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MACS-W:A modified optical clearing agent for imaging 3D cell cultures

Journal of Innovative Optical Health Sciences 2024年第2期17卷 24-34页

作者： Xiang Zhong Chao Gao Hui Li Yuening He Peng Fei Zaozao Chen zhongze gu Dan Zhu Tingting Yu Britton Chance Center for Biomedical Photonics-MoE Key Laboratory for Biomedical Photonics Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China School of Optical and Electronic Infrmation Huazhong University of Science and Technology Wuhan Hubei 430074P.R.China State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University NanjingJiangsu 210096P.R.China Institute of Biomaterials and Medical Devices Southeast University Suzhou Jiangsu 215163P.R.China

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal *** modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent *** tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or *** address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and *** our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor *** summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

关键词： Tissue optical clearing 3D cell cultures imaging

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Rapid identification of electricigens via silver-plated photonic crystal filters

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Nano Research 2016年第9期9卷 2760-2771页

作者： Delong Wang Xiangwei Zhao Xing Liu Zhongde Mu zhongze gu State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Suzhou Key Laboratory of Environment and Biosafety Suzhou Research Institute of Southeast UniversitySuzhou 215123China

Short acquisition time and small volumes of incubated bacterial cultures are ideal for the routine and rapid identification or screening of electricigens in research and applications of microbial fuel cells. In this study, a functional substrate based on colloidal photonic crystals (PCs) was developed both for the filtration and identification of electricigens by surface-enhanced Raman scattering (SERS). The fabrication of the substrate was simplified by electroless plating of silver on filtration-based self-assembled PCs on a filter membrane. The silver-plated ordered PC structure provided a 107-fold enhancement of Raman intensity compared to that obtained with a bare PC substrate. The substrate allowed for a “drop and measure” method of bacterial identification within 5 min with a 5 μL sample volume only. The results showed that not only the electricigens Geobacter sp. and Shewanella sp. could be discriminated with species and strain specificity, but also Geobacter sp. and pilus-mutated Geobacter sp. strains. The developed silver-plated PC filter offers tremendous opportunities in energetic, environmental, and clinical applications. [Figure not available: see fulltext.] © 2016, Tsinghua University Press and Springer-Verlag Berlin Heidelberg.

关键词： electricigens surface-enhanced Raman scattering (SERS) photonic crystal electroless plating

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Organoids revealed:morphological analysis of the profound next generation in-vitro model with artificial intelligence

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Bio-Design and Manufacturing 2023年第3期6卷 319-339页

作者： Xuan Du Zaozao Chen Qiwei Li Sheng Yang Lincao Jiang Yi Yang Yanhui Li zhongze gu State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China State Key Laboratory for Novel Software Technology Nanjing UniversityNanjing 210008China Key Laboratory of Environmental Medicine Engineering Ministry of EducationSchool of Public HealthSoutheast UniversityNanjing 210009China

In modern terminology,“organoids”refer to cells that grow in a specific three-dimensional(3D)environment in vitro,sharing similar structures with their source organs or *** themorphology or growth characteristics of organoids through a microscope is a commonly used method of organoid ***,it is difficult,time-consuming,and inaccurate to screen and analyze organoids only manually,a problem which cannot be easily solved with traditional *** intelligence(AI)technology has proven to be effective in many biological and medical research fields,especially in the analysis of single-cell or hematoxylin/eosin stained tissue *** used to analyze organoids,AI should also provide more efficient,quantitative,accurate,and fast *** this review,we will first briefly outline the application areas of organoids and then discuss the shortcomings of traditional organoid measurement and analysis ***,we will summarize the development from machine learning to deep learning and the advantages of the latter,and then describe how to utilize a convolutional neural network to solve the challenges in organoid observation and ***,we will discuss the limitations of current AI used in organoid research,as well as opportunities and future research directions.

关键词： Artificial intelligence Organoids Morphology Growth characteristics Deep learning Convolutional neural network

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Three-Dimensional Direct Laser Writing of PEGda Hydrogel Microstructures with Low Threshold Power using a Green Laser Beam

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Light(Advanced Manufacturing) 2021年第1期2卷 23-30页

作者： Haoyi Yu Haibo Ding Qiming Zhang zhongze gu Min gu Laboratory of Artificial-Intelligence Nanophotonics School of ScienceRMIT UniversityMelbourne 3001Australia State Key Laboratory of Bioelectronics School of Biological and Medical EngineeringSoutheast UniversityNanjing 210096China Centre for Artificial-Intelligence Nanophotonics School of Optical-Electrical and Computer EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China

