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Extraordinary hot extreme in summer 2022 over the Yangtze River basin modulated by the La Nina condition under global warming

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Advances in Climate Change Research 2024年第1期15卷 21-30页

作者： zhen LIAO Yu-Feng YUAN Yang CHEN Pan-mao ZHAI State Key Laboratory of Severe Weather Chinese Academy of Meteorological SciencesBeijing 100081China

A widespread and prolonged hot extreme hit the Yangtze River basin in summer 2022,with 300 sites established new temperature records and nearly 96%stations endured more than 40 hot *** the perspective of the combination effect of the global warming and La Nina condition,potential mechanisms of the hot extreme were *** a record-breaking hot extreme was caused by an extremely strong and westward-shifted western Pacific subtropical high(WPSH).The global warming effect contributed primarily to the abnormal hot days in the Yangtze River basin,coupled with the modulation of the La Nina *** sea surface temperature anomaly pattern under La Nina condition favored more convection activities over the western Pacific,encouraging an enhanced and westward-extended *** addition,an observation-based attribution analysis indicates that anthropogenic warming may increase the probability of such extensively persistent hot extremeby1.8 times.

关键词： Hot extreme The Yangtze River basin Global warming Tropical Pacific forcing

肿瘤科护士道德勇气的潜在类别及影响因素分析

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护理学报 2024年第13期31卷 7-11页

作者：张一帆徐晓霞王子佼刘腊梅李珍英毛真雪郑州大学附属肿瘤医院河南省肿瘤医院放疗科河南郑州450008 郑州大学附属肿瘤医院河南省肿瘤医院护理部河南郑州450008 郑州大学护理与健康学院河南郑州450001

目的探讨肿瘤科护士道德勇气的潜在类别并分析其影响因素。方法2022年8—9月,采用便利抽样法选取郑州市某三级甲等医院502名肿瘤科护士为研究对象,采用一般资料调查表、护士道德勇气量表、心理韧性量表对其进行现况调查。采用Mplus 8.0... 详细信息

目的探讨肿瘤科护士道德勇气的潜在类别并分析其影响因素。方法2022年8—9月,采用便利抽样法选取郑州市某三级甲等医院502名肿瘤科护士为研究对象,采用一般资料调查表、护士道德勇气量表、心理韧性量表对其进行现况调查。采用Mplus 8.0进行潜在剖面分析,采用有序多分类Logistic回归分析评估各因素对护士道德勇气的影响。结果肿瘤科护士道德勇气可分为3个潜在类别,分别为:低水平道德勇气组(42.8%)、中等水平道德勇气组(39.9%)和高水平道德勇气组(17.3%),其影响因素包括年龄和心理韧性得分(均P<0.05)。结论肿瘤科护士道德勇气分为3个潜在类别,年龄、心理韧性是影响肿瘤科护士道德勇气的影响因素,为肿瘤科护士制定针对性的干预方案提供依据。

关键词：潜在剖面分析肿瘤科护士道德勇气影响因素

气相色谱串联质谱法测定茶叶中的5种农药残留

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现代食品 2024年第7期30卷 202-205页

作者：毛震刘璐贾松涛李梦雨赵林萍河南中标检测服务有限公司河南郑州450001

目的:建立一种气相色谱串联质谱法测定茶叶中水胺硫磷、联苯菊酯、毒死蜱、氯氰菊酯和高效氯氰菊酯、氰戊菊酯和S-氰戊菊酯5种农药残留量的分析方法。方法:样品经过Qu ECh ERS法前处理,通过气相色谱分离,多反应监测模式检测,内标法定量... 详细信息

目的:建立一种气相色谱串联质谱法测定茶叶中水胺硫磷、联苯菊酯、毒死蜱、氯氰菊酯和高效氯氰菊酯、氰戊菊酯和S-氰戊菊酯5种农药残留量的分析方法。方法:样品经过Qu ECh ERS法前处理,通过气相色谱分离,多反应监测模式检测,内标法定量。结果:5种农药在10~1000 ng·m L^(-1)线性关系良好,相关系数R^(2)均大于0.999。在0.05 mg·kg^(-1)、0.5 mg·kg^(-1)、1.0 mg·kg^(-1)3个加标水平下,回收率为80.2%~109.3%,RSD为1.2%~8.9%。结论:此方法具有操作简单、快速、灵敏度高、准确度高等优点,适用于茶叶中5种农药残留量的分析检测。

关键词：气相色谱串联质谱法茶叶农药残留

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Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment

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Nano Research 2024年第5期17卷 4270-4278页

作者： Xue mao zhen-zhen Li Dong-Lian Hao Wei-Dong Han Gao-Peng Li Yao-Yu Wang Kun Zhang School of Textile Science and Engineering Key Laboratory of Functional Textile Material and Product(Ministry of Education)Xi’an Polytechnic UniversityXi’an 710048China Key Laboratory of Magnetic Molecules&Magnetic Information Materials of the Ministry of Education School of Chemistry&Material ScienceShanxi Normal UniversityTaiyuan 030031China Department of Clinical Laboratory The Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710004China Shanghai iProteome Biotechnology Co. LtdShanghai 201210China College of Chemistry&Materials Science Northwest UniversityXi'an 710127China

Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches,but still a significant challenge in materials *** this end,we firstly constructed highly flexible ZrTiO_(4) nanofibrous membranes(NFMs)with excellent mechanical stability through a cost-effective and scalable electrospinning and subsequent calcination ***,to further increase the enrichment capacity of the phosphopeptide,the biomimetic TiO_(2)@ZrTiO_(4) NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO_(4) NFMs through growing unfurling TiO_(2) nanosheets onto the ZrTiO_(4) ***,remarkable flexibility and mechanical stability enable the resulting TiO_(2)@ZrTiO_(4) NFMs excellent practicability,while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer ***,6770 phosphopeptides can be enriched by TiO_(2)@ZrTiO_(4) NFMs(2205 corresponding phosphoproteins can be identified),and the value is much higher than that of ZrTiO_(4) NFMs(6399 phosphopeptides and 2132 identified phosphoproteins)and commercial high-performance TiO_(2) particles(4525 phosphopeptides and 1811 identified phosphoproteins).These results demonstrate the super ability of TiO_(2)@ZrTiO_(4) NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.

关键词： inorganic nanofibers flexible ceramic nanofibrous membranes biomimetic materials exceptional mechanical performance flexibility

Expression of mitogen-activated protein kinase double-stranded RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea

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Journal of Integrative Agriculture 2017年第10期16卷 2239-2245页

作者： CHEN Guo-hua TIAN Xue-liang WANG Dian-dong LING Jian mao zhen-chuan YANG Yu-hong XIE Bing-yan Institute of Vegetable and Flowers Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Genetic ImprovementMinistry of Agriculture College of Resources and Environment Henan Institute of Science and Technology Life Science and Technology Institute Yangtze Normal University

The expression of mitogen-activated protein kinase （mapk） double-stranded RNA in cucumber is effective in controlling infestations of the root-knot nematode Meloidogyne incognita. However, little is known about the ecological effects of transgenic plants. Here, we analyzed the diversity of 16S rDNA genes derived from the rhizosphere archaea of transgenic cucumber plants as an indicator of ecological change. A total of 17 and 18 operational taxonomic units were detected in the rhizospheres of non-transgenic cucumber and mapk dsRNA-expressing plants, respectively. No significant difference was observed between the two groups according to Shannon and Simpson indices. In soil samples of the two rhizospheres, the dominant group was Crenarchaeota at the phylum level, with Staphylothermus, Methanococcus, Pyrodictium and Sulfolobus the abundant taxa at the genus level. These results suggest that expressing mapk double-stranded （ds） RNA in cucumber has no apparent effect on the diversity of rhizosphere archaea, and provide powerful evidence for the ecological safety of transgenic cucumber expressing mapk dsRNA.

关键词： cucumber 16S rDNA diversity rhizosphere archaea mapk dsRNA

Bacterial artificial chromosome library construction of root-knot nematode resistant pepper genotype HDA149 and identification of clones linked to Me3 resistant locus

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Journal of Integrative Agriculture 2017年第1期16卷 57-64页

作者： GUO Xiao YANG Xiao-hui YANG Yu mao zhen-chuan LIU Feng MA Wei-qing XIE Bing-yan LI Guang-cun Institute of Vegetables and Flowers Shandong Academy of Agricultural SciencesMolecular Biology Key Laboratory of Shandong Facility Vegetable Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences

Pepper （Capsicum annuum. L.） is a widely cultivated vegetable crop worldwide and has the second largest planting area and the first largest vegetable output and value in China. Pepper root-knot nematode （Meloidogyne spp.） is one of the most serious pests of pepper, which caused huge losses every year. Previous studies showed that the Me3 gene is resistant to a wide range of Meloidogyne species, including M. arenaria, M. javanica, and M. incognita. HDA149, a double haploid pepper genotype, harboring the root-knot nematode resistance gene Me3, was used to construct bacterial artificial chro- mosome library （BAC） via the vector of CopyControFM pCC1 in this study. The library consists of 210 200 BAC clones and is equivalent to 5.3 pepper genomes. The average insert size is 95 kb, and most of them are 90-120 kb; but the empty clones are less than 3%. In order to screen the BAC library easily, 550 super pools with 384 BAC clones of each pool were further developed in this study. Specific primers from Me3 gene locus were used for BAC library screening, and more than 20 positive BAC clones were obtained. Then the selected positive BAC clones were analyzed by restriction enzyme digestion, BAC-end sequencing, marker development, and new positive BAC clones exploration, respectively. Finally, the contig with total length of about 300 kb linked to the Me3 locus was constructed based on chromosome walking strategy, which made a solid foundation for the cloning of the important root-knot nematode resistance gene Me3.

