检索结果-南通市图书馆

Differential Gene and Protein Expression in Soybean at Early Stages of Incompatible Interaction with Phytophthora sojae

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2011年第6期10卷 902-910页

作者： LI yong-gang YANG Ming-xiu LI Yan LIU Wen-wen WEN Jing-zhi LI yong-hao Department of Plant Protection Northeast Agricultural University Harbin 150030 P.R.China Department of Plant Pathology and Ecology Connecticut Agricultural Experiment Station 123 Huntington St New Haven CT 06511 USA

Soybean root and stem rot caused by Phytophthora sojae is a destructive disease worldwide. Using genetic resistance is an important and major component in the integrated pest management of this disease. To understand molecular mechanisms of root and stem rot resistance in soybeans, the gene and protein expression in hypocotyls and stems of variety Suinong 10 carrying resistance genes Rps1a and Rps2 was investigated by using mRNA differential display reverse transcription PCR and two-dimensional electrophoresis at 0, 0.5, 1, 2, and 4 h after inoculation with P. sojae race 1. The results of the comparison of gene and protein expression showed that at least eight differential fragments at the transcriptional level were related to metabolic pathway, phytoalexin, and signal transduction in defense responses. Sequence analyses indicated that these fragments represented cinnamic acid 4-hydroxylase gene, ATP b gene coding ATP synthase b subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5 h post inoculation, blue copper protein gene and UDP-N-acetyl-a-D-galactosamine gene which upregulated at 2 h post inoculation, TGA-type basic leucine zipper protein TGA1.1 gene, cyclophilin gene, and 14-3-3 protein gene which upregulated at 4 h post inoculation. Three resistance-related proteins, a-subunit and b-subunit of ATP synthase, and cytochrome P450-like protein, were upregulated at 2 h post inoculation. The results suggested that resistance-related multiple proteins and genes were expressed in the recognition between soybean and P. sojae during zoospore germination, penetration and mycelium growth of P. sojae in soybean.

关键词： Phytophthora sojae resistance mechanism incompatible interaction mRNA differential display reverse transcription PCR two-dimensional electrophoresis

Universal and General Quantum Simultaneous Secret Distribution with Dense Coding by Using One-Dimensional High-Level Cluster States

在线全文

学校读者我要写书评

暂无评论

Journal of Computer Science & Technology 2021年第1期36卷 221-230页

作者： zhi-hao Liu Han-Wu Chen School of Computer Science and Engineering Southeast UniversityNanjing 211189China Key Laboratory of Computer Network and Information Integration(Southeast University) Ministry of Education Nanjing 211189China

A universal and general quantum simultaneous secret distribution(QSSD)protocol is put forward based on the properties of the one-dimensional high-level cluster states,in which one sender dispatches different high-level classical secret messages to many users at the same *** to the idea of quantum dense coding,the sender can send different two-dit classical messages(two d-level classical numbers)to different receivers simultaneously by using a one-dimensional d-level cluster state,which means that the information capacity is up to the *** estimate the security of quantum channels,a new eavesdropping check strategy is put ***,a new attack model,the general individual attack is proposed and *** is shown that the new eavesdropping check strategy can effectively prevent the traditional attacks including the general individual *** addition,multiparty quantum secret report(MQSR,the same as quantum simultaneous secret submission(QSSS))in which different users submit their different messages to one user simultaneously can be gotten if the QSSD protocol is changed a little.

关键词： quantum simultaneous secret distribution(QSSD) multiparty quantum secret report(MQSR) quantum simultaneous secret submission(QSSS) quantum secret sharing quantum broadcast communication

Plugging property and displacement characters of a novel high-temperature resistant polymer nanoparticle

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Petroleum Science 2022年第1期19卷 387-396页

作者： zhi-yong Wang Mei-Qin Lin Huai-Ke Li Zhao-Xia Dong Juan Zhang Zi-hao Yang Unconventional Petroleum Research Institute China University of Petroleum(Beijing)Beijing102249People's Republic of China Oilfield Chemicals R&D Institute China Oilfield Services LimitedLangfang065201People's Republic of China China University of Geosciences(Beijing) Beijing100083People's Republic of China

