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The Crop Journal 2014年第Z1期2卷 164-169页

作者： Baoqing Dun Xujing wang Wei Lu Ming Chen Wei Zhang Shuzhen Ping zhixing wang Baoming Zhang Min Lin Institute of Crop Sciences The National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural Sciences Biotechnology Research Institute Chinese Academy of Agricultural Sciences

The widely used herbicide glyphosate targets 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS).Glyphosate acetyltransferase(GAT)effectively detoxifies glyphosate by *** the aim of identifying a new strategy for development of glyphosate-tolerant crops,the plant expression vector pG2-GAT harboring gat and G2-aroA(encoding EPSPS)has been transformed into tobacco(Nicotiana tabacum)to develop novel plants with higher tolerance to *** from Southern and Western blotting analyses indicated that the target genes were integrated into tobacco chromosomes and expressed effectively at the protein *** tolerance was compared among transgenic tobacco plants containing gat,G2-aroA,or both *** containing both gat and G2-aroA genes were the most *** study has shown that a combination of different strategies may result in higher tolerance in transgenic crops,providing a new approach for development of glyphosate-tolerant crops.

关键词： Transgenic tobacco Glyphosate tolerance gat gene G2-aroA gene

Cooperation of nitrogen-doping and catalysis to improve the Li-ion storage performance of lignin-based hard carbon

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Journal of Energy Chemistry 2018年第5期27卷 1390-1396页

作者： Zhewei Yang Huajun Guo Feifei Li Xinhai Li zhixing wang Lizhi Cui Jiexi wang School of Metallurgy and Environment Central South University

Hard carbon draws great interests as anode material in lithium ion batteries （LIBs） due to its high the- oretical capacity, high rate capability and abundance of its precursors. Herein we firstly synthesize the lignin-melamine resins by grafting melamine onto lignin. Afterwards, nitrogen doped hard carbon is pre- pared by the pyrolysis of lignin-melamine resins with the aid of catalyst （Ni（NO_3）2·6H_2O） at 1000 ℃. Compared with the samples without nitrogen-doping and catalysis, as-prepared nitrogen doped hard car- bon exhibits higher reversible capacity （345 mAh g-1 at 0.1 A g-1 ）, higher rate capability （145 mAh g-1 at 5 A g-1） and excellent cycling stability. The superior electrochemical performance is ascribed to the synergistic effect of nitrogen doping, graphitic structure and amorphous structure. Among them, nitro- gen doping could create the vacancies around the nitrogen sites, which enhance the reactivity and the electronic conductivity of materials. Additionally, graphitic structure also enhances the electronic con- ductivity of materials, thus improving the electrochemical performance of hard carbon. It is worthwhile that Iignin, renewable and abundant biopolymer, is converted to hard carbon with good electrochemical performance, which realizes the high value utilization of lignin.

关键词： Hard carbon Lignin Melamine Nitrogen-doping Catalysis

The tumor-enriched small molecule gambogic amide suppresses glioma by targeting WDR1-dependent cytoskeleton remodeling

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Signal Transduction and Targeted Therapy 2023年第12期8卷 5904-5918页

作者： Jiaorong Qu Bojun Qiu Yuxin Zhang Yan Hu zhixing wang Zhiang Guan Yiming Qin Tongtong Sui Fan Wu Boyang Li Wei Han Xiaozhong Peng Department of Molecular Biology and Biochemistry Institute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijing 100005China State Key Laboratory of Common Mechanism Research for Major Diseases BeijingChina Department of Molecular Neuropathology Beijing Neurosurgical InstituteCapital Medical UniversityBeijing 100070China Department of Neurosurgery Beijing Tiantan HospitalCapital Medical UniversityBeijingChina State Key Laboratory of Respiratory Health and Multimorbidity BeijingChina National Human Diseases Animal Model Resource Center Beijing Engineering Research Center for Experimental Animal Models of Human Critical DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China

