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MEMOIR: A Novel System for Neural Lineage Tracing

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Neuroscience Bulletin 2017年第6期33卷 763-765页

作者： zhifu wang Jianhong Zhu Department of Neurosurgery Fudan University Huashan Hospital National Key Laboratory of Medical Neurobiology The Institutes of Brain Science and The Collaborative Innovation Center for Brain Science Shanghai Medical College Fudan University Translational Medical Center for Stem Cell Therapy Shanghai East Hospital

Memory by Engineered Mutagenesis with Optical In situ Readout（MEMOIR）is a novel strategy for lineage tracing that combines Cas9/g RNA and sequential multiplexed single-molecule RNA fluorescence hybridization（seqFISH）[1],which was created by Cai Long et *** the California Institute of Technology[2].In MEMOIR,dynamic cellular event histories are recorded,then read out in single cells using seq ***,we introduce the

关键词： MEMOIR A Novel System for Neural Lineage Tracing

Track Tension Analysis of Four-Wheel Drive Tracked Vehicles

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Journal of Beijing Institute of Technology 2017年第1期26卷 45-49页

作者： zhifu wang Bin Liu Li Zhai Collaborative Innovation Center of Electric Vehicles in Beijing Beijing 100081 China School of Mechanical Engineering Beijing Institute of Technology Beijing 100081 China

The distribution of track tension on track link is complex when the tracked vehicles run at a high speed.A multi-drive track link structure,which changes the traditional induction wheel into the driving wheel was *** mathematical model of the system was established and the distribution of track tension was *** combined simulation model of RecurDyn and Simulink of the structure with multi-drive track was *** simulation results show that our proposed structure has more uniform tension distribution than traditional structures,especially under the high speed *** maximum tension can be reduced by 28 kN-36 kN and the transmission efficiency can be improved by10%-16% under high speed condition with this new structure.

关键词： tracked vehicle four-wheel drive track tension

Thermal analysis of surface-mounted permanent magnet synchronous motor for electric vehicle application

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High Technology Letters 2010年第1期16卷 80-84页

作者：范金鑫 Zhang Chengning wang zhifu School of Mechanical and Vehicle Engineering Beijing Institute of Technology Beijing 100081 P.R. China

As a driving motor, surface mounted permanent magnet synchronous motor exhibits high efficiency and high power density. However, it is susceptible to suffer irreversible demagnetization and insulation failure of coils under severe thermal load condition. Therefore, it is essential to predict temperattrre distribution in the driving motor. In this paper, a lumped parameter thermal mode/of surface mounted permanent magnet is investigated. By using finite element method, the iron loss distribution in various parts of the driving motor is achieved. Moreover, the influences of interface gap and flow rate on temperature distribution are discussed. Finally, the simulation of temperature distribution in different parts of the driving motor is achieved. The presented methodology contributes to verify the feasibility of the driving motor design.

关键词： thermal analysis lumped-parameter model heat transfer permanent magnet synchronous motor (PMSM) electric vehicle (EV)

Effects of composite scale on high temperature oxidation resistance of Fe-Cr-Ni heat resistant alloy

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China Foundry 2009年第2期6卷 109-114页

作者： wang Haitao wang Yuqing Yu Huashun Min Guanghui wang zhifu Key Laboratory for Liquid Structure and Heredity of Materials of Education Ministry School of Materials Science and Engineering Shandong University Jinan 250061 China Jinan Railway Institute of Technology Jinan 250013 China

Fe-Cr-Ni heat resistant alloys with aluminum and silicon addition, alone and in combination, were melted using an intermediate frequency induction furnace with a non-oxidation method. By the oxidation weight gain method, the oxidation resistances of the test alloys were determined at 1,200 ℃ for 500 hours. According to the oxidation weight gains, the oxidation kinetic curves were plotted and the functions were regressed by the least squares method. The results show that the oxidation kinetic curves follow the power function of y = ax^b （a〉0, 0〈b〈1）. The effects of scale compositions on oxidation resistance were studied further by analyses using X-ray diffraction （XRD） and scanning electron microscope （SEM）. It is found that the composite scale compounds of Cr203, a-Al2O3, SiO2 and FeCr2O4, with compact structure and tiny grains, shows complete oxidation resistance at 1,200℃. When the composite scale lacks a-Al2O3 or SiO2, it becomes weak in oxidation resistance with a loose structure. By the criterion of standard Gibbs formation free energy, the model of the nucleation and growth of the composite scale is established. The forming of the composite scale is the result of the competition of being oxidized and reduced between aluminum, silicon and the matrix metal elements of iron, chromium and nickel. The protection of the composite scale is analyzed essentially by electrical conductivity and strength properties.

关键词： Fe-based alloy heat resistant alloy oxide scale oxidation resistance

Study on load performance of electric motor system used in hybrid electric vehicle

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High Technology Letters 2010年第1期16卷 63-66页

作者：李雯 Zhang Chengning wang zhifu Gao Lei National Engineering Laboratory for Electric Vehicle Beijing Institute of Technology Beijing 100081 P.R. China New Energy Vehicle Division SAIC Motor Corporation Limited Shanghai 201804 P.R. China

The performance of the power assist, global optimization solved by dynamic programming （DP） method, Chery and Insight control strategies are analyzed using the mild parallel hybrid electric vehicle （PHEV） model based on Insight structure. The influence of the four control strategies to the load power of the electric motor system used on parallel hybrid electric vehicle is studied. It is found that 80 percent of the motor load power points are under 1/5 of the electric peak power. The motor load power of the power assist control strategy is distributed in the widest range during generating operation, and the motor load power of the global optimization control strategy has the smallest one.

