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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw zhijun tong Yirong Yang Zhiwei Wang Liying Zhang Lihua Deng Zhiguo Zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

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Population amount risk assessment of extreme precipitation-induced landslides based on integrated machine learning model and scenario simulation

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Geoscience Frontiers 2023年第3期14卷 163-179页

作者： Guangzhi Rong Kaiwei Li zhijun tong Xingpeng Liu Jiquan Zhang Yichen Zhang Tiantao Li School of Environment Northeast Normal UniversityChangchun 130024China Key Laboratory for Vegetation Ecology Ministry of EducationChangchun 130117China State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration Northeast Normal UniversityChangchun 130117China School of Emergency Management Changchun Institute of TechnologyChangchun 130012China Chengdu University of Technology Chengdu 610059China State Key Laboratory of Geohazard Prevention and Geo Environment Protection Chengdu University of TechnologyChengdu 610059China

In this study,the future landslide population amount risk(LPAR)is assessed based on integrated machine learning models(MLMs)and scenario simulation techniques in Shuicheng County,***,multiple MLMs were selected and hyperparameters were optimized,and the generated 11 models were crossintegrated to select the best model to calculate landslide susceptibility;by calculating precipitation for different extreme precipitation recurrence periods and combining the susceptibility results to assess the landslide *** the town as the basic unit,the exposure and vulnerability of the future landslide population under different Shared Socioeconomic Pathways(SSPs)scenarios in each town were assessed,and then combined with the hazard to estimate the LPAR in *** results showed that the integrated model with the optimized random forest model as the combination strategy had the best comprehensive performance in susceptibility *** distribution of hazard classes is similar to susceptibility,and with an increase in precipitation,the low-hazard area and high-hazard decrease and shift to medium-hazard and very high-hazard *** high-risk areas for future landslide populations in Shuicheng County are mainly concentrated in the three southwestern towns with high vulnerability,whereas the northern towns of Baohua and Qinglin are at the lowest risk *** LPAR increased with the intensity of extreme *** LPAR differs significantly among the SSPs scenarios,with the lowest in the“fossil-fueled development(SSP5)”scenario and the highest in the“regional rivalry(SSP3)”*** summary,the landslide susceptibility model based on integrated machine learning proposed in this study has a high predictive *** results of future LPAR assessment can provide theoretical guidance for relevant departments to cope with future socioeconomic development challenges and make corresponding disaster prevention and mitigation plans to prevent landsli

关键词： Landslide population amount risk assessment Integrated Machine Learning Extreme precipitation scenarios Future socioeconomic development scenarios

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Quantifying Multi-hazards and Impacts Over Different Growth Periods of Maize:A Study Based on Index Construction

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International Journal of Disaster Risk Science 2023年第5期14卷 822-839页

作者： Dan Chen Ying Guo Rui Wang Yunmeng Zhao Kaiwei Li Jiquan Zhang Xingpeng Liu zhijun tong Chunli Zhao School of Environment Northeast Normal UniversityChangchun130024China Jilin Province Science and Technology Innovation Center of Agro-Meteorological Disaster Risk Assessment and Prevention Northeast Normal UniversityChangchun130024China Key Laboratory for Vegetation Ecology Ministry of EducationChangchun130024China State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration Northeast Normal UniversityChangchun130024China College of Forestry and Grassland Jilin Agricultural UniversityChangchun130024China

Owing to the complexity and variability of global climate,the study of extreme events to ensure food security is particularly *** standardized precipitation requirement index(SPRI)and chilling injury index(I_(Ci))were introduced using data from agrometeorological stations on the Songliao Plain between 1981 and 2020 to identify the spatial and temporal variability of drought,waterlogging,and low-temperature cold damage during various maize growth *** drought and low-temperature cold damage events(CDLEs)and compound waterlogging and low-temperature cold damage events(CWLEs)were then *** measure the intensity of compound events,the compound drought and low-temperature cold damage magnitude index(CDLMI),and compound waterlogging and low-temperature cold damage magnitude index(CWLMI)were constructed by fitting marginal ***,the effects of extreme events of various intensities on maize output were *** findings demonstrate that:(1)There were significant differences in the temporal trends of the SPRI and ICiduring different maize growth *** predominated in the middle growth period(MP),waterlogging predominated in the early growth period(EP)and late growth period(LP),and both drought and waterlogging tended to increase in intensity and *** frequency of low-temperature cold damage showed a decreasing trend in all periods.(2)The CDLMI and CWLMI can effectively determine the intensity of CDLEs and CWLEs in the study area;these CDLEs and CWLEs had higher intensity and frequency in the late growth period.(3)Compared to single events,maize relative meteorological yield had a more significant negative correlation with the CDLMI and CWLMI.

