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Synthesis of AgInS_2 QDs in droplet microreactors:Online fluorescence regulating through temperature control

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Chinese Chemical Letters 2019年第1期30卷 79-82页

作者： Haotian Ma Liangjun Pan Ji Wang Li zhang zhiling zhang Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) College of Chemistry and Molecular Sciences Wuhan University

For the synthesis of AgInS_2 quantum dots(QDs), a suitable temperature is extremely important for control of the size, shape and fluorescence properties of QDs. Most of synthesis methods for AgInS_2 QDs are based on batch reactors, which bring uneven distribution of temperature, affecting their fluorescence properties and size uniformity. Here we designed a droplet microreactor with a temperature-controllable region, and successfully synthesized water-soluble AgInS_2 QDs. By accurately controlling temperature,we also studied how the reaction temperature affected the fluorescence properties of AgInS_2 QDs. The results showed that with the increasing of reaction temperature, the QDs size increased and the fluorescence peak constantly red-shifted along with enhanced fluorescence intensity. Based on the droplet microreactor, we could achieve more appropriate reaction condition to synthesize AgInS_2 QDs with higher fluorescence quantum yield(QY) and intensity.

关键词： AgInS2 Quantumdot Droplet microreactor Temperature Fluorescence property

Construction of CdSe/ZnS quantum dot microarray in a microfluidic chip

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Science China Chemistry 2012年第4期55卷 543-549页

作者： LIU HongWei JING YingYing YU Xu PANG DaiWen zhang zhiling Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education) College of Chemistry and Molecular SciencesWuhan UniversityWuhan 430072China Institute of Biomedical and Health Engineering Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity and stability of the signal. Recently, several nanomaterials, especially semiconductor nanomaterials, due to their excellent fluorescence properties, have been widely used to construct microarrays for biosensors. Here, we presented an approach for constructing CdSe/ZnS quantum dot (QD) microarray in microfluidic channels on a glass slide by photolithography. The conditions for immobilizing stable and uniform QD microarray on the glass slide were optimized. Several types of QD microarrays with different emission wavelengths and modified groups were constructed using silanization and lithography technology. Based on the fluorescence quenching effect of Cu2+ on QDs, the microfluidic chip with QD microarray was applied for the determination of Cu2+. 1 nmol/L Cu2+ could be detected by this method.

关键词： microarray quantum dots microfluidic Cu2+

Controllable and flexible cellular network for virus cell-to-cell spread

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Chinese Chemical Letters 2019年第6期30卷 1229-1232页

作者： Qiong Yan Cheng Wang Ji Wang Liangjun Pan zhiling zhang Key Laboratory of Analytical Chemistry for Biology(Ministry of Education) College of Chemistry and Molecular Sciences Wuhan University

Virus spread is closely related to *** research methods of virus spread do not distinguish cell-to-cell spread from cell-free *** uncertainty of virus induced plaque and virus spread direction makes it difficult to track the spread of virus in ***,the cellular network was fabricated on the basis of PEG modification and soft ***,cell numbers and spatial distributions we re p recisely controlled,e.g.,cellular netwo rks like "WHU" can be ***,a micro-injector was combined with the cellular network for virus spread on the fixed point,in which virus spread direction was limited in one *** results suggested that controllable and flexible cellular network can be constructed on the PEG *** synergia of micro-injector and cellular network provides an advanced tool to investigate virus cell-to-cell spread at the initial infection stage.

关键词： Cellular network Cell-to-cell spread Micro-injector Pseudorabies virus PEG pattern

Topography guiding the accelerated and persistently directional cell migration induced by vaccinia virus

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Chinese Chemical Letters 2020年第1期31卷 167-171页

作者： Cheng Wang Ji Wang Dandan Fu Qiong Yan Daiwen Pang zhiling zhang Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) College of Chemistry and Molecular Sciences State Key Laboratory of Virology Wuhan University

The rapid transmission of vaccinia virus(VACV)in vivo is thought to be closely related to the cell migration induced by *** migration involved in dynamic changes of cell-substrate adhesion and actin cytoskeleton organization,which can influence by the micro/nano-scale topographic structures that cells are naturally exposed to via contact ***,migration behaviors of VACV-infected cells exposed to topographic cues are still ***,we designed an open chip with microgrooved poly(dimethyl siloxane)(PDMS)substrate to explore the topography roles in VACV-induced cell *** from the random cell migration observed in traditional scratch assay on planar substrate,VACV-infected cells had a tendency to persistently migrate along the axis parallel to microgroove with increased ***,infected cells exhibited a dominant elongated protrusion aligned to the micro-grating axis compare to the shorter lamella extended in any direction on smooth ***,the Golgi complex preferred to relocate behind the nucleus confined within the micro-grating axis in majority of infected migratory *** directional polarization of cells embodied in protrusion formation and Golgi reorientation was responsible for the directionally persistent migration behaviors induced by VACV on microgrooved *** cells response to substrate topography,causing the actin-filled stretched protrusion containing numerous virions and accelerated movement is likely to facilitate direct and rapid spread of *** work opens a window for us to understand the migration behaviors of infected cells in vivo,and also provides a cue for revealing the relationship between virus-induced cell migration and virus rapid spread.