The goal of the research was to investigate the profile control and oil displacement characteristics of the polymer nanoparticles after high temperature *** displacement parameters showed considerable influence on the plugging effect of the high-temperature swelled polymer nanoparticles,such as the core permeability,concentration of nanoparticles in the suspension,swelling time and swelling temperature,which makes it flexible to control the plugging effect by controlling displacement experiments *** results show that polymer nanoparticles dispersion system with a concentration of 500 mg/L is suitable for cores plugging with a permeability of 30×10^(-3)-150×10^(-3)μm^(2),even after aging at 150℃ for three *** shunt flow experiments show that when the displacement factors are optimal values,the polymer nanoparticles after high temperature swelling to plug the high-permeability layer selectivity and almost do not clog the low-permeability *** recovery of homogeneous artificial core displacement experiment and a heterogeneous double-tube cores model are increased by 20%and 10.4%on the basis of water *** polymer nanoparticles can be a great help for petroleum engineers to better apply this deep profile control and flooding technology.

关键词： Polymer nanoparticles High temperature resistance Plugging property EOR

骨髓间充质干细胞来源的外泌体抑制地塞米松诱导的C2C12肌管萎缩

在线全文

学校读者我要写书评

暂无评论

中国药理学通报 2025年第1期41卷 50-56页

作者：柯义兵丁永宏阿布都克热木·达吾提郭浩然兰志杰王勇平兰州大学第一临床医学院甘肃兰州730000 宁夏医科大学宁夏银川750000 兰州大学第一医院骨科甘肃兰州730000

目的研究骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)来源的外泌体(exsomes,EXOs)对地塞米松(dexamethasone,DEX)诱导的C2C12肌管萎缩的影响。方法(1)分离提取培养C57BL/6J小鼠BMSCs。(2)提取并鉴定BMSCs-EXOs。(3)C2C12细胞成肌分化。(4)将肌管细胞分为对照组(Control,2%马血清培养基培养48 h)、地塞米松组(DEX、浓度为10μmol·L^(-1)的DEX干预肌管48 h)、外泌体组(EXOs、外泌体干预肌管48 h)、外泌体抑制剂组(GW4869、10μmol的GW4869干预BMSCs后提取的外泌体干预肌管48 h)。48 h后测量各组肌管直径,CCK-8法检测各组细胞活力,Western blot检测各组肌肉萎缩及成肌分化相关蛋白的表达量。结果BMSCs呈长梭形,BMSCs-EXOs在透射电镜下为圆形的双层膜结构,直径约200 nm,且高表达CD9、CD63和CD81。相比于Control组,DEX组细胞活性降低(P<0.01),肌管细胞直径减少(P<0.01),atrogin-1(P<0.05)和MuRF-1(P<0.01)的表达明显上调,MYOD(P<0.01)蛋白表达降低;相比于DEX组,BMSCs-EXOs组细胞活性升高(P<0.01),肌管细胞直径增加(P<0.01),atrogin-1(P<0.05)和MuRF-1(P<0.01)的表达明显下调,MYOD(P<0.01)蛋白表达升高;相比于BMSCs-EXOs组,BMSCs-EXOs(GW4869)组细胞活性降低(P<0.05),肌管细胞直径减少(P<0.01),atrogin-1(P<0.05)和MuRF-1(P<0.05)的表达上调,MYOD(P<0.01)蛋白表达降低。结论骨髓间充质干细胞来源的外泌体(BMSCs-EXOs)可抑制地塞米松诱导的C2C12肌管萎缩。

关键词：骨髓间充质干细胞外泌体地塞米松肌管肌肉萎缩 C2C12

AZD1775 synergizes with SLC7A11 inhibition to promote ferroptosis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Life Sciences 2025年第1期 204-218页

作者： Chen Xiong Hong Ling Yingdan Huang Hanzhi Dong Bangxiang Xie Qian hao Xiang Zhou Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences Fudan University Department of Oncology Shanghai Medical CollegeFudan University Department of Breast Surgery Fudan University Shanghai Cancer CenterFudan University Department of Oncology First Affiliated Hospital of Nanchang University Beijing Institute of Hepatology Beijing Youan HospitalCapital Medical University Beijing Engineering Research Center for Precision Medicine and Transformation of Hepatitis and Liver Cancer Key Laboratory of Breast Cancer in Shanghai Fudan University Shanghai Cancer CenterFudan University Shanghai Key Laboratory of Medical Epigenetics International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology)Institutes of Biomedical SciencesFudan University

