检索结果-南通市图书馆

Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2012年第2期9卷 184-190页

作者： Chang-Zheng Li zhong-kun liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou Nanfang Hospital Southern Medical UniversityGuangzhouChina Institute of Genetic Engineering Southern Medical UniversityGuangzhouChina School of Traditional Chinese Medicine Southern Medical UniversityGuangzhouChina School of Pharmaceutical Science Southern Medical UniversityGuangzhouChina MOE/MOH Key Laboratory of Medical Molecular Virology Shanghai Medical CollegeFudan UniversityShanghaiChina Dgen Biotech Limited Company Hong KongChina

Hepatitis B immunoglobulin （HBIG） is important in the management of hepatitis B virus （HBV） infection. Aiming to develop recombinant monoclona# antibodies as an alternative to HBIG, we report the successful identification of HBV surface antigen （HBsAg）-specific antibodies from a full-length human antibody library displayed on mammalian cell surface. Using total RNA of peripheral blood mononuclear cells of a natively immunized donor as template, the antibody repertoire was amplified. Combining four-way ligation and the FIp recombinase-mediated integration （FIp-ln） system, we constructed a mammalian cell-based, fully human, full-length antibody display library in which each cell displayed only one kind of antibody molecule. By screening the cell library using fluorescence-activated cell sorting （FACS）, eight cell clones that displayed HBsAg-specific antibodies on cell surfaces were identified. DNA sequence analysis of the antibody genes revealed three unique antibodies. FACS data indicated that fluorescent strength of expression （FSE）, fluorescent strength of binding （FSB） and relative binding ability （RBA） were all different among them. These results demonstrated that by using our antibody mammalian display and screening platform, we can successfully identify antigen-specific antibodies from an immunized full-length antibody library. Therefore, this platform is very useful for the development of therapeutic antibodies.

关键词： antibody display antibody screening full-length antibody HBsAg-specific antibody mammalian display

Traditional Chinese medicine in the treatment of Helicobacter pylorirelated gastritis:The mechanisms of signalling pathway regulations

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2025年第3期31卷 11-28页

作者： Pei-Pei Zhang liang Li Hao-Yu Qu Guang-Yu Chen Meng-Zhou Xie Yan-kun Chen School of Traditional Chinese Medicine Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina Hunan Engineering Technology Research Center for Medicinal and Functional Food Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina Key Laboratory of Traditional Chinese Medicine Heart and Lung Syndrome Differentiation and Medicated Diet and Dietotherapy Hunan University of Chinese MedicineChangsha 410200Hunan ProvinceChina School of Informatics Hunan University of Chinese MedicineChangsha 410208Hunan ProvinceChina Precision Medicine Research and Development Center Zhuhai Institute of Advanced TechnologyChinese Academy of SciencesZhuhai 519000Guangdong ProvinceChina

Helicobacter pylori-associated gastritis(HPAG)is a common condition of the gastrointestinal ***,extensive and long-term antibiotic use has resulted in numerous adverse effects,including increased resistance,gastrointestinal dysfunction,and increased recurrence *** these concerns develop,traditional Chinese medicine(TCM)may have *** is based on the concept of completeness and aims to eliminate pathogens and strengthen the *** has the potential to prevent this condition while also boosting the rate of Helicobacter pylori *** review elaborates on the mechanism of TCM treatment for HPAG based on cellular signalling pathways,which reflects the flexibility of TCM in treating diseases and the advantages of multi-level,multipathway,and multi-target treatments for HPAG.

关键词： Helicobacter pylori Helicobacter pylori-associated gastritis Traditional Chinese medicine Signalling pathway Eliminate pathogens Strengthen the body

Posttraumatic stress symptoms among Chinese university students during the COVID-19 pandemic:Prevalence,correlates,and mental health help-seeking

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2025年第1期15卷 145-152页

作者： Rui-Yao Wu Lin-Feng Ge Bao-liang zhong Department of Psychiatry The Affiliated Wuhan Mental Health CenterTongji Medical College of Huazhong University of Science&TechnologyWuhan 430012Hubei ProvinceChina Department of Psychiatry Wuhan Mental Health CenterWuhan 430012Hubei ProvinceChina

BACKGROUND Revisiting the epidemiology of posttraumatic stress symptoms(PTSSs)among university students during the coronavirus disease 2019(COVID-19)pandemic as well as understanding the mental health help-seeking behavior of individuals with PTSSs has critical implications for public mental health strategies in future medical *** To investigate the prevalence and correlates of PTSSs among university students during the first wave of the COVID-19 pandemic in China and to examine mental health help-seeking behaviors among these *** A total of 2507 Chinese university students were recruited via snowball *** students completed the Seven-item Screening Scale for Post-traumatic Stress Disorder during the first wave of the COVID-19 pandemic in ***-graphic characteristics,pandemic-related characteristics,and mental health help-seeking behaviors of students with PTSSs were also *** The prevalence of PTSSs among the participants was 28.0%.Seven significant correlates of PTSSs were identified(odds ratio=1.23-3.65,P≤0.024):Female sex,being 19 years old or older,living with others or alone,a low level of family economic status,fair or poor interpersonal relationships,severe or very severe local pandemic,and having family members diagnosed with ***,only 3.28%of the students with PTSSs reported seeking help from mental health *** the 23 students who sought help from mental health specialists,13 opted for online or telephone-based psychological *** Our data suggest that there was a high risk of PTSSs among university students and a high level of unmet mental health needs during the COVID-19 *** delivery of mental health services online or via telephone is a promising approach to address these unmet needs.