关键词： parallel hybrid electric vehicle (PHEV) management strategy motor load power

Evaluation of the Bioavailability of Metals in Sediment from the Southern Coastal Wetland of the Qiantang Estuary by Using Diffusive Gradients in Thin Films Technique

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Journal of Ocean University of China 2022年第2期21卷 375-387页

作者： FENG Weihua wang zhifu ZHU Wenzhuo ZHENG Fangqin ZHANG Dongrong XU Hengtao Key Laboratory of Engineering Oceanography Second Institute of OceanographyMNRHangzhou 310012China College of Marine Science&Technology Zhejiang Ocean UniversityZhoushan 316022China

Metal pollution has become an major issue governing the wetland ecosystem *** southern coastal wetland of the Qiangtang Estuary are facing unusual perturbation due to rapid development along the embayment in recent *** study evaluated the bioavailability of metals(Cu,Pb,Cd,Cr and Zn)in the sediment of the southern coastal wetland of the Qiangtang Es-tuary using diffusive gradients in thin films(DGT)techniques and compared with several methods based on total metal *** results showed that the contents of Cr,Pb,Cd and Cu in sediment,as detected using DGT,were considerably correlated with the exchangeable fraction and the content in Phragmites australis roots,while a weak correlation was observed for ***,DGT analysis could be used to evaluate the bioavailability and potential risk of Cr,Cd,Pb and Cu for *** indices,such as DGT concentration,bioaccumulation in ***,geoaccumulation index(Igeo)and potential ecological risk index(RI),revealed that Cd was a major potential ecological risk factor along the southern coast wetland of the Qiantang Estuary,especially in the upstream region,which is potentially more vulnerable to the anthropogenic pollution.

关键词： diffusive gradients in thin films estuarine wetland metal bioavailability

Effects of ammonium and nitrate on encystment and growth of Scrippsiella trochoidea

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Chinese Science Bulletin 2014年第33期59卷 4491-4497页

作者： zhifu wang Zhiming Yu Xiuxian Song Xihua Cao Yue Zhang Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences University of Chinese Academy of Sciences

To avoid unfavorable environmental conditions, Scrippsiella trochoidea is capable of forming a resting cyst in the process of growth. In the present study,we investigated the effects of nitrate and ammonium on the growth and encystment of S. trochoidea. We incubated *** in modified f/2 media without nitrogen and silicate in flasks. The flasks were divided into two *** was added as a nitrogen source in the first group,and ammonium was added in the second group. The concentrations of the nitrogen compounds were 0, 10, 30, 60,and 90 lmol/L. The results indicate that NO3--N favors cell growth, and the cultures with a higher concentration of NO3--N were ineffective at forming cysts. In contrast,NH4--N promoted cell growth and cyst formation. At similar concentrations as NO3--N, NH4--N had a toxic effect on cell growth and increased the cyst formation ***, the NH4--N concentration is an important factor for controlling encystment. We believe that the impact of NH4--N in inducing cyst formation may be a useful feedback mechanism in ecological systems.

关键词：锥状斯氏藻细胞生长铵态氮硝态氮包囊硝酸溶液氮化合物毒性作用

Distinct root system acclimation patterns of seagrass Zostera japonica in sediments of different trophic status:a research by X-ray computed tomography

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Journal of Oceanology and Limnology 2021年第6期39卷 2267-2280页

作者： Xiaoyue SONG Yi ZHOU Jiangning ZENG Lu SHOU Xiaomei ZHANG Shidong YUE Wei GAO Weihua FENG zhifu wang Ping DU Key Laboratory of Marine Ecosystem Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China College of Tropical Crops Hainan UniversityHaikou 570228China Key Laboratory of Engineering Oceanography Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China

Conspecific seagrass living in differing environments may develop different root system acclimation *** applied X-ray computed tomography(CT)for imaging and quantifying roots systems of Zostera japonica collected from typical oligotrophic and eutrophic sediments in two coastal sites of northern China,and determined sediment physicochemical properties that might influence root system morphology,density,and *** trophic status of sediments had little influence on the *** root length,and diameters of root and ***,*** in oligotrophic sediment developed the root system with longer rhizome node,deeper rhizome distribution,and larger allocation to below-ground tissues in order to acquire more nutrients and relieve the N *** the lower root and rhizome densities of *** in eutrophic sediment were mainly caused by fewer shoots and shorter longevity,which was resulted from the more serious sulfide *** results systematically revealed the effect of sediment trophic status on the phenotypic plasticity,quantity,and distribution of *** root system,and demonstrated the feasibly of X-ray CT in seagrass root system research.

关键词： Zostera japonica root system acclimation pattern sediment trophic status X-ray computed tomography

Research progress of acupuncture analgesia based on autonomic nerve regulation pathway

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Acupuncture and Herbal Medicine 2023年第4期3卷 285-295页

作者： Lina Pang Xiaomei Chen Yanyan Lan Qiuling Huang Xiangmei Yu Lu Qi zhifu wang Department of Acupuncture Chongqing Hospital of Traditional Chinese MedicineChongqingChina School of Rehabilitation Medicine Fujian University of Traditional Chinese MedicineFuzhouChina School of Integrative Medicine Fujian University of Traditional Chinese MedicineFuzhouChina School of Life Sciences Westlake UniversityHangzhouChina Department of Acupuncture Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhouChina

The autonomic nervous system(ANS)includes the sympathetic,parasympathetic,and enteric nervous systems,and its senior regulatory center includes the brainstem,cingulate gyrus,and *** can affect visceral,vascular,and glandular functions via the autonomic nervous regulatory *** this paper,the relationship between pain and autonomic nervous function,the application of acupuncture guided by the autonomic nervous system,and the basis and clinical research on acupuncture analgesia are reviewed.

关键词： Acupuncture Autonomic nervous system Pain Progress