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Journal of Integrative Agriculture 2020年第10期19卷 2477-2487页

作者： WANG Qian-nan HUANG Pan-pan zhou shan-yue The Key Lab of Integrated Crop Pests Management of shandong Province/College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109P.R.China

FgGCN5,a GCN5 homolog in Fusarium graminearum,plays a critical role in hyphal vegetative growth,asexual and sexual reproduction,deoxynivalenol(DON)biosynthesis and plant *** nuclear localized GCN5,four conserved sequence motifs(I-IV)are presented in the catalytic domain and a bromodomain in the *** a lysine acetyltransferase,conserved negatively charged residues are present to neutralize the protons from lysine ***,the role of conserved motifs/domains and residues in FgGCN5 are ***,we generated deletion mutant strains for each the conserved motifs/domains and a glutamate residue 130(E130)replacement *** of each conserved motif in the catalytic domain and replacement of E130 site resulted in manifold defects in hyphae growth,asexual and sexual development,DON biosynthesis,and plant *** defects in the mutant strains were similar to deletion *** deletion of the bromodomain led a significant reduction in DON production and virulence,with no effects on hyphae growth,asexual or sexual ***5 was further found to localize to the nucleus in conidia and hyphae *** conclusion,FgGCN5 encodes a nuclear localized *** conserved motifs in the catalytic domain and E130 are essential for correct functions of the *** conserved bromodomain is impotant for DON production and pathogen *** was the first report to identify the functions of conserved motifs/domains in FgGCN5,which will contribute to our understanding of the mechanism(s)by which FgGCN5 regulates ***.

关键词： FgGCN5 catalytic domain bromodomain DON virulence

A novel glycoside hydrolase 74 xyloglucanase CvGH74A is a virulence factor in Coniella vitis

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Journal of Integrative Agriculture 2020年第11期19卷 2725-2735页

作者： QIN Jia-xing LI Bao-hua zhou shan-yue The Key Lab of Integrated Crop Pests Management of shandong Province/College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109P.R.China

Grape white rot is a destructive fungal disease occurring ***,Coniella vitis was identified as the predominant pathogen causing this disease in *** the periderms of grape shoots are severely degraded by ***,it was speculated that cell wall-degrading enzymes(CWDEs)might play a key role in the pathogenesis of this ***,this study aimed to examine the hydrolytic activity of the CWDEs of *** results showed that xylanase(Xy)and xyloglucanase(XEG)had high levels of hydrolytic activity both in vitro and in ***,a high-virulence fungal strain exhibited higher levels of Xy and XEG activities compared with a low-virulence *** genome of the fungus was found to harbor two XEG-coding genes CvGH74A and CvGH74B,which belonged to the glycoside hydrolase(GH)74 *** expression level of CvGH74A was found to be high during pathogen ***74A gene deletion mutants were generated using the split-marker *** deletion of CvGH74A decreased both the hydrolytic activities of XEG and Xy and also the ability of the fungus to infect the grape *** differences in the hyphal growth,morphology of colonies,or conidiation were found between theΔCvGH74A mutant strains and the wild-type ***,these results suggested that CvGH74A acted as an important virulence factor,and its enzymatic activity might regulate the virulence of the *** study was novel in reporting that GH74 XEG acted as a virulence factor in ***.

关键词： Coniella vitis GH74 grape white rot virulence xyloglucanase

Involvement of the autophagy-related gene BdATG8 in development and pathogenicity in Botryosphaeria dothidea

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Journal of Integrative Agriculture 2022年第8期21卷 2319-2328页

作者： LIU Na LIAN Sen zhou shan-yue WANG Cai-xia REN Wei-chao LI Bao-hua College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109P.R.China

Botryosphaeria dothidea is a destructive fungal pathogen that causes Botryosphaeria canker and fruit ring rot on apple *** is a process of self-degradation that maintains intracellular homeostasis via lysosomal *** date,the biological role of autophagy in *** remains *** this study,we identified and characterized the autophagy-related gene BdATG8 in ***8 was able to restore the defect in nitrogen starvation tolerance of Saccharomyces cerevisiae ATG8 deletion ***-BdAtg8 was shown to be a useful marker for monitoring autophagy in *** deletion of Bd ATG8(ΔBd Atg8)blocked autophagy and significantly impaired mycelial growth,conidiation and perithecium *** addition,ΔBdAtg8 showed significantly increased sensitivity to phytoalexin and oxidative stress,suggesting that BdATG8 plays critical roles in overcoming phytoalexin and reactive oxygen species(ROS)-mediated plant *** assays revealed thatΔBdAtg8 almost lost ability to infect ***,our results indicate that BdATG8 plays an important role in fungal development,stress responses and pathogenesis in ***.