Three-dimensional(3D)direct laser writing(DLW)based on two-photon polymerisation(TPP)is an advanced technology for fabricating precise 3D hydrogel micro-and nanostructures for applications in biomedical ***,the use of visible lasers for the 3D DLW of hydrogels is advantageous because it enables high fabrication resolution and promotes wound *** glycol diacrylate(PEGda)has been widely used in TPP fabrication owing to its high ***,the high laser power required in the 3D DLW of PEGda microstructures using a visible laser in a high-water-content environment limits its applications to only those below the biological laser power safety *** this study,a formula for a TPP hydrogel based on 2-hydroxy-2-methylpropiophenone(HMPP)and PEGda was developed for the fabrication of 3D DLW microstructures at a low threshold power(0.1 nJ per laser pulse at a writing speed of 10μm·s^(−1))in a high-water-content environment(up to 79%)using a green laser beam(535 nm).This formula enables the fabrication of microstructures with micrometre fabrication resolution and high mechanical strength(megapascal level)and is suitable for the fabrication of waterresponsive,shape-changing *** results will promote the utilisation of low-power 3D DLW for fabricating hydrogel microstructures using visible lasers in high-water-content environments.

关键词： visible enable precise

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Advances of multiplex and high throughput biomolecular detection technologies based on encoding microparticles

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Science China Chemistry 2011年第8期54卷 1185-1201页

作者： ZHAO XiangWei, ZHAO YuanJin & gu zhongze State Key Laboratory of Bioelectronics Southeast University, Nanjing 210096, China State Key Laboratory of Bioelectronics Southeast University Nanjing 210096 China.

The rapid developments of genomics and proteomics have driven the demand for multiplex and high throughput analysis of large numbers of biomolecules in the fields of medical diagnostics, drug discovery, and environmental monitoring. Encoding the biomolecular binding events is the key technique to fulfill this demand, in which microparticles play the most important roles. This review outlines the development of multiplex and high throughput biodetections, and highlights the most recent advances in the field of encoding microparticles, together with problems that need to be resolved.

关键词： multiplex bioassays multiplex labels encoded microcarrier binding assays high throughput screening

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Utility of TPP-manufactured biophysical restrictions to probe multiscale cellular dynamics

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Bio-Design and Manufacturing 2021年第4期4卷 776-789页

作者： Panyu Fei Haibo Ding Yu Duan Xinyi Wang Wei Hu Peng Wu Mengxiao Wei Zhengchun Peng zhongze gu Wei Chen Department of Cardiology of the Second Affiliated Hospital and Department of Cell Biology Zhejiang University School of MedicineHangzhou 310058China School of Mechanical Engineering Zhejiang UniversityHangzhou 310027China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Center for Stretchable Electronics and Nano Sensors College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Department of Cardiology of the Second Affiliated Hospital and Department of Biochemistry Zhejiang University School of MedicineHangzhou 310058China

Biophysical restrictions regulate protein diffusion,nucleus deformation,and cell migration,which are all universal and important processes for cells to perform their biological ***,current technologies addressing these multiscale questions are extremely ***,through two-photon polymerization(TPP),we present the precise,low-cost,and multiscale microstructures(micro-fences)as a versatile investigating *** nanometer-scale printing resolution and multiscale scanning capacity,TPP is capable of generating micro-fences with sizes of 0.5-1000μ*** micro-fences are utilized as biophysical restrictions to determine the fluidity of supported lipid bilayers(SLB),to investigate the restricted diffusion of Src family kinase protein Lck on SLB,and also to reveal the mechanical bending of cell nucleus and T cell climbing *** together,the proposed versatile and low-cost micro-fences have great potential in probing the restricted dynamics of molecules,organelles,and cells to understand the basics of physical biology.

关键词： Micro-fence Multiscale Protein diffusion Nucleus mechanical bending Cell-climbing ability

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Recent biomedical applications of bio-sourced materials

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Bio-Design and Manufacturing 2018年第1期1卷 26-44页

作者： Abdelrahman Elbaz Zhenzhu He Bingbing Gao Junjie Chi Enben Su Dagan Zhang Songqin Liu Hua Xu Hong Liu zhongze gu State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou S uzhou 215123 China Getein Biotech Inc. No. 9 Bofu Road Luhe District Nanjing 211505 Jiangsu China Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research School of Chemistry and Chemical Engineering Southeast University Nanjing 211189 China

Natural anisotropic nanostructures occurring in several organisms have gained more and more attention because of their obvious advantages in sensitivity, stability, security, miniaturization, portability, online use, and remote monitoring. Due to the development of research on nature-inspired bionic structures and the demand for highly efficient, low-cost microfabrication techniques, an understanding of and the ability to replicate the mechanism of structural coloration have become increasingly significant. These sophisticated structures have many unique functions and are used in many applications. Many sensors have been proposed based on their novel structures and unique optical properties. Several of these bio-inspired sensors have been used for infrared radiation/thermal, pH, and vapor techniques, among others, and have been discussed in detail, with an intense focus on several biomedical applications. However, many applications have yet to be discovered. In this review, we will describe these nanostructured materials based on their sources in nature and various structures, such as layered, hierarchical, and helical structures. In addition, we discuss the functions endowed by these structures, such as superhydrophobicity, adhesion, and high strength, enabling them to be employed in a number of applications in biomedical fields, including cell cultivation, biosensors, and tissue engineering.