关键词： Compound events magnitude index Drought Growth periods Low-temperature cold damage Spatiotemporal distribution Waterlogging

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Surface tension of single suspended aerosol microdroplets

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Chinese Chemical Letters 2024年第4期35卷 520-524页

作者： Yukai tong zhijun Wu Bo Zhou Min Hu Anpei Ye Key Laboratory for the Physics and Chemistry of Nanodevices School of ElectronicsPeking UniversityBeijing 100871China State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China Aerospace Information Research Institute Chinese Academy of SciencesBeijing 100094China

The surface tension of troposphere aerosols can significantly influence their atmospheric processes and key properties, particularly on the morphology, the phase transition, the activation as cloud condensation nuclei, and the gas-particle partitioning. However, directly measuring the surface tension of single ambient aerosol is quite challenging, due to the limitations of their picolitre volumes and thermal motion. Here, we developed a dual laser tweezers Raman spectroscopy(DLT-RS) system to directly sense the surface tension of single airborne microdroplets(PM10particles). A pair of aerosol droplets were trapped and driven to coalesce by the laser tweezers. Meanwhile, the backscattering light intensity and brightfield images during the coalescence process were recorded to characterize the aerosol surface tension. A remarkable advantage of directly sensing aerosol surface tension is that the solutes in aerosols are often supersaturated, which is common in atmospheric aerosols but almost unavailable in bulk *** experimentally measured the surface tension of aerosols composed of nitrates or oxalic acid/nitrate mixture. Besides, the variation of surface tension during aerosol aging process was also explored, which brings possible implications on the surface evolution of actual ambient aerosol during their atmospheric lifetime.

关键词： Surface tension Droplet coalescence Single aerosol Laser tweezers Aerosol aging

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Precise mode control of laser-written waveguides for broadband,low-dispersion 3D integrated optics

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Light(Science & Applications) 2024年第6期13卷 1186-1195页

作者： Yuying Wang Lijing Zhong Kuen Yao Lau Xuhu Han Yi Yang Jiacheng Hu Sergei Firstov Zhi Chen zhijun Ma Limin tong Kin Seng Chiang Dezhi Tan Jianrong Qiu College of Optical Science and Engineering Zhejiang University310027HangzhouChina Institute of Light+X Science and Technology College of Information Science and EngineeringNingbo University315211NingboChina School of Optoelectronic Science and Engineering&Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow University215006SuzhouChina Prokhorov General Physics Institute of the Russian Academy of Sciences Dianov Fiber Optics Research Center38 Vavilov str.Moscow119333Russia Zhejiang Lab 311121HangzhouChina College of Materials Science and Engineering Key Laboratory of Advanced Materials of Yunnan ProvinceKunming University of Science and Technology650093KunmingYunnanChina Department of Electrical Engineering City University of Hong Kong83 Tat Chee AvenueKowloonHong Kong SARChina School of Materials Science and Engineering Zhejiang University310027HangzhouChina

Three-dimensional(3D)glass chips are promising waveguide platforms for building hybrid 3D photonic circuits due to their 3D topological capabilities,large transparent windows,and low coupling *** present,the key challenge in scaling down a benchtop optical system to a glass chip is the lack of precise methods for controlling the mode field and optical coupling of 3D waveguide ***,we propose an overlap-controlled multi-scan(OCMS)method based on laser-direct lithography that allows customizing the refractive index profile of 3D waveguides with high spatial precision in a variety of *** the basis of this method,we achieve variable mode-field distribution,robust and broadband coupling,and thereby demonstrate dispersionless LP21-mode conversion of supercontinuum pulses with the largest deviation of<0.1 dB in coupling ratios on 210 nm *** approach provides a route to achieve ultra-broadband and low-dispersion coupling in 3D photonic circuits,with overwhelming advantages over conventional planar waveguide-optic platforms for on-chip transmission and manipulation of ultrashort laser pulses and broadband supercontinuum.

