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Identification and Characterization of Nuclear Localization Signals within the Nucleocapsid Protein VP15 of White Spot Syndrome Virus

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Virologica Sinica 2009年第1期24卷 71-76页

作者： Li-juan LI Hua-jun ZHANG Cong ZHANG zheng-li shi State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China College of Fishery Huazhong Agricultural University Wuhan 430070 China

The nucleocapsid protein VP15 of white spot syndrome virus (WSSV) is a basic DNA-binding protein. Three canonical bipartite nuclear localization signals (NLSs), called NLS1 (aa 11-27), NLS2 (aa 33-49) and NLS3 (44-60), have been detected in this protein, using the ScanProsite computer program. To determine the nuclear localization sequence of VP15, the full-length open reading frame, or the sequence of one of the three NLSs, was fused to the green fluorescent protein (GFP) gene, and transiently expressed in insect Sf9 cells. Transfection with full-length VP15 resulted in GFP fluorescence being distributed exclusively in the nucleus. NLS1 alone could also direct GFP to the nucleus, but less efficiently. Neither of the other two NLSs (NLS2 and 3) was functional when expressed alone, but exhibited similar activity to NLS1 when they were expressed as a fusion peptide. Furthermore, a mutated VP15, in which the two basic amino acids (11RR12) of NLS1 were changed to two alanines (11AA12), caused GFP to be localized only in the cytoplasm of Sf9 cells. These results demonstrated that VP15, as a nuclear localization protein, needs cooperation between its three NLSs, and that the two residues (11RR12) of NLS1 play a key role in transporting the protein to the nucleus.

关键词： White spot syndrome virus (WSSV) Nucleocapsid protein, VP15 Nuclear localization signal (NLS)

Quantitative Trait Loci for Resistance Against Fusarium Wilt Based on Three Cotton F_2 Populations

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Agricultural Sciences in China 2010年第12期9卷 1799-1806页

作者： WANG Pei-zheng shi Li-fang SU Li HU Bao-min Xinjiang Condy Seed Science ＆ Technology Development Co. Ltd Urumqi 830011 P.R.China

Fusarium wilt （FW） is one of the most common cotton diseases in the world. Identification of QTLs conferring resistance to FW is key for the incorporation of resistance genes into elite cultivars. Two intraspecific （cross between Gossypium hirsuturn L.） and one interspecific （cross between Gossypium hirsutum L. and Gossypium bardence L.） F2 populations were constructed by using a highly resistant cultivar and crossing it to a susceptible cultivar with 154, 79, and 148 offsprings, respectively. Simple sequence repeats （SSR） were used to screen genomic regions closely linked to FW resistance. The results showed that five QTLs associated with FW resistance were detected in two intraspecific populations using a composite interval mapping method under four different conditions. Four of these loci located on Chr. 2/Chr. 17 neighboring markers JESPR304 or CIR305 which explained 13.1 to 45.9% of the phenotypic effect. Furthermore, JESPR304 and CIR305 were previously testified and found to be tightly linked. It is possible that these four QTLs detected under different conditions were the same resistance QTL/gene. We consider that there is the possibility of a major FW resistant gene in intraspecific populations. In the interspecific mapping populations two QTLs were detected on Chr. 9 and Chr. 12/26 which explained great phenotypic variance of 49.4 and 45.7%. As the location of QTLs for FW resistance among the intraspecific and the interspecfic populations were totally different, it is suggested that there may be different resistance mechanisms between G. bardence L. and G. hursutum L. Thus, the present research provides an opportunity to understand the genetic control of resistance to FW in Gossypium hirsutum and Gossypium bardence and to conduct MAS in breeding programs to develop FW resistant cultivars.

