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温度对过氧化氢抑制前成骨细胞MC3T3-E1细胞增殖和成骨分化的影响

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新乡医学院学报 2024年第2期41卷 109-114页

作者：耿卢婧孙智欣李俞辰张瑜史培培新乡医学院生命科学技术学院河南新乡453003

目的探讨温度对过氧化氢(H_(2)O_(2))抑制前成骨细胞增殖和成骨分化的影响。方法取对数生长期MC3T3-E1细胞,随机分为0、450、500、550、600、650μmol·L^(-1)H_(2)O_(2)干预组,分别给予0、450、500、550、600、650μmol·L^(-1)H_(2)O_(2)溶液干预2 h。另取对数生长期MC3T3-E1细胞,随机分为对照组、模型组、低温组和高温组。对照组细胞置于37℃、含体积分数5%CO_(2)培养箱中孵育24 h;模型组细胞置于37℃、含体积分数5%CO_(2)培养箱中孵育24 h,并给予H_(2)O_(2)刺激2 h;低温组细胞置于32℃、含体积分数5%CO_(2)培养箱中孵育24 h,并给予H_(2)O_(2)刺激2 h;高温组细胞置于40℃、含体积分数5%CO_(2)培养箱中孵育24 h,并给予H_(2)O_(2)刺激2 h。采用细胞计数试剂盒-8检测各组细胞增殖能力,实时荧光定量聚合酶链反应法检测细胞中Runt相关转录因子2(RUNX2)、骨桥蛋白(OPN)和骨钙素(OC)mRNA表达水平,Western blot法检测MC3T3-E1细胞中RUNX2、OPN和OC蛋白表达水平。结果0、450、500μmol·L^(-1)H_(2)O_(2)干预组细胞增殖率比较差异无统计学意义(P>0.05);550、600、650μmol·L^(-1)H_(2)O_(2)干预组细胞增殖率显著低于0、450、500μmol·L^(-1)H_(2)O_(2)干预组,且随H_(2)O_(2)浓度增加细胞增殖率显著降低(P0.05)。模型组和高温组细胞中RUNX2 mRNA相对表达量显著高于对照组和低温组,低温组细胞中RUNX2 mRNA相对表达量显著低于对照组(P0.05)。模型组、低温组和高温组细胞中OPN mRNA相对表达量显著高于对照组,低温组和高温组细胞中OPN mRNA相对表达量显著高于模型组,低温组细胞中OPN mRNA相对表达量显著高于高温组(P0.05)。模型组、低温组和高温组细胞中RUNX2、OPN和OC蛋白相对表达量显著低于对照组(P0.05)。高温组细胞中RUNX2、OPN和OC蛋白相对表达量显著高于模型组(P<0.05)。结论H_(2)O_(2)可抑制MC3T3-E1细胞增殖和成骨分化,低温可增强H_(2)O_(2)对MC3T3-E1细胞增殖和成骨分化的抑制作用,高温可缓解H_(2)O_(2)对细胞增殖和成骨分化的抑制作用。RUNX2、OPN和OC蛋白可能在温度调控细胞增殖和成骨分化的过程中发挥重要作用。

关键词：孵育温度氧化损伤成骨细胞 MC3T3-E1细胞细胞增殖成骨分化

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说话人音频攻击与对抗技术研究综述

南京邮电大学学报（自然科学版） 2024年第4期44卷 17-29页

作者：孙知信赵杰王恩良刘晨磊范连成刘畅南京邮电大学江苏省邮政大数据技术与应用工程研究中心江苏南京210003 南京邮电大学国家邮政局邮政行业技术研发中心(物联网技术) 江苏南京210003 南京邮电大学宽带无线通信与传感网技术教育部重点实验室江苏南京210003 安徽南陵县邮政业发展中心安徽芜湖241399

文中概括了说话人音频攻击与对抗技术的最新进展。由于说话人音频攻击已经成为语音应用安全的严重威胁,以WaveNet、Transformer和GAN三种模型在音频攻击技术中的应用作为节点,分别介绍以其为基础的音频攻击技术。音频对抗技术则以涵盖... 详细信息

文中概括了说话人音频攻击与对抗技术的最新进展。由于说话人音频攻击已经成为语音应用安全的严重威胁,以WaveNet、Transformer和GAN三种模型在音频攻击技术中的应用作为节点,分别介绍以其为基础的音频攻击技术。音频对抗技术则以涵盖的攻击技术分为3类,分别是基础音频攻击、重放攻击和深度伪造攻击。系统地阐述了音频攻击与对抗技术的最新研究成果,并分析比较了各算法在不同条件下的优劣,同时还介绍了音频技术常用的数据集。最后结合该领域目前的研究现状,提出了说话人音频攻防对抗技术研究中亟待关注与研究的问题。

