检索结果-南通市图书馆

Glycodiversification of gentamicins through in vivo glycosyltransferase swapping enabled the creation of novel hybrid aminoglycoside antibiotics with potent activity and low ototoxicity

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第9期14卷 4149-4163页

作者： Xinyun Jian Cheng Wang Shijuan Wu Guo Sun Chuan Huang Chengbing Qiu Yuanzheng liu Peter FLeadlay Dong liu Zixin Deng Fuling Zhou Yuhui Sun Department of Hematology Zhongnan Hospital of Wuhan UniversitySchool of Pharmaceutical SciencesWuhan UniversityWuhan 430071China Key Laboratory of Combinatorial Biosynthesis and Drug Discovery(Ministry of Education) Wuhan UniversityWuhan 430071China School of Pharmacy Huazhong University of Science and TechnologyWuhan 430030China School of Life Sciences Co-Innovation Center of NeuroregenerationNantong Laboratory of Development and DiseasesNantong UniversityNantong 226019China Department of Biochemistry University of CambridgeCambridge CB21GAUK Department of Biochemistry and Molecular Biology Biomedicine Discovery InstituteMonash UniversityClayton VIC 3800Australia ARC Centre of Excellence for Innovations in Protein and Peptide Science Monash UniversityClayton VIC 3800Australia

Aminoglycosides(AGs)are a class of antibiotics with a broad spectrum of ***,their use is limited by safety concerns associated with nephrotoxicity and ototoxicity,as well as drug *** address these issues,semi-synthetic approaches for modifying natural AGs have generated new generations of AGs,however,with limited types of modification due to significant challenges in *** study explores a novel approach that harness the bacterial biosynthetic machinery of gentamicins and kanamycins to create hybrid *** was achieved by glycodiversification of gentamicins via swapping the glycosyltransferase(GT)in their producer with the GT from kanamycins biosynthetic pathway and resulted in the creation of a series of novel AGs,therefore referred to as genkamicins(GKs).The manipulation of the hybrid biosynthetic pathway enabled the targeted accumulation of different GK species and the isolation and characterization of six GK *** compounds display retained antimicrobial activity against a panel of World Health Organization(WHO)critical priority pathogens,and GK-C2a,in particular,demonstrates low ototoxicity compared to clinical drugs in zebrafish *** study provides a new strategy for diversifying the structure of AGs and a potential avenue for developing less toxic AG drugs to combat infectious diseases.

关键词： Antibiotic Aminoglycoside biosynthesis Biosynthetic engineering Ototoxicity

Scene text recognition via dual character counting-aware visual and semantic modeling network

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2024年第3期67卷 313-314页

作者： Ke XIAO Anna ZHU Brian Kenji IWANA Cheng-Lin liu School of Computer Science and Artificial Intelligence Wuhan University of Technology Human Interface Laboratory Kyushu University State Key Laboratory of Multimodal Artificial Intelligence Systems Institute of AutomationChinese Academy of Sciences School of Artificial Intelligence University of Chinese Academy of Sciences

Scene text recognition(STR) is drawing increasing attention nowadays due to its wide application in real life. Character counting information, as auxiliary information, has been shown to be effective in boosting text ... 详细信息

关键词： Decoding

Whose Aid is Beneficial to Firms'Exports?Evidence from a Post-disaster Aid Experiment in China

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

China & World Economy 2024年第2期32卷 73-96页

作者： Tan Li Qing liu Lihe Xu School of International Business Southwestern University of Finance and EconomicsChina School of Economics Renmin University of ChinaChina Institute of Studies for the Greater Bay Area Guangdong University of Foreign StudiesChina

Post-disaster aid is widely regarded as important in helping local recovery and *** paper examines the effectiveness of post-disaster aid on exports,which are a driving factor of economic *** reports a natural experiment in China–the case of post-disaster aid following the Wenchuan earthquake in 2008–to examine how donors'experiences affected the exports of manufacturing firms in disaster-stricken *** export experience of the donor was *** coming from donors with more export experience was more beneficial to the exports of firms in recipient counties than aid from less experienced donors.“Learning from the donor”is a potential channel through which this effect *** is,firms in recipient counties learned from donors'export experience by exporting more products similar to those of donors,exporting more to the destination countries of donors,and participating in the donors'supply-chain networks by exporting more of the donors'***“learning from the donor”effects show that knowledge spillover can occur between spatially distant parties,which complements the literature.

