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Advances in CRISPR-based gene editing technology and its application in nucleic acid detection

BIOCELL 2025年第1期49卷 21-43页

作者： LIUJIE CHEN LILI DUAN JIA LI JUN CHEN DUANFANG LIAO NONGYUE HE KAI LI zheng hu Translational Medicine Institute the First People’s Hospital of ChenzhouHengyang Medical SchoolUniversity of South ChinaChenzhou423000China The First Clinical College of Xiangnan University The First Affiliated Hospital of Xiangnan UniversityChenzhou423000China National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology Hunan University of Chinese MedicineChangsha410208China State Key Laboratory of Digital Medical Engineering School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China

Nucleic acid analysis is a key technique that enables accurate detection of various *** nucleic acid testing typically requires access to specialized laboratories,equipment,and trained personnel,which hinders the widespread use of on-site testing for DNA and RNA ***,integrating gene editing technology with traditional nucleic acid detection methods,especially isothermal amplification technology,can help overcome the limitations associated with on-site *** combination can accomplish precise and swift detection of nucleic acid sequences,offering a robust tool for on-site *** Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins(CRISPR/Cas)technology,which comprises the CRISPR system and Cas effector proteins,is a powerful tool that is advancing the field of nucleic acid ***,Cas12,Cas13,and Cas14 proteins have emerged as straightforward,effective,precise,sensitive,and cost-effective methods for in vitro nucleic acid detection because of their“collateral cleavage”*** combined with the“collateral cleavage”ability of Cas protein and isothermal amplification,CRISPR/Cas systems have great potential to advance nucleic acid *** article summarizes the research progress of different CRISPR/Cas systems and their applications in nucleic acid detection and future perspectives.

关键词： CRISPR/Cas Pathogen Clinical application

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Correlation between gut microbiota and tumor immune microenvironment:A bibliometric and visualized study

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World Journal of Clinical Oncology 2025年第2期16卷 110-129页

作者： zheng-Jun hu hui-Rong Zhu Yong-Jie Jin Pan Liu Xiao-Wei Yu Yu-Ren Zhang Department of Oncology Shanghai Jiading District Hospital of Traditional Chinese MedicineShanghai 200000China Department of Oncology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200000China School of Chinese Medicine Anhui University of Traditional Chinese MedicineHefei 230000Anhui ProvinceChina

BACKGROUND In recent years,numerous reports have been published regarding the relationship between the gut microbiota and the tumor immune microenvironment(TIME).However,to date,no systematic study has been conducted on the relationship between gut microbiota and the TIME using bibliometric *** To describe the current global research status on the correlation between gut microbiota and the TIME,and to identify the most influential countries,research institutions,researchers,and research hotspots related to this *** We searched for all literature related to gut microbiota and TIME published from January 1,2014,to May 28,2024,in the Web of Science Core Collection *** then conducted a bibliometric analysis and created visual maps of the published literature on countries,institutions,authors,keywords,references,etc.,using CiteSpace(6.2R6),VOSviewer(1.6.20),and bibliometrics(based on R 4.3.2).RESULTS In total,491 documents were included,with a rapid increase in the number of publications starting in *** country with the highest number of publications was China,followed by the United *** has the highest number of citations in *** a centrality perspective,the United States has the highest influence in this *** institutions with the highest number of publications were Shanghai Jiao Tong University and Zhejiang ***,the institution with the most citations was the United States National Cancer *** authors,Professor Giorgio Trinchieri from the National Institutes of Health has the most local impact in this *** most cited author was Fan *** results of journal publications showed that the top three journals with the highest number of published papers were Frontiers in Immunology,Cancers,and Frontiers in *** three most frequently used keywords were gut microbiota,tumor microenvironment,and *** This study systematically elaborates on the research progress re

关键词： Gut microbiota Tumor immune microenvironment Bibliometric CiteSpace VOSviewer R-bibliometrics

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基于层次特征增强的细粒度点云分类

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浙江大学学报(理学版) 2025年第1期52卷 70-80页

