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Valproic acid alters differential protein expression in SH-SY5Y cells

Neural Regeneration Research 2011年第27期6卷 2134-2139页

作者： zhong dong ying wang ming chang guoyi li linsen hu Department of Neurology the First Affiliated Hospital Jilin University Changchun 130021 Jilin Province China

This study sought to identify differentially expressed proteins in SH-SY5Y cells treated with valproic acid, using two-dimensional difference gel electrophoresis analysis. Three proteins were unambi-guously identified： the eukaryotic translation initiation factor 4A isoform 1 and ATP6V1B2 protein were downregulated, while the heterogeneous nuclear ribonucleoprotein K was upregulated. Moreover, all three proteins are associated with altered expression due to oxidative stress. Ma-trix-assisted laser desorption/ionization-time of flight mass spectrometry and protein immunoblotting assay confirmed the differential expression of eukaryotic translation initiation factor 4A isoform 1. The results indicate that valproic acid exerts an antioxidation effect by regulating the expression of eukaryotic translation initiation factor 4A isoform 1.

关键词： valproic acid two-dimensional difference gel electrophoresis matrix-assisted laser desorption-ionization SH-SY5Y cells proteomics neural regeneration

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Identification of differentially expressed proteins in SH-SY5Y cells treated with resveratrol

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Neural Regeneration Research 2011年第21期6卷 1612-1617页

作者： ying wang zhong dong Hongyan Fan ming chang guoyi li linsen hu Department of Neurology First Affiliated Hospital Jilin University Changchun 130021 Jilin Province China Department of Pharmacology Jilin Medical College of Basic Medicine Jilin 132011 Jilin Province China

To gain insight into the molecular mechanisms of resveratrol-mediated neuroprotection, two-dimensional difference gel electrophoresis in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to identify proteins differentially-expressed in SH-SY5Y cells treated with resveratrol. Compared with the control group, resveratrol treatment significantly affected the expression of four proteins： endoplasmic reticulum oxidoreductin 1-like protein alpha, p21-activated kinase 1, Archain 1, and T cell receptor beta chain. The former three were downregulated and the latter was upregulated. These proteins are primarily associated with endoplasmic reticulum stress, intracellular trafficking, and immune function.

关键词： resveratrol SH-SY5Y cells two-dimensional difference gel electrophoresis proteomics neural regeneration

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