关键词： Cell migration Topography Vaccinia virus Cytoskeleton Contact guidance Micro-patterned substrate

Measuring radial Young’s modulus of DNA by tapping mode AFM

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Chinese Science Bulletin 2007年第23期52卷 3189-3192页

作者： LIN Yi SHEN XinCheng WANG JingJing BAO Lei zhang zhiling PANG DaiWen College of Chemistry and Molecular Sciences State Key Laboratory of Virology Wuhan University Wuhan 430072 China

Based on tapping mode AFM imaging, a method was demonstrated to evaluate compression elasticity of single double-stranded DNA (dsDNA) molecules in the force region. With images under ambient conditions, Young’s moduli of dsDNA in compression were calculated. Results demonstrated that Young’s moduli of dsDNA can be simply deduced according to the proposed model. The method can also be used to evaluate the compression elasticity of similar soft nanomaterials.

关键词：测量光线抽头模型弹性显微镜

Precisemodification of the surface charge of antigen enhances vaccine immunogenicity

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The Innovation 2023年第4期4卷 83-90页

作者： Xiaodong Zai zhiling zhang Chuge Zhou Fangxin Zhao Yue zhang Xiaolin Wang Ruihua Li Yaohui Li Xiaofan Zhao Shuyi Wang Yilong Yang Ying Yin Jun zhang Junjie Xu Wei Chen Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China College of pharmacy Nanjing University of Chinese MedicineNanjing 210023China School of Medicine Zhejiang UniversityHangzhou 310058China

Aluminum(alum)adjuvant is the most extensively used protein subunit vaccine adjuvant,and its effectiveness and safety have been widely *** surface charge of the antigen determines its electrostatic adsorption to alum adjuvant,which directly affects the immune efficacy of the protein *** our study,we precisely modified its surface charge by inserting charged amino acids into the flexible region of the SARS-CoV-2 receptorbinding domain(RBD),achieving electrostatic adsorption and a site-specific anchor between the immunogen and alum adjuvant.

关键词： charge electrostatic adsorption

Research and Design of Dead Message Handling Mechanism in JMS

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通讯和计算机（中英文版） 2010年第6期7卷 14-18页

作者： Lili Wang Shuang Wei zhiling zhang School of Software Chongqing Information Technology College Chongqing Wanzhou 404000 China

基于侧向磁泳的微流控芯片对两种磁性纳米球的同时分选

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高等学校化学学报 2020年第6期41卷 1243-1251页

作者：张作然张利张志凌武汉大学化学与分子科学学院武汉430072

基于磁性纳米球在微流控芯片上的侧向磁泳,利用微流控芯片分选了不同磁响应性的磁球.提出了包含磁性纳米球聚集与偏移的理论模型,用于分析磁球在芯片上的侧向位移.在理论分析的基础上设计了芯片系统,使不同磁响应性的磁纳米球可以在芯... 详细信息

基于磁性纳米球在微流控芯片上的侧向磁泳,利用微流控芯片分选了不同磁响应性的磁球.提出了包含磁性纳米球聚集与偏移的理论模型,用于分析磁球在芯片上的侧向位移.在理论分析的基础上设计了芯片系统,使不同磁响应性的磁纳米球可以在芯片系统上依次被分选.实验结果表明,2种磁性纳米球的分选效率均可接受,且实验操作简单;磁响应性强的磁球可被完全分离,这对于珍贵分析样品的分选很有价值.该分选系统被成功用于同时分选样品中乙型肝炎病毒的DNA与丙型肝炎病毒的反转录DNA,在生化分析中具有广阔的应用前景.

关键词：微流控芯片磁性纳米球磁分选

An improved ileal conduit surgery for bladder cancer with fewer complications

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Cancer Communications 2019年第1期39卷 178-187页

作者： Zhiyong Li Zhuowei Liu Kai Yao Zike Qin Hui Han Yonghong Li Pei Dong Yunlin Ye Yanjun Wang Zhiming Wu zhiling zhang Fangjian Zhou Department of Urology State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterNo.651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China

Background:Radical cystectomy and urinary diversion remains the standard surgical treatment for patients with muscle-invasive or high-risk or recurrent non-muscle-invasive bladder *** this approach prolongs patient survival remarkably,there are postoperative complications associated with urinary *** study aimed to assess the efficacy of modified ileal conduit surgery for reducing early and late stoma-and ureteroileal anastomo-sis-related complications,as compared with conventional ileal conduit urinary ***:We retrospectively evaluated the clinical data of bladder cancer patients treated with radical cystectomy and ileal conduit urinary diversion at Sun Yat-sen University Cancer Center between January 1,2000 and June 30,*** conduit was created by the conventional or a modified *** clinicopathologic features of the conventional and the modified ileal conduit groups were compared using the t test and the Chi square ***-able logistic regression analysis and multivariable Cox regression analysis were performed to determine the odds of developing stoma-and ureteroileal anastomosis-related complications in the two ***:145 and 100 patients underwent the modified and conventional ileal conduit surgery,*** two groups were comparable with regard to clinicopathologic *** rate of stoma-related complications was significantly lower in the modified ileal conduit group than in the conventional ileal conduit group(0.7%vs.17.0%,P<0.001).No late stoma-related complications were seen in the modified ileal conduit group,but were seen in 13(13.0%)patients in the conventional ileal conduit *** rate of ureteroileal anastomosis-related complications was significantly lower in the modified ileal conduit group than in the conventional ileal conduit group(4.8%vs.15.0%,P=0.001).In multivariable analyses,the modified ileal conduit group was significantly less likely to develop stoma-(odds rati

关键词： Bladder cancer Cystectomy Urinary diversion Ileal conduit Complication Surgical technique