关键词： Botryosphaeria dothidea autophagy BdATG8 development pathogenicity

Neopestalotiopsis eucalypti,a causal agent of grapevine shoot rot in cutting nurseries in China

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Journal of Integrative Agriculture 2022年第12期21卷 3684-3691页

作者： MA Xuan-yan JIAO Wei-qi LI Heng ZHANG Wei REN Wei-chao WU Yan ZHANG Zhi-chang LI Bao-hua zhou shan-yue College of Plant Health and Medicine Qingdao Agricultural University/Engineering Research Center for Precision Pest Management for Fruits and Vegetables of QingdaoQingdao 266109P.R.China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China shandong Zhichang Agricultural Science and Technology Development Co. Ltd.Rizhao 276500P.R.China

Grapevine(Vitis vinifera L.)is an economically important fruit crop in the world,and China ranks first in the production of grapes with approximately 15%of the world’s total ***,diseases that cause the death of grapevine shoots pose a severe threat to the production of *** this study,the fungus Neopestalotiopsis eucalypti was identified as a causal pathogen of grapevine shoot rot based on the morphology of conidia and a phylogenetic *** phylogenetic analysis was performed with three isolates based on the combined sequence of internal transcribed spacer(ITS)region of ribosomal DNA,part of the translation elongation factor 1-alpha(Tef)and theβ-tubulin(Tub2)*** three isolates were all identified as *** tests of the three fungal isolates were conducted on grapevines shoots in vitro and in *** results showed that all three fungal isolates caused severe rot lesions on the inoculated grapevine shoots,and *** was re-isolated from the inoculated grapevine ***,*** was confirmed as a causal agent of the grapevine shoot *** is the first report of *** causing grapevine shoot disease in China.

关键词： Neopestalotiopsis eucalypti grapevine shoot rot multigene phylogenetic analysis pathogenicity

Systematic analysis of the lysine acetylome in Fusarium graminerum

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Systematic analysis of the lysine acetylome in Fusarium gram...

Interfacial Zn2+-solvation regulator towards reversible and stable Zn anode

中国植物病理学会2015年学术年会

作者： zhou shan-yue LIANG Wen-xing LI Bao-du College of Agronomy and plant Protection Qingdao Agricultural University Key Lab of Integrated Crop Pests Management of shandong Province

The acetylation of lysine residues in proteins is a ubiquitous and conserved posttranslational modification（PTM）,playing a critical regulatory role in both prokaryotes and *** comprehensive investigate functions performed by acetylation of proteins in plant fungal pathogens,in this study,the Lysine acetylome was analyzed in Fusarium graminerum PH-1,which is the causal pathogen of Fusarium head blight（FHB） of wheat and *** the combination of affinity enrichment and high-resolution LC-MS/MS analysis,large-scale lysine acetylome analysis was *** total,577 lysine acetylation sites in 364 proteins were *** analysis of the acetylome showed that the acetylated proteins are involved in a wide range of cellular functions including signaling,metabolic process,response to stimulus and so on,and exhibit diverse subcellular localizations such as in nuclear,mitochondria and plasma *** summary,this study builds up a good foundation for in-depth exploration of the physiological role of the lysine acetylation in Fusarium graminerum.