Glioma is the most prevalent brain tumor,presenting with limited treatment options,while patients with malignant glioma and glioblastoma(GBM)have poor *** physical obstacle to drug delivery imposed by the blood‒brain barrier(BBB)and glioma stem cells(GSCs),which are widely recognized as crucial elements contributing to the unsatisfactory clinical *** this study,we found a small molecule,gambogic amide(GA-amide),exhibited the ability to effectively penetrate the blood-brain barrier(BBB)and displayed a notable enrichment within the tumor ***,GA-amide exhibited significant efficacy in inhibiting tumor growth across various in vivo glioma models,encompassing transgenic and primary patient-derived xenograft(PDX)*** further performed a genome-wide clustered regularly interspaced short palindromic repeats(CRISPR)knockout screen to determine the druggable target of *** the combination of the cellular thermal shift assay(CETSA),the drug affinity responsive target stability(DARTS)approach,molecular docking simulation and surface plasmon resonance(SPR)analysis,WD repeat domain 1(WDR1)was identified as the direct binding target of *** direct interaction with WDR1,GA-amide promoted the formation of a complex involving WDR1,MYH9 and Cofilin,which accelerate the depolymerization of F-actin to inhibit the invasion of patient-derived glioma cells(PDCs)and induce PDC apoptosis via the mitochondrial apoptotic *** conclusion,our study not only identified GA-amide as an effective and safe agent for treating glioma but also shed light on the underlying mechanisms of GA-amide from the perspective of cytoskeletal homeostasis.

关键词： glioma invasion homeostasis

AN EXPLOITATION OF THE EXPERT SYSTEM OF MECHANICAL DESIGN BASED ON VIRTUAL REALITY

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Chinese Journal of Mechanical Engineering 1999年第2期12卷 69-72页

作者： Wu Qunbo wang zhixing Li Chengyu Guan Liwen Dept of Mechanical Engineering, Harbin Institute of Technology 哈尔滨工业大学机械工程系黑龙江哈尔滨 150001 哈尔滨工业大学机械工程系黑龙江哈尔滨

Object oriented methodology is introduced to build the model on expert system of mechanical design. The combination of knowledge representation with object oriented method and neural network make the expression of the complex knowledge in mechanical design world reasonably and properly, and the complicated system is built successfully. Also, the merging of virtual reality art and conventional mechanical design theory puts visual three dimensional mechanical movement into realities.

关键词： Virtual reality Expert system Neural networks

Theoretical Basis of Gene Splitting Technique and Its Application in the Control of Transgene Flow

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Agricultural Biotechnology 2015年第3期4卷 1-4页

作者： Yufeng DONG Xujing wang Qiaoling TANG zhixing wang Biotechnology Research Institute Chinese Academy of Agricultural Sciences

Transgenic safety issues cause more and more controversies with the planting area of transgenie crops increased year by year. Gene flow from transgenie crops to wild relatives through pollen dispersal is one of the focus problems. Gene splitting technique provides a new strategy for the control of transgene flow by bio-logical containment. The construction of gene splitting technique is based on protein trans-splicing mediated by intein. Currently, it has been proved in Arabidopsis, tabaoco, wheat, etc. that active and functional proteins can be reassembled by intein mediated protein trans-splicing after gene splitting, which provides theoretical basis and experimental supporting for the limit of transgene flow by gene splitting technique. The theoretical basis of gene splitting technique and research progresses of its application on the control of transgene flow were reviewed in this paper.

关键词： Gene splitting Intein Gene flow

七轴混联机床机构干涉校验算法及工作空间快速校验的研究

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机械工程学报 2006年第2期42卷 221-225页

作者：王瑞王知行钟诗胜哈尔滨工业大学机电工程学院哈尔滨150001

在加工具有复杂曲面的工件时,同六轴并联机床相比,七轴混联机床的加工能力有所提高,能够快速加工多个曲面,降低由于多次装夹造成的加工误差,缩短加工时间,有利于充分发挥并联机床的优势。为了避免加工过程中出现干涉碰撞问题,同时确保... 详细信息

在加工具有复杂曲面的工件时,同六轴并联机床相比,七轴混联机床的加工能力有所提高,能够快速加工多个曲面,降低由于多次装夹造成的加工误差,缩短加工时间,有利于充分发挥并联机床的优势。为了避免加工过程中出现干涉碰撞问题,同时确保整个工件都在加工空间内,提出并详细讨论了七轴混联机床的机构干涉校验算法及工作空间快速校验算法。这些算法对混联机床的设计和控制系统的开发有着一定意义。

关键词：混联机床干涉校验工作空间快速校验

刀具扫描体生成新算法及在数控加工仿真中的应用

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机械工程学报 2001年第1期37卷 28-31页

作者：王哲王知行钟诗胜哈尔滨工业大学机电学院哈尔滨150001

介绍一种新的刀具扫描体生成算法。通过将运动物体外表面上的点分成入点、出点和切点 ,新算法能够迅速找到某一时刻运动物体外表面上的切点而形成扫描体 ,从而不依赖于以往扫描体生成的包络法 ,避免求解复杂的方程 ,因而具有方法简单 ,... 详细信息

