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Long non-coding RNA AFAP1-AS1 accelerates lung cancer cells migration and invasion by interacting with SNIP1 to upregulate c-Myc

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Fang Wei Qianjin Liao Wenling Zhang Wei Xiong zhaoyang zeng The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health Xiangya HospitalCentral South University The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education Cancer Research InstituteCentral South University Hunan Key Laboratory of Nonresolving Inflammation and Cancer Disease Genome Research Centerthe Third Xiangya HospitalCentral South University Hunan Key Laboratory of Translational Radiation Oncology Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of MedicineCentral South University

Objective: BPI fold containing family B member1(BPIFB1, previously termed LPLUNC1), a secreted protein highly expressed in nasopharynx, is significantly down-regulated in nasopharyngeal carcinoma(NPC). Thus, we investigated whether the downregulated BPIFB1 expression is associated with the prognosis of NPC. Considering that metastasis represents the primary cause of death in these patients, here we explored the role of BPIFB1 in NPC migration and invasion. Methods: The expression and clinical associations of BPIFB1 in NPC tissues were analyzed by immunohistochemistry. The effect of BPIFB1 on NPC metastasis was investigated in vitro and in vivo. A co-IP assay coupled with mass spectrometry approach was used to identified BPIFB1 binding proteins. To investigate whether BPIFB1 inhibit metastasis by interacting with vitronectin(VTN) and vimentin(VIM), we used western blot, immunofluorescence, and immunohistochemistry analysises to assess the underlying molecular mechanisms. Results: Little or no BPIFB1 expression was detected in NPC tissues, and the levels of BPIFB1 expression in the NPC were associated positively with the survival of NPC patients. In vitro and in vivo experiments showed that re-expression of BPIFB1 markedly inhibited the migratory, invasive and lung metastasis capability of NPC cells. We identified two BPIFB1-interacting proteins, the cell adhesion protein VTN and the intermediate filament protein VIM. BPIFB1 could reduce the expression of VTN and the formation of a VTN-ITGAV complex in NPC cells, leading to inhibition of the FAK-SRC-ERK signaling pathway. It could also attenuate the migration and invasion of NPC by inhibiting the VTN-or VIM-induced EMT process of NPC cells. Conclusion: BPIFB1 expression is negatively correlated with the clinical stages of NPC, suggesting that the reduction in BPIFB1 expression constitutes a novel adverse prognostic factor in NPC. And this research represents the first demonstration of BPIFB1 function in NPC migration,

关键词： nasopharyngeal carcinoma BPIFB1 LPLUNC1 VTN VIM metastasis

来源： cnki会议评论

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Signal Transduction and Targeted Therapy 2021年第7期6卷 2225-2237页

作者： Yu Zhong Liting Yang Fang Xiong Yi He Yanyan Tang Lei Shi Songqing Fan Zheng Li Shanshan Zhang Zhaojian Gong Can Guo Qianjin Liao Yujuan Zhou Ming Zhou Bo Xiang Xiaoling Li Yong Li zhaoyang zeng Guiyuan Li Wei Xiong NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism Hunan Cancer Hospital and the Affiliated Cancer HospitalXiangya School of MedicineCentral South UniversityChangshaHunanChina Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education Cancer Research Institute and School of Basic Medical ScienceCentral South UniversityChangshaHunanChina Department of Neurosurgery Xiangya HospitalCentral South UniversityChangshaHunanChina Department of Oral and Maxillofacial Surgery the Second Xiangya HospitalCentral South UniversityChangshaHunanChina Department of Medicine Dan L Duncan Comprehensive Cancer CenterBaylor College of MedicineHoustonTXUSA

Actin filament associated protein 1 antisense RNA 1(named AFAP1-AS1)is a long non-coding RNA and overexpressed in many *** study aimed to identify the role and mechanism of AFAP1-AS1 in lung *** AFAP1-AS1 expression was firstly assessed in 187 paraffin-embedded lung cancer and 36 normal lung epithelial tissues by in situ *** migration and invasion abilities of AFAP1-AS1 were investigated in lung cancer *** uncover the molecular mechanism about AFAP1-AS1 function in lung cancer,we screened proteins that interact with AFAP1-AS1 by RNA pull down and the mass spectrometry ***1-AS1 was highly expressed in lung cancer clinical tissues and its expression was positively correlated with lung cancer patients'poor *** vivo experiments confirmed that AFAP1-AS1 could promote lung cancer ***1-AS1 promoted lung cancer cells migration and invasion through interacting with Smad nuclear interacting protein 1(named SNIP1),which inhibited ubiquitination and degradation of c-Myc *** of c-Myc molecule in turn promoted the expression of ZEB1,ZEB2,and SNAIL gene,which ultimately enhanced epithelial to mesenchymal transition(EMT)and lung cancer *** the molecular mechanism by which AFAP1-AS1 promotes lung cancer's migration and invasion may provide novel therapeutic targets for lung cancer patients'early diagnosis and therapy.

