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Expression of β-catenin Protein in Hepatocellular Carcinoma and Its Relationship with Alpha-fetoprotein

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Journal of huazhong University of Science and Technology(Medical Sciences) 2016年第6期36卷 846-851页

作者： Ya-jun REN Tao huaNG Hong-lu YU Li ZHANG Qian-jin HE Zhi-fan XIONG hua PENG Division of Gastroenterology 4Department of Urology Liyuan Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430077 China Division of Pediatrics Tianyou Hospital Wuhan University of Science and Technology Wuhan 430064 China Department of General Surgery Center Hospital of Huanggang Huanggang 438099 China

This study aimed to investigate the expression of β-catenin in hepatocellular carcinoma（HCC） tissues and its relationship with α-fetoprotein（AFP） in *** was used to determine the expression of β-catenin in normal liver tissues（n=10）,liver cirrhosis tissues（n=20）,and primary HCC tissues（n=60）.The relationship between β-catenin expression and clinical parameters of HCC was ***-time PCR and Western blotting were used to detect the m RNA and protein expression levels of β-catenin in the liver cancer cell line SMMC-7721 transfected with a plasmid encoding AFP,and also the m RNA and protein expression levels of β-catenin were measured in the liver cancer cell line Huh7 before and after the transfection with AFP sh RNA *** results showed that β-catenin was only expressed on the cell membrane in normal liver *** localization to the cytoplasm and nucleus of cells was observed in a small proportion of cirrhotic tissues or adjacent HCC tissues,and such ectopic expression of β-catenin was predominant in HCC *** abnormal expression of β-catenin was correlated with serum AFP levels,cancer cell differentiation and vascular invasion（P〈0.05）.Additionally,the increased expression of AFP resulted in the upregulation of β-catenin m RNA and protein levels,while knockdown of AFP with AFP sh RNA led to significantly decreased β-catenin m RNA and protein levels（P〈0.05）.It was suggested that the abnormal expression of β-catenin is implicated in hepatic carcinogenesis and *** can lead to increased expression of β-catenin,which may account for the poor prognosis of AFP-associated HCC patients.

关键词： β-catenin hepatocellular carcinoma α-fetoprotein

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论教师的教材素养及其提升

湖南科技大学学报（社会科学版） 2020年第4期23卷 180-184页

作者：朱华湖南科技大学教育学院湖南湘潭411201

教材变革呼唤教师的教材素养。教材素养作为教师专业素养的重要内容之一,在一定程度上支配着教师的专业水平、教育教学行为以及学生的全面发展。教师的教材观念是教材素养的核心,教材理解能力、教材运用能力、教材编创能力是教师教材素... 详细信息

教材变革呼唤教师的教材素养。教材素养作为教师专业素养的重要内容之一,在一定程度上支配着教师的专业水平、教育教学行为以及学生的全面发展。教师的教材观念是教材素养的核心,教材理解能力、教材运用能力、教材编创能力是教师教材素养的基本构成。修炼课程意识,明确教材定位;加强理论自觉,养成教材反思能力;培育教研文化,提高教材运用水平是提升教师教材素养的可能策略。

关键词：教材素养教材观课程意识教材反思

Single-nucleotide polymorphism screening and RNA sequencing of key messenger RNAs associated with neonatal hypoxic-ischemia brain damage

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Neural Regeneration Research 2020年第1期15卷 86-95页

作者： Liu-Lin Xiong Lu-Lu Xue Mohammed Al-Hawwas jin huang Rui-Ze Niu Ya-Xin Tan Yang Xu Ying-Ying Su Jia Liu Ting-hua Wang Department of Anesthesiology National Traditional Chinese Medicine Clinical Research Base and Western Medicine Translational Medicine Research CenterAffiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhouSichuan ProvinceChina School of Pharmacy and Medical Sciences Division of Health SciencesUniversity of South AustraliaAdelaideSouth AustraliaAustralia Department of Animal Zoology Kunming Medical UniversityKunmingYunnan ProvinceChina Institute of Neurological Disease Department of AnesthesiologyTranslational Neuroscience CenterWest China HospitalSichuan UniversityChengduSichuan ProvinceChina