关键词： China export knowledge spillover post-disaster aid

A polarization sensitive interferometer:Delta interferometer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics B 2024年第3期33卷 369-377页

作者：卫超奇刘建彬董翼飞孙雨农周宇郑淮斌刘严严闫秀生李福利徐卓 Key Laboratory of Multifunctional Materials and Structures Ministry of Education & International Center for Dielectric ResearchSchool of Electronic Science and EngineeringXi'an Jiaotong UniversityXi'an 710049China MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter Department of Applied PhysicsXi'an Jiaotong UniversityXi'an 710049China Science and Technology on Electro-Optical Information Security Control Laboratory Tianjin 300308China

A new type of polarization sensitive interferometer is proposed,named the Delta interferometer,inspired by its geometry resembling the Greek letter *** main difference between the Delta interferometer and other existing interferometers,such as Michelson,Mach-Zehnder and Young's double-slit interferometers,is that the two interfering paths are asymmetrical in the Delta *** visibility of the first-order interference pattern observed in the Delta interferometer is dependent on the polarization of the incidental *** coherence theory is employed to interpret this phenomenon and single-mode continuous-wave laser light is employed to verify the theoretical *** theoretical and experimental results are *** Delta interferometer is a perfect tool to study the reflection of electromagnetic fields in different polarizations and may find applications in polarization-sensitive scenarios.

关键词： phase sensitive interferometer Fresnel formula first-order interference

在线全文

学校读者我要写书评

暂无评论

淮北临涣矿采煤沉陷区生态系统健康评价

四川环境 2021年第3期40卷 164-171页

作者：穆红波任梦溪陈永春郑刘根安士凯谢毫煤矿生态环境保护国家工程实验室安徽淮南232001 安徽大学资源与环境学院安徽省矿山生态修复工程实验室合肥230601

根据淮北临涣采煤沉陷区水生态调查数据,基于方差赋权的生态系统综合指数法,采用主成分分析与相关性分析方法进行指标筛选,建立了沉陷区生态系统健康评价指标体系。结果表明:(1)功能区周边环境大致相同,沉陷区西部废水排放区环境最差,... 详细信息

根据淮北临涣采煤沉陷区水生态调查数据,基于方差赋权的生态系统综合指数法,采用主成分分析与相关性分析方法进行指标筛选,建立了沉陷区生态系统健康评价指标体系。结果表明:(1)功能区周边环境大致相同,沉陷区西部废水排放区环境最差,东部近矸石山区状况最好,其综合健康指数值分别为0.422、0.566;(2)临涣采煤沉陷区生态系统处于亚健康状态,栖息地环境和水生生物指标分别为0.337 5和0.256 6,是影响临涣采煤沉陷水域生态系统最大的两个指标类型。矿业生产活动、露天矿业废弃物堆放、农业生产和居民生活污水是导致临涣采煤沉陷区生态系统亚健康的主要原因。

关键词：采煤沉陷区生态系统综合指数法健康评价

Revisiting the spin effects induced by thermal vorticity

在线全文

学校读者我要写书评

暂无评论

Chinese Physics C 2024年第5期48卷 194-208页

作者：高建华杨诗正 Shandong Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment School of Space Science and PhysicsShandong UniversityWeihai 264209China Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation(MOE)Shandong UniversityQingdao 266237China

We revisit the spin effects induced by thermal vorticity by calculating them directly from the spin-dependent distribution *** spin-1/2 particles,we provide the polarization up to the first order of thermal vorticity and compare it with the usual results calculated from the spin *** spin-1 particles,we show that all the non-diagonal elements vanish and there is no spin alignment up to the first order of thermal *** present the spin alignment at second-order contribution from thermal *** also show that the spin effects for both Dirac and vector particles receive an extra contribution when the spin direction is associated with the momentum of the particle.

关键词： relativistic heavy ion collisions global polarization quark gluon plasma

Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第2期23卷 724-730页

作者： Wei Huang Ruyu Jiao Hongtao Cheng Shengli Cai Jia liu Qiong Hu Lili liu Bao Li Tonghua Wang Mei Li Dawei Zhang Mingli Yan Hunan Research Center of Heterosis Utilization in Rapeseed/Crop Research Institute Hunan Academy of Agricultural SciencesChangsha 410125China Hunan Key Laboratory of Economic Crops genetic Improvement and Integrated Utilization/School of Life and Health Science Hunan University of Science and TechnologyXiangtan 411201China Key Laboratory of Biology and genetic Improvement of Oil Crops Ministry of Agriculture and Rural Affairs/Oil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062China

The yellow seed trait is preferred by breeders for its potential to improve the seed quality and commercial value of Brassica *** the present study,we produced yellow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homologs were knocked *** staining of the seed coat demonstrated that proanthocyanidin accumulation was significantly reduced in the pap2 double mutants and decreased specifically in the endothelial and palisade layer cells of the seed *** and metabolite profiling analysis suggested that disruption of the BnPAP2 genes could reduce the expression of structural and regulated genes in the phenylpropanoid and flavonoid biosynthetic *** broad suppression of these genes might hinder proanthocyanidin accumulation during seed development,and thereby causing the yellow seed trait in *** results indicate that BnPAP2 might play a vital role in the regulatory network controlling proanthocyanidin accumulation.

