检索结果-南通市图书馆

大豆斑疹病菌铁摄取因子PiuB在致病性中的作用分析

在线全文

学校读者我要写书评

暂无评论

生物工程学报 2024年第1期40卷 177-189页

作者：苏如意金罗佳徐江玲耿慧雅陈晓林思怡郭威纪志远浙江师范大学生命科学学院浙江金华321004 中国农业科学院作物科学研究所农作物基因资源与基因改良国家重大科学工程北京100081

铁作为生命必需的基本元素,在细菌生长代谢过程中具有重要作用。然而,大豆斑疹病菌(Xanthomonas axonopodis ***,Xag)中编码铁摄取因子的piuB基因是否参与病原菌的铁摄取和致病性并不清楚。为解析PiuB的作用,采用同源重组策略获得了Xag... 详细信息

铁作为生命必需的基本元素,在细菌生长代谢过程中具有重要作用。然而,大豆斑疹病菌(Xanthomonas axonopodis ***,Xag)中编码铁摄取因子的piuB基因是否参与病原菌的铁摄取和致病性并不清楚。为解析PiuB的作用,采用同源重组策略获得了Xag的piuB基因缺失突变株(ΔpiuB),并对该突变株进行功能研究。研究表明:相较于野生型,突变株ΔpiuB在寄主大豆上的毒性和生长能力显著削弱;铁载体分泌量激增;对Fe^(3+)、Cu^(2+)、Zn^(2+)和Mn^(2+)的敏感性显著增强。此外,该突变株的H_(2)O_(2)抗性、胞外多糖产量、生物膜形成能力以及游动性等相较于野生型均显著减弱;添加外源Fe^(3+)不能有效恢复ΔpiuB的上述特性;功能互补株可完全恢复ΔpiuB的缺陷性表型至野生型水平。这说明PiuB是Xag摄取Fe^(3+)的潜在因子,并且是Xag在寄主大豆上致病所需的。

关键词：大豆斑疹病菌铁摄取因子PiuB Fe^(3+) 致病性

在线全文

学校读者我要写书评

暂无评论

突变结构对π型管气流脉动的影响

化工设备与管道 2024年第4期61卷 56-63页

作者：谭红莹刘健郎子进纪志远司红涛单世超周波海洋石油工程股份有限公司设计院天津300461 大连理工大学工程工业装备结构分析优化与CAE软件国家重点实验室船舶工程学院辽宁大连116024

世界能源发展呈现低碳趋势,人们对天然气的需求日益增加。输运管道中由于气流脉动遇到弯头、变径管等突变结构而形成的激振力是管道振动的主要成因。π型管是天然气管道中常见的柔性补偿结构,常与压缩机出口的管路相连,往复式压缩机工... 详细信息

世界能源发展呈现低碳趋势,人们对天然气的需求日益增加。输运管道中由于气流脉动遇到弯头、变径管等突变结构而形成的激振力是管道振动的主要成因。π型管是天然气管道中常见的柔性补偿结构,常与压缩机出口的管路相连,往复式压缩机工作时产生的气流脉动在π型管处诱发的流致振动较为明显。文章基于计算流体动力学对含有突变结构的π型管进行气流脉动仿真分析,研究脉动幅值与突变结构的关系。结果显示,含突变结构的管道出口处的压力不均匀度比π型管分别高了3.8%和4.6%左右,当突变结构的尺寸从100 mm变化到200 mm时,管道出口处的压力不均匀度也从15.5%左右变化到19.9%左右,可以看出突变结构对π型管内部的气流脉动呈现出不利影响,在实际工程应用中应尽量避免突变结构的使用。

关键词：气流脉动流致振动突变结构减振

Xa1 Allelic R Genes Activate Rice Blight Resistance Suppressed by Interfering TAL Effectors

在线全文

学校读者我要写书评

暂无评论

Plant Communications 2020年第4期1卷 54-62页

作者： Chonghui ji zhiyuan ji Bo Liu He Cheng Hua Liu Sanzhen Liu Bing Yang Gongyou Chen Division of Plant Sciences C.Bond Life Science CenterUniversity of MissouriColumbiaMO 65211USA School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism Shanghai Jiao Tong UniversityShanghai 200240P.R.China National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Department of Plant Pathology Kansas State UniversityManhattanKS 66506USA Donald Danforth Plant Science Center St.LouisMO 63132USA

