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A review of research progress on continuous cropping obstacles

Frontiers of Agricultural Science and Engineering 2024年第2期11卷 253-270页

作者： Kunguang WANG Qiaofang LU zhechao dou Zhiguang CHI Dongming CUI Jing MA Guowei WANG Jialing KUANG Nanqi WANG Yuanmei ZUO College of Resources and Environmental Sciences State Key Laboratory of Nutrient Use and ManagementNational Academy of Agriculture Green DevelopmentChina Agricultural UniversityBeijing 100193China College of Resources and Environmental Sciences Southwest UniversityChongqing 400715China Yunnan ICL YTH Phosphate Research and Technology Co. Ltd.Kunming 650228China

Due to the increasing global population and limited land resources, continuous cropping has become common. However, after a few years of continuous cropping, obstacles often arise that cause soil degeneration, decreased crop yield and quality, and increased disease incidence, resulting in significant economic losses. It is essential to understand the causes and mitigation mechanisms of continuous cropping obstacles(CCOs) and then develop appropriate methods to overcome them. This review systematically summarizes the causes and mitigation measures of soil degradation in continuous cropping through a meta-analysis. It was concluded that not all continuous cropping systems are prone to CCOs. Therefore, it is necessary to grasp the principles governing the occurrence of diseases caused by soilborne pathogens in different cropping systems, consider plant–soil-organisms interactions as a system, scientifically regulate the physical and chemical properties of soils from a systems perspective, and then regulate the structure of microbial food webs in the soil to achieve a reduction in diseases caused by soilborne pathogens and increase crop yield ultimately. This review provides reference data and guidance for addressing this fundamental problem.

关键词： Continuous cropping obstacles rhizosphere regulation soil microecological environment

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A review of soil nematodes as biological indicators for the assessment of soil health

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Frontiers of Agricultural Science and Engineering 2020年第3期7卷 275-281页

作者： Qiaofang LU Tongtong LIU Nanqi WANG zhechao dou Kunguang WANG Yuanmei ZUO College of Resources and Environmental Sciences National Academy of Agriculture Green DevelopmentKey Laboratory of Plant-Soil Interactions(Ministry of Education)China Agricultural UniversityBeijing 100193China

Healthy soils are essential for sustainableagricultural development and soil health requires carefulassessment with increasing societal concern over envir-onmentally friendly agricultural development. Soil healthis the capacity of soil to function within ecologicalboundaries to sustain productivity, maintain environmentalquality, and promote plant and animal health. Physical,chemical and biological indicators are used to evaluate soilhealth;the biological indicators include microbes, proto-zoa and metazoa. Nematodes are the most abundantmetazoa and they vary in their sensitivity to pollutants andenvironmental disturbance. Soil nematode communitiesare useful biological indicators of soil health, withcommunity characteristics such as abundance, diversity,community structure and metabolic footprint all closelycorrelated with the soil environment. The community size,complexity and structure reflect the condition of the *** free-living and plant parasitic nematodes are effec-tive ecological indicators, contributing to nutrient cyclingand having important roles as primary, secondary andtertiary consumers in food webs. Tillage inversion,cropping patterns and nutrient management may havestrong effects on soil nematodes, with changes in soilnematode communities reflecting soil disturbance. Somefree-living nematodes serve as biological models to testsoil condition in the laboratory and because of theseadvantages soil nematodes are increasingly being used asbiological indicators of soil health.