关键词： invasion lung metastasis

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高碾压混凝土拱坝中的构造缝问题研究

水力发电 1998年第2期24卷 30-33页

作者：曾昭扬马黔清华大学

为了控制施工期温降引起的坝体裂缝，国内外已建成的几座碾压混凝土拱坝普遍设置了诱导缝。我国在国家“八五”科技攻关过程中深入研究了诱导缝的作用和合理布置问题，提出了诱导缝等效强度计算模型和断裂判别式以及有效作用范围估计方... 详细信息

为了控制施工期温降引起的坝体裂缝，国内外已建成的几座碾压混凝土拱坝普遍设置了诱导缝。我国在国家“八五”科技攻关过程中深入研究了诱导缝的作用和合理布置问题，提出了诱导缝等效强度计算模型和断裂判别式以及有效作用范围估计方法，为诱导缝设计提供了理论依据，并对高碾压混凝土拱坝设置少量横缝的作用和适用条件提出了依据。

关键词：构造缝诱导缝高碾压混凝土拱坝

基于R语言数据挖掘中药治疗糖尿病足溃疡的用药规律探析

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中国民族民间医药 2024年第2期33卷 112-118页

作者：周丁鹏何於成李开燕曾昭洋甘肃中医药大学甘肃兰州730000

目的:基于R语言数据挖掘平台对数据库中糖尿病足溃疡的方药信息进行数据挖掘,丰富中医药治疗糖尿病足溃疡的方法和思路。方法:计算机检索中国知网、中国生物医学、维普、万方、PubMed共5个数据库。筛选出治疗糖尿病足溃疡的相关文献。利... 详细信息

目的:基于R语言数据挖掘平台对数据库中糖尿病足溃疡的方药信息进行数据挖掘,丰富中医药治疗糖尿病足溃疡的方法和思路。方法:计算机检索中国知网、中国生物医学、维普、万方、PubMed共5个数据库。筛选出治疗糖尿病足溃疡的相关文献。利用Excel软件提取汇总所有的方药,采用R语言软件对中药使用频次、相关性、关联度和聚类等方面进行挖掘分析。对核心方剂进行网络药理学分析和分子对接技术进行验证。结果:筛选出治疗糖尿病足溃疡的323首经验方药,对筛选出的方药进行中药使用频次统计,得出其中使用频次>90次的药物依次为当归、黄芪、牛膝、丹参、赤芍、川芎、红花、玄参及甘草,相关系数较高的前20味药对组合多为补虚药,通过关联分析和聚类分析形成以补益气血药、清热解毒药及活血化瘀药为基础的核心药物组方。结论:治疗糖尿病足溃疡的中药组方多为自拟方,其治法以补益气血、清热解毒、活血化瘀为主。药物是以补虚类,清热解毒、凉血类及活血化瘀类为主。

关键词： R语言数据挖掘糖尿病足中药组方用药规律

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CAS-ESM2.0 Dataset for the Carbon Dioxide Removal Model Intercomparison Project(CDRMIP)

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Advances in Atmospheric Sciences 2024年第5期41卷 989-1000页

作者： Jiangbo JIN Duoying JI Xiao DONG Kece FEI Run GUO Juanxiong HE Yi YU zhaoyang CHAI He ZHANG Dongling ZHANG Kangjun CHEN Qingcun zeng International Center for Climate and Environment Sciences Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China State Key Laboratory of Earth Surface Processes and Resource Ecology College of Global Change and Earth System ScienceBeijing Normal UniversityBeijing 100875China State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China

Understanding the response of the Earth system to varying concentrations of carbon dioxide(CO_(2))is critical for projecting possible future climate change and for providing insight into mitigation and adaptation strategies in the near *** this study,we generate a dataset by conducting an experiment involving carbon dioxide removal(CDR)—a potential way to suppress global warming—using the Chinese Academy of Sciences Earth System Model version 2.0(CASESM2.0).A preliminary evaluation is *** model is integrated from 200–340 years as a 1%yr^(−1) CO_(2) concentration increase experiment,and then to~478 years as a carbon dioxide removal experiment until CO_(2) returns to its original ***,another 80 years is integrated in which CO_(2) is kept *** in the 2-m temperature,precipitation,sea surface temperature,ocean temperature,Atlantic meridional overturning circulation(AMOC),and sea surface height are all *** the ramp-up period,the global mean 2-m temperature and precipitation both increase while the AMOC *** of all the above variables change in the opposite direction in the ramp-down period,with a delayed peak relative to the CO_(2) *** CO_(2) returns to its original value,the global mean 2-m temperature is still~1 K higher than in the original state,and precipitation is~0.07 mm d^(–1) *** the end of the simulation,there is a~0.5°C increase in ocean temperature and a 1 Sv weakening of the *** model simulation produces similar results to those of comparable experiments previously reported in the literature.