A single-nucleotide polymorphism(SNP)is an alteration in one nucleotide in a certain position within a *** are associated with disease ***,the influences of SNPs on the pathogenesis of neonatal hypoxic-ischemic brain damage remain ***-day-old rats were used to establish a hypoxic ischemic encephalopathy *** and expression profiles of mRNAs were analyzed in hypoxic ischemic encephalopathy model rats using RNA *** exhibiting SNPs associated with hypoxic ischemic encephalopathy were identified and studied by gene ontology and pathway analysis to identify their possible involvement in the disease *** identified 89 up-regulated genes containing SNPs that were mainly located on chromosome 1 and *** ontology analysis indicated that the up-regulated genes containing SNPs are mainly involved in angiogenesis,wound healing and glutamatergic synapse and biological processing of calcium-activated chloride *** pathway analysis indicated that the differentially expressed genes play a role in glutamatergic synapses,long-term depression and oxytocin ***,intersection analysis of high throughput screening following PubMed retrieval and RNA sequencing for SNPs showed that CSRNP1,DUSP5 and LRRC25 were most relevant to hypoxic ischemic *** up-regulation of genes was confirmed by quantitative real-time polymerase chain reaction analysis of oxygen-glucose-deprived human fetal cortical *** results indicate that CSRNP1,DUSP5 and LRRC25,containing SNPs,may be involved in the pathogenesis of hypoxic ischemic *** findings indicate a novel direction for further hypoxic ischemic encephalopathy *** animal study was approved on February 5,2017 by the Animal Care and Use Committee of Kunming Medical University,Yunnan Province,China(approval ***2019038).Cerebral tissue collection from a human fetus was approved on September 30,2015 by the Eth

关键词： CSRNP1 DUSP5 gene ontology analysis human fetal cortical neurons LRRC25 mRNA neonatal hypoxic ischemic encephalopathy pathogenesis signaling pathway analysis

Deep brain implantable microelectrode arrays for detection and functional localization of the subthalamic nucleus in rats with Parkinson’s disease

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Bio-Design and Manufacturing 2024年第4期7卷 439-452页

作者： Luyi jing Zhaojie Xu Penghui Fan Botao Lu Fan Mo Ruilin Hu Wei Xu jin Shan Qianli Jia Yuxin zhu Yiming Duan Mixia Wang Yirong Wu Xinxia Cai State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China

The subthalamic nucleus(STN)is considered the best target for deep brain stimulation treatments of Parkinson’s disease(PD).It is difficult to localize the STN due to its small size and deep *** microelectrode arrays(MEAs)can rapidly and precisely locate the STN,which is important for precise *** this paper,16-channel MEAs modified with multiwalled carbon nanotube/poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate)(MWCNT/PEDOT:PSS)nanocomposites were designed and fabricated,and the accurate and rapid identification of the STN in PD rats was performed using detection sites distributed at different brain *** results showed that nuclei in 6-hydroxydopamine hydrobromide(6-OHDA)-lesioned brains discharged more intensely than those in unlesioned *** addition,the MEA simultaneously acquired neural signals from both the STN and the upper or lower boundary nuclei of the ***,higher values of spike firing rate,spike amplitude,local field potential(LFP)power,and beta oscillations were detected in the STN of the 6-OHDA-lesioned brain,and may therefore be biomarkers of STN *** with the STNs of unlesioned brains,the power spectral density of spikes and LFPs synchronously decreased in the delta band and increased in the beta band of 6-OHDA-lesioned *** may be a cause of sleep and motor disorders associated with ***,this work describes a new cellular-level localization and detection method and provides a tool for future studies of deep brain nuclei.

关键词： Functional localization Implantable microelectrode arrays Parkinson’s disease Subthalamic nucleus

Systematic Identification of Acer rubrum bZIP Transcription Factors and Their Potential Role in Anthocyanin Accumulation under Low Temperature with Light

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 3109-3130页

作者： Yue Zhao Shah Faheem Afzal zhu Chen Khan Arif Kamal Yuzhi Fei Xin Meng Jie Ren hua Liu School of Forestry&Landscape Architecture Anhui Agricultural UniversityHefei230036China Institute of Agricultural Engineering Anhui Academy of Agricultural SciencesHefei230031China

