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一种大容量燃油箱渗漏测试方法及装置

移动电源与车辆 2024年第2期56卷 20-23+29页

作者：刘伟东张芮张龙关敏岳炜明张志栋兰州电源车辆研究所有限公司甘肃省内燃机电站工程技术研究中心兰州中瑞电源产品质量检验有限公司

根据某产品燃油箱渗漏问题，研究开发了一种大容量燃油箱渗漏测试方法及装置。该测试方法可有效缩短油箱检测工序时间，简化测试流程，降低测试成本，优化大容量燃油箱渗漏检测的方法。

关键词：大容量燃油箱渗漏工艺测试方法

Electrostatic removal of ultrafine carbon black with square-grooved collecting plates:Insights for capturing black carbon emitted from ships

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Journal of Environmental Sciences 2024年第2期136卷 559-569页

作者： zhicheng Wu YifanWang Lingyu Shao Hao zhang Haobo Dai Haidong Fan Chenghang Zheng Xiang Gao State Key Lab of Clean Energy Utilization State Environmental Protection Engineering Center for Coal-Fired Air Pollution ControlZhejiang UniversityHangzhou 310027China Department of Earth and Environmental Sciences University of ManchesterOxford RoadUnited Kingdom Zhejiang Tiandi Environmental Protection Engineering Co. Ltd.Hangzhou 310012China Baima Lake Laboratory(Zhejiang Provincial Laboratory of Energy and Carbon Neutrality) Hangzhou 310000China

Ships and other mobile pollution sources emitted massive ultrafine and low-resistivity particles containing black carbon(BC),which were harmful to human health and were difficult to capture by conventional electrostatic precipitators(ESPs).In this study,nanoscale carbon black was adopted as simulated particles(SP)with similar physicochemical properties for black carbon emitted from ships(SP-BC)to investigate the feasibility of using an ESP with square-grooved collecting plates for the removal of SP-BC at low *** increased applied voltage significantly improved the total collection of SP-BC whereas may also promote the conversion of relatively larger particle size SP-BC into nano-size below *** outlet number concentration of SP-BC under 27 kV at 130℃was three times that of the *** the reduction of the flow rate could strengthen the capture of SP-BC below20 nm,and under the combined action of low flow rate and maximum applied voltage,the collection efficiency of 20-100 nm SP-BC could exceed 90%.In addition,the escape and capture characteristics of SP-BC under long-term rapping were *** square-grooved collecting plate could effectively restrain the re-entrainment of collected SP-BC generated by rapping,and the nanoscale SP-BC was trapped in the grooves after *** results could provide insights into the profound removal of massive nanoscale black carbon emissions from mobile sources.

关键词： Simulated ship black carbon Electrostatic removal Square-grooved collecting plate Long-term rapping

A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

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The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw zhijun Tong Yirong Yang zhiwei Wang Liying zhang Lihua Deng zhiguo zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

毛乌素沙地樟子松人工林根内真菌群落结构与功能时间动态特征

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环境科学 2024年第7期45卷 4241-4250页

作者：任悦高广磊丁国栋张英赵珮杉北京林业大学水土保持学院北京100083 林木资源高效生产全国重点实验室北京100083 宁夏盐池毛乌素沙地生态系统国家定位观测研究站盐池751500 林业生态工程教育部工程研究中心北京100083 水土保持国家林业和草原局重点实验室北京100083

为揭示毛乌素沙地樟子松人工林根内真菌群落结构和功能时间动态特征,以毛乌素沙地不同林龄(23、33和44 a)樟子松人工林为研究对象,采用高通量测序技术,比较分析不同月份(4~9月)樟子松人工林根内真菌群落组成及其对环境因子的响应.结果表... 详细信息

为揭示毛乌素沙地樟子松人工林根内真菌群落结构和功能时间动态特征,以毛乌素沙地不同林龄(23、33和44 a)樟子松人工林为研究对象,采用高通量测序技术,比较分析不同月份(4~9月)樟子松人工林根内真菌群落组成及其对环境因子的响应.结果表明:(1)樟子松根内真菌群落分布存在明显的季节性,月份对樟子松根内真菌多样性指数影响显著(P<0.05),在5月和7月较高;樟子松根内真菌多样性指数随林龄的增大而逐渐减小,林龄对其的影响不显著.(2)毛乌素沙地樟子松根内真菌优势菌门为子囊菌门(Ascomycota);不同营养型真菌相对丰度随月份变化,优势类群为腐生-共生营养型真菌、未定义腐生菌和外生菌根真菌;5月、7月和9月的指示外生菌根真菌分别为囊蘑属(Melanoleuca)、缘腺革菌属(Amphinema)和口蘑属(Tricholoma).(3)毛乌素沙地樟子松根内真菌群落分布受年均相对湿度、年均降水量、土壤孔隙度、土壤铵态氮、年均日照时数、年均温和土壤含水量的显著影响(P<0.05),指示菌种主要受土壤有机碳含量、孔隙度、年均降水量和年均空气湿度的影响.气候和土壤性质等环境因子的变化塑造了毛乌素沙地樟子松人工林根内真菌群落结构和功能的时间动态特征,而林龄的贡献较小.研究结果可为樟子松人工林可持续经营管理提供理论依据.