关键词：说话人音频音频伪造音频鉴伪音频数据集深度学习

立德树人任务下基于OBE理念“旅行社计调业务”课程思政实施路径

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河北旅游职业学院学报 2024年第1期29卷 77-81页

作者：李海梅孙志新河北旅游职业学院河北化承德067000

立德树人任务下基于OBE理念“旅行社计调业务”课程思政的设计思路:基于OBE理念要求,明确“旅行社计调业务”课程思政的目标,即要培养什么样的人;然后有针对性地设计并制定相关的课程思政结构以及教学内容,并在教学中采取多样化的教学方... 详细信息

立德树人任务下基于OBE理念“旅行社计调业务”课程思政的设计思路:基于OBE理念要求,明确“旅行社计调业务”课程思政的目标,即要培养什么样的人;然后有针对性地设计并制定相关的课程思政结构以及教学内容,并在教学中采取多样化的教学方法,以充分调动学生的积极性,引导学生主动思考和讨论;紧接着是根据OBE理念采取多元化的评价方式,重点评价学生的专业能力和思政素养,以进一步明确预先设计的课程思政模式是否存在改进之处,以为培养符合立德树人要求的高素质旅行社从业人才奠定基础。

关键词：立德树人 OBE思政旅行社计调业务

Secure P2P topology based on a multidimensional DHT space mapping

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Science China(Information Sciences) 2013年第5期56卷 224-237页

作者： sun zhixin LUO BingQing CHEN YaDang BU Kai Key Laboratory of Broadband Wireless Communication and Sensor Network Technology(Nanjing University of Posts and Telecommunications) Ministry of Education State Key Laboratory for Novel Software Technology Nanjing University

Distributed search and routing algorithms based on the DHT(distributed hash table) protocol have attracted considerable attention in structured P2P(peer-to-peer) research as a result of favorable properties such as distribution,self-organization,and high *** with a traditional C/S(client/server) network,the probability of peers initiating malicious behavior increases dramatically because of their self-governing and dynamic characteristics,which also make it harder to satisfy the peers’ security required by *** this paper,we propose a new secure DHT protocol based on a multidimensional mapping *** mechanism maps peers to a multidimensional space by dividing the identifiers into ***,a series of secure methods and routing algorithms are provided to achieve secure DHT in smaller *** with state-ofthe-art approaches,the theoretical analysis and experimental results show that the multidimensional mapping mechanism can effectively improve the average success rate of a resource search by inhibiting malicious behavior.

关键词： multidimensional mapping DHT(distributed hash table) structured P2P(peer-to-peer) network routing attack security

Research on Time Synchronization Method Under Arbitrary Network Delay in Wireless Sensor Networks

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Computers, Materials & Continua 2019年第9期61卷 1323-1344页

作者： Bing Hu Feng Xiang Fan Wu Jian Liu Zhe sun zhixin sun Key Laboratory of Broadband Wireless Communication and Sensor Network Technology Nanjing University of Posts and TelecommunicationsNanjing210003China YuanTong Express Co.LTD Shanghai201705China Department of Computer Science Tuskegee UniversityTuskegeeAL36088USA College of Information Engineering Nanjing University of Finance and EconomicsNanjing210023China

To cope with the arbitrariness of the network delays,a novel method,referred to as the composite particle filter approach based on variational Bayesian(VB-CPF),is proposed herein to estimate the clock skew and clock offset in wireless sensor ***-CPF is an improvement of the Gaussian mixture kalman particle filter(GMKPF)*** GMKPF,Expectation-Maximization(EM)algorithm needs to determine the number of mixture components in advance,and it is easy to generate overfitting and *** Bayesian EM(VB-EM)algorithm is introduced in this paper to determine the number of mixture components adaptively according to the ***,to solve the problem of data packet loss caused by unreliable links,we propose a robust time synchronization(RTS)method in this *** establishes an autoregressive model for clock skew,and calculates the clock parameters based on the established autoregressive model in case of packet *** final simulation results illustrate that VB-CPF yields much more accurate results relative to GMKPF when the network delays are modeled in terms of an asymmetric Gaussian ***,RTS shows good robustness to the continuous and random dropout of time messages.

