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C FLBER IS NOT IMPORTANT IN ACUPUNCTURE ANALGESIA BUT FOR DNIC

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针刺研究 1989年第Z1期 140-141页

作者： Bao Hong zhou zhengfeng Yu yingxin Han Jisheng Department of Physiology, Beijing Medical University

A lot of work had been done to explore the mechanism of acupuncture anal-gesia (AA), but there have been some important questions to be dclarified. Forcxample, what kind of afferent fiber plays an important role in AA? In orderto solve this question, we applied capsaicin topically on sciatic nerve and usedthe techniques of extracellular recording and nerve trunk recording.

关键词： NH C FLBER IS NOT IMPORTANT IN ACUPUNCTURE ANALGESIA BUT FOR DNIC WDR

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机场水泥混凝土道面土基强度和变形标准分析

同济大学学报（自然科学版） 2008年第11期36卷 1516-1520页

作者：周正峰凌建明同济大学道路与交通工程教育部重点实验室上海200092

为了确定机场水泥混凝土道面土基的合理强度,运用美国联邦航空局（Federal Aviation Administration,FAA）基于弹性层状理论的道面设计方法（LEDFAA）,对比分析了土基反应模量等道面结构参数对混凝土板厚的影响.为了确定土基的容许残余... 详细信息

为了确定机场水泥混凝土道面土基的合理强度,运用美国联邦航空局（Federal Aviation Administration,FAA）基于弹性层状理论的道面设计方法（LEDFAA）,对比分析了土基反应模量等道面结构参数对混凝土板厚的影响.为了确定土基的容许残余变形,分析了土基均匀沉降对道面使用性能的影响,以及通过有限元数值模拟方法分析了不均匀沉降对道面结构性能的影响.结果表明,土基强度对混凝土板厚度的影响较其他设计参数更为明显,反应模量宜控制在40-60 MN.m^-3;土基的均匀沉降主要影响道面的标高和排水性能,应小于10 cm;土基的不均匀沉降使道面结构与土基出现脱空,应控制其变形断面的曲率.

关键词：机场水泥混凝土道面土基强度变形标准

An axenic strain reveals the responses of Phaeodactylum tricornutum to external organic carbon

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Journal of Oceanology and Limnology 2024年第5期42卷 1621-1633页

作者： zhengfeng ZHU Zhichao HE Jian LI Chengxu zhou Yanrong LI Lin ZHANG Xiaohui LI Spiros NAGATHOS Jichang HAN College of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315832China School of Biological and Chemical Engineering Panzhihua UniversityPanzhihua 617000China Ningbo Institute of Oceanography Ningbo 315832China School of Marine Life Ningbo UniversityNingbo 315832China Earth&Life Institute Catholic University of LouvainLouvain-La-Neuve 1348Belgium

The model diatom Phaeodactylum tricornutum is considered a promising source of various high value bioproducts,and developing cultivation processes is crucial for its *** mixotrophy and heterotrophy have been recommended as effective strategies for microalgal cultivation,previous studies on *** have yielded conflicting results in terms of cultivating this *** verify the capacity of this microalga utilizing external organic carbon,both heterotrophic and mixotrophic cultivation with varied carbon sources were performed using an axenic *** results demonstrate that glycerol was the only organic carbon that substantially stimulated the growth of *** in the presence of *** acetate(NaAc)at low concentrations could also promote growth,while at high concentrations led to severe inhibition under mixotrophic *** addition of glucose imposed no appreciable impact on either cell density or biomass concentration,confirming that *** cannot metabolize external ***,a comparative analysis between mixotrophy and autotrophy was performed to reveal the influences of glycerol on the cellular metabolism based on growth performances,biochemical compositions,and chlorophyll fluorescence *** also indicate that the addition of glycerol did not have detrimental effects on the capacity of either pigments biosynthesis or photosynthesis,but enhanced the saturated fatty acids and reduced the unsaturated fatty acids.

