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ClC-3 chloride channel in hippocampal neuronal apoptosis

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Neural Regeneration Research 2013年第32期8卷 3047-3054页

作者： Lijuan Xu Shuling Zhang Hongling Fan zhichao zhong Xi Li Xiaoxiao Jin Quanzhong Chang Department of Physiology Zhuhai Campus of Zunyi Medical College First Hospital of Putian City Department of Pediatrics Zhuhai Campus of Zunyi Medical College

Over-production of nitric oxide is pathogenic for neuronal apoptosis around the ischemic area fol- lowing ischemic brain injury. In this study, an apoptotic model in rat hippocampal neurons was es- tablished by 0.5 mmol/L 3-morpholinosyndnomine （SIN-l）, a nitric oxide donor. The models were then cultured with 0.1 mmol/L of 4,4＇-diisothiocyanostilbene-2,2＇-disulfonic acid （DIDS; the chloride channel blocker）for 18 hours. Neuronal survival was detected using the 3-（4,5-dimethylthiazol- 2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay, and apoptosis was assayed by Hoechst 33342-labeled neuronal DNA fluorescence staining. Western blot analysis and immunochemilumi- nescence staining were applied to determine the changes of activated caspase-3 and CIC-3 channel proteins. Real-time PCR was used to detect the mRNA expression of CIC-3. The results showed that SIN-1 reduced the neuronal survival rate, induced neuronal apoptosis, and promoted CIC-3 chloride channel protein and mRNA expression in the apoptotic neurons. DIDS reversed the effect of SIN-I. Our findings indicate that the increased activities of the CIC-3 chloride channel may be involved in hippocampal neuronal apoptosis induced by nitric oxide.

关键词： neural regeneration brain injury nitric oxide CIC-3 chloride channel 3-morpholinosyndnomine 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid hippocampal neurons apoptosis grants-supportedpaper neuroregeneration

Effect of minocycline on cerebral ischemia-reperfusion injury

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Neural Regeneration Research 2013年第10期8卷 900-908页

作者： Yuanyin Zheng Lijuan Xu Jinbao Yin zhichao zhong Hongling Fan Xi Li Quanzhong Chang Department of Physiology Zhuhai Campus of Zunyi Medical College Department of Pathology Mindong Hospital of Fujian Medical University Department of Pathology Guangdong Medical College

Minocylcine, a tetracycline derivate, has been shown to cross the blood-brain barrier and enter the central nervous system. In this study, cerebral ischemia-reperfusion injury models were established using the suture method, and minocycline was immediately injected intraperitoneally after cerebral ischemia-repeffusion （22.5 mg/kg, initially 45 mg/kg） at a 12-hour interval. Results showed that after minocycline treatment, the volume of cerebral infarction was significantly reduced, the number of surviving cell in the hippocampal CA1 region increased, the number of apoptotic cells decreased, the expression of caspase-3 and poly（adenosine diphosphate-ribose） polymerase-1 protein was down-regulated, and the escape latency in the water maze test was significantly shortened compared with the ischemia-reperfusion group. Our experimental findings indicate that minocycline can protect against neuronal injury induced by focal ischemia-reperfusion, which may be mediated by the inhibition of caspase-3 and poly（adenosine diphosphate-ribose） polymerase-1 protein expression.

关键词： neural regeneration brain injury minocycline cerebral ischemia-reperfusion hippocampus poly(adenosine diphosphate-ribose) polymerase-1 caspase-3 apoptosis grants-supported paper neuroregeneration

Chloride channel blocker 4,4-diisothiocyanatostilbene-2,2'-disulfonic acid inhibits nitric oxide-induced apoptosis in cultured rat hippocampal neurons

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Neural Regeneration Research 2013年第2期8卷 121-126页

作者： Jinbao Yin Lijuan Xu Shuling Zhang Yuanyin Zheng zhichao zhong Hongling Fan XiLi Quanzhong Chang Department of Physiology Zhuhai Campus of Zunyi Medical College Department of Pathology Dongguan Campus of Guangdong Medical College

Apoptosis in cultured rat hippocampal neurons was induced using the nitric oxide donor 3-morpholinosydnonimine, and cells were treated with the chloride channel blocker, 4,4- diisothiocyanatostilbene-2,2＇-disulfonic acid. Results showed that the survival rate of neurons was significantly increased after treatment with 4,4-diisothiocyanatostilbene-2,2＇-disulfonic acid, and the rate of apoptosis decreased. In addition, the expression of the apoptosis-related proteins poly（adenosine diphosphate-ribose）polymerase-1 and apoptosis-inducing factor were significantly reduced. Our experimental findings indicate that the chloride channel blocker 4,4- diisothiocyanatostilbene-2,2＇-disulfonic acid can antagonize apoptotic cell death of hippocampal neurons by inhibiting the expression of the apoptosis-related proteins poly（adenosine diphosphate-ribose）polymerase-1 and apoptosis-inducing factor.