关键词： Nature Bio-sourced Bio-inspired Bio-Materials Biomedical

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OOCDB:A Comprehensive,Systematic,and Real-time Organs-on-a-chip Database

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Genomics, Proteomics & Bioinformatics 2023年第2期21卷 243-258页

作者： Jian Li Weicheng Liang Zaozao Chen Xingyu Li Pan gu Anna Liu Pin Chen Qiwei Li Xueyin Mei Jing Yang Jun Liu Lincao Jiang zhongze gu School of Life Science and Technology Southeast UniversityNanjing 210096China School of Biological Science&Medical Engineering Southeast UniversityNanjing 210096China School of Computer Data&Information SciencesUniversity of Wisconsin-MadisonMadisonWI 53706USA

Organs-on-a-chip is a microfluidic microphysiological system that uses microfluidic technology to analyze the structure and function of living human cells at the tissue and organ levels in ***-on-a-chip technology,as opposed to traditional two-dimensional cell culture and animal models,can more closely simulate pathologic and toxicologic interactions between different organs or tissues and reflect the collaborative response of multiple organs to *** the fact that many organs-on-a-chip-related data have been published,none of the current databases have all of the following functions:searching,downloading,as well as analyzing data and results from the literature on ***,we created an organs-on-a-chip database(OOCDB)as a platform to integrate information about organs-on-a-chip from various sources,including literature,patents,raw data from microarray and transcriptome sequencing,several open-access datasets of organs-on-a-chip and organoids,and data generated in our *** contains dozens of sub-databases and analysis tools,and each sub-database contains various data associated with organs-on-a-chip,with the goal of providing researchers with a comprehensive,systematic,and convenient search ***,it offers a variety of other functions,such as mathematical modeling,three-dimensional modeling,and citation mapping,to meet the needs of organs-on-a-chip.

关键词： Organ Mathematical model Citation map Organs-on-a-chip

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人体器官芯片构建的研究进展

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科学通报 2023年第36期68卷 4954-4967页

作者：曾易顾忠泽东南大学数字医学工程全国重点实验室生物科学与医学工程学院南京210096

人体器官芯片通过在微流体芯片上构建微缩组织或器官来模拟人体组织或器官的结构、功能及微环境.人体器官芯片研究的提出,为药物临床前试验中动物模型的低通量、高花费、跨物种差异、重现性差等问题提供了解决方案,或将成为下一代通用... 详细信息

人体器官芯片通过在微流体芯片上构建微缩组织或器官来模拟人体组织或器官的结构、功能及微环境.人体器官芯片研究的提出,为药物临床前试验中动物模型的低通量、高花费、跨物种差异、重现性差等问题提供了解决方案,或将成为下一代通用体外评价模型.本文综述了人体器官芯片的类型与特征,总结了人体器官芯片构建的普遍设计思路,并从细胞、芯片材料与加工、微环境构建与器官芯片测量几方面对器官芯片的构建进行技术拆解,最后讨论了目前器官芯片研究所面临的挑战与机遇.

关键词：器官芯片微流体芯片微环境构建生物医学传感

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真空离子溅射法制备TiO_2:Au复合纳米纤维新型光催化剂及其降解乙醛性能研究

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化学学报 2009年第17期67卷 1981-1986页

作者：潘超顾忠泽董丽大连水产学院理学院大连116023 东南大学生物电子学国家重点实验室南京210096 大连民族学院理学院大连116023

采用静电纺丝技术与真空离子溅射相结合的方法制备了TiO2∶Au复合纳米纤维,并采用SEM和X射线电子能谱仪对其进行了表征.结果表明TiO2∶Au纳米纤维的表面形态能通过Au沉积时间得到很好的控制.同时在紫外光照射下采用乙醛体系考察了TiO2... 详细信息

采用静电纺丝技术与真空离子溅射相结合的方法制备了TiO2∶Au复合纳米纤维,并采用SEM和X射线电子能谱仪对其进行了表征.结果表明TiO2∶Au纳米纤维的表面形态能通过Au沉积时间得到很好的控制.同时在紫外光照射下采用乙醛体系考察了TiO2纳米纤维和TiO2∶Au复合纳米纤维催化剂降解乙醛性能,结果证明TiO2∶Au复合纳米纤维具有更好的催化效率,紫外光照射70min后乙醛被完全降解.

关键词：静电纺丝真空离子溅射 TiO2∶Au纳米纤维光降解

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