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Chromosome-scale genome of the human blood fluke Schistosoma mekongi and its implications for public health

Infectious Diseases of Poverty 2023年第6期12卷 34-50页

作者： Minyu Zhou Lian Xu Dahua Xu Wen Chen Jehangir Khan Yue Hu Hui Huang Hang Wei Yiqing Zhang Phiraphol Chusongsang Kanthi Tanasarnprasert Xiang Hu Yanin Limpanont zhiyue lv Key Laboratory of Tropical Disease Control Ministry of EducationSun Yat-Sen UniversityGuangzhouChina Department of Pathogen Biology and Biosafety Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhouChina Key Laboratory of Neuroregeneration Ministry of Education and Jiangsu ProvinceCo-Innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongChina Key Laboratory of Tropical Translational Medicine of Ministry of Education College of Biomedical Information and EngineeringHainan Medical UniversityHaikouChina Key Laboratory of Vascular Biology and Translational Medicine Medical SchoolHunan University of Chinese MedicineChangshaChina Hainan General Hospital Hainan Afliated Hospital of Hainan Medical UniversityHaikouChina Department of Social and Environmental Medicine Faculty of Tropical MedicineMahidol UniversityBangkokThailand State Key Laboratory of Developmental Biology of Freshwater Fish College of Life SciencesHunan Normal UniversityChangshaChina.

Background Schistosoma mekongi is a human blood fluke causing schistosomiasis that threatens approximately 1.5 million humans in the world. Nonetheless, the limited availableS. mekongi genomic resources have hindered understanding of its biology and parasite-host interactions for disease management and pathogen control. The aim of our study was to integrate multiple technologies to construct a high-quality chromosome-level assembly of theS. mekongi genome.Methods The reference genome forS. mekongi was generated through integrating Illumina, PacBio sequencing, 10 × Genomics linked-read sequencing, and high-throughput chromosome conformation capture (Hi-C) methods. In this study, we conducted de novo assembly, alignment, and gene prediction to assemble and annotate the genome. Comparative genomics allowed us to compare genomes across different species, shedding light on conserved regions and evolutionary relationships. Additionally, our transcriptomic analysis focused on genes associated with parasite-snail interactions inS. mekongi infection. We employed gene ontology (GO) enrichment analysis for functional annotation of these genes.Results In the present study, theS. mekongi genome was both assembled into 8 pseudochromosomes with a length of 404 Mb, with contig N50 and scaffold N50 lengths of 1168 kb and 46,759 kb, respectively. We detected that 43% of the genome consists of repeat sequences and predicted 9103 protein-coding genes. We also focused on proteases, particularly leishmanolysin-like metalloproteases (M8), which are crucial in the invasion of hosts by 12 flatworm species. Through phylogenetic analysis, it was discovered that the M8 gene exhibits lineage-specific amplification among the genusSchistosoma. Lineage-specific expansion of M8 was observed in blood flukes. Additionally, the results of the RNA-seq revealed that a mass of genes related to metabolic and biosynthetic processes were upregulated, which might be beneficial for cercaria production.Conclusions This study delivers a high-quality, chromos

关键词： Schistosoma mekongi Chromosome-scale genome Protease RNA-seq

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Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine

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Cellular & Molecular Immunology 2008年第5期5卷 385-391页

作者： Hanjiang He Wenyu Wang ZhongdaoWu zhiyue lv Jun Li Lizhi Tan Department of Parasitology Zhongshan Medical College Sun Yat-sen University Guangzhou 510080 China Key Laboratory for Tropical Disease Control Chinese Ministry of Education Guangzhou 510080 China Department of Pathogenic Biology Xiangnan University Chenzhou 423000 China Institute of Pathogenic Biology Nanhua University Hengyang 421001 China These authors contributed equally to this work