关键词： pepper bacterial artificial chromosome library.(BAC) root-knot nematode Me3 gene contig

Puerarin reduces increased c-fos, c-jun, and type Ⅳ collagen expression caused by high glucose in glomerular mesangial cells

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Acta Pharmacologica Sinica 2005年第8期26卷 982-986页

作者： Cai-ping mao, zhen-lun GU~1 Department of Pharmacology, Suzhou Institute of Chinese Materia Medica, Medical College of Soochow University Suzhou 215007, China Department of Pharmacology Suzhou Institute of Chinese Materia Medica Medical College of Soochow University Suzhou China

Aim: Increased expression of c-fos, c-jun and type Ⅳ collagen (CoⅣ) in glomerular mesangial cells (GMC) are important characteristics of diabetic nephropathy. Both c-los and c-jun regulate the gene expression of extracellular matrix components, and CoⅣ is the main component of the extracellular matrix. It has been reported that puerarin inhibits aggregation of the extracellular matrix in diabetic rats by an as yet unknown mechanism. The aim of this study is to investigate the effect of puerarin on c-fos, c-jun and CoⅣ expression in GMC cultured in medium containing 5.6 or 27.8 mmol/L glucose. Methods: The expressions of c-fos and c-jun were measured at the protein level using flow cytometry. CoⅣ content was detected using radioimmunoassay. Protein kinase C (PKC) activity was measured using liquid scintillation counting. Results: Puerarin (10-5mmol/L) significantly ameliorated the high-glucose effect on c-fos, c-jun and CoⅣ expression. This effect is accompanied by a reduced PKC activity in these cells. Coclusion: Our results suggest that reduced PKC activity and expression of c-fos and c-jun in GMC might participate in the mechanisms underlying the therapeutic effect of puerarin on diabetic nephropathy.

关键词： puerarin glomerular mesangial cells extracellular matrix diabetic nephropathy

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博兴洼陷沙四段沉积物源特征分析

矿物岩石 2011年第4期31卷 94-99页

作者：毛振强成都理工大学能源学院四川成都610059 中国石油化工胜利油田分公司纯梁采油厂山东博兴256504

论文依据博兴洼陷沙四段砂岩碎屑颗粒组分、泥质岩微量元素、重砂以及碎屑锆石同位素年代学分析资料,利用砂岩碎屑成分、重矿物分析、放射性元素等三种技术方法,建立了洼陷沙四段沉积复合体的高精度区域物源分析体系,论文将物源分布与... 详细信息

论文依据博兴洼陷沙四段砂岩碎屑颗粒组分、泥质岩微量元素、重砂以及碎屑锆石同位素年代学分析资料,利用砂岩碎屑成分、重矿物分析、放射性元素等三种技术方法,建立了洼陷沙四段沉积复合体的高精度区域物源分析体系,论文将物源分布与古地理特征、碎屑组分分布以及微量元素结合起来研究,探讨物源分配体系与储集层分布的关系,为非构造油气藏预测和勘探提供了重要参考价值,对类似沉积洼陷的物源体系研究具有借鉴意义。

关键词：物源砂岩组分判别重矿物锆石测年物源方向沙四段

A Meloidogyne incognita effector Minc03329 suppresses plant immunity and promotes parasitism

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Journal of Integrative Agriculture 2023年第3期22卷 799-811页

作者： ZHOU Jing-jing ZHANG Xiao-ping LIU Rui LING Jian LI Yan YANG Yu-hong XIE Bing-yan ZHAO Jian-long mao zhen-chuan State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Meloidogyne incognita is a devastating plant-parasitic *** play important roles during the stages of nematodes infection and parasitism,but their molecular functions remain largely *** this study,we characterized a new effector,Minc03329,which contains signal peptide for secretion and a C-type lectin *** yeast signal sequence trap experiments indicated that the signal peptide of Minc03329 is *** situ hybridization showed that Minc03329 was specifically expressed in the subventral esophageal ***-time qPCR confirmed that the expression level of Minc03329 transcript was significantly increased in pre-parasitic and parasitic second-stage juveniles(pre-J2s and par-J2s).Tobacco rattle virus(TRV)-mediated gene silencing of Minc03329 in host plants largely reduced the pathogenicity of *** the contrary,ectopic expression of Minc03329 in Arabidopsis thaliana significantly increased plant susceptibility to *** expression of Minc03329 in Nicotiana benthamiana leaves suppressed the programmed cell death triggered by the pro-apoptotic protein ***,the transcriptome analysis of Minc03329-transgenic Arabidopsis and wild type revealed that many defense-related genes were significantly ***,some different expressed genes were involved in the formation of nematode feeding *** results revealed that Minc03329 is an important effector for ***,suppressing host defense response and promoting pathogenicity.

关键词： Meloidogyne incognita effector C-type lectin pathogenicity plant immunity