Acer rubrum is an important garden color-leafed *** leaves will turn red in autumn,which is of great orna-mental *** leaf color change in Acer rubrum is closely associated with anthocyanins *** anthocyanin synthesis and accumulation,various transcription factor families play signiﬁcant regulatory roles,including the basic(region)leucine zipper(bZIP).However,there is no report on the systematic identiﬁcation and functional analysis of the bZIPs in Acer *** this study,137 bZIPs distributed on 29 chromosomes of Acer rubrum were identiﬁed and renamed according to their locations on the *** to the constructed bZIP phylogenetic tree of Arabidopsis thaliana and Acer rubrum,bZIPs were divided into 13 *** pairs of bZIP genes were involved in tandem duplication,and 106 segmental duplication gene pairs were ***-acting elements in the promoter region of these bZIP genes were *** results of promoter analysis showed that many elements were closely related to light conditions,hormone responses,and abiotic stress *** them,the cis-acting elements related to light response were most abundant and *** results of anthocyanin determination showed that anthocyanin contents in the leaves of Acer rubrum increased signiﬁcantly under low temperature with *** addition,gene expression analysis showed that compared to other ArbZIPs,ArbZIP137,ArbZIP136,ArbZIP114,ArbZIP130,and ArbZIP14 showed a more pronounced increase in gene expression both under low-temperature conditions and under light *** the correlation analysis,there was a high correlation between ArbZIPs and several anthocyanin-regulated transcription factors,including ArMYBs,ArbHLH and ***,the bZIP genes in Acer rubrum were identiﬁed and analyzed,providing a foundational basis for future studies on their function and signiﬁcantly enhancing our understanding of the color mechanism of Acer rubrum.

关键词： Acer rubrum bZIP transcription factor light low temperature anthocyanin accumulation

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教师在思考中前行

全球教育展望 2010年第7期39卷 75-77,65页

作者：朱华黑龙江牡丹江师范学院文学院

本文基于中学教师职业这样一种现实存在,指出教师要承担思想启蒙的重任,自身应该是一位思想者。教师在教学中,要有自由的思想,独立的意识,个人的智慧,时刻关注教育教学中的问题,思课程之特点,思问题之设计等,积极探求解决问题的方法,做... 详细信息

本文基于中学教师职业这样一种现实存在,指出教师要承担思想启蒙的重任,自身应该是一位思想者。教师在教学中,要有自由的思想,独立的意识,个人的智慧,时刻关注教育教学中的问题,思课程之特点,思问题之设计等,积极探求解决问题的方法,做研究型教师,在思考中前行。

关键词：教师职业课程之特点问题之设计

河南漯河古城遗址出土宋代青白瓷化学组成及来源研究

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光谱学与光谱分析 2020年第8期40卷 2634-2639页

作者：崔名芳朱建华安徽工业大学马克思主义学院安徽马鞍山243000 安徽工业大学冶金减排与资源综合利用教育部重点实验室安徽马鞍山243000

该研究中,对发掘于河南漯河古城遗址宋金土层的瓷器样品进行了综合表征。青白瓷显微结构分析表现为典型的南方石英、绢云母质瓷;能量色散型X荧光光谱(EDXRF)分析表现出"高硅低铝"特性,胎体制作过程中采用了单一瓷石为原料的"一元配方工... 详细信息

该研究中,对发掘于河南漯河古城遗址宋金土层的瓷器样品进行了综合表征。青白瓷显微结构分析表现为典型的南方石英、绢云母质瓷;能量色散型X荧光光谱(EDXRF)分析表现出"高硅低铝"特性,胎体制作过程中采用了单一瓷石为原料的"一元配方工艺";结合胎釉外观特征及白度值,可判定其属于典型的南方青白瓷。鉴于瓷胎的成分具有一定的地域特征,借助主成分分析方法(PCA)将该批瓷器样品与南方同时代周边青白瓷瓷胎的主量化学元素进行比较,结果显示其与景德镇湖田窑和安徽繁昌窑样品的数据点分布具有很高的重合度,而与其他窑口样品明显偏离,表明这三个窑口的样品在化学组成上有高度的相似性。进一步将这3个窗口的胎釉常量化学元素进行散点图分析比较,发现该批样品与江西湖田窑青白瓷在化学元素组成上具有更高的共性。综合各种分析,推测该批青白瓷样品应产自宋代景德镇湖田窑,为景德镇供奉开封中上层统治者使用的瓷器,后期通过贸易流通到中原地区。结合古代文献资料,推测出其可能的流通路线。该研究对探明我国古代青白瓷的制瓷工艺、瓷器贸易流通状况及挖掘漯河古城遗址的历史文化价值具有重要意义。

