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Direct AFM measurements of morphology and interaction force at solid-liquid interfaces between DTAC/CTAC and mica

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Journal of Central South University 2016年第9期23卷 2182-2190页

作者： XIE zhen JIANG Hao SUN Zhong-cheng YANG qin-hong School of Minerals Processing and Bioengineering Central South University Changsha 410083 China

The adsorption of dedecyltrimethylammoium chloride(DTAC) and hexadecyltrimethylammoium chloride(CTAC) on muscovite mica substrates was examined using atomic force microscopy(AFM). Adsorption morphology images and interaction forces of cationic surfactants at solid-solution interfaces were measured in tapping mode and Pico Force mode, respectively. The images demonstrated that the adsorbed structure was varied by a variety of surfactant concentrations. The adsorbed layer on mica was monolayer at first, and then became bilayer. A striped adsorbed structure was observed in a higher concentration of CTAC,which could not be found in any other concentrations of DTAC. For force measurements, the repulsive force was exponentially decreasing with the concentration increasing till a net attractive force appeared. A largest attractive force could be observed at a certain concentration, which was close to the point of charge neutralization. The results also showed a significant impact of hydrocarbon chain length on adsorption. An adsorption simulation was established to give a clear understanding of the interaction between cationic surfactants and mica.

关键词： adsorption cationic surfactants interaction force atomic force microscopy

分离富集磷酸化/糖基化蛋白质/肽的磁固相萃取新材料

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分析测试学报 2025年第1期44卷 1-11页

作者：鲁艳张森张锋葛鸿延庄琬月乔俊琴练鸿振生命分析化学国家重点实验室南京大学化学化工学院南京大学现代分析中心江苏南京210023

蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其... 详细信息

蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其进行分离富集成为蛋白质组学研究中决定性的步骤之一。磁固相萃取(MSPE)是以磁性纳米颗粒为吸附剂的固相萃取技术(SPE),较之常规SPE具有操作简单、环境友好、材料易于回收利用等优势,因而被广泛应用于磷酸化和糖基化蛋白/肽段等分析前的分离富集。该文对近5年文献中用于磷酸化和糖基化蛋白质分离富集的MSPE材料进行系统的归纳和评述,并对此类分离富集材料的未来发展方向进行了展望和探讨。

关键词：磷酸化蛋白质糖基化蛋白质磁固相萃取磁性纳米颗粒

Upreguiation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells

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World Journal of Gastroenterology 2005年第36期11卷 5627-5632页

作者： zhen-Liang Qu Sheng-Quan Zou Nai-Qiang Cui Xian-Zhong Wu Ming-Fang qin Di Kong zhen-Li Zhou Department of surgery Tianjin Nankai Hospital Tianjin 300100 China Department of Surgery Tongji Hospital Wuhan430030 Hubei Province China

AIM： To study the changes of human telomerase reverse transcriptase （hTERT） mRNA expression in human hepatocarcinoma cell lines （HepG2） and cholangiocarcinoma cell lines （QBC939） after HBx gene transfection and to illustrate the significance of transcriptional regulation of hTERT gene by HBx gene in the carcinogenesis. METHODS： HepG2 and QBC939 cell lines were cultured and co-transfected with eukaryotic expression vector containing the HBx coding region and cloning vector containing enhanced green fluorescent protein （EGFP） coding sequence using lipid-mediated gene transduction technique. Thirty-six hours after transfection, EGFP expression in cells was used as the indicator of successful transfection. Flow cytometry was performed to determine the transfection efficiency. Cells were harvested and total RNA was extracted using TRIzol reagent. The expression of hTERT mRNA in HepG2 and QBC939 cell lines was assayed by reverse transcriptionpolymerase chain reaction. The expression of HBx protein in both cell lines was detected by immunocytochemical staining and Western blotting. RESULTS： Flow cytometry showed that the transfection efficiency was 46.4% in HepG2 cells and 29.6% in QBC939 cells for both HBx gene expression vector and blank vector. The expression of hTERT mRNA was meaningfully increased in HepG2 and QBC939 cell lines when transfected with HBx gene expression vector compared to those transfected with OPTI-MEM medium and blank vector. Immunocytochemical staining and Western blotting revealed HBx protein expression in HepG2 and QBC939 cells only when transfected with HBx gene. CONCLUSION： HBx gene transfection can upregulate the transcriptional expression of hTERT mRNA. The transactivation of hTERT gene by HBx gene is a newfound mechanism for pathogenesis of hepatocarcinomas and cholangiocarcinomas after HBV infection. 2005 The WJG Press and Elsevier Inc. All rights reserved

