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Abundance and Diversity of RuBisCO Genes Responsible for CO_2 Fixation in Arid Soils of Northwest China

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Pedosphere 2015年第1期25卷 150-159页

作者： tang zhi-Xi FAN Fen-Liang WAN Yun-Fan WEI Wei LAI Li-Ming State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Ministry of Agriculture Key Laboratory of Agro-Environment and Climate Change Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural Sciences

Arid soils where water and nutrients are scarce occupy over 30% of the Earth's total surface. However, the microbial autotrophy in the harsh environments remains largely unexplored. In this study, the abundance and diversity of autotrophic bacteria were investigated, by quantifying and profiling the large subunit genes of ribulose-1,5-bisphosphate carboxylase/oxygenase(Ru Bis CO) form I(cbb L) responsible for CO2 fixation, in the arid soils under three typical plant types(Haloxylon ammodendron, Cleistogenes chinensis,and Reaumuria soongorica) in Northwest China. The bacterial communities in the soils were also characterized using the 16 S r RNA gene. Abundance of red-like autotrophic bacteria ranged from 3.94 × 105 to 1.51 × 106 copies g-1dry soil and those of green-like autotrophic bacteria ranged from 1.15 × 106 to 2.08 × 106 copies g-1dry soil. Abundance of both red- and green-like autotrophic bacteria did not significantly differ among the soils under different plant types. The autotrophic bacteria identified with the cbb L gene primer were mainly affiliated with Alphaproteobacteria, Betaproteobacteria and an uncultured bacterial group, which were not detected in the 16 S r RNA library. In addition, 25.9% and 8.1% of the 16 S r RNA genes were affiliated with Cyanobacteria in the soils under H. ammodendron and R. soongorica, respectively. However, no Cyanobacteria-affiliated cbb L genes were detected in the same soils. The results suggested that microbial autotrophic CO2 fixation might be significant in the carbon cycling of arid soils, which warrants further exploration.

关键词： autotrophic bacteria carbon cycling cbb L harsh environments real-time polymerase chain reaction

Contralateral S1 function is involved in electroacupuncture treatment-mediated recovery after focal unilateral M1 infarction

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Neural Regeneration Research 2022年第6期17卷 1310-1317页

作者： Lu-Lu Yao Si Yuan Zhen-Nan Wu Jian-Yu Luo Xiao-Rong tang Chun-zhi tang Shuai Cui Neng-Gui Xu South China Research Center for Acupuncture and Moxibustion Medical College of Acupuncture Moxibustion and RehabilitationGuangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Research Institute of Acupuncture and Meridian Anhui University of Chinese MedicineHefeiAnhui ProvinceChina

Acupuncture at acupoints Baihui(GV20)and Dazhui(GV14)has been shown to promote functional recovery after ***,the contribution of the contralateral primary sensory cortex(S1)to recovery remains *** this study,unilateral local ischemic infarction of the primary motor cortex(M1)was induced by photothrombosis in a mouse ***(EA)was subsequently performed at acupoints GV20 and GV14 and neuronal activity and functional connectivity of contralateral S1 and M1 were detected using in vivo and in vitro electrophysiological recording *** results showed that blood perfusion and neuronal interaction between contralateral M1 and S1 is impaired after unilateral M1 *** neuronal excitability and activity were also disturbed,which was rescued by ***,the effectiveness of EA treatment was inhibited after virus-mediated neuronal ablation of the contralateral *** conclude that neuronal activity of the contralateral S1 is important for EA-mediated recovery after focal M1 *** study provides insight into how the S1-M1 circuit might be involved in the mechanism of EA treatment of unilateral cerebral *** animal experiments were approved by the Committee for Care and Use of Research Animals of Guangzhou University of Chinese Medicine(approval No.20200407009)April 7,2020.

关键词： brain plasticity electroacupuncture electrophysiology recording neuronal activity primary motor cortex primary sensory cortex stroke

Identification of in vitro and in vivo metabolites of 12β-hydroxylveratroylzygadenine associated with neurotoxicity by using HPLC–MS/MS

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Chinese Chemical Letters 2014年第8期25卷 1107-1111页

作者： Yue Cong Jing-Gong Guo zhi tang Qing-Chun Zhang Zong-Wei Cai Institute of Pharmacy Pharmaceutical College Henan University State Key Laboratory of Environmental and Biological Analysis Department of Chemistry Hong Kong Baptist University The Key Laboratory of Plant Stress Biology Henan University

Metabolism study was carried out on 12b-hydroxylveratroylzygadenine（VOG） that is a cevine-type alkaloid existing in Veratrum nigrum L. and a neurotoxic component. In order to better understand the potential mechanism of neurotoxicity of VOG, this study measured VOG-induced DNA damage in the cerebellum and cerebral cortex of mice after 7 days repetitive oral dose by using single-cell gel electrophoresis（Comet assay）. High performance liquid chromatography-tandem mass spectrometry（LC–MS/MS） was developed and applied to separate and identify in vitro and in vivo metabolites of VOG for investing the possible relationship of metabolism and neurotoxicity. In vitro experiment was carried out using rat liver microsomes, while the in vivo study was conducted on rats. The obtained results indicated that VOG might cause DNA damage in cerebellum and cerebral cortex of mice in a dosedependent manner. Hydrolysis of ester bond and O-demethylation were proposed to be the main in vivo metabolic pathways of VOG, while the major in vitro metabolic pathways were proposed as methyl oxidation to aldehyde, dehydrogenation, hydrolysis of ester bond, hydrolysis of ester bond together with acetylation, and methoxylation. O-Demethylation reaction was likely to be associated with reactive oxygen species production, leading to the DNA damage.