作者：白静刘路郑虎蒋金哲北方民族大学计算机科学与工程学院宁夏银川750021 国家民委图像图形智能处理实验室宁夏银川750021

针对粗粒度点云分类方法在细粒度数据集中局部特征提取不足的问题,提出了一种基于层次特征增强的三维细粒度点云分类网络(HFE-Net)。基于Veronese映射的点特征增强模块(V-PE)对点云数据进行数据增强,辅助网络学习法线和姿态高阶信息;基... 详细信息

针对粗粒度点云分类方法在细粒度数据集中局部特征提取不足的问题,提出了一种基于层次特征增强的三维细粒度点云分类网络(HFE-Net)。基于Veronese映射的点特征增强模块(V-PE)对点云数据进行数据增强,辅助网络学习法线和姿态高阶信息;基于多尺度上下文感知的簇内特征增强模块(CA-IntraCE),利用不同尺度的K近邻(K-nearest neighbors,KNN)算法以及交叉注意力实现不同尺度特征的增强,以消除最大池化带来的信息丢失;基于分组稀疏采样的簇间特征增强模块(GSS-InterCE),利用最远点采样(FPS)算法获得稀疏点,并采用交叉注意力实验不同簇间的特征增强,从而提高网络的细粒度判别能力。在FG3D数据集Airplane、Car和Chair 3个类别上的实验结果显示,HFE-Net的总体准确率分别达97.40%,80.53%和83.83%,已超过现有最优方法DC-Net、FGPNet的分类框架,说明HFE-Net的分类性能具有一定的优越性。

关键词：三维点云细粒度分类交叉注意力特征增强

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全氟辛烷磺酸新型替代物6:2氟调聚磺酸/盐的环境检测及毒性研究进展

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环境化学 2025年第1期44卷 28-40页

作者：陶秋英胡正王献中南民族大学化学与材料科学学院武汉430074

全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)是全氟和多氟烷基化合物(per-and polyfluoroalkyl substances,PFASs)的代表物质,因其生物累积性、环境持久性以及潜在的致癌风险,于2009年被列入《斯德哥尔摩公约》的管控名单.随着PFOS... 详细信息

全氟辛烷磺酸(perfluorooctane sulfonate,PFOS)是全氟和多氟烷基化合物(per-and polyfluoroalkyl substances,PFASs)的代表物质,因其生物累积性、环境持久性以及潜在的致癌风险,于2009年被列入《斯德哥尔摩公约》的管控名单.随着PFOS的生产和使用受到国际社会的严格限制,6:2氟调聚磺酸/盐(6:2 fluorotelomer sulfonic acid/sulfonate,6:2 FTSA)作为其替代品,在全球范围内分布日益广泛,对生态环境构成了新的威胁,被认为是一种潜在的新型污染物.尽管如此,目前对6:2 FTSA的研究仍处于起步阶段,相关科学知识体系亟待完善.本文系统性地综述了6:2 FTSA的检测技术、环境分布、生物毒性及其作用机制,以及转化与降解方面的最新研究进展.重点分析了6:2 FTSA的检测方法、生物毒性机制研究的现状,以及降解工艺的开发与优化.通过对这些关键问题的剖析,揭示了当前研究领域面临的挑战,并展望了未来可能的研究方向,为评估6:2 FTSA作为PFOS替代品的合理性提供理论基础与实验依据.

关键词：新型污染物 6:2 氟调聚磺酸/盐环境分布生物毒性转化与降解.

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有机肥代替氮肥对中国主要粮食产量的影响

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环境科学 2025年第1期46卷 478-488页

作者：朱银浩尹娟马正虎杨莹攀宁夏大学土木与水利工程学院银川750021 旱区现代农业水资源高效利用教育部工程中心银川750021 宁夏节水灌溉与水资源调控工程技术研究中心银川750021

为了综合评估有机肥替代氮肥对中国主要粮食产量的变化规律,以102篇文献为研究对象,通过Meta分析定量探讨了土壤理化性质、气候条件及不同氮肥替代率和补施量的影响.结果表明,与单施氮肥相比,有机肥替代氮肥可以提高作物产量,土壤速效... 详细信息