关键词： cotton Fusarium wilt resistance QTL molecular mapping

Recent Progress in Fabrication and Structural Design of Thermal Conductive Polymer Composites

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Chinese Journal of Polymer Science 2024年第3期42卷 277-291,I0004页

作者： Yuan-Kai Du zheng-Xue shi Shan Dong Hui Jin Xue Ke Pei Zhao Bing-Bing Jiang Feng You Hubei Key Laboratory of Plasma Chemistry and Advanced Materials School of Material Science and EngineeringWuhan Institute of TechnologyWuhan 430205China Key Laboratory of Green Preparation and Application of Functional Materials Ministry of EducationHubei UniversityWuhan 430062China Institute of Molecular Aggregation Science Tianjin UniversityTianjin 300072China

In recent years, the demand direction for electronic equipment has expanded into embedded and miniaturized devices. The heat radiation problem has become one of the most significant factors for hindering the development of electronic devices. Since heat radiation material is one of the important components in electronic devices, the demand for enhancing thermal conductivity is also increasingly urgent. Research on thermal conductive polymer composites has become a major direction for developing functional composites. This work reviewed the recent progress in the fabrication of thermal conductive polymer composites. Five different structures are presented, including the using of single fillers,hybrid fillers, double threshold percolation structure, segregated structure and other complex multiphase structures. Specifically, the preparation of high-performance thermal conductive polymer composites was introduced through the combination of various thermal conductive ***, the development direction of high thermal conductive polymer composites was briefly explored.

关键词： Thermal conductive polymer Hybrid fillers Polymer composite Thermal resistance

Effects of Monoclonal Antibody Against Porcine 40-kDa Adipocyte-Specific Membrane Protein on Endocrine Secretion in Pigs

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Agricultural Sciences in China 2009年第8期8卷 1004-1009页

作者： LIU Ling-yun HU Hong-mei ZHAO Su-mei ZHANG Xi DUAN Gang GAO shi-zheng Kunming Normal School Kunming 650201 P.R.China Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences Jinan 250100 P.R.China Yunnan Key' Laboratory of Animal Nutrition attd Feed Yunnan Agricultural University Kunming 650201 P..R. China

The present study was to investigate the effect of monoclonal antibody against porcine 40-kDa adipocyte-specific membrane protein on endocrine secretion in pigs, in order to provide the evidence for application of this antibody to reduce excessive fat deposition in pig production. 40 Landrace×Saba pigs were randomly divided into 8 groups： 2 control groups were given saline with 10 mL, respectively, and the 6 treatment groups were given monoclonal antibody against porcine 40-kDa adipocyte-specific membrane protein with 0.1, 0.5, and 1.0 mg ·kg^-1 body weight at 15 or 60 kg body weight, respectively, all treatments were performed by intraperitoneal injection. The results showed that this monoclonal antibody could significantly reduce serum insulin level and increase levels of serum growth hormone （GH）, insulin-like growth factor-1（IGF-1）, triiodothyronine （T3）, and tetraiodothyronine （T4） either at 15 or 60 kg body weight injection. However, more marked effect was observed at 15 kg body weight treatment. Moreover, the dose-dependent effect of this monoclonal antibody on endocrine secretion was also observed. This result revealed that this monoclonal antibody increased secretion of hormones regulating fat lysis and reduced secretion of hormones regulating fat synthesis, suggests the reduction of porcine excessive fat deposition by this monoclonal antibody was carried out through affecting hormones regulating fat metabolism.

关键词： adipocyte membrane protein monoclonal antibody endocrine pig

Multiscale classification and its application to process monitoring

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第6期11卷 425-434页

作者： Yu-ming LIU Lu-bin YE Ping-you zheng Xiang-rong shi Bin HU Jun LIANG Institute of Industrial Control State Key Lab of Industrial Control Technology Zhejiang University Hangzhou 310027 China

Multiscale classification has potential advantages for monitoring industrial processes generally driven by events in different time and frequency *** this study, we adopt stationary wavelet transform for multiscale analysis and propose an applicable scale selection method to obtain the most discriminative scale *** using the multiscale features, we construct two classifiers:(1) a supported vector machine(SVM) classifier based on classification distance, and(2) a Bayes classifier based on probability *** the SVM classifier, we use 4-fold cross-validation and grid-search to obtain the optimal *** the Bayes classifier, we introduce dimension reduction techniques including kernel Fisher discriminant analysis(KFDA) and principal component analysis(PCA) to investigate their influence on classification *** tested the classifiers with two simulated benchmark processes:the continuous stirred tank reactor(CSTR) process and the Tennessee Eastman(TE) *** also tested them on a real polypropylene production *** performance comparison among the classifiers in different scales and scale combinations showed that when datasets present typical scale features, the multiscale classifier had higher classification accuracy than conventional single scale *** also found that dimension reduction can generally contribute to a better classification in our tests.