关键词： 12β-Hydroxylveratroylzygadenine Metabolites O-Demethylation LC-MS

Systems pharmacology approach reveals the antiinflammatory effects of Ampelopsis grossedentata on dextran sodium sulfate-induced colitis

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World Journal of Gastroenterology 2018年第13期24卷 1398-1409页

作者： You-Lan Chen Ya-Li Zhang Yan-Cheng Dai zhi-Peng tang Institute of Digestive Disease Longhua Hospital Shanghai University of Traditional Chinese Medicine Department of Gastroenterology Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

AIM To investigate the protective effects of Ampelopsis grossedentata(AMP) on dextran sulfate sodium(DSS)-induced colitis in mice based on systems pharmacology *** Systems pharmacology approach was used to predict the active ingredients, candidate targets and the efficacy of AMP on ulcerative colitis(UC) using a holistic process of active compound screening, target fishing, network construction and analysis. A DSSinduced colitis model in C57 BL/6 mice(n = 10/group) was constructed and treated with 5-aminosalicylic acid(100 mg/kg/d) and AMP(400 mg/kg/d) to confirm the underlying mechanisms and effects of AMP on UC with western blot analyses, polymerase chain reaction, histological staining and *** The therapeutic effects of AMP against DSS-induced colitis were determined in the beginning, and the results showed that AMP significantly improved the disease in general observations and histopathology analysis. Subsequent systems pharmacology predicted 89 corresponding targets for the four candidate compounds of AMP, as well as 123 candidate targets of UC, and protein-protein interaction networks were constructed for the interaction of putative targets of AMP against UC. Enrichment analyses on TNF-α and RANKL/RANK, a receptor activator of NF-κB signaling pathways, were then carried out. Experimental validation revealed that inflammation-related signaling pathways were activated in the DSS group, and AMP significantly suppressed DSS-induced high expression of IRAK1, TRAF6, IκB and NF-κB, and inhibited the elevated expression levels of TNF-α, IL-1β, IL-6 and *** AMP could exert protective effects on UC via suppressing the IRAK1/TRAF6/NF-κB-mediated inflammatory signaling pathways.

关键词： Ulcerative colitis Ampelopsis grossedentata Traditional Chinese medicine Systems pharmacology Inflammation

Experimental Study on the Treatment of Tertiary Oil Recovery Wastewater via a Novel Electro-Coagulation Method

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Fluid Dynamics & Materials Processing 2023年第1期19卷 51-60页

作者： Wei Cui zhilun Yan zhi tang Mengyao Xu Jian Tian Chengyi Shen Petroleum Engineering Technology Research Institute of Sinopec Northwest Oilfield Company Urumchi830011China Key Laboratory of Enhanced Oil Recovery in Carbonate Fractured-Vuggy Reservoirs Urumchi830011China

At present,methods for treating tertiary oil recovery wastewater via electro-coagulation are still in their early stage of *** this study,a device for electro-coagulation wastewater treatment was built and tested in an oil *** effects that the initial pH value,electrode type,and connection mode have on the coagulation and separation effect were assessed by measuring the mass fraction and turbidity of *** results have shown that when the electro-coagulation method is used,the effectiveness of the treatment can be significantly increased in neutral pH conditions(pH=7),in acidic conditions,and in alkaline *** to an Al electrode,the floc that is produced by an Fe electrode is smaller;thus,it does not easily coagulate and settle in a short *** the oil removal rate,turbidity removal rate and energy consumption as a basis to assess the performances,the results have demonstrated that the combined aluminum alloy iron composite electrode should be used as electrolytic electrode.