为了综合评估有机肥替代氮肥对中国主要粮食产量的变化规律,以102篇文献为研究对象,通过Meta分析定量探讨了土壤理化性质、气候条件及不同氮肥替代率和补施量的影响.结果表明,与单施氮肥相比,有机肥替代氮肥可以提高作物产量,土壤速效钾含量、pH值、年平均气温对3种主要粮食产量影响最显著,当氮肥替代率和补施氮肥量分别为31.06%和241.78kg·hm^(-2)、26.52%和173.31 kg·hm^(-2)、39.23%和149.17 kg·hm^(-2)时,玉米、小麦和水稻产量增幅比达到最优,分别为3.31%、3.32%和1.08%.研究结果可为今后作物生产合理施肥提供参考依据,有助于促进绿色农业发展.

关键词：氮肥有机肥 Meta分析产量中国

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变泊松比蜂窝夹芯结构的吸波性能优化设计

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复合材料科学与工程 2025年第1期 67-75页

作者：孙士平常梦源胡政南昌航空大学航空制造工程学院南昌330063

采用泊松比分类六边形蜂窝构型,基于均匀化理论建立蜂窝单胞参数化有限元模型,开展了变泊松比蜂窝夹芯结构的吸波性能分析与给定反射率目标的蜂窝构型优化。计算结果表明:2~18 GHz电磁波垂直入射工况下,减小蜂窝边长和增大蜂窝高度均能... 详细信息

采用泊松比分类六边形蜂窝构型,基于均匀化理论建立蜂窝单胞参数化有限元模型,开展了变泊松比蜂窝夹芯结构的吸波性能分析与给定反射率目标的蜂窝构型优化。计算结果表明:2~18 GHz电磁波垂直入射工况下,减小蜂窝边长和增大蜂窝高度均能有效提高结构吸波性能,使泊松比趋于零的蜂窝角度变化可提高吸波性能,存在与蜂窝几何参数匹配的最佳涂层厚度,能够获得最优吸波性能;与正泊松比常规六边形蜂窝、零泊松比半内凹六边形蜂窝和负泊松比内凹六边形蜂窝的初始方案相比,反射率为-10 dB时,三种优化蜂窝吸收带宽分别增大了56.8%、35.2%、52.5%,而反射率为-20 dB时,三种优化蜂窝吸收带宽分别从零增大为4 GHz、2.3 GHz、0.5 GHz;三种泊松比蜂窝中,正泊松比蜂窝的优化适应潜力最好。研究结果为多功能蜂窝吸波结构的蜂窝构型设计提供了参考。

关键词：吸波性能泊松比蜂窝构型优化设计蜂窝夹芯结构复合材料

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Aerodynamic interaction between forewing and hindwing of a hovering dragonfly

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Acta Mechanica Sinica 2014年第6期30卷 787-799页

作者： zheng hu Xin-Yan Deng School of Mechanical Engineering Purdue University 585 Purdue MallWest Lafayette IN 47906 USA

The phase change between the forewing and hindwing is a distinct feature that sets dragonfly apart from other *** this paper,we investigated the aerodynamic effects of varying forewing-hindwing phase di ff erence with a60 inclined stroke plane during hovering *** measurements on a pair of mechanical wing models showed that in-phase flight enhanced the forewing lift by 17%and the hindwing lift was reduced at most phase *** total lift of both wings was also reduced at most phase di ff erences and only increased at a phase range around *** results may explain the commonly observed behavior of the dragonfly where 0 is employed in *** further investigated the wing-wing interaction mechanism using the digital particle image velocimetry（PIV）system,and found that the forewing generated a downwash flow which is responsible for the lift reduction on the *** the other hand,an upwash flow resulted from the leading edge vortex of the hindwing helps to enhance lift on the *** results suggest that the dragonflies alter the phase di ff erences to control timing of the occurrence of flow interactions to achieve certain aerodynamic effects.