关键词： neural regeneration brain injury chloride channel 3-morpholinosydnonimine hippocampus poly(adenosine diphosphate-ribose)polymerase-1 apoptosis inducing factor neuronal apoptosis grants-supported paper photographs-containing paper neuroregeneration

Effects of minocycline on learning and memory of mice following ischemic-hypoxic cerebral injuries

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Neural Regeneration Research 2012年第2期7卷 114-118页

作者： Hongling Fan Yuanyin Zheng Lijuan Xu zhichao zhong Shining Cai Shuling Zhang Quanzhong Chang Department of Physiology Zhuhai Campus of Zunyi Medical College Zhuhai 519041 Guangdong Province China

An ischemic-hypoxic animal model was established using right common carotid artery occlusions and inhalation of low concentrations of oxygen in mice. At 10 days after the ischemic-hypoxic injuries, saline-treated mice exhibited significantly prolonged escape latencies in water-maze tests and significantly shorter memory latencies and more mistakes in step-down tests. In contrast, mice treated with 5 mg/kg minocycline exhibited significant reversals of each of these effects compared with the saline-treated control mice. Moreover, we found that minocycline can relieve brain water content and morphological changes in mice following ischemic-hypoxic cerebral injuries. Accordingly, our findings indicate that minocycline provides some protections against the deleterious effects of these injuries in mice.

关键词： minocycline ischemic-hypoxic cerebral injury learning and memory cognition

Micro/nanosystems for controllable drug delivery to the brain

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The Innovation 2024年第1期5卷 139-157页

作者： Mingzhen Tian zhichao Ma Guang-zhong Yang Institute of Medical Robotics School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Drug delivery to the brain is crucial in the treatment for central nervous system *** significant progress has been made in recent years,there are still major challenges in achieving controllable drug delivery to the *** clinical needs arise from various factors,including controlled drug transport,handling large drug doses,methods for crossing biological barriers,the use of imaging guidance,and effective models for analyzing drug *** advances in micro/nanosystems have shown promise in addressing some of these *** include the utilization of microfluidic platforms to test and validate the drug delivery process in a controlled and biomimetic setting,the development of novel micro/nanocarriers for large drug loads across the blood-brain barrier,and the implementation of micro-intervention systems for delivering drugs through intraparenchymal or peripheral *** this article,we present a review of the latest developments in micro/nanosystems for controllable drug delivery to the *** also delve into the relevant diseases,biological barriers,and conventional *** addition,we discuss future prospects and the development of emerging robotic micro/nanosystems equipped with directed transportation,real-time image guidance,and closed-loop control.

关键词： equipped doses barriers

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极端高温处理对鸡冠花种子萌发与生长的影响

中国农学通报 2009年第19期25卷 174-177页

作者：章玉平钟志超广州城市职业学院广州510405

为研究极端高温处理对鸡冠花种子萌发、幼苗生长、发育与变异的影响,采用57～61℃处理鸡冠花的种子,然后播种让其生长、开花,观察其整个生长史的变化。结果表明:高温处理20min内,鸡冠花种子能够忍受的极端温度范围是57～60℃。经过不同... 详细信息

为研究极端高温处理对鸡冠花种子萌发、幼苗生长、发育与变异的影响,采用57～61℃处理鸡冠花的种子,然后播种让其生长、开花,观察其整个生长史的变化。结果表明:高温处理20min内,鸡冠花种子能够忍受的极端温度范围是57～60℃。经过不同高温和不同时间处理的鸡冠花种子,其抗病性都不同程度的下降,易患立枯病。高温处理对花色、花型的变异影响不大。高温处理过的鸡冠花种子萌发后,小苗长势明显比对照的慢,且参差不齐。高温处理未能得到有利的变异。

关键词：鸡冠花种子萌发幼苗生长极端高温变异

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流程图与类C语言实时转换方法

东南大学学报（自然科学版） 2009年第3期39卷 502-507页

作者：钟志超张志胜戴敏曹杰史金飞东南大学机械工程学院南京211189

为了融合图形化和文本语言2种编程方式的优点,提高教育机器人编程平台的直观性和通用性,结合编程平台要求,设计出流程图与类C语言双向实时转换方法.在进行流程图到类C语言转换时,实时根据各个模块间的父子关系遍历模块树,并根据功能模... 详细信息

为了融合图形化和文本语言2种编程方式的优点,提高教育机器人编程平台的直观性和通用性,结合编程平台要求,设计出流程图与类C语言双向实时转换方法.在进行流程图到类C语言转换时,实时根据各个模块间的父子关系遍历模块树,并根据功能模块封装的库函数和语句完成转换,在转换过程中实时进行类C语言规则检查.在进行类C语言到流程图转换时,首先通过词法分析、语法分析和语义分析生成XML代码,再进行统一解析生成流程图.在转换过程中实时进行错误检查,出现错误所在行会高亮显示以提示用户,并给出错误原因以便于用户修改程序.最后,通过典型结构转换实例和应用,验证了所提出方法的有效性和正确性.