关键词： yellow seed BnPAP2 proanthocyanidins CRISPR/Cas9

Targeting both ferroptosis and pyroptosis may represent potential therapies for acute liver failure

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第33期30卷 3791-3798页

作者： Zhong-Yuan Xing Chuan-Jie Zhang Li-Juan liu Department of Medical Microbiology School of Basic Medical SciencesWuhan UniversityWuhan 430071Hubei ProvinceChina Department of Children Health Care Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430061Hubei ProvinceChina

In this editorial,we comment on the article published in the recent issue of the World Journal of *** liver failure(ALF)is a fatal disease that causes uncontrolled massive hepatocyte death and rapid loss of liver *** and pyroptosis,cell death forms that can be initiated or blocked concurrently,can play significant roles in developing inflammation and various ***,their roles in ALF remain *** article discovered the positive feedback between ferroptosis and pyroptosis in the progression of ALF,and revealed that the silent information regulator sirtuin 1(SIRT1)inhibits both pathways through p53,dramatically reducing inflammation and protecting *** suggests the potential use of SIRT1 and its downstream molecules as therapeutics for ***,we will discuss the role of ferroptosis and pyroptosis in ALF and the crosstalk between these cell death ***,we address potential treatments that could alleviate ALF by simultaneously inhibiting both cell death pathways,as well as examples of SIRT1 activators being used as disease treatment strategies,providing new insights into the therapy of ALF.

关键词： Acute liver failure Ferroptosis Pyroptosis Crosstalk Silent information regulator sirtuin 1 P53 Glutathione peroxidase 4 Gasdermin D Treatment

Structures and ion transport mechanisms of plant high-affinity potassium transporters

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2024年第3期17卷 409-422页

作者： Jiangqin Wang Yanping Luo Fan Ye Zhong Jie Ding Shao Jian zheng Shuai Qiao Yong Wang Jiangtao Guo Wei Yang Nannan Su international Institutes of Medicine the Fourth Affiliated HospitalZhejang University School of MedicineYiwuZhejiang 322000China Department of Biophysics and Department of Neurology of the Fourth Affiliated Hospital Zhejiang University School of MedicineHangzhouZhejang 310058China State Key Laboratory of Plant Environmental Resilience College of Life SciencesZhejang UniversityHangzhouZhejang 310058China College of Life Sciences Zhejiang UniversityHangzhouZhejiang 310058China NHC and CAMS Key Laboratory of Medical Neurobiology MOE Frontier Science Center for Brain Science and Brain-machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhouZhejiang 310058China Nanhu Brain-computer Interface Institute Hangzhou.Zheiang 311100.China

Plant high-affinity K^(+) transporters(HKTs)mediate Na^(+) and K^(+) uptake,maintain Na^(+)/K^(+) homeostasis,and therefore play crucial roles in plant salt *** this study,we present cryoelectron microscopy structures of HKTs from two classes,classI HKT1;1 from Arabidopsis thaliana(AtHKT1;1)and class II HKT2;1 from Triticum aestivum(TaHKT2;1),in both Na^(+) -and K^(+) -bound states at 2.6-to 3.0-A ***1;1and TaHKT2;1function *** HKT subunit consists of four tan-dem domain units(D1-D4)with a repeated K^(+) -channel-like M-P-M *** each subunit,D1-D4 assemble into an ion conduction pore with a pseudo-four-fold *** both TaHKT2;1and AtHKT1;1 have only one putative Na^(+) ion bound in the selectivity filter with a similar coordination pattern,the two HKTs display different K^(+) binding modes in the ***2;1 has three K^(+) ions bound in the selec-tivity filter,but AtHKT1;1 has only two K^(+) ions bound in the filter,which has a narrowed external entrance due to the presence of a Ser residue in the first filter *** structures,along with compu-tational,mutational,and electrophysiological analyses,enable us to pinpoint key residues that are critical for the ion selectivity of *** findings provide new insights into the ion selectivity and ion transport mechanisms of plant HKTs and improve our understanding about how HKTs mediate plant salt tolerance and enhance crop growth.

关键词： HKTs structure ion selectivity mechanism salt tolerance