关键词： Dragonfly aerodynamics Forewing-hindwing interaction Phase di ff erence Interactive flow

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Test sequencing problem arising at the design stage for reducing life cycle cost

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Chinese Journal of Aeronautics 2013年第4期26卷 1000-1007页

作者： Zhang Shigang hu zheng Wen Xisen Laboratory of Science and Technology on Integrated Logistics Support College of Mechatronics and Automation National University of Defense Technology

Previous test sequencing algorithms only consider the execution cost of a test at the application stage. Due to the fact that the placement cost of some tests at the design stage is considerably high compared with the execution cost, the sequential diagnosis strategy obtained by previous methods is actually not optimal from the view of life cycle. In this paper, the test sequencing problem based on life cycle cost is presented. It is formulated as an optimization problem, which is non-deterministic polynomial-time hard （NP-hard）. An algorithm and a strategy to improve its computational efficiency are proposed. The formulation and algorithms are tested on various simulated systems and comparisons are made with the extant test sequencing methods. Application on a pump rotational speed control （PRSC） system of a spacecraft is studied in detail. Both the simulation results and the real-world case application results suggest that the solution proposed in this paper can significantly reduce the life cycle cost of a sequential fault diagnosis strategy.

关键词： AND/OR graph Heuristic search Life cycle cost Sequential fault diagnosis Test sequencing problem

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EBSD parameter assessment and constitutive models of crept HR3C austenitic steel

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Journal of Iron and Steel Research International 2023年第4期30卷 772-781页

作者： Jie Zhang zheng-fei hu Zhen Zhang Shanghai Key Laboratory for R&D and Application of Metallic Functional Materials School of Materials Science and EngineeringTongji UniversityShanghai 201804China School of Materials Science and Engineering Nanjing Institute of TechnologyNanjing 211167JiangsuChina

Creep damage and evolution of HR3C steel at 650℃ were investigated using electron backscatter diffraction(EBSD),and EBSD-based parameter assessments were *** analyses show that the grain size is almost unchanged and no obvious texture formed after creep at different creep *** lowest proportion of low Σ coincidence site lattice grain boundaries under 150 MPa implies that the primary twin structures are preserved under the low stress level,while some twin structures evolved into general grain boundaries at the high creep *** main damage features of microcracks and cavities can be seen along the grain boundaries:the former emerged at higher stress levels,while the latter appeared at the lower stress level,and both were shown under medium *** contrast shows that the most severe creep damage is present at 170 *** implies that the creep mechanism differs distinctly under different stress levels,and the transition point is around 170 *** average misorientation is better to describe the local plastic deformation related to the strain distribution while grain reference orientation deviation describes the inhomogeneous strain *** lifetime prediction models including the isothermal method,Larson-Miller parameter method and Monkman–Grant relation were evaluated by the experimental data and literature data,and they are valid for predicting creep behavior.

关键词： Austenitic heat-resistant steel Creep damage Electron backscatter diffraction Creep constitutive model Creep lifetime prediction

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Mesostructured carbon-based nanocages: an advanced platform for energy chemistry

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Science China Chemistry 2020年第5期63卷 665-681页

作者： Qiang Wu Lijun Yang Xizhang Wang zheng hu Key Laboratory of Mesoscopic Chemistry of Ministry of Education School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

The electrochemistry in energy conversion and storage(ECS) not only relies on the active species in catalysts or energy-storage materials, but also involves mass/ion transport around the active species and electron transfer to the external circuit. To realize high-rate ECS process, new architectures for catalysts or energy-storage electrodes are required to ensure more efficient mass/charge transport. 3 D porous mesostructured materials constructed by nanoscale functional units can form a continuous conductive network for electron transfer and an interconnected multiscale pores for mass/ion transport while maintaining the high surface area, showing great promise in boosting the ECS process. In this review, we summarize the recent progress on the design,construction and applications of 3 D mesostructured carbon-based nanocages for ECS. The role of the hierarchical architectures to the high rate performance is discussed to highlight the merits of the mesostructured materials. The perspective on future opportunities and challenges is also outlined for deepening and extending the related studies and applications.

关键词： carbon-based nanocages mesostructures energy conversion and storage enhanced mass/charge transport multifunction platform

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