关键词：樟子松根内真菌生态功能环境因子林龄

Online hierarchical recognition method for target tactical intention in beyond-visual-range air combat

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Defence Technology（防务技术） 2022年第8期18卷 1349-1361页

作者： Zhen Yang zhi-xiao Sun Hai-yin Piao Ji-chuan Huang De-yun Zhou zhang ren School of Automation Science and Electrical Engineering Beihang UniversityBeijing100191China Northwestern Polytechnical University Xi'an710072China The First Military Representative Office of the Air Force Equipment Department in Chengdu Chengdu610091China

Online accurate recognition of target tactical intention in beyond-visual-range (BVR) air combat is an important basis for deep situational awareness and autonomous air combat decision-making, which can create pre-emptive tactical opportunities for the fighter to gain air superiority. The existing methods to solve this problem have some defects such as dependence on empirical knowledge, difficulty in interpreting the recognition results, and inability to meet the requirements of actual air combat. So an online hierarchical recognition method for target tactical intention in BVR air combat based on cascaded support vector machine (CSVM) is proposed in this study. Through the mechanism analysis of BVR air combat, the instantaneous and cumulative feature information of target trajectory and relative situation information are introduced successively using online automatic decomposition of target trajectory and hierarchical progression. Then the hierarchical recognition model from target maneuver element, tactical maneuver to tactical intention is constructed. The CSVM algorithm is designed for solving this model, and the computational complexity is decomposed by the cascaded structure to overcome the problems of convergence and timeliness when the dimensions and number of training samples are large. Meanwhile, the recognition result of each layer can be used to support the composition analysis and interpretation of target tactical intention. The simulation results show that the proposed method can effectively realize multi-dimensional online accurate recognition of target tactical intention in BVR air combat.

关键词： Beyond-visual-range(BVR)air combat Tactical intention recognition Hierarchical recognition model Cascaded support vector machine(CSVM) Trajectory decomposition Maneuver element

Damage Mechanism of Ultra-thin Asphalt Overlay(UTAO) based on Discrete Element Method

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Journal of Wuhan University of Technology(Materials Science) 2024年第2期39卷 473-486页

作者：杜晓博 GAO Liang RAO Faqiang 林宏伟 zhang Hongchao SUN Mutian XU Xiuchen Postdoctoral Station of Mechanical Engineering Tongji UniversityShanghai 201804China Guangzhou Daguang Expressway Co. LtdGuangzhou 510075China Key Laboratory of Road and Traffic Engineering of Ministry of Education Tongji UniversityShanghai 201804China

Aiming to analyze the damage mechanism of UTAO from the perspective of meso-mechanical mechanism using discrete element method(DEM),we conducted study of diseases problems of UTAO in several provinces in China,and found that aggregate spalling was one of the main disease types of UTAO.A discrete element model of UTAO pavement structure was constructed to explore the meso-mechanical mechanism of UTAO damage under the influence of layer thickness,gradation,and bonding *** experimental results show that,as the thickness of UTAO decreasing,the maximum value and the mean value of the contact force between all aggregate particles gradually increase,which leads to aggregates more prone to *** with OGFC-5 UTAO,AC-5 UTAO presents smaller maximum and average values of all contact forces,and the loading pressure in AC-5 UTAO is fully diffused in the lateral *** addition,the increment of pavement modulus strengthens the overall force of aggregate particles inside UTAO,resulting in aggregate particles peeling off more *** increase of bonding modulus changes the position where the maximum value of the tangential force appears,whereas has no effect on the normal force.

关键词： ultra-thin asphalt overlay pavement distress discrete element method meso-mechanics damage mechanism

Effects of traditional Chinese medicine on treatment outcomes in severe COVID-19 patients:a single-centre study

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Chinese Journal of Natural Medicines 2024年第1期22卷 89-96页