关键词： Electrocoagulation oily sewage polymer experiment study

EXPRESSION OF A TELOMERASE-ASSOCIATED GENE IN NORMAL, ATROPHIC ,AND TUMOROUS TESTES

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Chinese Medical Sciences Journal 2005年第3期20卷 217-220页

作者： Fang Mei Bo Zhang zhi-wei tang Lin Hou Department of Pathology Health Science Center Peking University Beijing 100083

Objective To evaluate the expression of telomerase transcriptional elements-interacting factor （TEIF） in human testis under different status and its relation with human telomerase reverse transcriptase （hTERT） expression. Methods Specific antisera against TEIF were generated by immunization of rabbits with purified recombinated partial TEIF. Samples were assigned to three groups according to their pathological types, including 16 normal testes, 8 atrophic testes, and 6 testicular seminomas. They were subjected to immunohistochemical staining of TEIF and hTERT. Results from both TEIF and hTERT were analyzed semi-quantitatively and compared. The expressions of TEIF and hTERT were detected in all samples of normal, atrophic testes, and seminomas. No differences of TEIF expressions among these three groups were observed （P 〉 0.05）. On the contrary, the expressions of hTERT were significantly lower in atrophic testes compared with those of normal testes and seminomas （both P 〈 0.05）. Nevertheless, co-expressions of TEIF with hTERT were revealed to be in normal and malignant cases （P 〈 0.05） but not in atrophic testes, which generally presented TEIF expression. The cellular distributions of both proteins were similar and mainly in spermatocytes and some Sertoli cells, while were all negative in the interstitial cells and other stromal cells. Conclusion The uniform expressions of TEIF in all these specimens suggest that it may be a marker of testis and its related diseases. The strong expression of hTERT in normal testes and testicular seminomas comparing with the low expression in atrophic testes may suggest a role for telomerase in maintaining proliferation of germ cells.

关键词： telomerase transcriptional elements-interacting factor human telomerase reverse transcriptase immunohistochemistry testis

Prognostic role of serum carcinoembryonic antigen in patients receiving liver resection for colorectal cancer liver metastasis:A meta-analysis

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World Journal of Gastrointestinal Surgery 2023年第12期15卷 2890-2906页

作者： Fan tang Cheng-Wen Huang zhi-Hong tang Shao-Long Lu Tao Bai Qing Huang Xing-zhi Li Bin Zhang Fei-Xiang Wu Department of Hepatobiliary Surgery Guangxi Medical University Cancer HospitalNanning 530021Guangxi Zhuang Autonomous RegionChina Key Laboratory of High-Incidence-Tumor Prevention&Treatment Ministry of EducationNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Carcinoembryonic antigen(CEA)is a broad-spectrum tumor marker for differential diagnosis,monitoring,and response assessment of a variety of *** To evaluate whether serum CEA could predict the prognosis in patients with colorectal cancer liver metastasis(CRCLM)before and after liver resection(LR).METHODS PubMed,Embase,Cochrane,and Web of Science were systematically searched to retrieve literature,with a search cut-off date of February 27,*** were strictly screened for inclusion according to pre-specified inclusion and exclusion *** were pooled and analyzed using Stata *** This meta-analysis included 36 studies involving a total of 11143 CRCLM *** results showed that a high pre-LR serum CEA level was correlated with poor overall survival(OS)[hazard ratio(HR)=1.61,95%confidence interval(CI):1.49-1.75,P<0.001]and recurrence-free survival(HR=1.27,95%CI:1.11-1.45,P<0.001)in CRCLM patients.A high post-LR serum CEA level predicted poor OS(HR=2.66,95%CI:2.10-3.38,P<0.001).A comparison by treatment modality,analysis modality,patient source,and cutoff-value showed that overall,high preoperative and postoperative serum CEA levels remained correlated with a poor *** This study concluded that high pre-LR and post-LR serum CEA levels were significantly correlated with a poor prognosis in CRCLM patients.

关键词： Carcinoembryonic antigen Colorectal cancer liver metastasis Liver resection Meta-analysis

Effect of Cations on the Chemical Mechanical Polishing of SiO_(2) Film

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Chinese Physics Letters 2013年第9期30卷 173-176页

作者： SONG Han WANG Liang-Yong LIU Wei-Li SONG zhi-tang State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050

We investigate the effect of cations with different valences on the chemical mechanical polishing(CMP)of silicon dioxide *** removal rate and surface roughness of the silicon-dioxide-film post-CMP are checked for the silica-based slurry with different cation salts(NaCl,CaCl_(2),AlCl_(3)).Meanwhile,the particle size and size distribution of the slurries are characterized to test their *** result shows that the three kinds of salts can improve the polishing removal rate from around 20nm/min to 120nm/min without affecting the surface roughness when the polishing slurry is *** increasing valence of cations,the polishing slurry requires less cation concentration to be added to improve the removal rate,while keeping a superior surface topography and maintaining a longer lifetime as well.

关键词： polishing roughness dioxide

Synthesis and Properties of Double Calix[4]arene Derivatives Linked by Schiff-base in the Lower Rim

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Chinese Chemical Letters 1999年第7期10卷 539-542页

作者： Hu Shan YUAN zhi tang HUANG(Institute of Chemistry The Chinese Academy of Sciences. Beijing 100080) Chinese Acad Sci Inst Chem Beijing 100080 Peoples R China

25,27-bis-(2-aminoethoxy)-p-tert-butylcalix[4]arene was linked to double calix[4]arene derivatives by the Schiff-base moiety in its lower rim. They behave strong ability to complex with Ph2+, Cu2+ and Co2+.

关键词： 25,27-bis-(2-aminoethoxy)-p-tert-butylcalix arenes Schiff-base double calixarene