关键词： Multiscale analysis Stationary wavelet transform Multi-class classifier Feature extraction Process monitoring

Urotensin Ⅱ-induced insulin resistance is mediated by NADPH oxidase-derived reactive oxygen species in Hep G2 cells

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World Journal of Gastroenterology 2016年第25期22卷 5769-5779页

作者： Ying-Ying Li zheng-Ming shi Xiao-Yong Yu Ping Feng Xue-Jiang Wang Department of Physiology and Pathophysiology School of Basic Medical Sciences Capital Medical University Beijing 100069 China Beijing Key Laboratory for Cancer Invasion and Metastasis Research Capital Medical University Beijing 100069 China Department of General Surgery Beijing Jishuitan Hospital Beijing 100069 China

AIM: To investigated the effects of urotensin Ⅱ(UII) on hepatic insulin resistance in Hep G2 cells and the potential mechanisms ***: Human hepatoma Hep G2 cells were cultured with or without exogenous UII for 24 h, in the presence or absence of 100 nmol/L insulin for the last 30 min. Glucose levels were detected by the glucoseoxidase method and glycogen synthesis was analyzed by glycogen colorimetric/fluorometric assay. Reactive oxygen species(ROS) levels were detected with a multimode reader using a 2′,7′-dichlorofluorescein diacetate probe. The protein expression and phosphorylation levels of c-Jun N-terminal kinase(JNK), insulin signal essential molecules such as insulin receptor substrate-1(IRS-1), protein kinase B(Akt), glycogen synthase kinase-3β(GSK-3β), and glucose transporter-2(Glut 2), and NADPH oxidase subunits such as gp91 phox, p67 phox, p47 phox, p40 phox, and p22 phox were evaluated by Western ***: Exposure to 100 nmol/L UII reduced the insulin-induced glucose consumption(P < 0.05)and glycogen content(P < 0.01) in Hep G2 cells compared with cells without UII. UII also abolished insulin-stimulated protein expression(P < 0.01) and phosphorylation of IRS-1(P < 0.05), associated with down-regulation of Akt(P < 0.05) and GSK-3β(P < 0.05) phosphorylation levels, and the expression of Glut 2(P < 0.001), indicating an insulin-resistance state in Hep G2 cells. Furthermore, UII enhanced the phosphorylation of JNK(P < 0.05), while the activity of JNK, insulin signaling, such as total protein of IRS-1(P < 0.001), phosphorylation of IRS-1(P < 0.001) and GSK-3β(P < 0.05), and glycogen synthesis(P < 0.001) could be reversed by pretreatment with the JNK inhibitor SP600125. Besides, UII markedly improved ROS generation(P < 0.05) and NADPH oxidase subunit expression(P < 0.05). However, the antioxidant/NADPH oxidase inhibitor apocynin could decrease UII-induced ROS production(P < 0.05), JNK phosphorylation(P < 0.05), and insulin resistance(P < 0.05

关键词： Urotensin Ⅱ Insulin resistance NADPH oxidase Reactive oxygen species HepG 2 cells

Inverse analysis of coupled carbon-nitrogen cycles against multiple datasets at ambient and elevated CO_(2)

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Journal of Plant Ecology 2016年第3期9卷 285-295页

作者： zheng shi Yuanhe Yang Xuhui Zhou Ensheng Weng Adrien C.Finzi Yiqi Luo Department of Botany and Microbiology University of OklahomaNormanOK 73019USA State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China Department of Biology Boston UniversityBostonMA 02215USA