In this study, the full lipL21 gene fragment encoding outer membrane protein LipL21 was cloned from L. interrogans serovar Lai and inserted into eukaryotic expression vector pcDNA3.1（＋）. The guinea pigs were immunized with pcDNA3.1（＋）-lipL21, pcDNA3.1（＋） or PBS. Six weeks after the second immunization, the splenocytes were isolated to detect their proliferative ability by lymphocyte transformation experiments. In addition, microscopic agglutination test was used for quantitative detection of specific antibodies. The rest guinea pigs were challenged intraperitoneally with L. interogans sorevar Lai. Then, protective effect was evaluated on the basis of survival and histopathological lesions in the kidneys, lungs, and liver. The lipL21 gene was successfully expressed in COS-7 cells through recombinant pcDNA3.1（＋）-lipL21. The titer of specific antibodies substantially increased, and the stimulation index of splenocytes increased significantly. Hence, the pcDNA3.1（＋）-lipL21 could protect the immunized guinea pigs from homotypic Leptospira infection. Furthermore, no obvious pathologic changes were observed in the pcDNA3.1（＋）-lipL21 immunized guinea pigs. The results showed that the protective effect with pathogenic strains of Leptospira was shared by LipL21 mediated through a plasmid vector. Consequently, these results indicated that the lipL21 DNA vaccine was a promising candidate for the prevention of leptospirosis.

关键词： Leptospira lipL21 DNA vaccine immunoprotection

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Foodborne parasitic diseases in China:A scoping review on current situation,epidemiological trends,prevention and control

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Asian Pacific Journal of Tropical Medicine 2021年第9期14卷 385-400页

作者： Langui Song Qingxing Xie zhiyue lv The Eighth Affiliated Hospital Sun Yat-sen UniversityShenzhenGuangdongChina Key Laboratory of Tropical Translational Medicine of Ministry of Education Hainan Medical UniversityHaikouChina NHC Key Laboratory of Control of Tropical Diseases the First Affiliated HospitalHainan Medical UniversityHaikouChina Key Laboratory of Tropical Disease Control Sun Yat-sen UniversityMinistry of EducationGuangzhouChina

Objective:Foodborne parasitic diseases,although with a declining overall incidence rate,are still endangering local public safety.This review aims to describe the current situation and epidemiological trends of foodborne parasitic diseases in China in order to explore possible reasons contributors to its high prevalence in some areas,and propose strategies for prevention and control accordingly.Methods:A scoping review was conducted by searching Pub Med,CNKI,Wanfang,CQVIP,Embase,and the Cochrane Library using search formula"foodborne parasitic diseases(or foodborne parasites)"AND"China".Studies on foodborne parasitic diseases in China were considered,but only articles in English or Chinese published between January 1980 and June 2020 were retrieved.Included studies were screened according to the eligibility criteria:1)diseases consistent with the WHO definition of foodborne parasitic diseases;2)the food carriers were included in the WHO food classification;3)data related to epidemiology,pathogenicity,and prevention and control;4)Foodborne parasitic diseases cases or outbreaks in China.Results:A total of 111 out of 665 records were included and summarized.The prevalence of clonorchiasis,angiostrongyliasis,echinococcosis,trichinellosis and cysticercosis was still increasing although the infection rate of soil-transmitted nematodes has substantially decreased in recent years.Diverse eating habits,close contact with animals,and urbanization were contributing factors to the increase.Conclusions:Foodborne parasitic diseases remain an important public health issue in China with the progress of economic globalization and food diversification.We should manage to raise public awareness about the prevention and control of foodborne parasitic diseases,improve health and safety inspections,as well as public health practice.

关键词： Foodborne diseases Parasitic diseases China Preventive medicine

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Effects of dietary Clostridium butyricum and rumen protected fat on meat quality,oxidative stability,and chemical composition of finishing goats

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Journal of Animal Science and Biotechnology 2024年第2期15卷 911-924页

作者： Meimei Zhang zhiyue Zhang Xinlong Zhang Changming Lu Wenzhu Yang Xiaolai Xie Hangshu Xin Xiaotan Lu Mingbo Ni Xinyue Yang Xiaoyang lv Peixin Jiao College of Animal Science and Technology Northeast Agricultural UniversityHarbin 150030People’s Republic of China Lethbridge Research and Development Centre Lethbridge T1J 4B1Canada School of Agriculture and Food Sciences The University of QueenslandGattonQLD 4343Australia International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement Yangzhou 225009China