关键词： Hepatocholangiocarcinoma Human telomerasereverse transcriptase Gene expression Hepatitis B virus X protein

Regulation of solvation structure and the cooperation environment of potassium bonds for wider-temperature adaptive potassium storage

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Rare Metals 2024年第4期43卷 1610-1621页

作者： Bing-Bing Liu Yi-Hui Liu You-Bin Zhang zhen-Guo Qi Guo-Hui qin State Key Laboratory Base for Eco-Chemical Engineering College of Chemical EngineeringQingdao University of Science and TechnologyQingdao266042China Shandong Synergetic Innovation Center of Ecological and Chemical Engineering Qingdao266042China

Antimony selenide(Sb_(2)Se_(3))is one of the perspective candidates for potassium-ion batteries due to its advanced virtues stem including featured high capacity,fertile reserves and the relative narrow band *** the unique advantages,it is still plagued by the unstable interface compatibility and poor wider-temperature *** optimization of microstructure and the construction of inorganic-organic hybrids with a low desolvation barrier and rapid kinetics behaviors are efficient to address these *** Sb_(2)Se_(3)nanorods enclosed by the S-doped carbon layer(SC),further crosslinked by the poly(N-isopropylacrylamide)(PM)film(PM@Sb_(2)Se_(3)@SC),were artificially fabricated,and it displays the enrichment ion aggregated model as well as contacted ion pair state,the well-tailored cooperation environment of potassium bonds,assuring a homogeneous potassium deposition and an excellent widertemperature *** complicated experimental studies and theoretical calculations authenticate the synergistic effects of geometric conformation and compositional design for the tremendously enhanced potassium ***,the full device over PM@Sb_(2)Se_(3)@SC anode and the potassium Prussian blue cathode manifests impressively durable cycling life and wider-temperature adaptability,verifying the glorious contribution from the finely manipulation in solvation structure and potassium bonds to enhancing the potassium storage behaviors.

关键词： Anode Sb_(2)Se_(3) rods Interface compatibility Inorganic-organic electrode Potassium ion battery

Effect of SiO_2 addition on the microstructure and electrical properties of ZnO-based varistors

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International Journal of Minerals,Metallurgy and Materials 2010年第1期17卷 86-91页

作者： zhen-hong Wu Jian-hui Fang Dong Xu qin-dong Zhong Li-yi Shi Research Center of Nano Science and Technology Shanghai University Shanghai 200444 China School of Science Shanghai University Shanghai 200444 China School of Material Science and Engineering Shanghai University Shanghai 200072 China

The microstructure and electrical properties of ZnO-based varistors with the SiO2 content in the range of 0-1.00mol% were prepared by a solid reaction route. The varistors were characterized by scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectrometry, inductively coupled plasma-atomic emission spectrometry, and X-ray photoelectron spectroscopy. The results indicate that the average grain size of ZnO decreases with the SiO2 content increasing. A new second phase （Zn2SiO4） and a glass phase （Bi2SiO5） are found. Element Si mainly exists in the grain boundary and plays an important role in controlling the Bi2O3 vaporization. The electric measurement shows that the incorporation of SiO2 can significantly improve the nonlinear properties of ZnO-based varistors, and the nonlinear coefficients of the varistors with SiO2 are in the range of 36.8-69.5. The varistor voltage reaches the maximum value of 463 V/mm and the leakage current reaches the minimum value of 0.11 μA at the SiO2 content of 0.75mol%.

关键词： inorganic materials varistor silicon dioxide electrical properties

Effect of Cesium Modification on CuO/CeO2 Catalysts for the Catalytic Decomposition of N2O

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Chemical Research in Chinese Universities 2019年第1期35卷 47-52页

作者： GUO Peiwen qin Feng SUN Chao HUANG zhen SHEN Wei XU Hualong Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Laboratory of Advanced Materials Collaborative Innovation Center of Chemistry for Energy MaterialsDepartment of Chemistry Fudan UniversityShanghai 200433P.R.China