关键词： waveguide mode dispersion

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Fine mapping of cisc(t),a gene for cold-induced seedling chlorosis,and identification of its candidate in rice

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Chinese Science Bulletin 2010年第27期55卷 3149-3153页

作者： LAN Tao WANG Bin LING QiuPing XU ChunHua tong zhijun LIANG KangJing DUAN YuanLin JIN Jing WU WeiRen Key Laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of Crops Fuzhou 350002 China Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding Fuzhou 350002 China

The seedlings of indica rice cultivar Dular are susceptible to chlorosis under low temperature *** previous studies indicated that low temperature-induced seedling chlorosis is controlled by a recessive gene,located between SSR markers RM257 and RM242,on the long arm of chromosome *** temporarily named the gene cisc(t).Using a large F2 population derived from a cross between Dular and the japonica cultivar Lemont,which displays a normal green color at low temperatures,cisc(t)was fine mapped to within a 12-kb *** is only one annotated gene in this interval,which encodes a pentatricopeptide repeat(PPR)*** analysis indicated that 8 bases were deleted at the 60th base in the Dular allele,resulting in a frame-shift mutation and loss of function of the *** is consistent with the chlorosis mutant phenotype of *** addition,previous studies have shown that many chlorosis mutants of seedlings are related to PPR ***,we presume that the PPR gene is the candidate for cisc(t).

关键词：黄化现象隐性基因 CISC 精细定位幼苗鉴定水稻低温条件

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Wetting sub-nanochannels via ionic hydration effect for improving charging dynamics

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Green Energy & Environment 2024年第3期9卷 473-480页

作者： Yayun Shi Xiaoli Zhao Qihang Liu Zhenghui Pan Congcong Liu Shanyi Zhu zhijun Zuo Xiaowei Yang State Key Laboratory of Clean and Efficient Coal Utilization Taiyuan University of TechnologyTaiyuan030024China School of Materials Science and Engineering Tongji UniversityShanghai201804China School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai200240China

The ionic transport in sub-nanochannels plays a key role in energy storage,yet suffers from a high energy *** sub-nanochannels is crucial to accelerate ionic transport,but the introduction of water is challenging because of the hydrophobic extreme *** propose wetting the channels by the exothermic hydration process of pre-intercalated ions,the effect of which varies distinctly with different ionic hydration structures and *** to the failed pre-intercalation of SO_(4)^(2-),HSO_(4)^(-) with weak hydration energy results in a marginal effect on the HOMO(Highest Occupied Molecular Orbital)level of water to avoid water splitting during the electrochemical ***,the ability of water introduction is reserved by the initial incomplete dissociation state of HSO_(4)^(-),so the consequent exothermic reionization and hydration processes of the intercalated HSO_(4)^(-) promote the water introduction into sub-nanochannels,finally forming the stable confined water through hydrogen bonding with functional *** wetted channels exhibit a significantly enhanced ionic diffusion coef-ficient by~9.4 times.

关键词： Sub-nanochannels Ionic hydration Ionic transport Supercapacitors Confined water

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Development of DArT markers for a linkage map of flue-cured tobacco

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Chinese Science Bulletin 2013年第6期58卷 641-648页

作者： LU XiuPing GUI YiJie XIAO BingGuang LI YongPing tong zhijun LIU Yun BAI XueFei WU WeiRen XIA Ling HUTTNER Eric KILIAN Adrzej FAN LongJiang Department of Agronomy Zhejiang University Yunnan Academy of Tobacco Agricultural Sciences and China Tobacco Breeding Research Center at Yunnan DArT P/L PO Box 7141YarralumlaCanberraACT 2600Australia

Tobacco(Nicotiana tabacum) is one of the most economically important nonfood crops,and flue-cured tobacco accounts for approximately 80% of world tobacco *** extremely narrow genetic diversity in the tobacco pool has led to a low efficiency of PCR-based molecular markers(such as AFLP and SSR).Diversity Arrays Technology(DArT) is a high-throughput hybridisation-based marker system that has been developed in many plants including wheat,which,like tobacco,has a complex *** this study,we developed a tobacco DArT chip that included 7680 representative sequence tags based on typical tobacco *** 1076 DArT markers of flue-cured tobacco were identified and most(82.1%) of their polymorphism information contents(PICs) were greater than *** integrated linkage map that included 851 markers(238 DArT and 613 SSR),which is the highest density map of flue-cured tobacco to date,was *** chip-based DArT system provides an alternative in high-throughput marker genotyping for tobacco.