关键词： CAS-ESM2.0 CDRMIP carbon dioxide removal AMOC temperature precipitation sea surface height

调频连续波激光雷达目标相对距离及径向速度信息提取方法

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航空学报 2014年第2期35卷 523-531页

作者：杜小平宋一铄曾朝阳装备学院航天指挥系北京101416 装备学院研究生院北京101416 装备学院光电装备系北京101416

利用线性调频连续波(LFM/CW)激光雷达进行测量时,相对运动引起的多普勒效应和信息输出延迟都会造成测量结果偏差。分析了多普勒效应和信息输出延迟对测量的影响,提出了基于偏差抵消原理的相对距离及径向速度信息提取方法,根据测量任务... 详细信息

利用线性调频连续波(LFM/CW)激光雷达进行测量时,相对运动引起的多普勒效应和信息输出延迟都会造成测量结果偏差。分析了多普勒效应和信息输出延迟对测量的影响,提出了基于偏差抵消原理的相对距离及径向速度信息提取方法,根据测量任务需求对该方法和基于二维傅里叶变换的信息提取方法进行了仿真比较。结果表明:基于偏差抵消的信息提取方法不仅可以在相对径向速度未知的情况下准确提取目标相对距离信息,更可在每个调频周期结束后给出测距结果从而提高测距实时性;相比基于二维傅里叶变换的信息提取方法,当相对径向速度小于750 m/s时,基于偏差抵消的信息提取方法可使得测速偏差减小一个数量级。

关键词：调频连续波激光雷达多普勒效应偏差抵消相对测量

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基于特征空间显著性的假目标光谱设计

光学学报 2017年第1期37卷 293-299页

作者：李姝颖杜山山曾朝阳解放军理工大学野战工程学院国防科技重点实验室江苏南京210007

为解决高光谱探测条件下的假目标光谱设计问题,从特征空间中目标显著性的角度分析了假目标的设计原则。区别于其他目标探测,高光谱目标探测是高维数据的处理过程,目标显著性反映在数据经降维处理后形成的特征子空间中。据此提出的假目... 详细信息

为解决高光谱探测条件下的假目标光谱设计问题,从特征空间中目标显著性的角度分析了假目标的设计原则。区别于其他目标探测,高光谱目标探测是高维数据的处理过程,目标显著性反映在数据经降维处理后形成的特征子空间中。据此提出的假目标设计原则如下。先在特征子空间内设计假目标,使其一方面凸显自身,另一方面则遮掩真目标。而后依据特征子空间与光谱通道的线性关系,通过子空间中假目标与其他目标的相对位置确定假目标的光谱特性。对相关图像的检测表明,这样设计的假目标能够有效降低真实目标的探测效果。相关结论对高光谱隐身技术有一定指导作用。

关键词：遥感高光谱目标探测特征空间显著性

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PHM技术在航空结构疲劳寿命预测中的应用

机械强度 2017年第2期39卷 435-440页

作者：宋博祝青钰曾照洋中国航空综合技术研究所北京100028

飞机机体结构疲劳失效几乎都发生在紧固件孔的孔边位置,结构疲劳断裂会导致飞机出现灾难性的事故,PHM技术可用于预报疲劳故障何时可能发生,从而实现自助式保障,降低使用和保障费用。基于失效物理模型,建立连接件疲劳寿命的预测方法,方... 详细信息

飞机机体结构疲劳失效几乎都发生在紧固件孔的孔边位置,结构疲劳断裂会导致飞机出现灾难性的事故,PHM技术可用于预报疲劳故障何时可能发生,从而实现自助式保障,降低使用和保障费用。基于失效物理模型,建立连接件疲劳寿命的预测方法,方法首先求解含孔构件的应力严重系数和疲劳缺口系数,然后对应变-寿命曲线的弹性段进行了修正使局部应力应变法同时适用于高、低周疲劳寿命的预测。结果表明,建立的疲劳寿命预测方法的计算结果与疲劳试验结果相比误差小于17%,说明了方法的有效性。

关键词： PHM 应力严重系数局部应力应变法疲劳寿命

Long non-coding RNA LOC284889 promotes the formation and progression of nasopharyngeal carcinoma

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Long non-coding RNA LOC284889 promotes the formation and pro...