检索结果-南通市图书馆

Functions of the adaptor protein p66shc in solid tumors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers in Biology 2015年第6期10卷 487-494页

作者： Yanan Sun Jie Yang zhenyi ma Department of Biochemistry and Molecular Biology School of Basic Medical Sciences Tianjin Medical University Tianjin 300070 China

p66she is a 66 kDa Src homology 2 domain containing （Shc） adaptor protein homolog. Previous studies have demonstrated that p66she is a proapoptotic protein involved in the cellular response to oxidative stress and in regulating mammalian lifespan. However, accumulating evidence also indicates its critical role in solid tumor progression. The expression of p66she varies in different types of solid tumors, and it can paradoxically promote as well as suppress tumor progression, survival, and metastasis, depending on the cellular context. In this review, we discuss its functions in various solid tumors, the mechanisms by which it mediates the process of anoikis （detachment-induced cell death）, and the epigenetic mechanisms that regulate its expression. These studies indicate the potential of p66she as a novel prognostic marker and therapeutic target for the prevention of tumor progression and metastasis.

关键词： adaptor protein p66she anoikis metastasis autophagy

Single-cell RNA-seq reveals the transcriptional program underlying tumor progression and metastasis in neuroblastoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2024年第4期18卷 690-707页

作者： Zhe Nian Dan Wang Hao Wang Wenxu Liu zhenyi ma Jie Yan Yanna Cao Jie Li Qiang Zhao Zhe Liu Department of Immunology School of Basic Medical SciencesTianjin Medical UniversityTianjin300070China Zhejiang Key Laboratory of Medical Epigenetics Department of Cell BiologySchool of Basic Medical SciencesHangzhou Normal UniversityHangzhou311121China Department of Pediatric Oncology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin’s Clinical Research Center for CancerTianjin300060China Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical UniversityNanjing211166China

Neuroblastoma(NB)is one of the most common childhood *** percent of patients present with widely disseminated clinical signs at diagnosis and exhibit poor ***,the molecular mechanisms triggering NB metastasis remain largely *** this study,we generated a transcriptomic atlas of 15447 NB cells from eight NB samples,including paired samples of primary tumors and bone marrow *** used time-resolved analysis to chart the evolutionary trajectory of NB cells from the primary tumor to the metastases in the same patient and identified a common‘starter’subpopulation that initiates tumor development and ***‘starter’population exhibited high expression levels of multiple cell cycle-related genes,indicating the important role of cell cycle upregulation in NB tumor *** addition,our evolutionary trajectory analysis demonstrated the involvement of partial epithelial-to-mesenchymal transition(p-EMT)along the metastatic route from the primary site to the bone *** study provides insights into the program driving NB metastasis and presents a signature of metastasis-initiating cells as an independent prognostic indicator and potential therapeutic target to inhibit the initiation of NB metastasis.

关键词： single-cell RNA sequencing metastasis neuroblastoma epithelial-to-mesenchymal transition

Simulating androgen receptor selection in designer yeast

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2022年第4期7卷 1108-1116页

作者： Haoran Zhang Lu Zhang Yipeng Xu Shaoyong Chen zhenyi ma Mingdong Yao Fangyin Li Bo Li Yingjin Yuan Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin300072PR China School of Chemical Engineering and Technology Tianjin UniversityTianjin300072PR China Department of Urology The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)HangzhouChina Hematology-Oncology Division Department of MedicineBeth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMA02215USA School of Basic Medical Sciences Tianjin Medical UniversityTianjin300070PR China

Androgen receptor(AR)mutation is closely associated with prostate cancer(PCa)and is one of the mechanisms of resistance to PCa therapies such as AR *** sequencing technologies like next-generation sequencing(NGS)contributes to the high-throughput and precise detection of AR mutations carried by PCa patients,the lack of interpretations of these clinical genetic variants has still been a roadblock for PCa-targeted precision ***,we established a designer yeast reporter assay to simulate natural androgen receptor(AR)selection using AR *** HIS3 gene transactivation was associated with the ligand-induced recruitment of steroid receptor coactivator-1(SRC-1)by AR mutants,where yeast growth in histidine-free medium was determined as the *** assay is applicable to determine a wide range of clinical AR mutants including those with loss of function relating to androgen insensitivity syndrome(AIS),and those associated with PCa conferring resistance to AR antagonists such as enzalutamide(ENZ),bicalutamide(BIC),and cyproterone acetate(CPA).One clinical AR mutant previously reported to confer ENZ-resistance,F877L,was found to confer partial resistance to CPA as well using designer *** simple and efficient assay can enable precise one-pot screening of AR mutants,providing a reference for tailored medicine.

关键词： Androgen receptor mutations Androgen receptor antagonists Prostate cancer Saccharomyces cerevisiae

Lymphocyte lineage-restricted transcription factor Spi-B induces aniokis resistance by elevated autophagic process in lung cancer cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lymphocyte lineage-restricted transcription factor Spi-B ind...