Aims Carbon(C)sequestration in terrestrial ecosystems is strongly regulated by nitrogen(N)***,key parameters that determine the degree of N regulation on terrestrial C sequestration have not been well *** Here,we used a Bayesian probabilistic inversion approach to estimate 14 target parameters related to ecosystem C and N interactions from 19 datasets obtained from Duke Forests under ambient and elevated carbon dioxide(CO_(2)).Important FindingsOur results indicated that 8 of the 14 target parameters,such as C:N ratios in most ecosystem compartments,plant N uptake and external N input,were well constrained by available datasets whereas the others,such as N allocation coefficients,N loss and the initial value of mineral N pool were poorly *** analysis showed that elevated CO_(2)led to the increases in C:N ratios in foliage,fine roots and ***,elevated CO_(2)stimulated plant N uptake and increased ecosystem N capital in Duke Forests by 25.2 and 8.5%,*** addition,elevated CO_(2)resulted in the decrease of C exit rates(*** in C residence times)in foliage,woody biomass,structural litter and passive soil organic matter,but the increase of C exit rate in fine *** results demonstrated that CO_(2)enrichment substantially altered key parameters in determining terrestrial C and N interactions,which have profound implications for model improvement and predictions of future C sequestration in terrestrial ecosystems in response to global change.

关键词： Bayesian probabilistic inversion carbon-nitrogen interactions carbon-nitrogen coupled model Duke FACE.

Prevalence of Three Shrimp Viruses in Zhejiang Province in 2008

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Virologica Sinica 2011年第1期26卷 67-71页

作者： Xiao-wei Yu Jian-ping Wang Wei Zhang zheng-li shi Faculty of Life Science and Biotechnology Ningbo University Ningbo 315211China Ningbo Academy of Ocean and Fishery Ningbo 315012 China State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

White spot syndrome virus (WSSV),Taura syndrome virus (TSV) and Infectious hypodermal and haematopoietic necrosis virus (IHHNV) are three shrimp viruses responsible for major pandemics affecting the shrimp farming industry. Shrimps samples were collected from 12 farms in Zhejiang province,China,in 2008 and analyzed by PCR to determine the prevalence of these viruses. From the 12 sampling locations,8 farms were positive for WSSV,8 for IHHNV and 6 for both WSSV and IHHNV. An average percentage of 57.4% of shrimp individuals were infected with WSSV,while 49.2% were infected with IHHNV. A high prevalence of co-infection with WSSV and IHHNV among samples was detected from the following samples:Bingjiang (93.3%),liuao (66.7%),Jianshan (46.7%) and Xianxiang (46.7%). No samples exhibited evidence of infection with TSV in collected samples. This study provides comprehensive information of the prevalence of three shrimp viruses in Zhejiang and may be helpful for disease prevention control in this region.

关键词： Molecular detection White spot syndrome virus (WSSV) Taura syndrome virus (TSV) Infectious hypodermal and haematopoietic necrosis virus (IHHNV)

Elevation of the Power Factor of Co4Sb12 Skutterudite with Sm-Doping in High-Pressure High-Temperature Synthesis

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Chinese Physics Letters 2010年第6期27卷 237-239页

作者： JIANG Yi-Ping JIA Xiao-Peng GUO Wei XU Hui-Wen DENG Le zheng shi-Zhao MA Hong-An National Lab of Superhard Materials Jinlin University Changchun 130012 Department of Physics Beihua University Jilin 132013

A thermoelectric material Smx Co4Sb12 （0（ x ≤1.0） is synthesized at high pressure and high temperature leading to an enhanced power factor. The experimental measurements show that the SmxCo4Sb12 compounds exhibits n-type conduction. The absolute value of the Seebeck coefficient decreases with increasing Sm fraction. The resistivity increases with samarium content x from 0.1 to 0.2, but decreases dramatically when x changes from 0.2 to 1.0. The maximum power factor reaches 13.1 μ***^-1K-2 at x =1.0, which is larger than the data previously reported for the La-doped CoSb3 prepared at room pressure.

关键词： Pressure Energy sources Measurement