介绍一种新的刀具扫描体生成算法。通过将运动物体外表面上的点分成入点、出点和切点 ,新算法能够迅速找到某一时刻运动物体外表面上的切点而形成扫描体 ,从而不依赖于以往扫描体生成的包络法 ,避免求解复杂的方程 ,因而具有方法简单 ,编程容易 ,运算速度快等优点。虽然该算法是针对刀具扫描体生成开发的 ,但它适用于任何回转体扫描体的形成。

关键词：扫描体算法数控加工仿真

Physicochemical properties of a novel composite polymer electrolyte doped with vinyltrimethoxylsilane-modified nano-La_2O_3

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Journal of Rare Earths 2012年第10期30卷 1034-1040页

作者：肖围李新海王志兴郭华军王接喜黄思林甘雷 School of Metallurgical Science and Engineering Central South University

Nano-La2O3 was modified with the vinyltrimethoxylsilane by hydrolysis and a novel poly （vinylidene fluoride-co-hexafluoropropylene）（PVDF-HFP） based composite polymer electrolyte doped with the modified nano-La2O3 was prepared by phase inversion method. The physicochemical properties were studied by SEM, FT-IR, XRD, TG and electrochemical methods. The results of FT-IR indicated that the nano-La2O3 was successfully modified with vinyltrimethoxylsilane. The XRD analysis showed that the incorporation of modified nano-La2O3 into the polymer electrolyte membranes could effectively reduce the crystallinity of PVDF-HFP, and the characterizations also suggested that thermal stability and electrochemical stability window could reach to 382°C and 5.1V, respectively; the reciprocal temperature dependence of ionic conductivity followed Vogel-Tamman-Fulcher （VTF） relation, ionic conductivity at room temperature was up to 3.5×10-3S/cm and lithium ions transference number was up to 0.42; the interfacial resistance increased at initial value about353Ω/cm2 and reached a steady value about 559Ω/cm2 after 5d storage at 30°C. The fabricated Li/As-prepared electrolytes/LiCoO2 coin cell showed excellent rate and cycle performances.

关键词： composite polymer electrolyte vinyltrimethoxylsilane modify nano-La2O3 rare earths

WORKPIECE LOCATING AND POST PROCESSING SYSTEMS ON 6-DOF CNC MILLING MACHINE

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Transactions of Nanjing University of Aeronautics and Astronautics 2006年第2期23卷 138-143页

作者：王瑞钟诗胜王知行哈尔滨工业大学机电工程学院

A conventional non-computerized numerical control （CNC） machine is updated by mounting a six degree-of-free （DOF） parallel mechanism on it, thus obtaining a new CNC one. The structure of this CNC milling machine is introduced, and the workpiece locating system and the post processing system of the cutter location （CL） data file are analyzed. The new machine has advantages of low costs, simple structure, good rigidity, and high precision. It is easy to be transformed and used to process the workpiece with a complex surface.

关键词： parallel kinematic machine CNC milling machine workpiece locating system post processing system

新型2-TPR/2-TPS空间4自由度并联机构

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机械工程学报 2008年第11期44卷 298-303页

作者：伞红军钟诗胜王知行哈尔滨工业大学机电工程学院哈尔滨150001

提出一种新型2-TPR/2-TPS空间4自由度并联机构。机构的运动平台通过两个相邻的TPR分支运动链和两个相邻的TPS分支运动链与固定平台相联接。每个TPR分支由一个胡克铰T、一个移动副P和一个转动副R组成,一端通过胡克铰T与固定平台相联,另... 详细信息

提出一种新型2-TPR/2-TPS空间4自由度并联机构。机构的运动平台通过两个相邻的TPR分支运动链和两个相邻的TPS分支运动链与固定平台相联接。每个TPR分支由一个胡克铰T、一个移动副P和一个转动副R组成,一端通过胡克铰T与固定平台相联,另一端通过转动副R与运动平台相联;每个TPS分支由一个胡克铰T、一个移动副P和一个球面副S组成,一端通过胡克铰T与固定平台相联,另一端通过球面副S与运动平台相联。运用约束螺旋理论,对机构的运动性质和奇异位形进行分析。机构运动平台具有沿两个TPR分支所在平面的二维移动自由度,和以两个TPR分支胡克铰二级转动副轴线所在平面内的任何直线作为转轴的二维转动自由度。选取4个分支运动链的移动副作为驱动副,发现了机构可能出现的四种奇异位形。

关键词：并联机构螺旋理论 4自由度运动性质奇异位形