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lncRNA KCNQ1OT1在结肠癌细胞中的促癌功能和下游调控通路研究

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解放军医学院学报 2019年第6期40卷 570-579页

作者：冀天楠赵志飞怀思远张欣悦李建雄解放军总医院第一医学中心放射治疗科

目的明确lncRNA KCNQ1OT1在结肠癌HCT-116和HT-29细胞中的功能,探讨其作用的分子机制。方法在结肠癌HCT-116和HT-29细胞系中转染KCNQ1OT1的过表达质粒及空白对照,siRNA干扰KCNQ1OT1及空白对照;分别在两个细胞系中进行CCK8、划痕实验、Tr... 详细信息

目的明确lncRNA KCNQ1OT1在结肠癌HCT-116和HT-29细胞中的功能,探讨其作用的分子机制。方法在结肠癌HCT-116和HT-29细胞系中转染KCNQ1OT1的过表达质粒及空白对照,siRNA干扰KCNQ1OT1及空白对照;分别在两个细胞系中进行CCK8、划痕实验、Transwell、细胞侵袭实验以验证KCNQ1OT1对细胞功能的影响;免疫共沉淀(RNA immune precipitation,RIP)和双荧光素酶标记实验分别验证KCNQ1OT1与miR-125a-5p的特异结合以及miR-125a-5p和SMURF1蛋白的特异结合。过表达和敲低KCNQ1OT1、miR-125a-5p后,Western hlot验证SMURF1的表达;完成过表达KCNQ1OT1同时过表达miR-125a-5p、敲低KCNQ1OT1同时敲低miR-125a-5p后检测SMURF1表达情况的Resecue实验,验证KCNQ1OT1与miR-125a-5p的调控关系。结果在结肠癌HCT-116和HT-29细胞中,过表达的KCNQ1OT1能促进结肠癌细胞的增殖、迁移和侵袭;KCNQ1OT1敲低后,结肠癌细胞的增殖、迁移和侵袭受抑制;miR-125a-5p与KCNQ1OT1及SMURF1均能特异性结合;过表达KCNQ1OT1促进SMURF1的表达,敲低KCNQ1OT1抑制SMURF1的表达;过表达miR-125a-5p抑制SMURF1的表达,敲低miR-125a-5p促进SMURF1的表达;过表达KCNQ1OT1的基础上同时表达miR-125a-5p能显著抑制SMURF1的表达,敲低KCNQ1OT1的基础上同时敲低miR-125a-5p能促进SMURF1的表达。结论在结肠癌细胞中,lncRNA KCNQ1OT1通过KCNQ1OT1-miR-125a-5p-SMURF1调控轴,促进结肠癌细胞的增殖、迁移和侵袭。

关键词：结肠癌HCT-116、HT-29细胞长链非编码核糖核酸 KCNQ1重叠转录物微小核糖核酸125a-5p E3泛素连接酶SMURF1

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基于主成分分析的普安大厂茶白茶工艺研究

特产研究 2024年第2期46卷 7-15页

作者：杨琦宏李彩云赵支飞牛素贞马安权宋勤飞贵州大学茶学院贵州贵阳550025 沿河土家族自治县农业农村局贵州沿河565300 贵州大学农业生物工程研究院贵州贵阳550025 贵州省马氏白龙古茶研发有限公司贵州普安561500

为挖掘普安大厂茶(Camellia tachangensis ***)的价值,丰富普安茶产品类型,分别以普安大厂茶群体种春梢独芽、一芽二叶、一芽三叶及单叶为原料,参照白茶“萎凋→干燥”的基本工艺设计3种不同萎凋方式制作普安大厂茶白茶。对所制白茶样... 详细信息