关键词： DART 分子标记连锁图谱烤烟烟草生产遗传多样性多态信息含量标记系统

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基于适宜性和空间功能值的德兴市矿粮复合区“三生”空间优化研究

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水土保持研究 2024年第1期31卷 395-407页

作者：邓彤罗志军曾建玲江西农业大学国土资源与环境学院南昌330045

[目的]深入开展“三生”功能评价与空间调控优化,协调重构不合理的空间布局与结构,缓解矿粮空间冲突,为国土空间治理和区域高质量发展提供参考。[方法]以德兴市矿粮复合区为例,结合空间适宜性评价、功能值测算与空间自相关分析,基于AVC... 详细信息

[目的]深入开展“三生”功能评价与空间调控优化,协调重构不合理的空间布局与结构,缓解矿粮空间冲突,为国土空间治理和区域高质量发展提供参考。[方法]以德兴市矿粮复合区为例,结合空间适宜性评价、功能值测算与空间自相关分析,基于AVC“三力”理论对研究区进行“三生”空间优化研究。[结果](1)农业、工业生产空间分别集中于西南部和北部,生活空间集中度自西北向东南递减,而生态空间分布广泛且整体均衡;(2)生产、生态空间多呈现聚集态势,而生活空间功能聚集性较弱,呈零星分布,且矿区对其空间适宜性、功能值及聚集度有负面影响;(3)空间重构后,生活、生态空间功能值分别从1.948 6亿元、90.755 0亿元提升至1.951 8亿元、90.778 9亿元。[结论]综合考虑适宜性、空间功能值与集中度进行空间优化与重构,研究区生活与生态空间功能值有所提高,各类空间分布趋于集聚态势,缓解了矿区对于人们生活与粮食生产的负面影响。未来应针对各类空间进行系统治理与管控,并严格控制矿区开发,以保障生产、生活与生态功能。

关键词： “三生”空间适宜性空间自相关空间优化矿粮复合区

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益母草颗粒HPLC指纹图谱及4个指标成分含量研究

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中国药房 2022年第5期33卷 586-591页

作者：朱子聪边雨高陆宋德库佟志军长春中医药大学药学院长春130117 吉林省现代中药工程研究中心有限公司长春130033 吉林天力泰药业有限公司吉林白山134400

目的建立益母草颗粒的高效液相色谱(HPLC)指纹图谱,并对其中盐酸益母草碱、阿魏酸、芦丁、金丝桃苷4个指标成分进行含量测定。方法采用Inertsil^(TM)ODS-3色谱柱,以乙腈(A)-0.1%甲酸溶液(B)为流动相进行梯度洗脱,流速为1.0 mL/min,检测... 详细信息

目的建立益母草颗粒的高效液相色谱(HPLC)指纹图谱,并对其中盐酸益母草碱、阿魏酸、芦丁、金丝桃苷4个指标成分进行含量测定。方法采用Inertsil^(TM)ODS-3色谱柱,以乙腈(A)-0.1%甲酸溶液(B)为流动相进行梯度洗脱,流速为1.0 mL/min,检测波长为280 nm,柱温为25℃,进样量为5μL。采用《中药色谱指纹图谱相似度评价系统(2012版)》建立10批益母草颗粒的HPLC指纹图谱并分析其相似度,通过与对照品图谱比对进行共有峰指认。使用SPSS 25.0软件和SIMCA 13.0软件进行聚类分析和主成分分析;同时,采用HPLC法测定益母草颗粒中盐酸益母草碱、阿魏酸、芦丁、金丝桃苷4个指标成分的含量。结果建立了10批益母草颗粒的HPLC指纹图谱,共匹配出16个共有峰,并指认出6号峰为盐酸益母草碱、13号峰为阿魏酸、14号峰为芦丁、16号峰为金丝桃苷,10批样品的相似度均大于0.970。通过聚类分析和主成分分析,10批样品均被分为4类,分类结果一致。样品中盐酸益母草碱、阿魏酸、芦丁、金丝桃苷的含量分别为122.10~138.82、9.33~10.45、14.12~18.95、5.87~8.06μg/g。结论建立的益母草颗粒HPLC指纹图谱及4个指标成分含量测定方法简便易行、准确度高、重复性好,可为益母草颗粒的质量评价提供参考。

关键词：益母草颗粒指纹图谱聚类分析主成分分析含量测定盐酸益母草碱阿魏酸芦丁金丝桃苷

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