关键词：漯河古城遗址青白瓷能量色散型X射线荧光光谱制瓷工艺古代贸易流通

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中国植物区系研究文献中存在的几个问题

云南植物研究 2007年第5期29卷 489-491页

作者：朱华中国科学院西双版纳热带植物园昆明分部云南昆明650223

自吴征镒发表"中国种子植物属的分布区类型"以后,以该文件为标准,中国地区性(地方性)植物区系的研究报告、论文已海量发表。一些文献中出现了概念的误用和分析方法上的错误。目前存在的主要问题是:1在做种分布区类型(地理成分)分析时,... 详细信息

自吴征镒发表"中国种子植物属的分布区类型"以后,以该文件为标准,中国地区性(地方性)植物区系的研究报告、论文已海量发表。一些文献中出现了概念的误用和分析方法上的错误。目前存在的主要问题是:1在做种分布区类型(地理成分)分析时,直接套用属分布区类型作为该属内各个种的分布区类型;2用属的分布区类型组成(谱)中各类型所占百分比的类似性(聚类)来决定各地区间植物区系的亲缘。根据植物区系学理论,属的分布区是该属内所有物种的分布区的集合,除单种属外,不能直接套用属分布区类型作为该属内各个种的分布区类型;亲缘关系疏远的不同植物属可以有同样的分布区类型,若把不同地区植物区系各地理成分的比例关系的类似性解读成各地区间植物区系的亲缘关系,显然是不适合的。地区植物区系之间的亲缘关系通常是由它们共有的植物科、属、种相似性系数,特别是共特有分类群相似系数来反映的。

关键词：植物区系研究文献问题

Correlation Between Antioxidation and the Content of Total Phenolics and Anthocyanin in Black Soybean Accessions

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Agricultural Sciences in China 2007年第2期6卷 150-158页

作者： XU jin-rui ZHANG Ming-wei LIU xing-hua LIU Zhang-xiong ZHANG Rui-fen SUN Ling QIu Li-juan Key Laboratory of Functional Food Ministry of Agriculture/Bio-Tech Research Institute Guangdong Academy of Agricultural Sciences Guangzhou 51064 P.R.China School of Public Health Guangdong Pharmaceutical University Guangzhou 510310 P.R.China College of Food Science and Engineering Northwest A＆F University YangUng 712100 P.R.China National Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm and Biotechnology Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

The objective of this study was to investigate the correlations between antioxidations and the contents of the total phenolics and anthocyanin in 127 accessions of black soybean. A T-test, a fast clustering procedure, and a correlation coefficient analysis were used for experimentation. The variation ranges of the total antioxidant capacity （TAC）, the total phenolics, and anthocyanin contents in 127 black soybean accessions were 0.44-3.56, 7.05-74.82, and 0.22-1.87 mg g-l, respectively, displaying significant genotype differences. The major differences in TAC, the total phenolics, and the anthocyanin contents existed among various types of accessions from geographical regions. The differences between the accessions from black and yellow soybeans, spring and autumn, summer and autumn, Dongbeichun and Nanfangchun, Dongbeichun and Nanfangxia, Beifangchun and Nanfangchun, and Beifangchun and Nanfangxia were significant at 0.01 or 0.05 levels, respectively. The general tendency was that the TAC, the total phenolics, and the anthocyanin contents of Beifangchun accessions were higher than that of Dongbeichun ones, while that of Nanfangchun accessions were the worst. 127 black soybean accessions could be clustered into 6 clusters, which consisted of 3, 24, 20, 31, 37, and 12 accessions, respectively. The most significant （P 〈 0.01） correlations existed respectively between the TAC and the total showed that the total substances phenolics content, and the TAC and the anthocyanin content of black soybean. The results phenolics and anthocyanin in black soybean seed coat were the important antioxidation

关键词： seed coat of black soybean antioxidation total phenolics content anthocyanin content correlation