为挖掘普安大厂茶(Camellia tachangensis ***)的价值,丰富普安茶产品类型,分别以普安大厂茶群体种春梢独芽、一芽二叶、一芽三叶及单叶为原料,参照白茶“萎凋→干燥”的基本工艺设计3种不同萎凋方式制作普安大厂茶白茶。对所制白茶样品进行感官审评及主要内含成分检测,并通过主成分分析评价其综合品质,以确定普安大厂茶白茶加工的最适原料及工艺参数。结果显示,以独芽原料所制的白茶感官品质较好,表现出鲜醇、花蜜香、甜果香的品质,优于其他嫩度原料。白茶内成分含量在不同嫩度原料及不同萎凋方式下均有差异,但变化没有明显规律。利用白茶感官品质得分、内含成分含量及儿茶素评价指标进行主成分分析,筛选出白茶综合品质最佳的组合为W12,即以独芽为原料,采用“日光自然”复式萎凋所制白茶芽头细长、绿黄带褐较润,汤色绿黄尚亮,清香纯正,滋味鲜甜;茶多酚、氨基酸、咖啡碱、可溶性糖含量较高,水浸出物含量适中,可作为普安大厂茶白茶加工的优选模式。

关键词：普安大厂茶白茶嫩度萎凋方式主成分分析

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超高压碳化硅N沟道IGBT器件的设计与制造

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电子与封装 2022年第4期22卷 10-15页

作者：杨晓磊李士颜赵志飞李赟黄润华柏松南京电子器件研究所宽禁带半导体电力电子器件国家重点实验室南京210016

自主设计和制备了一种耐压超过20 kV的超高压碳化硅(SiC)N沟道绝缘栅双极晶体管(Insulated Gate Bipolar Transistor,IGBT)器件。通过仿真设计优化了器件结构,同时结合自支撑衬底剥离技术、背面激光退火技术以及载流子寿命提升技术,成功... 详细信息

自主设计和制备了一种耐压超过20 kV的超高压碳化硅(SiC)N沟道绝缘栅双极晶体管(Insulated Gate Bipolar Transistor,IGBT)器件。通过仿真设计优化了器件结构,同时结合自支撑衬底剥离技术、背面激光退火技术以及载流子寿命提升技术,成功在N型SiC衬底上制备了SiC N沟道IGBT器件。测试结果表明,该器件阻断电压为20.08 kV时,漏电流为50μA。当栅电极施加20 V电压,集电极电流为20 A时,器件的导通电压为6.0 V,此时器件的微分比导通电阻为27 mΩ·m^(2)。该值仅为15 kV SiC金属氧化物半导体场效应晶体管(Metal-Oxide-Semiconductor Field-Effect Transistor,MOSFET)比导通电阻的1/7,充分显示出SiC N沟道IGBT器件作为双极型器件在高阻断电压、高导通电流密度等方面的突出优势。

关键词：碳化硅 N沟道IGBT 超高压载流子寿命提升技术

Synthesis of Environmentally Friendly Magnesium Linoleate Detergent

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China Petroleum Processing & Petrochemical Technology 2014年第1期16卷 96-100页

作者： Wang Yonglei Li Haiyun Fang Hongxia Ni zhifei zhao Lele School of Chemistry and Chemical Engineering Huangshan University

This paper mainly covers a method for preparing a highly alkaline magnesium linoleate solution with a total base number(TBN) value of 328 mg KOH/g using linoleic acid as the biodegradable raw material, which can substitute for traditional lubricant detergents as an environmentally friendly detergent. Reaction conditions, including the molar ratio of magnesium oxide to linoleic acid, the molar ratio of methanol to magnesium oxide, the carbonation temperature, the molar ratio of water to magnesium oxide, the flow rate of CO2 gas and the duration for injection of CO2 to magnesium oxide system, were optimized.