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Journal of Energy Chemistry 2025年第1期100卷 684-692页

作者： Miao zhou Xiongbin Luo Hang Li shan Guo Zhuang Tong Xiaotao zhou Xu Li Zhaohui Hou Shuquan Liang Guozhao Fang College of Mechanical Engineering Key Laboratory of Hunan Province for Advanced Carbon-based Functional Materials Hunan Institute of Science and Technology School of Materials Science and Engineering Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province Central South University College of Materials and Metallurgy Guizhou University National Energy Metal Resources and New Materials Key Laboratory Central South University

Aqueous zinc-ion batteries （AZIBs） are fundamentally challenged by the instability of the electrode/electrolyte interface,predominantly due to irreversible zinc （Zn） deposition and hydrogen ***,the intricate mechanisms behind the electrochemical discrepancies induced by interfacial Zn2+-solvation and deposition behavior demand comprehensive *** molecules endowed with special functional groups （such as hydroxyl,carboxyl,etc.） have the potential to significantly optimize the solvation structure of Zn2+and regulate the interfacial electric double layer （EDL）.By increasing nucleation overpotential and decreasing interfacial free energy,these functional groups facilitate a lower critical nucleation radius,thereby forming an asymptotic nucleation model to promote uniform Zn ***,this study presents a pioneering approach by introducing trace amounts of n-butanol as solvation regulators to engineer the homogenized Zn （H-Zn） anode with a uniform and dense *** interfacial reaction and structure evolution are explored by in/ex-situ experimental techniques,indicating that the H-Zn anode exhibits dendrite-free growth,no by-products,and weak hydrogen evolution,in sharp contrast to the bare ***,the H-Zn anode achieves a remarkable Zn utilization rate of approximately 20% and simultaneously sustains a prolonged cycle life exceeding 500 ***,the H-Zn//NH4V4O10（NVO） full battery showcases exceptional cycle stability,retaining 95.04%capacity retention after 400 cycles at a large current density of 5 A *** study enlightens solvation-regulated additives to develop Zn anode with superior utilization efficiency and extended operational lifespan.

关键词： Solvation

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汽膜对熔滴水力学碎化影响的数值模拟

原子能科学技术 2017年第2期51卷 268-274页

作者：周月善钟明君熊进标林萌上海交通大学核科学与工程学院上海200240

在熔融物与冷却剂相互作用(FCI)过程中,熔滴的水力学碎化对于后续是否产生蒸汽爆炸以及爆炸的强弱程度有着重要影响。传统的熔滴水力学碎化数值研究通常只考虑液液直接接触的两相系统;而堆芯熔化后,熔融物温度在2 500K以上,熔融物周围... 详细信息

在熔融物与冷却剂相互作用(FCI)过程中,熔滴的水力学碎化对于后续是否产生蒸汽爆炸以及爆炸的强弱程度有着重要影响。传统的熔滴水力学碎化数值研究通常只考虑液液直接接触的两相系统;而堆芯熔化后,熔融物温度在2 500K以上,熔融物周围会迅速产生汽膜,导致熔滴和冷却剂之间的传热和阻力特性发生改变。本文基于PLIC-VOF(piecewise linear interface construction-volume of fluid)界面跟踪方法对有汽膜存在的三相系统中的熔滴水力学碎化过程进行了数值研究,通过分析熔滴在有无汽膜和不同边界速度触发情况下碎化过程中的界面特性,发现熔滴碎化程度随Weber数的增加而加剧,汽膜对熔滴的水力学碎化存在一定的抑制作用。

关键词：熔融物与冷却剂相互作用水力学碎化汽膜 VOF方法数值模拟

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汽车排放污染物的生态环境降解技术

化工管理 2021年第15期 54-55页

作者：周越珊茂名市环境技术中心广东茂名525000

随着当今汽车保有量的不断增加,汽车污染物排放对生态环境所造成的污染也开始越来越受到社会关注。为有效降低汽车排放的污染物对生态环境带来的污染,保障生态环境的良好发展,文章对其降解技术进行分析。希望通过本次的分析,可以对生态... 详细信息

随着当今汽车保有量的不断增加,汽车污染物排放对生态环境所造成的污染也开始越来越受到社会关注。为有效降低汽车排放的污染物对生态环境带来的污染,保障生态环境的良好发展,文章对其降解技术进行分析。希望通过本次的分析,可以对生态环境中汽车排放污染物的有效降解有所帮助,合理解决汽车排放污染物对生态环境的不利影响,保障生态环境的健康可持续发展。