Tumor suppressor p53-mediated cell cycle arrest and DNA damage repair may exert cytoprotective effects against cancer therapies, including WEE1 inhibition. Considering that p53 activation can also lead to multiple types of cell death, the role of this tumor suppressor in WEE1 inhibitor-based therapies remains disputed. In this study, we reported that nucleolar stress-mediated p53 activation enhanced the WEE1 inhibitor AZD1775-induced ferroptosis to suppress lung cancer growth. Our findings showed that AZD1775 promoted ferroptosis by blocking cystine uptake, an action similar to that of Erastin. Meanwhile, inhibition of WEE1 by the WEE1 inhibitors or si RNAs induced compensatory upregulation of SLC7A11, which conferred resistance to ferroptosis. Mechanistically, AZD1775 prevented the enrichment of H3K9me3, a histone marker of transcriptional repression, on the SLC7A11 promoter by repressing the expression of the histone methyltransferase SETDB1, thereby enhancing NRF2-mediated SLC7A11 transcription. This finding was also validated using the H3K9me3 inhibitor BRD4770. Remarkably, we found that the nucleolar stress-inducing agent Actinomycin D(Act. D) inhibited SLC7A11 expression by activating p53, thus augmenting AZD1775-induced ferroptosis. Moreover, the combination of AZD1775 and Act. D synergistically suppressed wild-type p53-harboring lung cancer cell growth both in vitro and in vivo. Altogether, our study demonstrates that AZD1775 promotes ferroptosis by targeting cystine uptake but also mediates the adaptive activation of SLC7A11 through the WEE1-SETDB1 cascade and NRF2-induced transcription, and inhibition of SLC7A11 by Act. D boosts the anti-tumor efficacy of AZD1775 by enhancing ferroptosis in cancers with wild-type p53.

关键词：

同方期刊数据库

Influence of Pre-deformation on Aging Precipitation Behavior of Three Al–Cu–Li Alloys

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2017年第2期30卷 133-145页

作者： Jin-Feng Li zhi-hao Ye Dan-Yang Liu yong-Lai Chen Xu-Hu Zhang Xiu-zhi Xu Zi-Qiao Zheng School of Materials Science and Engineering Central South University Changsha 410083 China Aerospace Research Institute of Materials and Processing Technology Beijing 100076 China

The influence of pre-deformation on aging precipitates of three near peak-aged Al–Cu–Li alloys,1460 alloy with a low Cu/Li ratio（1.46）,2050 alloy with a high Cu/Li ratio（4.51） and 2A96 alloy with a medium Cu/Li ratio（2.97）,was *** strength of the aged alloys is enhanced by the *** effectiveness of pre-deformation on precipitates is dependent on the alloy＇s *** increasing the pre-deformation,the population density of T1（Al2Cu Li） precipitates increases in all three Al–Cu–Li alloys and their diameter decreases in 2050 and 2A96 alloys,and the greatest effectiveness is observed in 2A96 *** pre-deformation also increases the population density of θ＇（Al2Cu）precipitates and decreases their diameter in 2050 and 2A96 Al–Li alloys,but the effectiveness is smaller compared to that on T1 *** 1460 alloy subjected to two-step aging at 130 ℃ for 20 h followed by 160 ℃ for 12 h,the main precipitates are δ＇（Al3Li）.At 2%–6% pre-deformation,GP-I zones form and pre-deformation displays little *** percentage pre-deformation promotes θ＇＇/θ＇ precipitation and increases their population density.

关键词： Al-Li alloy Pre-deformation Aging Precipitates

100 cm^(2) Organic Photovoltaic Cells with 23%Efficiency under Indoor Illumination

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2022年第8期40卷 979-988,I0011页

作者： yong Cui Hui-Feng Yao Ye Xu Peng-Qing Bi Jian-Qi Zhang Tao Zhang Ling Hong zhi-hao Chen zhi-Xiang Wei Xiao-Tao hao Jian-Hui Hou Laboratory of Polymer Physics and Chemistry Beijing National Laboratory for Molecular SciencesCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100049China CAS key laboratory of nanosystem and hierarchical fabrication CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing100190China School of Physics State Key Laboratory of Crystal MaterialsShandong UniversityJinan250100China