关键词： lubricant detergent magnesium linoleate high total base number biodegradation

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氢化物发生原子荧光光谱法测定土壤中的锡

资源环境与工程 2012年第4期26卷 412-414,419页

作者：唐兴敏贾正勋彭望吴俊赵志飞方金东湖北省地质实验研究所湖北武汉430034

研究氢化物发生原子荧光光谱法测定土壤样品中的锡,对锡的氢化物发生条件进行详细的实验,确定了锡最优氢化物发生条件;对仪器参数进行了条件实验,选择了最佳仪器工作条件。用土壤国家一级标准物质对分析方法准确度进行了检验,结果准确可... 详细信息

研究氢化物发生原子荧光光谱法测定土壤样品中的锡,对锡的氢化物发生条件进行详细的实验,确定了锡最优氢化物发生条件;对仪器参数进行了条件实验,选择了最佳仪器工作条件。用土壤国家一级标准物质对分析方法准确度进行了检验,结果准确可靠,满足规范要求,分析方法精密度(RSD)为5.2%,分析方法检出限达到0.11μg/g。

关键词：土壤锡原子荧光光谱法

TRIP Steel Retained Austenite Transformation under Cyclic V-bending Deformation

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Journal of Wuhan University of Technology(Materials Science) 2014年第3期29卷 594-600页

作者：李洪洋 ZHANG Yanjing zhao Wenjun GU zhifei LI Xianchao MA Jinlong School of Material Science and Engineering Beijing Institute of Technology National Key Laboratory of Science and Technology on Materials under Shock and Impact Inner Mongolia First Machinery Group Co. Ltd

To investigate the transformation behavior of TRIP steel retained austenite under cyclic load, cyclic V-bending deformation of low carbon Si-Mn TRIP600 was studied by experiment and finite element in this paper. The results showed that, under cyclic V-bending deformation, retained austenite in TRIP steel transformed into martensite gradually with the increasing of bending times, and for the symmetrical characteristic, upper surface and lower surface presented the same transformation tendency. From the first to the fourth V-bending deformatiort, retained austenite volume fraction decreased nearly linearly and then attained saturation step by step. Compressive stress state was helpful for martensite transformation than tension stress state with V-bending deformation, and strain magnitude was the determining factor for retaining anstenite martensitic transformation. With the increasing of bending times effective stress increased and the relationship between maximum effective stress and bending times was nearly linear. Effective stress and effective strain distribution were non-uniform, the maximum effective stress and effective strain were present in the center of the samples. The relationships between retained austenite and V-bending times, and retained austenite with effective strain were set up as Eqs.（1）-（5）. The relationship was typical quadric function, decreased linearly for the initial deformation and attained saturation finally.

关键词： TRIP steel martensitic transformation retained austenite cyclic load

Spatiotemporal variations of deep-sea sediment components and their fluxes since the last glaciation in the northern South China Sea

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Science China Earth Sciences 2017年第7期60卷 1368-1381页

作者： zhao ShaoHua LIU zhifei CHEN Quan WANG XingXing SHI JiangNan JIN HaiYan LIU JingJing JIAN ZhiMin State Key Laboratory of Marine Geology Tongii University Shanghai 200092 China

Sediment components and their fluxes of Cores MD12-3428(water depth: 903 m), MD12-3433(water depth: 2125 m),and MD12-3434(water depth: 2995 m), obtained along a transect on the continental slope of the northern South China Sea, have been conducted to reveal the spatiotemporal variations and the controlling factors of the sediment components and of their *** show that deep-sea sediments in the northern South China Sea are composed mainly of terrigenous(59–89%) and carbonate(6–38%) particles, with minor components of opal(1.6–9.4%) and organic matter(0.7–1.9%). Fluxes of terrigenous and carbonate particles reach up to 2.4–21.8 and 0.4–6.5 g cm–2 kyr–1, respectively, values that are one to two orders of magnitude higher than the fluxes of opal and organic matter. Temporal variations of the percentages and fluxes of deep-sea sediment components have displayed clear glacial-interglacial cyclicity since the last glaciation. Terrigenous, opal, and organic matter percentages and their fluxes increas clearly during marine isotope stage 2, while carbonate percentages and fluxes show an opposite variation pattern or are characterized by an unremarkable increase. This implies that deep-sea carbonate in the South China Sea is affected by the dilution of terrigenous inputs during the sea-level lowstand. With increasing water depth along the transect, the terrigenous percentage increases but with largely decreased fluxes. Both the percentage and flux of carbonate decrease, while the percentages and fluxes of opal and organic matter display much more complicated variational features. The spatiotemporal variations of deep-sea sediment components and of their fluxes since the last glaciation in the northern South China Sea are strongly controlled by sea-level fluctuations. Simultaneously, terrigenous supply associated with monsoonal rainfall, marine primary productivity,and the dilution effect between terrigenous and biogenic particles, also play interconnected roles in th