Xanthomonas oryzae pathovar oryzae(Xoo)uses transcription activator-like effectors(TALEs)to cause bacterial blight(BB)in *** turn,rice has evolved several mechanisms to resist BB by targeting *** mechanism involves the nucleotide-binding leucine-rich repeat(NLR)resistance gene Xa1 and ***,Xoo has evolved TALE variants,C-terminally truncated versions(interfering TALEs or iTALEs),to overcome Xa1 ***,it remains unknown to what extent the two co-adaptive mechanisms mediate Xoo–rice *** this study,we cloned and characterized five additional Xa1 allelic R genes,Xa2,Xa31(t),Xa14,CGS-Xo111,and Xa45(t)from a collection of rice *** analysis revealed that Xa2 and Xa31(t)from different rice cultivars are *** genes and their predicted proteins were found to be highly conserved,forming a group of Xa1 *** XA1 alleles could be distinguished by the number of C-terminal tandemrepeats consisting of 93 amino acid residues and ranged from four in XA14 to seven in XA45(t).Xa1 allelic genes were identified in the 3000 rice genomes *** the other hand,iTALEs could suppress the resistance mediated by Xa1 allelic R genes,and iTALE genes were prevalent(95%)in Asian,but not in African Xoo *** findings demonstrate the prominence of a defense mechanism in which rice depends on Xa1 alleles and a counteracting mechanismin which Xoo relies on iTALEs for BB.

关键词： Xa2 Xa14 Xa45(t) TAL effector iTAL effector bacterial blight

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

水稻抗细菌性条斑病基因挖掘与初定位

植物学报 2024年第1期59卷 1-9页

作者：方妍力田传玉苏如意刘亚培王春连陈析丰郭威纪志远浙江师范大学生命科学学院金华321004 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程北京100081

由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv. oryzicola,Xoc)引起的细菌性条斑病(BLS)是水稻(Oryza sativa)生产上的重要病害,近年来其发病呈快速上升趋势,特别是在我国南方(包括江苏、浙江、福建和广东等地)稻区危害严重。种植... 详细信息

由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv. oryzicola,Xoc)引起的细菌性条斑病(BLS)是水稻(Oryza sativa)生产上的重要病害,近年来其发病呈快速上升趋势,特别是在我国南方(包括江苏、浙江、福建和广东等地)稻区危害严重。种植抗病品种是防治BLS最理想的措施,但目前生产上严重缺乏可用于育种的优异抗病基因资源。通过人工接种鉴定筛选水稻种质资源,挖掘到2份高抗BLS材料(M1和D1)。多菌株系接种结果表明,M1具有非小种特异性广谱抗病(RNS BSR)特征。经遗传群体分析表明,栽培稻M1携带单个显性抗BLS新基因Xo-3。通过混池测序和关联分析,将Xo-3基因初步定位在2号染色体上的一段候选区域内。抗BLS种质资源的挖掘及其抗性遗传基础的解析,将有助于理解水稻-Xoc互作机理,从而培育抗BLS水稻新品种和制定科学的防治BLS策略。

关键词：细菌性条斑病种质资源抗病基因水稻稻黄单胞菌

Crystallization of RE_(2)(OH)_(2)CO_(3)SO_(4)·nH_(2)O as a new family of layered hydroxides(RE=Gd-Lu lanthanides and Y),derivation of RE_(2)O_(2)SO_(4),photoluminescence and optical thermometry

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2024年第8期42卷 1496-1506,I0003页

作者： Fan Li Zhenqi Song zhiyuan Pan Sihan Feng Qi Zhu ji-Guang Li Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education) School of Materials Science and EngineeringNortheastern UniversityShenyang110819China Research Center for Functional Materials National Institute for Materials ScienceTSsukuboaIbaraki3050044Japan