关键词： Time synchronization particle filter expectation maximization wireless sensor networks(WSNs)

Numerical prediction of particle slip velocity in turbulence by CFD-DEM simulation

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Particuology 2023年第9期80卷 170-179页

作者： zhixin sun Xiaokang Yan Lijun Wang Xiaoheng Li Ai Wang Haijun Zhang School of Chemical Engineering China University of Mining and TechnologyXuzhou221116China School of Low-Carbon Energy and Power Engineering China University of Mining and TechnologyXuzhou221116China National Engineering Research Center of Coal Preparation and Purification China University of Mining and TechnologyXuzhou221116China School of Engineering The University of NewcastleCallaghanNSW2308Australia

Turbulent environment improves the flotation recovery of fine particles by promoting the particle–bubble collision rate,which directly depends on the particle slip ***,the existing slip velocity models are not applicable to fine particles in *** mechanism of turbulence characteristics and particle properties on the slip velocity of fine particles in turbulence was *** this study,a coupled ANSYS FLUENT and EDEM based on computational fluid dynamics(CFD)and discrete element method(DEM)were used to simulate the slip velocity of fine particles in the approximately homogenous isotropic turbulence,which was excited by the *** reliability of the used CFD-DEM simulation method was validated against the slip velocity measured by the particle image velocimetry(PIV)*** particular,the effects of the particle shapes,particle densities,and turbulence intensities on the slip velocity have been investigated with this numerical *** results show that particle shapes have no significant effect on fine particles between 37 and 225μ*** slip velocity of the spherical particles increases with the turbulence intensity and particle *** on the simulated data,a model which has a correlation coefficient of 0.95 is built by using nonlinear fitting.

关键词： Slip velocity Fine particles Turbulence CFD-DEM

Convergence analysis and application of the central force optimization algorithm

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Science China(Information Sciences) 2015年第5期58卷 208-210页

作者： MENG Chao GONG Jing LIU SanMin sun zhixin Key Laboratory of Broadband Wireless Communication and Sensor Network Technology Nanjing University of Posts and TelecommunicationsMinistry of Education College of Mathematics & Physics Nanjing University of Posts and Telecommunications College of Computer and Information Anhui Polytechnic University

Dear editor,The Central Force Optimization(CFO)[1]is a new heuristic *** contrast with the stochastic algorithms such as Particle Swarm Optimization(PSO)[2],Ant Colony Optimization[3]and other such algorithms[4–6],CF... 详细信息

关键词： CFO Convergence analysis and application of the central force optimization algorithm

Designing and Optimization of Fuzzy Sliding Mode Controller for Nonlinear Systems

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Computers, Materials & Continua 2019年第7期61卷 119-128页

作者： Zhe sun Yunrui Bi Songle Chen Bing Hu Feng Xiang Yawen Ling zhixin sun Nanjing University of Posts and Telecommunications Nanjing210003China Nanjing Institute of Technology Nanjing211167China YuanTong Express Co.LTD. Shanghai201705China

For enhancing the control effectiveness,we firstly design a fuzzy logic based sliding mode controller(FSMC)for nonlinear crane *** basis of overhead crane dynamic characteristic,the sliding mode function with regard to trolley position and payload ***,in order to eliminate the chattering problem of sliding mode control,the fuzzy logic theory is adopted to soften the control ***,aiming at the FSMC parameter setting problem,a DE algorithm based optimization scheme is proposed for enhancing the control ***,by implementing the computer simulation,the DE based FSMC can effectively tackle the overhead crane sway problem and avoid unexpected accident greatly.

关键词： Sliding mode control fuzzy logic theory systems optimization

A NEW DYNAMIC DEFENSE MODEL BASED ON ACTIVE DECEPTION

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Journal of Electronics(China) 2009年第2期26卷 205-213页

作者： Gong Jing sun zhixin Gu Qiang College of Mathematics ＆ Physics Nanjing University of Posts and Telecommunications Nanjing 210003 China Institute of Computer Nanjing University of Posts and Telecommunications Nanjing 210003 China State Key Laboratory for Novel Software Technology Nanjing University Nanjing 210093 China Jiangsu Mobile Communication Company Limited Nanjing 210029 China

Aiming at the traditional passive deception models,this paper constructs a Decoy Platform based on Intelligent Agent(DPIA) to realize dynamic *** paper explores a new dynamic defense model based on active deception,introduces its architecture,and expatiates on communication methods and security guarantee in information *** results show that the DPIA can attract hacker agility and activity,lead abnormal traffic into it,distribute a large number of attack data,and ensure real network security.

关键词： Network security Active deception Dynamic defense