Background Clostridium butyricum(CB)is a probiotic that can regulate intestinal microbial composition and improve meat quality.Rumen protected fat(RPF)has been shown to increase the dietary energy density and provide essential fatty acids.However,it is still unknown whether dietary supplementation with CB and RPF exerts beneficial effects on growth performance and nutritional value of goat meat.This study aimed to investigate the effects of dietary CB and RPF supplementation on growth performance,meat quality,oxidative stability,and meat nutritional value of finishing goats.Thirty-two goats(initial body weight,20.5±0.82 kg)were used in a completely randomized block design with a 2 RPF supplementation(0 vs.30 g/d)×2 CB supplementation(0 vs.1.0 g/d)factorial treatment arrangement.The experiment included a 14-d adaptation and 70-d data and sample collection period.The goats were fed a diet consisted of 400 g/kg peanut seedling and 600 g/kg corn-based concentrate(dry matter basis).Result Interaction between CB and RPF was rarely observed on the variables measured,except that shear force was reduced(P<0.05)by adding CB or RPF alone or their combination;the increased intramuscular fat(IMF)content with adding RPF was more pronounced(P<0.05)with CB than without CB addition.The pH24h(P=0.009),a*values(P=0.007),total antioxidant capacity(P=0.050),glutathione peroxidase activities(P=0.006),concentrations of 18:3(P<0.001),20:5(P=0.003)and total polyunsaturated fatty acids(P=0.048)were increased,whereas the L*values(P<0.001),shear force(P=0.050)and malondialdehyde content(P=0.044)were decreased by adding CB.Furthermore,CB supplementation increased essential amino acid(P=0.027),flavor amino acid(P=0.010)and total amino acid contents(P=0.024)as well as upregulated the expression of lipoprotein lipase(P=0.034)and peroxisome proliferator-activated receptorγ(PPARγ)(P=0.012),and downregulated the expression of stearoyl-CoA desaturase(SCD)(P=0.034).The RPF supplementation increased dry matter intake(P=0.005),averaged daily gain(tren

关键词： Chemical composition Clostridium butyricum Goats Meat quality Oxidative stability Rumen protected fat

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Recombinant Sj16 from Schistosoma japonicum contains a functional N-terminal nuclear localization signal necessary for nuclear translocation in dendritic cells and IL-10 production

Recombinant Sj16 from Schistosoma japonicum contains a funct...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工...

作者： Xi Sun Fan Yang Jia Shen Zhen Liu Jinyi Liang Huanqin Zheng Mingqiu Fung zhiyue lv Zhongdao Wu Department of Parasitology Zhongshan School of Medicine Sun Yat-sen University Key Laboratory for Tropical Diseases Control the Ministry of Education Department of Biotherapy The 3rd Affiliated Hospital of Sun Yat-sen University Department of Biology Chinese University of Hongkong

Background: Sj16, a 16 k Da protein derived from Schistosoma japonicum, has been identified as an immune modulation molecule, but the mechanisms of modulation of immune responses are poor known. In this report, we want to reveal the immunity mechanism of regulation of host immune responses by r Sj16 and illuminate the molecule mechanism of immune evasion of Schistosoma japonicum, Methods: Purified r Sj16 protein was produced from E.coil. Using the antibody blocking experiments and knockout mouse model, the effects of r Sj16 and r Sj16 mutants on the biology of dendritic cells were assessed by examining DC maturation, cytokine production and expression of surface markers by flow cytometry and ELISA. Statistical significance was determined by Two-way ANOVA. Results: We investigated the influence of r Sj16 on LPS-induced dendritic cells and found that r Sj16 could significantly stimulate IL-10 production and inhibit LPS-induced BMDC maturation in a dose-dependent manner. Using antibody neutralization experiment and IL-10-deficent(IL-10 Knockout) mice, we further confirmed that the inhibitory effect of r Sj16 on LPS-induced BMDCs is due to its induction of IL-10 production. To understand how r Sj16 induce the production of IL-10, we analyzed the sequence and revealed two potential nuclear localization signal(NLS) of Sj16. Further studies showed that N-terminal NLS(NLS1) is both necessary and sufficient for translocation of r Sj16 to the BMDC’s nucleus and important for subsequent induction of IL-10 production and inhibition of BMDCs maturation by r Sj16. Conclusion: Our results suggest that r Sj16 enter into the nuclear of host cells using the NLS1 and promote the production of IL-10 which mediates the inhibitory effect of r Sj16 on LPS-induced BMDCS.