Layered rare-earth hydroxides(LREHs) draw wide research interest because of their peculiar crystal structure,rich interlayer chemistry and abundant functionality of the RE element,but are limited to the two categories of RE_(2)(OH)_(5)A·nH_(2)O(A:typical of Cl^(-)or NO_(3)^(-)) and RE_(2)(OH)_(4)SO_(4)·nH_(2)*** the other hand,rare-earth oxysulfates(RE_(2)O_(2)SO_(4)) have attracted attention due to their properties of large-capacity oxygen storage,low-temperature magnetism and luminescence,but their preparation procedure mostly involves toxic SO_(x) gases and/or complicated *** this work,RE_(2)(OH)_(2)CO_(3)SO_(4)·nH_(2)O as a new family of LREHs(RE=Gd-Lu lanthanides and Y) were produced via hydrothermal reaction,from which phase-pure RE_(2)O_(2)SO_(4) was derived via subsequent annealing at 800℃ in air without the involvement of SO_(x),The compounds were thoroughly characterized to reveal the intrinsic influence of lanthanide contraction(RE^(3+) radius) on crystal structure,thermal behavior(dehydroxylation/decarbonation/desulfurization),vibrational property and crystallite *** analyzing the photoluminescence of Eu^(3+) and Sm^(3+)in the Gd_(2)O_(2)SO_(4) typical host it is found that the 617 nm(Eu^(3+),λ_(ex)=275 nm) and 608 nm(Sm^(3+),λ_(ex)=407 nm) main emissions can retain as high as ^(7)9.6% and 85.5%of their room-temperature intensities at 423 K,with activation energies of ~0.19 and 0.21 eV for thermal quenching,*** also indicates that both the phosphors have the potential for optical temperature sensing via the fluorescence intensity ratio(FIR) technology,whose maximum relative sensitivity reaches -2.70%/K for Eu^(3+)and 1.73%/K for Sm^(3+) at 298 K.

关键词： Layered hydroxide RE_(2)O_(2)SO_(4) Luminescence Optical temperature sensing Rare earths

A Potential Disease Susceptibility Gene CsLOB of Citrus Is Targeted by a Major Virulence Effector PthA of Xanthomonas citri subsp, citri

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2014年第5期7卷 912-915页

作者： Zheng Li Lifang Zou Gang Ye Li Xiong zhiyuan ji Muhammad Zakria Ni Hong Guoping Wang Gongyou Chen School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China State Key Laboratory of Microbial Metabolism School of Life Science &Biotechnology Shanghai Jiao Tong University Shanghai 200240 China National Key Laboratory of Agromicrobiology Huazhong Agricultural University Wuhan Hubei 430070 China

Dear Editor, Citrus bacterial canker （CBC）, one of the most destructive diseases of citrus, has a severe economic impact on citrus industries in tropical and subtropical countries （Das, 2003）. It has been widely known that Xanthomonas citri subsp, citri （Xcc） causes the CBC by transferring the type-Ill effectors （T3SEs） to the interior of the plant cell through type-Ill secretion system （T3S） to modulate transcription of host plants to trigger CBC disease （Schaad et al., 2006）. Pathotype A,

关键词： CITRUS canker CITRUS fruit industry NUCLEOTIDES PATHOGENIC microorganisms MUTAGENESIS

Engineering Broad-Spectrum Resistance to Bacterial Blight by CRISPR/Cas9-Mediated Precise Homology Directed Repair in Rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2021年第8期14卷 1215-1218页

作者： Zheng Wei Mohamed Abdelrahman Ying Gao zhiyuan ji Rukmini Mishra Hongda Sun Yi Sui Chuanyin Wu Chunlian Wang Kaijun Zhao National Key Facility for-Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijingChina Rice Research and Training Center Field Crops Research InstituteAgricultural Research CenterKafrelsheikhEgypt

Dear Editor,Rice(Oryza sativa)is one of the most important food crops as well as a model plant for basic *** bacterial blight(BB)caused by Xanthomonas oryzae ***(Xoo)is a devastating bacterial disease worldwide(Mew et al.,1993),affecting millions of hectares of rice annually,with an estimated crop loss of as high as 75%.Upon invasion of rice cells,Xoo secretes transcription activator-like effectors(TALEs)to activate the host Sugars Will Eventually be Exported Transporters(SWEET)genes to obtain nutrition for bacterial growth,because the induced SWEET transports more sugars to where the bacteria reside(Boch and Bonas,2010).