关键词： Sediment component Accumulation rate Spatiotemporal variation Last glaciation Sea-level fluctuation South China Sea

Cenozoic Vertical-Axis Rotations of the Hoh Xil Basin, Central–Northern Tibet

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Acta Geologica Sinica(English Edition) 2016年第3期90卷 858-869页

作者： RAN Bo zhao Xixi LIU zhifei WANG Chengshan ZHU Lidong JIN Wei LI Yalin State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Chengdu University of TechnologyChengdu 610059China State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092China Department of Earth and Planetary Sciences University of CaliforniaSanta CruzCA 95064USA State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesBeijing 100083China Exploration and Development Research Institute of Daqing Oilfield Company Ltd. Daqing 163712China

Understanding the Cenozoic vertical-axis rotation in the Tibetan Plateau is crucial for continental dynamic evolution. Paleomagnetic and rock magnetic investigations were carried out for the Oligocene and Miocene continental rocks of the Hoh Xii basin in order to better understand the tectonic rotations of central Tibet. The study area was located in the Tongtianhe area located in the southern part of the Hoh Xil basin and northern margin of the Tanggula thrust system in central-northern Tibet. A total of 160 independently oriented paleomagnetic samples were drilled from the Tongtianhe section for this study. The magnetic properties of magnetite and hematite have been recognized by measurements of magnetic susceptibility vs. temperature curves and unbiocking temperatures. The mean directions of the Oligocene Yaxicuo Group in stratigraphic coordinates （Declination/Inclination = 354.9°/29.3°, k = 33.0, a9s = 13.5°, N =5 Sites） and of the Miocene Wudaoliang Group in stratigraphic coordinates （Declination/Inclination = 3.60/36.4°, k = 161.0, a9s = 9.7°, N =3 Sites） pass reversal tests, indicating the primary nature of the characteristic magnetizations. Our results suggested that the sampled areas in the Tuotuohe depression of the Hoh Xil basin have undergone no paleomagnetically detectable rotations under single thrusting from the Tanggula thrust system. Our findings, together with constraints from other tectonic characteristics reported by previous paleomagnetic studies, suggest tectonic rotations in the Cuoredejia and Wudaoliang depressions of the Hoh Xil basin were affected by strike-slip faulting of the Fenghuo Shan-Nangqian thrust systems. A closer examination of geological data and different vertical-axis rotation magnitudes suggest the tectonic history of the Hoh Xil basin may be controlled by thrust and strike-slip faulting since the Eocene.

关键词： Tibetan Plateau Hoh XU basin Vertical-axis rotation Cenozoic

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乳状液膜萃取苯酚废水的研究

江西化工 2022年第3期38卷 55-57页

作者：孙家乐高瞻远王森淼高浩锋赵志菲陆英太原工业学院环境与安全工程系山西太原030008

以NaOH溶液为内水相、煤油为膜相、乳化剂Span 80为表面活性剂,模拟苯酚废水为外水相,利用乳状液膜萃取去除模拟废水中的苯酚。结果表明,当乳化剂Span 80的用量为0.35%、油水比为1:3、内水相NaOH浓度为0.3 mol/L、萃取时间为10 min时,... 详细信息

以NaOH溶液为内水相、煤油为膜相、乳化剂Span 80为表面活性剂,模拟苯酚废水为外水相,利用乳状液膜萃取去除模拟废水中的苯酚。结果表明,当乳化剂Span 80的用量为0.35%、油水比为1:3、内水相NaOH浓度为0.3 mol/L、萃取时间为10 min时,苯酚的去除率最高可达48.74%。苯酚去除率同时受液膜稳定性和乳状液在外水相中分散性的影响,在适宜的乳化条件和萃取条件下才能有效去除苯酚。

关键词：乳状液膜萃取苯酚乳化条件