关键词： Posttraumatic stress symptoms University students COVID-19 Mental health help-seeking China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

北京协和医院25年早产状况的调查

中国医学科学院学报 2016年第5期38卷 528-533页

作者：蒋芳高劲松钟逸锋胡静杨剑秋马良坤刘俊涛中国医学科学院北京协和医学院北京协和医院妇产科北京100730

目的调查1990年至2014年北京协和医院的早产率、早产孕周分布及病因变化趋势。方法回顾性分析1990年1月1日至2014年12月31日在北京协和医院妇产科分娩的单胎和双胎孕妇,统计其中的早产胎儿数,对不同孕周早产（极早早产、早期早产、轻型... 详细信息

目的调查1990年至2014年北京协和医院的早产率、早产孕周分布及病因变化趋势。方法回顾性分析1990年1月1日至2014年12月31日在北京协和医院妇产科分娩的单胎和双胎孕妇,统计其中的早产胎儿数,对不同孕周早产（极早早产、早期早产、轻型早产）和不同病因早产（自发性早产、治疗性早产）的比例及变化趋势进行统计分析。结果 25年间北京协和医院总体早产率为7.8%,呈升高趋势,单胎和双胎的早产率趋势相同。双胎早产儿占早产儿的23.8%,该比例从早期的15.1%增加至目前的28.5%。早产的孕周分布情况是：〈28周的极早早产26例（0.7%）,28~33＋6周的早期早产1199例（33.9%）,34~36＋6周的轻型早产2310例（65.3%）。单胎和双胎早产孕周分布差异无统计学意义（z=0.844,P=0.398）。以5年为时间跨度统计早产孕周的变迁,28周前早产的比例变化不大;28~33＋6周所占比例逐渐增加（从23.8%到36.1%）;34~36＋6周所占比例逐渐减少（从75.5%到63.3%）。单胎和双胎早产儿的孕周分布随时间的变化趋势与总体变化趋势相似。早产临产、早产胎膜早破和治疗性早产分别占早产的20.2%、38.9%和40.9%。自发性早产和治疗性早产的比例在双胎或单胎中差异无统计学意义（χ~2=1.071,P=0.301）。治疗性早产的比例有所增加,常见原因依次为妊娠期高血压疾病、胎儿因素（包括胎儿宫内窘迫、胎儿生长受限）、前置胎盘、妊娠合并心脏病。结论对于综合性医院,早产率有增加的趋势,与多胎妊娠和治疗性早产的数量增加有关。降低多胎妊娠率、有效防治妊娠合并症、并发症是早产干预的重点。

关键词：早产治疗性早产早产率

在线全文

学校读者我要写书评

暂无评论

含氟新农药的研究进展

浙江化工 2019年第5期50卷 15-21,24页

作者：李姣裴丹钟良坤许天明浙江省化工研究院有限公司浙江杭州310023 浙江工业大学化学工程学院浙江杭州310014

氟化学一直是新农药创制与研发中重要的一部分。本文对近三年来获得ISO标准命名的含氟新农药进行综述和合成简介。

关键词：含氟农药杀菌剂杀虫剂除草剂进展

Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu Na Li Zhengxiang Zhou Ru Ye Liyan zhong Yashuang Xu Junhui Ren Yi liang Xiaomei Shao Jianqiao Fang Junfan Fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission

The dopaminergic system and Alzheimer's disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第9期20卷 2495-2512页

作者： Yuhan Zhang Yuan liang Yixue Gu International Medical College Chongqing Medical UniversityChongqingChina College of Pharmacy Chongqing Medical UniversityChongqingChina Department of Neurology The First Affiliated Hospital of Chongqing Medical UniversityChongqing Key Laboratory of NeurologyChongqingChina

Alzheimer's disease is a common neurodegenerative disorder in older *** its prevalence,its pathogenesis remains unclea *** addition to the most widely accepted causes,which in clude excessive amyloid-beta aggregation,tau hyperphosphorylation,and deficiency of the neurotransmitter acetylcholine,numerous studies have shown that the dopaminergic system is also closely associated with the occurrence and development of this *** is a crucial catecholaminergic neurotransmitter in the human ***-associated treatments,such as drugs that target dopamine receptor D and dopamine analogs,can improve cognitive function and alleviate psychiatric symptoms as well as ameliorate other clinical *** r,therapeutics targeting the dopaminergic system are associated with various adverse reactions,such as addiction and exacerbation of cognitive *** review summarizes the role of the dopaminergic system in the pathology of Alzheimer's disease,focusing on currently available dopamine-based therapies for this disorder and the common side effects associated with dopamine-related *** aim of this review is to provide insights into the potential connections between the dopaminergic system and Alzheimer's disease,thus helping to clarify the mechanisms underlying the condition and exploring more effective therapeutic options.