关键词： Phaeodactylum tricornutum autotrophy mixotrophy fucoxanthin glycerol

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电驱动碳黑/液态金属/液晶弹性体复合薄膜

功能高分子学报 2022年第4期35卷 357-364,I0003页

作者：周正峰王猛东南大学化学化工学院南京211189

利用导电填料碳黑(CB)嵌入液态金属/液晶弹性体(LM/LCE)复合薄膜中,通过二次交联法制备了类似于“三明治”结构的电刺激响应碳黑/液态金属/液晶弹性体(CB/LM/LCE)复合薄膜。通过傅里叶红外光谱、差示扫描量热、广角X射线衍射、扫描电子... 详细信息

利用导电填料碳黑(CB)嵌入液态金属/液晶弹性体(LM/LCE)复合薄膜中,通过二次交联法制备了类似于“三明治”结构的电刺激响应碳黑/液态金属/液晶弹性体(CB/LM/LCE)复合薄膜。通过傅里叶红外光谱、差示扫描量热、广角X射线衍射、扫描电子显微镜、万能拉伸试验仪和电驱动响应机制等对LM/LCE和CB/LM/LCE复合薄膜分别进行表征和分析。研究结果表明,LM/LCE和CB/LM/LCE复合薄膜具有优异的力学性能和电驱动收缩可逆形变性能,在80 V电压驱动下,可实现高效电热转换,最大收缩率达到45%;在负载50 g重物的情况下,依然可以实现可逆电致收缩形变,最大收缩率为42%。

关键词：电驱动碳黑液态金属液晶弹性体软致动器

Genome-Wide Identification of Tomato (Solanum lycopersicum L.) CKX Gene Family and Expression Analysis in the Callus Tissue under Zeatin Treatment

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Phyton-International Journal of Experimental Botany 2024年第6期93卷 1143-1158页

作者： zhengfeng Lai Dongmei Lian Shaoping Zhang Yudong Ju Bizhen Lin Yunfa Yao Songhai Wu Jianji Hong zhou Li Subtropical Agriculture Research Institute Fujian Academy of Agricultural SciencesZhangzhou363005China

The cytokinin oxidase/dehydrogenase(CKX)enzyme is essential for controlling theﬂuctuating levels of endogen-ous cytokinin(CK)and has a signiﬁcant impact on different aspects of plant growth and ***-less,there is limited knowledge about CKX genes in tomato(Solanum lycopersicum L.).Here we performed genome-wide identiﬁcation and analysis of nine SlCKX family members in tomatoes using bioinformatics *** results revealed that nine SlCKX genes were unevenly distributed onﬁve chromosomes(Chr.1,Chr.4,Chr.8,Chr.10,and Chr.12).The amino acid length,isoelectric points,and molecular weight of the nine SlCKX proteins ranged from 453 to 553,5.77 to 8.59,and 51.661 to 62.494 kD,*** localization analysis indi-cated that SlCKX2 proteins were located in both the vacuole and cytoplasmic matrix;SlCKX3 and SlCKX5 pro-teins were located in the vacuole;and SlCKX1,4,6,7,8,and 9 proteins were located in the cytoplasmic ***,we observed differences in the gene structures and phylogenetic relationships of SlCKX proteins among different ***1-9 were positioned on two out of the three branches of the CKX phylogenetic tree in the multispecies phylogenetic tree construction,revealing their strong conservation within phylogenetic *** patterns of expression of CKX genes were noticed in callus cultures exposed to varying con-centrations of exogenous ZT,suggesting their roles in speciﬁc developmental and physiological functions in the regeneration *** results may facilitate subsequent functional analysis of SlCKX genes and provide valu-able insights for establishing an efﬁcient regeneration system for tomatoes.

关键词： Tomato SlCKX gene family phylogenetic relationships trans-zeatin expression pattern

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中美机场水泥混凝土道面设计方法对比分析

同济大学学报（自然科学版） 2006年第9期34卷 1196-1200页

作者：赵鸿铎凌建明周正峰同济大学道路与交通工程教育部重点实验室上海200092

对中国民航混凝土道面设计方法与美国联邦航空局(Federal Aviation Administration,FAA)混凝土道面设计方法进行对比分析,评价相互之间的差别.对不同飞机类型和交通量(包括单一机型和混合交通)条件下,不同基层厚度和面层强度的60组混凝... 详细信息

对中国民航混凝土道面设计方法与美国联邦航空局(Federal Aviation Administration,FAA)混凝土道面设计方法进行对比分析,评价相互之间的差别.对不同飞机类型和交通量(包括单一机型和混合交通)条件下,不同基层厚度和面层强度的60组混凝土道面结构进行了厚度计算分析.结果表明,单一机型条件下FAA弹性层状体系法计算的厚度比我国民航的设计方法要厚,厚度差随交通荷载的增长而增加,随面层弯拉强度的增加而减少;在混合交通条件下,FAA弹性层状体系法计算的厚度比我国的略薄;我国民航的设计方法无法适应新一代大型飞机的荷载要求.