关键词： dendritic cell rSj16 immuno-modulation nuclear localization signal IL-10

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载多西紫杉醇Poloxamer188复合PEG100k电纺纤维的表征

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锦州医科大学学报 2024年第2期45卷 9-17页

作者：邱志越王瑛颖吕蕴麒王浩锦州医科大学药学院辽宁锦州121000

目的考察Poloxamer188复合PEG100k电纺纤维的形态及载多西紫杉醇后的形态、材料复合,以及载药纤维水化后药物增溶和包裹情况。方法将多西紫杉醇、Poloxamer188和PEG100k溶解在二氯甲烷中形成共溶液后静电纺丝。考察了Poloxamer188的复... 详细信息

目的考察Poloxamer188复合PEG100k电纺纤维的形态及载多西紫杉醇后的形态、材料复合,以及载药纤维水化后药物增溶和包裹情况。方法将多西紫杉醇、Poloxamer188和PEG100k溶解在二氯甲烷中形成共溶液后静电纺丝。考察了Poloxamer188的复合量从10%直至100%以及多西紫杉醇载量为5%、10%、15%、20%等情况。扫描电子显微镜考察纤维形态、差示热分析、热重分析和X-射线衍射考察材料复合及载药。分别在纯水/载药纤维为40/100和5/100(V/m,mL/mg)两种水化条件下水化载药纤维,前者得到液体后者溶胀。在10000 r/min下离心3 min后,前者得到固体沉淀和上清液,后者得到水凝胶沉淀和上清液。采用高效液相色谱-紫外分光光度法测定两种不同处理方法下上清液中多西紫杉醇的含量,分别计算药物增溶百分率和渗漏百分率。结果所得电纺纤维直径均小于5μm,直径较为均一且完全无珠子结构存在。物质之间发生复合,但是在纤维表面有PEG100k片段的衍射峰。水化后所得的液体,5%多西紫杉醇载量的纤维药物增溶百分率最高,大部分样品在70%~80%之间,载药量升高至10%、15%时,增溶百分率降至20%以下,载药量升高至20%时,大部分样品增溶量接近于0。5%载药量系列纤维随着Poloxamer188复合量的增高药物增溶百分率先升高后降低,F-5%-40%中多西紫杉醇的增溶百分率可达80%。多西紫杉醇载量为5%时,水化后所得的凝胶渗漏百分率大于其他载药量的情况,但是未超过5%的渗漏百分率,载药量为10%、15%、20%时,渗漏百分率均小于2%。结论通过均相溶液静电纺丝技术可以制备形态较为理想的载多西紫杉醇PEG100k纤维,水化后纤维中的药物增溶量可以达到药典标准,多西紫杉醇载量及Poloxamer188复合量对药物增溶量影响明显,且所得水化凝胶渗漏率很低。

关键词：多西紫杉醇电纺纤维聚乙二醇泊洛沙姆增溶

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基础医学拔尖创新人才培养中的本科生导师制

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医学教育管理 2022年第3期8卷 265-269页

作者：王淑珍许志威朱毅琼丁晓艺曹素贞吕志跃中山大学中山医学院广州510080

中山医学院在基础医学专业实施本科生双导师制已有很多年。双导师制指第二三学年的全程导师制度,第四五学年的科研导师制。全程导师对学生的专业思想、专业学习、科研培养和就业规划等给予指导和帮助,培养学生初步科研思维,训练基础科... 详细信息