关键词： adverse drug reaction Alzheimer's disease catecholamine dopamine receptor dopamine receptor heterodimers dopaminergic system neurodegenerative disease neurotransmitter signaling pathways traditional Chinese medicine treatment

Experimental investigation of evolutive mode-I and mode-II fracture behavior of fiber-reinforced cemented paste backfill:Effect of curing temperature and curing time

在线全文

学校读者我要写书评

暂无评论

Frontiers of Structural and Civil Engineering 2023年第2期17卷 256-270页

作者： kun FANG liang CUI School of Mines China University of Mining&TechnologyXuzhou 221116China Department of Civil Engineering Lakehead UniversityOntario P7B 5E1Canada

The curing temperature-dependent cement hydration causes the nonlinear evolution of fracture behavior and properties of fiber-reinforced cemented paste backfill(CPB)and thus influences the stability of mine backfill materials in deep ***,the coupled effect of curing temperature(20,35,and 45℃)and cement hydration at different curing times(3,7,and 28 d)on the mode-I and mode-II fracture behavior and properties of fiber-reinforced CPB is investigated.A comprehensive experimental testing program consisting of semicircular bend tests,direct shear tests,measurement of volumetric water content and matric suction,TG/DTG tests,and SEM observation is carried *** results show that the coupled thermochemical effect results in strongly nonlinear development of pre-and post-peak behavior of fiber-reinforced ***,the results discover a positive linear correlation between fracture toughness and shear strength parameters and also reveal the vital role played by matric suction in the formation of fracture ***,predictive functions are developed to estimate the coupled thermochemical effect on the development of KIc and ***,the findings and the developed mathematical tools have the potential to promote the successful application of fiber-reinforced CPB technology in deep underground mines.

关键词： fiber reinforcement cemented paste backfill fracture behavior underground mine cement hydration

Epigenetic regulation of the inflammatory response in stroke

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第11期20卷 3045-3062页

作者： Jingyi liang Fei Yang Zixiao Li Qian Li School of Basic Medical Sciences Capital Medical UniversityBeijingChina Department of Neurobiology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Laboratory for Clinical Medicine Beijing Key Laboratory of Neural Regeneration and RepairCapital Medical UniversityBeijingChina Department of Neurology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina China National Clinical Research Center for Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina National Center for Healthcare Quality Management in Neurological Diseases Beijing Tiantan HospitalCapital Medical UniversityBeijingChina Chinese Institute for Brain Research BeijingChina Research Unit of Artificial Intelligence in Cerebrovascular Disease Chinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center of Digital Healthcare for Neurological Diseases BeijingChina Department of Biochemistry and Molecular Biology School of Basic Medical SciencesCapital Medical UniversityBeijingChina Beijing Key Laboratory of Cancer Invasion and Metastasis Research Capital Medical UniversityBeijingChina

Stroke is classified as ischemic or hemorrhagic,and there are few effective treatments for either *** mechanisms play a critical role in secondary brain injury following a stroke,which manifests as cytokine release,blood–brain barrier disruption,neuronal cell death,and ultimately behavioral *** the inflammatory response has been shown to mitigate this cascade of events in experimental stroke ***,in clinical trials of anti-inflammatory agents,longterm immunosuppression has not demonstrated significant clinical benefits for *** may be attributable to the dichotomous roles of inflammation in both tissue injury and repair,as well as the complex pathophysiologic inflammatory processes in *** acute harmful inflammatory responses or inducing a phenotypic shift from a pro-inflammatory to an anti-inflammatory state at specific time points after a stroke are alternative and promising therapeutic *** agents that can modulate inflammation requires a detailed understanding of the inflammatory processes of ***,epigenetic reprogramming plays a crucial role in modulating post-stroke inflammation and can potentially be exploited for stroke *** this review,we summarize current findings on the epigenetic regulation of the inflammatory response in stroke,focusing on key signaling pathways including nuclear factor-kappa B,Janus kinase/signal transducer and activator of transcription,and mitogen-activated protein kinase as well as inflammasome *** also discuss promising molecular targets for stroke *** evidence to date indicates that therapeutic targeting of the epigenetic regulation of inflammation can shift the balance from inflammation-induced tissue injury to repair following stroke,leading to improved post-stroke outcomes.

关键词： DNA methylation histone modification intracerebral hemorrhage ischemic stroke neuroinflammation neuroprotection non-coding RNA RNA methylation subarachnoid hemorrhage treatment