作者： XIAO Yongjiu LI Binbin LIU Chang HUANG Xiuyu MA Ling QIAN zhirong zhang Xiaopeng zhang Qian LI Dunqing CAI Xiaoqing YAN Xiangyong LUO Shuping XIANG Dawei XIAO Kun Emergency Department the 940th Hospital of Jiont Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Scientific Research Center the Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518000China School of Medicine Nankai universityTianjin 300000China Department of Cardiology the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Department of Radiation Oncology Fujian Medical University Union HospitalFuzhou 350000China Department of Traditional Chinese Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Department of Geriatric Diseases the Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China Health and Sport Administration Muskingum UniversityOhioanUSA Laboratory of Vaccine and Antibody Engineering Beijing Institute of BiotechnologyBeijing 100071China Department of Pulmonary and Critical Care Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Center of Pulmonary&Critical Care Medicine Chinese People’s Liberation Army(PLA)General HospitalBeijing 100853China

As the search for effective treatments for COVID-19 continues,the high mortality rate among critically ill patients in Intensive Care Units(ICU)presents a profound *** study explores the potential benefits of traditional Chinese medicine(TCM)as a supplementary treatment for severe COVID-19.A total of 110 critically ill COVID-19 patients at the Intensive Care Unit(ICU)of Vulcan Hill Hospital between Feb.,2020,and April,2020(Wuhan,China)participated in this observational *** patients received standard supportive care protocols,with a subset of 81 also receiving TCM as an adjunct *** characteristics during the treatment period and the clinical outcome of each patient were closely monitored and *** findings indicated that the TCM group exhibited a significantly lower mortality rate compared with the non-TCM group(16 of 81 vs 24 of 29;0.3 vs 2.3 person/month).In the adjusted Cox proportional hazards models,TCM treatment was associated with improved survival odds(P<0.001).Furthermore,the analysis also revealed that TCM treatment could partially mitigate inflammatory responses,as evidenced by the reduced levels of proinflammatory cytokines,and contribute to the recovery of multiple organic functions,thereby potentially increasing the survival rate of critically ill COVID-19 patients.

关键词： COVID-19 Pneumonia Novel coronavirus Critically ill Traditional Chinese medicine Clinical outcome

Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

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Journal of Integrative Agriculture 2024年第5期23卷 1494-1506页

作者： Xiaohui Wu Mengyuan zhang Zheng Zheng Ziqi Sun Feiyan Qi Hua Liu Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Xiao Wang Hongfei Liu Wenzhao Dong Xinyou zhang College of Agronomy Henan Agricultural UniversityZhengzhou 450046China Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop ImprovementZhengzhou 450002China The Shennong Laboratory Zhengzhou 450002China Shangqiu Academy of Agricultural and Forestry Sciences Shangqiu 476000China

Peanut(Arachis hypogaea L.)is a globally important oil *** blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses *** web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web *** the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2:3 segregating population and identify candidate loci related to web blotch *** on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome *** region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two *** markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

关键词： peanut web blotch bulked segregant analysis KAsP markers resistant gene

Genome-wide association with transcriptomics reveals a shade-tolerance gene network in soybean

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The Crop Journal 2024年第1期12卷 232-243页

作者： Yanzhu Su Xiaoshuai Hao Weiying Zeng Zhenguang Lai Yongpeng Pan Can Wang Pengfei Guo zhipeng zhang Jianbo He Guangnan Xing Wubin Wang Jiaoping zhang Zudong Sun Junyi Gai Soybean Research Institute/MARA National Center for Soybean Improvement MARA Key Laboratory of Biology and Genetic Improvement of Soybean/State Key Laboratory for Crop Genetics and Germplasm Enhancement/State Innovation Platform for Integrated Production and Education in Soybean Bio-breeding/Jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing Agricultural UniversityNanjing 210095JiangsuChina Guangxi Academy of Agricultural Sciences Nanning 530007GuangxiChina

Shade tolerance is essential for soybeans in inter/relay cropping systems.A genome-wide association study(GWAS)integrated with transcriptome sequencing was performed to identify genes and construct a genetic network governing the trait in a set of recombinant inbred lines derived from two soybean parents with contrasting shade *** improved GWAS procedure,restricted two-stage multi-locus genome-wide association study based on gene/allele sequence markers(GASM-RTM-GWAS),identified 140 genes and their alleles associated with shade-tolerance index(STI),146 with relative pith cell length(RCL),and nine with *** of these genes by biological categories allowed the construction of a protein–protein interaction network by 187 genes,of which half were differentially expressed under shading and non-shading conditions as well as at different growth *** the identified genes,three ones jointly identified for both traits by both GWAS and transcriptome and two genes with maximum links were chosen as beginners for entrance into the ***,both STI and RCL gene systems worked for shade-tolerance with genes interacted each other,this confirmed that shadetolerance is regulated by more than single group of interacted genes,involving multiple biological functions as a gene network.

关键词： Soybean(Glycine max(L.)Merr.) Shade-tolerance Restricted two-stage multi-locus genomewide association study based on gene/allele sequence markers(GASM-RTM-GWAS) Shade-tolerance index(STI) Relative cell length(RCL) Transcriptome