关键词：生态环境汽车排放污染物降解技术

Treadmill exercise in combination with acousto-optic and olfactory stimulation improves cognitive function in APP/PS1 mice through the brain-derived neurotrophic factor-and Cygb-associated signaling pathways

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Neural Regeneration Research 2025年第9期20卷 2706-2726页

作者： Biao Xiao Chaoyang Chu Zhicheng Lin Tianyuan Fang Yuyu zhou Chuxia Zhang Jianghui shan Shiyu Chen Liping Li Department of Physiology and Pharmacology Health Science CenterNingbo UniversityNingboZhejiang ProvinceChina Ningbo Key Laboratory of Behavioral Neuroscience Health Science CenterNingbo UniversityNingboZhejiang ProvinceChina Key Laboratory of Addiction Research of Zhejiang Province NingboZhejiang ProvinceChina

A reduction in adult neurogenesis is associated with behavioral abnormalities in patients with Alzheimer's ***,enhancing adult neurogenesis represents a promising therapeutic approach for mitigating disease symptoms and ***,nonpharmacological interventions aimed at inducing adult neurogenesis are currently *** individual non-pharmacological interventions,such as aerobic exercise,acousto-optic stimulation,and olfactory stimulation,have shown limited capacity to improve neurogenesis and cognitive function in patients with Alzheimer's disease,the therapeutic effect of a strategy that combines these interventions has not been fully *** this study,we observed an age-dependent decrease in adult neurogenesis and a concurrent increase in amyloid-beta accumulation in the hippocampus of amyloid precursor protein/presenilin 1 mice aged 2-8 *** deposition became evident at 4 months,while neurogenesis declined by 6 months,further deteriorating as the disease ***,following a 4-week multifactor stimulation protocol,which encompassed treadmill running(46 min/d,10 m/min,6 days per week),40 Hz acousto-optic stimulation(1 hour/day,6 days/week),and olfactory stimulation(1 hour/day,6 days/week),we found a significant increase in the number of newborn cells(5'-bromo-2'-deoxyuridine-positive cells),immature neurons(doublecortin-positive cells),newborn immature neurons(5'-bromo-2'-deoxyuridine-positive/doublecortin-positive cells),and newborn astrocytes(5'-bromo-2'-deoxyuridine-positive/glial fibrillary acidic protein-positive cells).Additionally,the amyloid-beta load in the hippocampus *** findings suggest that multifactor stimulation can enhance adult hippocampal neurogenesis and mitigate amyloid-beta neuropathology in amyloid precursor protein/presenilin 1 ***,cognitive abilities were improved,and depressive symptoms were alleviated in amyloid precursor protein/presenilin 1 mice

关键词： acousto-optic stimulation adult neurogenesis Alzheimer's disease amyloid precursor protein/presenilin 1 mice amyloid-beta deposition brain cell apoptosis cognitive impairment depression-like behavior involuntary treadmill exercise olfactory stimulation serum metabolites

P2Y1 receptor in Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 440-453页

作者： shan Luo Yifei Wang Tatsuhiro Hisatsune Department of Integrated Biosciences The University of TokyoKashiwaJapan

Alzheimer’s disease is the most frequent form of dementia characterized by the deposition of amyloid-beta plaques and neurofibrillary tangles consisting of hyperphosphorylated *** amyloid-beta plaques has been a primary direction for developing Alzheimer’s disease treatments in the last ***,existing drugs targeting amyloid-beta plaques have not fully yielded the expected results in the clinic,necessitating the exploration of alternative therapeutic *** evidence unravels that astrocyte morphology and function alter in the brain of Alzheimer’s disease patients,with dysregulated astrocytic purinergic receptors,particularly the P2Y1 receptor,all of which constitute the pathophysiology of Alzheimer’s *** receptors are not only crucial for maintaining normal astrocyte function but are also highly implicated in neuroinflammation in Alzheimer’s *** review delves into recent insights into the association between P2Y1 receptor and Alzheimer’s disease to underscore the potential neuroprotective role of P2Y1 receptor in Alzheimer’s disease by mitigating neuroinflammation,thus offering promising avenues for developing drugs for Alzheimer’s disease and potentially contributing to the development of more effective treatments.

关键词： astrocytes neuroinflammation P2Y1 receptor purinergic receptor