Negative feedback loop between p66Shc and ZEB1 regulates fibrotic EMT response in lung cancer cells

第十二届全国免疫学学术大会

作者： Zhang Hua Liu Zhe ma zhenyi Department of Immunology Biochemistry and Molecular BiologyCollaborative Innovation Center of Tianjin for Medical EpigeneticsTianjin Key Laboratory of Medical EpigeneticsTianjin Medical University

Anoikis,detachment-induced cell death,is a form of programmed cell death induced by anchorage-dependent cells detaching from an extracellular ***,gain of anoikis resistance is an essential step for cancer metastasis to ***,we found that lymphocyte lineage-restricted transcription factor Spi-B,a member of the Ets family of transcription factors,is overexpressed in lung cancer cell lines that have been maintained to grow in the absence of extracellular *** of Spi-B in parental cells results in decreased anoikis resistance following cell detachment from *** also found that Spi-B induces anoikis resistance by promoting the macroautophagic process and therefore cell ***-B depletion by shRNA results in decreased ability of lung cancer cells to form tumors in *** data suggest that Spi-B as a pro-metastasis gene whose function is associated with anoikis resistance through elevated autophagic process in lung cancer cells.

关键词： Autophagy Transcription factor Spi-B Anoikis Lung cancer

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Negative feedback loop between p66Shc and ZEB1 regulates fib...

河南省桧树亭矿15采区注浆时冒浆防治技术研究

第十一届全国免疫学学术大会

作者： Li Xichuan Liu Zhe ma zhenyi Department of Immunology Tianjin Medical University

The epithelial-to-mesenchymal transition(EMT) program is crucial for the epithelial cancer progression and fibrotic diseases. Our previous work has demonstrated that p66 Shc, a focal adhesion-associated adaptor protein, is frequently downregulated in lung cancers and its depletion promotes metastasis behavior through anoikis resistance. However, mechanism underlying loss of p66 Shc and EMT response is not fully understood. Here, we showed that p66 Shc deficiency enhanced the expression of ZEB1, the known mesenchymal transcription factor and consequently increased Vimentin, and decreased epithelial markers of E-cadherin and β-catenin. p66 Shc depletion also increased cell invasion and migration. In addition, Ch IP and luciferase assays showed that these effects were directly mediated by ZEB1 repression of p66 Shc promoter. Thus, our findings define a critical role of p66 Shc in the suppression of fibrotic EMT response with a negative feedback loop between p66 Shc and ZEB1 in lung epithelial cancer cells.

关键词： EMT p66Shc Zeb1 Lung Cancer

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国煤炭地质 2022年第S1期34卷 82-85,151页

作者：马震仪中国煤炭地质总局水文地质局第三水文地质队河北邯郸056006

桧树亭矿主采煤层为二_(1)煤,井田构造发育复杂,治理区域内存在主要断层有郭庄断层(DF_(22))、油坊沟断层(DF_(17))及滑F_(1)断层,其中滑F_(1)断层为一低倾角滑动断层,导致煤层与奥灰顶界面之间部分地层缺失,隔水层厚度不均一。对15采... 详细信息

桧树亭矿主采煤层为二_(1)煤,井田构造发育复杂,治理区域内存在主要断层有郭庄断层(DF_(22))、油坊沟断层(DF_(17))及滑F_(1)断层,其中滑F_(1)断层为一低倾角滑动断层,导致煤层与奥灰顶界面之间部分地层缺失,隔水层厚度不均一。对15采区区域治理注浆情况进行了分析,表明DF_(17)断层为该区域内一主要导水通道,同时在DF_(17)断层附近注浆时发生多次地面及井下冒浆。通过对冒浆原因分析,发现其主要是由断层导通至地面所引起,且DF_(17)断层与滑F_(1)断层,在施加外力条件下,会发生联通。通过调整注浆工艺对断层通道进行治理加固,成功解决了冒浆问题,也为后续治理工程提供了方法依据,指明了重点防治方向。

关键词：桧树亭矿区域治理 DF_(17)断层滑F_(1)断层注浆冒浆

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

峰峰地区薄层灰岩定向水平分支构造探查实践

煤炭与化工 2019年第12期42卷 17-20页

作者：马震仪中国煤炭地质总局第三水文地质队河北邯郸056001

峰峰地区采用定向水平井技术对煤层底板含水层进行探查治理,其中构造导水为引发煤层底板奥灰承压水突水的最主要威胁。薄层(大青)灰岩作为煤底板距离奥灰较近的含水层,常作为奥灰水上部警示层,并且薄层灰岩水平井钻遇构造时,地层变化明... 详细信息

峰峰地区采用定向水平井技术对煤层底板含水层进行探查治理,其中构造导水为引发煤层底板奥灰承压水突水的最主要威胁。薄层(大青)灰岩作为煤底板距离奥灰较近的含水层,常作为奥灰水上部警示层,并且薄层灰岩水平井钻遇构造时,地层变化明显,在探查构造方面对比奥灰更具优势,以羊东矿8467工作面水平井为例,介绍了大青灰岩水平井探查构造情况,为峰峰地区类似水平井作以借鉴。

关键词：薄层灰岩水平井构造探查安全评价