack Jon Hinsch Yun wang Lian Zhang Zongping Shao Huanting wang Department of Chemical and Biological Engineering Monash UniversityClaytonVIC 3800Australia Institute for Frontier Science Nanjing University of Aeronautics and AstronauticsNanjing 210016JiangsuChina WA School of Mines:Minerals Energy and Chemical Engineering(WASM-MECE)Curtin UniversityPerthWA 6102Australia key laboratory of Interfacial Physics and Technology Shanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China Monash Centre for Electron Microscopy Monash UniversityClaytonVIC 3800Australia Department of Chemical Engineering The University of MelbourneParkvilleVIC 3010Australia Centre for Catalysis and Clean Energy School of Environment and ScienceGriffith UniversityGold Coast CampusSouthportQLD 4222Australia

Metal-air batteries face a great challenge in developing efficient and durable low-cost oxygen reduction reaction(ORR)***-atom iron catalysts embedded into nitrogen doped carbon(Fe-N-C)have emerged as attractive materials for potential replacement of Pt in ORR,but their catalytic performance was limited by the symmetrical electronic structure distribution around the single-atom Fe ***,we report our findings in significantly enhancing the ORR performance of Fe-N-C by moderate Fe_(2)O_(3) integration via the strong electronic ***,the optimized catalyst(M-Fe_(2)O_(3)/Fe_(SA)@NC)exhibits excellent activity,durability and good tolerance to methanol,outperforming the benchmark Pt/C *** M-Fe_(2)O_(3)/Fe_(SA)@NC catalyst was used in a practical zinc-air battery assembly,peak power density of 155 mW cm^(-2)and specific capacity of 762 mA h g_(Zn)^(-1)were achieved and the battery assembly has shown superior cycling stability over a period of 200 *** importantly,theoretical studies suggest that the introduction of Fe_(2)O_(3) can evoke the crystal field alteration and electron redistribution on single Fe atoms,which can break the symmetric charge distribution of Fe-N_(4) and thereby optimize the corresponding adsorption energy of intermediates to promote the O_(2)*** study provides a new pathway to promote the catalytic performance of single-atom catalysts.

关键词： Single-atom catalysts Oxide nanoclusters Electronic interactions Oxygen reduction reaction Zn-air battery

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Large-scale phosphoprotein identification by Ti4+-IMAC coupled online RP-SCX-RPLC in plants

Large-scale phosphoprotein identification by Ti4+-IMAC coupl...

引用

第十四届全国植物基因组学大会

作者： Xu wang hanfa zou junxian He School of Life sciences and State key laboratory of Agrobiotechnology The Chinese University of Hong Kong key laboratory of separation sciences for analytical chemistry National Chromatographic R&A CenterDalian Institute of Chemical PhysicsChinese Academy of Sciences

Large-scale protein phosphorylation analysis by MS is emerging as a powerful tool in plant signal transduction ***,our current understanding of the phosphorylation regulatory network in plants is still very *** establ... 详细信息

关键词： protein phosphorylation phosphoproteomics phosphorylation motif Ti4+-IMAC Arabidopsis thaliana

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

纳米二氧化硅影响人肺癌细胞的定量糖蛋白质组学研究

引用

化学学报 2012年第19期70卷 2059-2065页

作者：胡争艳孙珍张轶吴仁安邹汉法中国科学院大连化学物理研究所中国科学院分离分析化学重点实验室国家色谱研究分析中心大连116023

纳米二氧化硅(纳米SiO2)是一种正在规模化生产的纳米材料,无定型纳米SiO2因其吸入和口服对生命体不会造成直接的危害被认为是生物安全的纳米材料,已被广泛用于疾病诊断、生物分析和成像、药物载体等的研究中,导致其进入人体的方式日益增... 详细信息

纳米二氧化硅(纳米SiO2)是一种正在规模化生产的纳米材料,无定型纳米SiO2因其吸入和口服对生命体不会造成直接的危害被认为是生物安全的纳米材料,已被广泛用于疾病诊断、生物分析和成像、药物载体等的研究中,导致其进入人体的方式日益增多,因此它对人体健康影响的研究对于其作为生物材料真正实现广泛应用尤为重要.本文采用肼化学方法为基础的定量蛋白质组学对无定型纳米SiO2进入人肺癌细胞后产生的影响进行了分析,结果表明其进入细胞后,导致细胞内的平衡状态发生变化,从而影响了细胞内许多重要的蛋白质的表达水平.部分跨膜蛋白质表达的变化对纳米SiO2进入细胞的途径的阐明有一定的指导意义.

关键词：纳米二氧化硅肼化学定量糖蛋白质组学跨膜蛋白质:人肺癌细胞

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：