关键词： sativa CRISPR/Cas9 blight

Spatial transcriptomics reveals that metabolic characteristics define the tumor immunosuppression microenvironment via iCAF transformation in oral squamous cell carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Oral Science 2024年第1期16卷 110-121页

作者： Zheqi Liu Zhen Zhang Yu Zhang Wenkai Zhou Xu Zhang Canbang Peng Tong ji Xin Zou zhiyuan Zhang Zhenhu Ren Department of Oral and Maxillofacial-Head and Neck Oncology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghaiChina Department of Oral and Maxillofacial Surgery Zhongshan HospitalFudan UniversityShanghaiChina School and Hospital of Stomatology Kunming Medical UniversityKunmingChina Center for Tumor Diagnosis&Therapy Jinshan HospitalFudan UniversityShanghaiChina Department of Pathology Jinshan HospitalFudan UniversityShanghaiChina Hainan Western Central Hospital Academician Zhang Zhiyuan Team Innovation CenterDanzhouChina

Tumor progression is closely related to tumor tissue metabolism and reshaping of the microenvironment. Oral squamous cell carcinoma (OSCC), a representative hypoxic tumor, has a heterogeneous internal metabolic environment. To clarify the relationship between different metabolic regions and the tumor immune microenvironment (TME) in OSCC, Single cell (SC) and spatial transcriptomics (ST) sequencing of OSCC tissues were performed. The proportion of TME in the ST data was obtained through SPOTlight deconvolution using SC and GSE103322 data. The metabolic activity of each spot was calculated using scMetabolism,and k-means clustering was used to classify all spots into hyper-, normal-, or hypometabolic regions. CD4T cell infiltration and TGF-βexpression is higher in the hypermetabolic regions than in the others. Through CellPhoneDB and NicheNet cell-cell communication analysis, it was found that in the hypermetabolic region, fibroblasts can utilize the lactate produced by glycolysis of epithelial cells to transform into inflammatory cancer-associated fibroblasts (iCAFs), and the increased expression of HIF1A in iCAFs promotes the transcriptional expression of CXCL12. The secretion of CXCL12 recruits regulatory T cells (Tregs), leading to Treg infiltration and increased TGF-β secretion in the microenvironment and promotes the formation of a tumor immunosuppressive microenvironment. This study delineates the coordinate work axis of epithelial cells-iCAFs-Tregs in OSCC using SC, ST and TCGA bulk data, and highlights potential targets for therapy.

关键词： CXCL12 microenvironment squamous

An Aerial–Wall Robotic Insect That Can Land, Climb, and Take Off from Vertical Surfaces

在线全文

学校读者我要写书评

暂无评论

Research 2024年第1期2023卷 149-163页

作者： Qian Li Haoze Li Huan Shen Yangguang Yu Haoran He Xincheng Feng Yi Sun zhiyuan Mao Guangming Chen Zongjun Tian Lida Shen Xiangming Zheng Aihong ji College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjingChina College of Aerospace Engineering Nanjing University of Aeronautics and AstronauticsNanjingChina State Key Laboratory of Mechanics and Control for Aerospace Structures Nanjing University of Aeronautics and AstronauticsNanjingChina

Insects that can perform flapping-wing flight,climb on a wall,and switch smoothly between the 2 locomotion regimes provide us with excellent biomimetic ***,very few biomimetic robots can perform complex locomotion tasks that combine the 2 abilities of climbing and ***,we describe an aerial–wall amphibious robot that is self-contained for flying and climbing,and that can seamlessly move between the air and *** adopts a flapping/rotor hybrid power layout,which realizes not only efficient and controllable flight in the air but also attachment to,and climbing on,the vertical wall through a synergistic combination of the aerodynamic negative pressure adsorption of the rotor power and a climbing mechanism with bionic adhesion *** the basis of the attachment mechanism of insect foot pads,the prepared biomimetic adhesive materials of the robot can be applied to various types of wall surfaces to achieve stable *** longitudinal axis layout design of the rotor dynamics and control strategy realize a unique cross-domain movement during the flying–climbing transition,which has important implications in understanding the takeoff and landing of ***,it enables the robot to cross the air–wall boundary in 0.4 s(landing),and cross the wall–air boundary in 0.7 s(taking off).The aerial–wall amphibious robot expands the working space of traditional flying and climbing robots,which can pave the way for future robots that can perform autonomous visual monitoring,human search and rescue,and tracking tasks in complex air–wall environments.

关键词： robot smoothly Vertical