关键词： biological indicators community characteristics soil health soil nematodes

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CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry

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Journal of Genetics and Genomics 2018年第12期45卷 685-687页

作者： Sinian Xing Meiru Jia Lingzhi Wei Wenwen Mao Usman Ali Abbasi Yaoyao Zhao Yating Chen Minglin Cao Kai Zhang Zhengrong Dai zhechao dou Wensuo Jia Bingbing Li College of Horticulture China Agricultural University

CRISPR/Cas9-mediated genome editing is a powerful tool for life science research. Recently, strawberry (Fragaria × ananassa), an important horticultural crop, has emerged as a model organism for investigating the regulatory mechanisms of fruit development and ripening (Shulaev et al., 2011; Jia et al., 2013, 2017; Kang et al., 2013; Han et al., 2015). While most cultivated strawberries

关键词： PDS CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry

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Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits

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Horticulture Research 2019年第1期6卷 1043-1055页

作者： Yaoyao Zhao Wenwen Mao Yating Chen WeiWang Zhengrong Dai zhechao dou Kai Zhang Lingzhi Wei Tianyu Li Baozhen Zeng Ting Liu Yijuan Fan Jiaqi Yan Bingbing Li Wensuo Jia College of Horticulture China Agriculture UniversityBeijingCN 100193P.R.China

Strawberry is increasingly used as a model plant for research on fruit growth and *** transient gene manipulation(TGM)technique is widely used to determine the function of plant genes,including those in strawberry ***,its reliable application for the precise identification of gene function has been difficult owing to the lack of conditional *** this study,we found that successful transient gene manipulation requires optimization,with the vector type,temperature,and fruit developmental stage being three major factors determining ***,we found that transient gene manipulation was feasible only from the large green fruit stage onwards,making it especially suitable for identifying genes involved in strawberry fruit ***,we established a method called percentage difference of phenotype(PDP),in which the functional effect of a gene could be precisely and efficiently identified in strawberry *** method can be used to estimate the functional effect of a gene as a value from 0 to 100%,such that different genes can be quantitatively compared for their relative abilities to regulate fruit *** study provides a useful tool for accelerating research on the molecular basis of strawberry fruit ripening.

关键词： straw transient functional

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生物源新型铁螯合剂研究进展及其应用

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华中农业大学学报 2023年第6期42卷 59-72页

作者：崔冬明单晨史利桦王坤光豆哲超迟志广王国炜匡家灵左元梅养分利用和管理国家重点实验室/中国农业大学资源环境学院/国家农业绿色发展研究院北京100193 西南大学资源环境学院重庆400715 云南云天化股份有限公司研发中心昆明650228

铁是所有生物正常发育所必需的微量元素之一,尤其是通过生物强化培育富含铁营养的农产品是解决人类铁营养“隐性饥饿”的重要技术途径,而螯合态铁肥由于见效快被广泛应用。因此,创制和研发新型微量元素螯合剂始终是国内外研究的竞争热... 详细信息

铁是所有生物正常发育所必需的微量元素之一,尤其是通过生物强化培育富含铁营养的农产品是解决人类铁营养“隐性饥饿”的重要技术途径,而螯合态铁肥由于见效快被广泛应用。因此,创制和研发新型微量元素螯合剂始终是国内外研究的竞争热点。麦根酸类植物铁载体和微生物铁载体等,能够高效螯合难溶铁并被植物高效吸收利用,是潜在的新型生物源螯合剂。该类新型铁肥在改善植物铁营养的同时不需要外源铁的投入,而是发挥菌株自身活性物质较强的螯合特性高效活化土壤中丰富的铁资源,为植物提供足够的生物有效铁。为进一步挖掘和研发新型生物源的绿色、高效且稳定的螯合剂,并为绿色智能肥料研发、实现绿色农业的可持续发展提供新途径和技术突破,本文基于近年来植物和微生物对铁营养吸收利用的分子生理机制的不断深入研究,从植物缺铁现状及诱因、改善铁营养的途径,到机理Ⅱ植物根系分泌物吸收利用铁的分子生态优势以及微生物铁载体改善植物铁营养的潜力,对生物源新型铁螯合剂研究进展及其应用进行了系统综述。期望通过进一步的研究和开发,能够更深入地了解这些新型生物源螯合剂的作用机制以提高植物的铁营养吸收效率,为实现绿色农业的可持续发展提供新的解决方案。

关键词：铁营养生物强化生物源螯合剂植物铁载体微生物铁载体 “隐性饥饿”

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