关键词：民用机场水泥混凝土道面设计方法大型飞机

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机场刚性道面接缝传荷能力的评价

同济大学学报（自然科学版） 2010年第6期38卷 844-849页

作者：周正峰凌建明袁捷林小平西南交通大学土木工程学院四川成都610031 同济大学道路与交通工程教育部重点实验室上海201804 交通部科学研究院北京100029

基于ABAQUS有限元软件,按照"贡献面积"刚度分配原则,通过在相邻混凝土板侧面的对应结点设置弹簧单元,建立了考虑接缝传荷能力的机场刚性道面3维有限元分析模型.以重型落锤式弯沉仪(HWD)弯沉测试为基础,针对不同地基强度上的典型道面结构... 详细信息

基于ABAQUS有限元软件,按照"贡献面积"刚度分配原则,通过在相邻混凝土板侧面的对应结点设置弹簧单元,建立了考虑接缝传荷能力的机场刚性道面3维有限元分析模型.以重型落锤式弯沉仪(HWD)弯沉测试为基础,针对不同地基强度上的典型道面结构,考虑基层对接缝传荷能力的影响,应用有限元模型,分析了HWD承载板、单轴双轮起落架、双轴双轮起落架和三轴双轮起落架等荷载形式作用在接缝一侧板边中部时道面结构的板边应力和挠度,建立了HWD承载板测试得到的挠度传荷系数与接缝刚度之间的相关关系,以及与不同荷载形式作用的应力折减系数之间的相关关系,成果可用于评价机场刚性道面的接缝传荷能力,以及道面板边应力分析.

关键词：机场刚性道面接缝传荷能力评价 3维有限元重型落锤式弯沉仪(HWD)

A Novel Approach for Identifying the Heme-Binding Proteins from Mouse Tissues

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Genomics, Proteomics & Bioinformatics 2003年第1期1卷 78-86页

作者： Xiaolei Li Xiaoshan Wang Kang Zhao zhengfeng zhou Caifeng Zhao Ren Yan Liang Lin Tingting Lei Jianning Yin Rong Wang Zhongsheng Sun Znyuan Xu Jingyue Bao Xiuqing Zhang Xiaoli Feng Siqi Liu Beijing Genomics Institute Chinese Academy of Sciences Beijing 101300China

Heme is a key cofactor in aerobic life, both in eukaryotes and prokaryotes. Because of the high reactivity of ferrous protoporphyrin IX, the reactions of heme in cells are often carried out through heme-protein complexes. Traditionally studies of heme-binding proteins have been approached on a case by case basis, thus there is a limited global view of the distribution of heme-binding proteins in different cells or tissues. The procedure described here is aimed at profiling heme-binding proteins in mouse tissues sequentially by 1) purification of heme-binding proteins by heme-agarose, an affinity chromatographic resin; 2) isolation of heme-binding proteins by SDS-PAGE or two-dimensional electrophoresis; 3) identification of heme-binding proteins by mass spectrometry. In five mouse tissues, over 600 protein spots were visualized on 2DE gel stained by Commassie blue and 154 proteins were identified by MALDI-TOF, in which most proteins belong to heme related. This methodology makes it possible to globally characterize the heme-binding proteins in a biological system.

关键词： herne heme-agarose proteomics mouse

A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein

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Genomics, Proteomics & Bioinformatics 2003年第3期1卷 207-215页

作者： Yan Ren, zhengfeng zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. Beijing Genomics Institute Chinese Academy of Sciences Beijing 101300 China.

In the face of the worldwide threat of severe acute respiratory syndrome (SARS) to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA),these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis.

关键词： SARS the spike protein expression antigenicity