A series of Cs-modified CuO/CeO2 mixed oxide catalysts was prepared for enhancing the stable activity of N2O *** was found that Cs modification promoted the catalytic performance of CuO/CeO2 catalysts significantly,The l%Cs-CuO/CeO2 catalyst exhibited the best activity,and the conversion of N2O reached 100%at 380℃ in the presence of 2% *** catalytic behaviors were investigated by means of XRD,N2 adsorption isotherms,XPS,H2-TPR(TPR:temperature-prograrmmed reduction),CO-IR,O2-TPD(TPD:temperature-programmed desorption)and diffused reflectance infrared Fourier transform spectorscopy(DRiFTs).The results revealed that Cs modification promoted the activity and the oxygen resistance by enhancing the desorption of surface oxygen species and increasing the content of Ce^3+.CO-DRIFTs revealed that Ce^3+could efficiently facilitate the regeneration of active Cu^+sites by an oxygen migration *** possible reaction mechanism was also discussed.

关键词： Cs-Modified CuO/CeO2 N2O decomposition Reaction mechanism

A Service Relevance Based Matching Algorithm on Grid Environment

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Wuhan University Journal of Natural Sciences 2006年第1期11卷 269-272页

作者： qin Wen-yan XU Li-zhen HE Jie-yue Department of Computer Science and Engineering Southeast University Nanjing 210096 Jiangsu China

This paper presents a semantic metadata description model for grid services, and furthermore, a service relevance based matching algorithm is proposed. Some rea sonable parameters interpreting services characterizations are considered to advise matching process, whose weightiness and service relevance dramatically contribute to the decision making of service matching degree, And what＇s more, matching is secondly executed on the bases of experience adaptation of matching factors, which improves matching degree in gird service retrieve greatly.

关键词： grid ontology semantic metadata service relevance match

Anti-oxidant effect of picroside II in a rat model of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2011年第15期6卷 1141-1146页

作者： Li Sun Xiaodan Li Ling Wang Lihua qin Yunliang Guo zhen Zhou Department of Neurology Second Affiliated Hospital of Qingdao University Medical College Qingdao 266043 Shandong Province China Institute of Cerebrovascular Diseases Qingdao University Medical College Qingdao 266021 Shandong Province China Department of Anatomy and Histoembryology Peking University Health Science Center Beijing 100191 China

Picroside II,the major active component of picroside,has been shown to induce PC12 cell axonal growth and relieve free radical *** vivo experiments have demonstrated that picroside II can improve neurological function in rats with cerebral ischemia/reperfusion *** the present in vivo study,enzyme-linked immunosorbent assay and immunohistochemistry revealed that picroside II increased superoxide dismutase content and reduced inducible nitric oxide synthase content in the ischemic *** effects of picroside II were similar to those of salvianic acid A sodium,an active control *** results indicate that picroside II exerts a neuroprotective effect,possibly by downregulating inducible nitric oxide synthase expression,increasing superoxide dismutase activity,and inhibiting neuronal apoptosis.

关键词： picroside II salvianic acid A sodium brain ischemia reperfusion injury apoptosis inducible nitric oxide synthase superoxide dismutase neural regeneration

Lipids, lipid metabolism and Kaposi's sarcoma-associated herpesvirus pathogenesis

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Virologica Sinica 2017年第5期32卷 369-375页

作者： Lu Dai zhen Lin Wei Jiang Erik K.Flemington Zhiqiang qin Department of Genetics Louisiana State University Health Sciences Center Louisiana Cancer Research Center New Orleans 70112 USA Department of Pediatrics East Hospital Tongji University School of Medicine Shanghai 200120 China Research Center for Translational Medicine and Key Laboratory of Arrhythmias East Hospital TongjiUniversity School of Medicine Shanghai 200120 China Department of Pathology Tulane University Health Sciences Center Tulane Cancer CenterNew Orleans 70112 USA Department of Microbiology and Immunology Division of Infectious Diseases Department of MedicineMedical University of South Carolina Charleston 29425 USA

Lipids are essential for mammalian cells to maintain many physiological functions. Emerging evidence has shown that cancer cells can develop specific alterations in lipid biosynthesis and metabolism to facilitate their survival and various malignant behaviors. To date, the precise role of cellular lipids and lipid metabolism in viral oncogenesis is still largely unclear with only a handful of literature covering this topic to implicate lipid metabolism in oncogenic virus associated pathogenesis. In this review, we focus on the role of lipid biosynthesis and metabolism in the pathogenesis of the Kaposi's sarcoma-associated herpesvirus, a common causative factor for cancers arising in the immunocompromised settings.

关键词： Kaposi’s sarcoma-associated herpesvirus(KSHV) herpesvirus lipid metabolism