中山医学院在基础医学专业实施本科生双导师制已有很多年。双导师制指第二三学年的全程导师制度,第四五学年的科研导师制。全程导师对学生的专业思想、专业学习、科研培养和就业规划等给予指导和帮助,培养学生初步科研思维,训练基础科研技术,培养文献综述能力等;科研导师将负责学生毕业论文设计指导、课题实施、论文答辩等。学院通过定期遴选优秀导师,培训导师,如今已形成一支优质的导师队伍,通过组织导师学生见面会,双导师各有侧重,互相配合,鼓励学生早进入导师团队、早进入实验室、早进入科研项目等措施,学生的科研兴趣、创新能力得到明显提升,荣获多项国家级学生科研奖励,双导师制得到了学院师生的一致好评。早期接触科研是中山医学院医学教育“三早”特色中的一个重要组成,导师制也成为我校本科教育教学阶段的一个重要品牌。在2020年我们获得基础医学专业首批强基计划项目后,这一传统更是得到了进一步的发展,并固化为基础医学专业人才培养方案中的内容,成为助推基础医学强基计划实施的有力举措之一。本文重点介绍在基础医学学科,结合强基计划与拔尖人才培养的要求,如何进一步开展本科生导师制。

关键词：基础医学拔尖创新人才培养本科生导师制

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我国医学院校人体寄生虫学课程建设面临的问题与挑战

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基础医学教育 2018年第1期20卷 1-6页

作者：刘明社孙希吕志跃吴忠道长治医学院人体寄生虫学教研室长治046000 中山大学中山医学院人体寄生虫学教研室热带病防治研究教育部重点实验室

文章调查了中国大陆的64所高等医药院校寄生虫学课程的设置情况;评估了课程设置就寄生虫学课程对医学生培养可能带来的影响,指出课程设置中存在的学时偏少、重视不够的问题,对于国家"一带一路"发展的影响以及对新的输入性寄生虫病的出... 详细信息

文章调查了中国大陆的64所高等医药院校寄生虫学课程的设置情况;评估了课程设置就寄生虫学课程对医学生培养可能带来的影响,指出课程设置中存在的学时偏少、重视不够的问题,对于国家"一带一路"发展的影响以及对新的输入性寄生虫病的出现和危害带来的挑战,为医学教育模式改革和完善人体寄生虫学课程建设提供了第一手证据资料。

关键词：人体寄生虫学课程设置教学内容

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小麦辣椒玉米套种的效应与栽培技术初报

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中国农学通报 2006年第3期22卷 211-213页

作者：王周录吕广林王志岳宝鸡市农技中心陕西宝鸡721001

陕西利用生物学互助的原理,研究成功麦、辣、玉三作物按4～5:2:0.5的行数比梯阶套种模式,既构建成功复合群体良好生育环境,又增收粮食4900～5200kg/hm2,辣椒干750kg/hm2,节约防病虫投入900～1200元/hm2,同时提出高效节本套种的五项关键... 详细信息

陕西利用生物学互助的原理,研究成功麦、辣、玉三作物按4～5:2:0.5的行数比梯阶套种模式,既构建成功复合群体良好生育环境,又增收粮食4900～5200kg/hm2,辣椒干750kg/hm2,节约防病虫投入900～1200元/hm2,同时提出高效节本套种的五项关键栽培技术。

关键词：辣椒小麦玉米套种效应栽培

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基于相机移动的鱼眼成像系统目标方位标定研究

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激光杂志 2020年第9期41卷 24-28页

作者：肖智越刘力双吕勇孙鹏北京信息科技大学仪器科学与光电工程学院北京100192

针对大视场鱼眼成像系统中需要对目标方位与像素之间关系进行标定的问题,提出一种基于相机移动的标定方法。标定物为单个LED光源,通过最小二乘曲线拟合法定位其光点的中心。控制电动高精度二维旋转云台带动鱼眼成像系统相对标定物做旋... 详细信息

针对大视场鱼眼成像系统中需要对目标方位与像素之间关系进行标定的问题,提出一种基于相机移动的标定方法。标定物为单个LED光源,通过最小二乘曲线拟合法定位其光点的中心。控制电动高精度二维旋转云台带动鱼眼成像系统相对标定物做旋转运动,拍摄标定物图像,获得旋转云台姿态角与标定点像素坐标的对应点数据集。采用两步法的标定模型,先后进行光学中心计算、线性模型标定和畸变参数标定。结合大视场的特点,采用分区域标定的方式来减小非中心区域成像时的标定精度误差。实验结果表明:该标定方法具有较高的标定精度,可应用于航空、航天等大视场环境下的鱼眼成像系统标定。

关键词：鱼眼成像系统标定畸变光学中心相机移动

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