关键词：流程图类C语言 XML 实时转换

B_4C/hBN复相陶瓷与球墨铸铁配副摩擦学性能研究

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摩擦学学报 2016年第1期36卷 61-69页

作者：潘武高义民李秀清宋连成钟志超西安交通大学金属强度国家重点实验室陕西西安710049

本文中以热压烧结法制备不同hBN质量分数(0%、10%、20%和30%)的B4C/hBN复相陶瓷作为销试样,与球墨铸铁(QT)盘试样在空气润滑条件下进行销-盘式摩擦磨损试验,滑动速度为0.66 m/s,磨程为850 m,载荷为5 N.借助扫描电子显微镜(SEM)观察摩擦... 详细信息

本文中以热压烧结法制备不同hBN质量分数(0%、10%、20%和30%)的B4C/hBN复相陶瓷作为销试样,与球墨铸铁(QT)盘试样在空气润滑条件下进行销-盘式摩擦磨损试验,滑动速度为0.66 m/s,磨程为850 m,载荷为5 N.借助扫描电子显微镜(SEM)观察摩擦表面和磨屑的形貌特征,利用X光电子能谱(XPS)、X射线衍射(XRD)等分析手段表征磨面和磨屑的物质组成,得出复相陶瓷成分、试验参数对配副摩擦学性能的影响.研究表明:hBN质量分数为0%和10%的复相陶瓷(BN00、BN10)与铸铁配副时,磨损机制以黏着磨损为主,摩擦系数为0.5,h BN质量分数为20%和30%的复相陶瓷(BN20、BN30)与铸铁配副时,陶瓷销表面出现连续的表面膜,该表面膜主要由Fe_2O_3、Fe_3O_4、B_2O_3、B_4C及h BN等物质组成,摩擦系数降低至0.2;随着载荷的升高,QT-BN00与QT-BN10配副磨损机制仍以黏着磨损为主,摩擦系数与5 N载荷无明显变化,而QT-BN20与QT-BN30配副摩擦系数随载荷上升而升高,当载荷为10 N时,摩擦系数升至0.6.

关键词：碳化硼氮化硼摩擦系数表面膜

碳化养护制备钢渣粉3D打印材料及其性能研究

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材料导报 2024年第14期38卷 169-176页

作者：钟旷楠刘志超王发洲武汉理工大学材料科学与工程国际化示范学院武汉430070 武汉理工大学材料科学与工程学院武汉430070 硅酸盐建筑材料国家重点实验室(武汉理工大学) 武汉430070

本工作提出了一种碳化养护钢渣粉3D打印材料的制备方法。以钢渣粉为主要胶凝材料,利用少量普通硅酸盐水泥的高水化活性保证材料打印成型后的初始强度,增强打印材料的可建造性,材料体系中硅灰颗粒的“滚珠效应”改善了打印材料的可挤出性... 详细信息

本工作提出了一种碳化养护钢渣粉3D打印材料的制备方法。以钢渣粉为主要胶凝材料,利用少量普通硅酸盐水泥的高水化活性保证材料打印成型后的初始强度,增强打印材料的可建造性,材料体系中硅灰颗粒的“滚珠效应”改善了打印材料的可挤出性,使用聚乙烯醇(PVA)、羧甲基纤维素钠(CMC)作为流变调节组分进一步调控打印材料的流变性能,满足材料打印性能需求。研究了拌合水固比与流变调节组分掺量对钢渣粉3D打印材料流变性能、打印性能与力学强度的影响规律,探究了碳化养护过程中钢渣粉3D打印材料成分变化与微观结构演变。结果表明,当浆料拌合水固比为0.16,PVA与CMC掺量分别为0.2%(质量分数,下同)、0.1%时,钢渣粉3D打印材料具有最佳的打印性能与力学强度,打印样品经12 h碳化养护后抗压强度为64 MPa,碳化养护过程中,大量镁方解石碳化反应产物填充基体孔隙,迅速提高打印材料基体的致密程度,是打印材料力学性能快速发展的主要机理。

关键词：钢渣粉 3D打印碳化打印性能力学强度