The application of organic photovoltaic(OPV)cells to drive off-grid microelectronic devices under indoor light has attracted broad *** organic semiconductors intrinsically have less ordered intermolecular packing than inorganic materials,the relatively larger energetic disorder is one of the main results that limit the photovoltaic efficiency of the OPV cells at low carrier ***,we optimize the alkyl chains of non-fullerene acceptors to get suppressed energetic *** find the optimal acceptor DTz-R1 with the shortest alkyl chain has the strongest crystalline property and lowest energetic *** a result,over 26%efficiency is recorded for the 1 cm^(2) OPV cells under a light-emitting diode illumination of 500 *** also fabricate a 100 cm^(2) cell device and get a PCE of 23.0%,which is an outstanding value for large-area OPV *** results suggest that modulation of the energetic disorder is of great importance for further improving the efficiency of OPV cells,especially for indoor applications.

关键词： Organic photovoltaic cells Low light intensity Energetic disorder Energy loss Power conversion efficiency

MISCIBILITY,CRYSTALLIZATION AND MECHANICAL PROPERTIES OF PPC/PBS BLENDS

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2007年第6期25卷 589-597页

作者： Hui-liang Zhang Xiao-hong Sun Qing-yong Chen Min-qiao Ren zhi-hao Zhang Hong-fang Zhang zhi-shen Mo State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Graduate School of Chinese Academy of Science Changchun 130022 China

In this paper, melt blends of poly（propylene carbonate）（PPC） with poly（butylene succinate）（PBS） were characterized by dynamic mechanical analysis （DMA）, differential scanning calorimetry （DSC）, tensile testing, wide-angle X-ray diffraction （WAXD）, polarized optical microscopy and thermogravimetric analysis （TGA）. The results indicated that the glass transition temperature of PPC in the 90/10 PPC/PBS blend was decreased by about 11 K comparing with that of pure PPC. The presence of 10% PBS was partially miscible with PPC. The 90/10 PPC/PBS blend had better impact and tensile strength than those of the other PPC/PBS blends. The glass transition temperature of PPC in the 80/20, 70/30, and 60/40 PPC/PBS blends was improved by about 4.9 K, 4.2 K, and 13 K comparing with that of pure PPC, respectively; which indicated the immiscibility between PPC and PBS. The DSC results indicated that the crystallization of PBS became more difficult when the PPC content increased. The matrix of PPC hindered the crystallization process of PBS. While the content of PBS was above 20%, significant crystallization-induced phase separation was observed by polarized optical microscopy. It was found from the WAXD analysis that the crystal structure of PBS did not change, and the degree of crystallinity increased with increasing PBS content in the PPC/PBS blends.

关键词： Poly(propylene carbonate) Poly(butylene succinate) Miscibility Crystallization Mechanical properties.

Optimal squeezed cooling of a mechanical oscillator using measurement-based vector feedback

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2023年第7期66卷 55-61页

作者： zhi-Cheng Gong Cheng-Yu Shen Tian-Hua Mao Chang-Pu Sun yong Li hao Fu School of Science Hainan UniversityHaikou 570228China Innovation Academy for Precision Measurement Science and Technology Chinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100190China Graduate School of China Academy of Engineering Physics Beijing 100193China Beijing Computational Science Research Center Beijing 100193China Center for Theoretical Physics Hainan UniversityHaikou 570228China

Measurement-based feedback has been adopted as an efficient cooling scheme capable of suppressing the thermal fluctuation of a mechanical resonator to a level limited by measurement *** present a scheme of squeezed cooling in which the limit of feedback cooling imposed by the measurement imprecision is surpassed by parametrically squeezing the mechanical resonator in a periodically oscillating optical *** stabilize the parametrically amplified quadrature and simultaneously optimize the cooling for the squeezed quadrature,a measurement-based vector feedback capable of independently controlling the orthogonal motional quadrature is *** experiment demonstrates that the optimal condition for the squeezed cooling closely depends on the strength of *** the ability to keep the orthogonal quadrature well decoupled,optimal squeezed cooling of the mechanical resonator under strong squeezing is achieved with the squeezed variance becoming 8.1 dB below the limit of feedback cooling and the creation of a 31.7 dB thermomechanical squeezed *** this research is conducted in the classical regime,the scheme of squeezed cooling can be naturally extended to quantum systems with the prospects of creating quantum squeezing of a room-temperature mechanical resonator and enabling displacement measurement beyond the standard quantum limit.

关键词： squeezed cooling measurement-based feedback parametric squeezing optimal control optomechanics