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One-stitch anastomosis through the skin with bicanalicular intubation:a modified approach for repair of bicanalicular laceration

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International Journal of Ophthalmology(English edition) 2013年第5期6卷 656-658页

作者： Hai Tao Peng Wang Cui Han Jian Zhang Fang Bai zhao-yan he Lacrimal Center of Ophthalmology Armed Police General Hospital of China

AIM:To evaluate the efficacy and safety of one-stitch anastomosis through the skin with bicanalicular silicone tube intubation in repairing of bicanalicular ***:The clinical data of 15 consecutive patients with both superior and inferior canalicular laceration in one eye who underwent surgical repair using one-stitch anastomosis through the skin and bicanalicular stent were retrospective *** the operations were performed under surgical microscope,5-0 silk sutures were used and were with bicanalicular silicone tube(diameter was 8mm) intubation,for one lacerated canaliculi one-stitch anastomosis through the *** stents were left in place for 3 months postoperatively and then *** follow-up period was 3-36 months(average 14 months). RESULTS:In 15 patients,13 patients were cured entirely,1 patient was meliorated,1 patient with no *** patients had got good recovery of eyelid laceration with no traumatic deformity in eyelid and *** was seen in one case,for not followed the doctor’s guidance to come back to hospital to had the suture removed on the 7thday after operation,when he came at the 15thday,the inferior canalicular wall and eyelid skin were corroded by the suture caused 2mm wound,and the inside silicone tube was exposed,a promptly repair with 10-0 nylon suture was done,the wound healed in a *** were no early tube protrusions and punctal slits in the ***:One-stitch anastomosis through the skin with bicanalicular silicone tube intubation is a good method in repair of bicanalicular laceration in one eye,the cut ends can be anastomosed directly,and with excellent cosmetic results,it is acceptable for the *** there is no suture remained in the wound permanently,so there is no suture-related granuloma which may cause obstruction or stenosis of *** is simple,economical,effective and safe.

关键词： both superior and inferior canalicular laceration repair one-stitch anastomosis through the skin bicanalicular intubation

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Identification and Promoter Activity Analysis of Porcine miR-181 and miR-1

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Journal of Integrative Agriculture 2012年第6期11卷 986-992页

作者： ZHANG Hai-xin ZHANG Rui LIU Yi-nan WANG Dao-lin zhao yan-he REN Zhu-qing XIONGYuan-zhu Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture/Key Laboratory of Agriculture Animal Genetics Breeding and Reproduction Ministry of Education/College of Animal Science Huazhong Agricultural University Wuhan 430070 P.R. China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 P.R.China

Since its discovery a decade ago, microRNA has been identified as one of the major regulatory gene families in eukaryotic cells. Many functions of microRNAs have been revealed both in flora and fauna in recent years, but the transcriptional regulation of microRNA genes is not well-understood. In the present study, a series of primers were designed in the 2 000 nt upstream regions of porcine miR-181 and miR-1 and then the sequences were cloned into pGL3-basic vector to test their transcriptional activity. Dual-luciferase reporter assays showed that, the activity of 5＂-flanking sequence of miR-181 started on construct -51, decreasing with the length of the fragment up to -444. The upstream 590 bp confer maximal transcriptional activity and the basal promoter activity is located within the -82 to ＋16 bp region. For miR-1, the activity starts on construct -50, decreasing with the length of the fragment up to -1 254 in despite of a bit of fluctuation, and the basal promoter activity is located within the -50 to ＋47 bp region. Furthermore, some putative regulation elements of both miR-181 and miR-1 were located. In addition, tissue distribution revealed that miR-181 is expressed at a relatively low level.

关键词： miR-181 miR-1 pigs promoter transcriptional activity

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Multifunctional Nacre‑Like Nanocomposite Papers for Electromagnetic Interference Shielding via heterocyclic Aramid/MXene Template‑Assisted In‑Situ Polypyrrole Assembly

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Nano-Micro Letters 2025年第3期17卷 37-54页

作者： Jinhua Xiong Xu zhao Zonglin Liu he Chen Qian yan Huanxin Lian Yunxiang Chen Qingyu Peng Xiaodong he National Key Laboratory of Science and Technology On Advanced Composites in Special Environments Center for Composite Materials and StructuresHarbin Institute of TechnologyHarbin 150080People’s Republic of China

Robust, ultra-flexible, and multifunctional MXene-basedelectromagnetic interference (EMI) shielding nanocomposite filmsexhibit enormous potential for applications in artificial intelligence,wireless telecommunication, and portable/wearable electronic *** this work, a nacre-inspired multifunctional heterocyclic aramid(HA)/MXene@polypyrrole (PPy) (HMP) nanocomposite paper withlarge-scale, high strength, super toughness, and excellent tolerance tocomplex conditions is fabricated through the strategy of HA/MXenehydrogel template-assisted in-situ assembly of PPy. Benefiting from the"brick-and-mortar" layered structure and the strong hydrogen-bondinginteractions among MXene, HA, and PPy, the paper exhibits remarkable mechanical performances, including high tensile strength (309.7 MPa),outstanding toughness (57.6 MJ m−3), exceptional foldability, and structural stability against ultrasonication. By using the template effect ofHA/MXene to guide the assembly of conductive polymers, the synthesized paper obtains excellent electronic conductivity. More importantly,the highly continuous conductive path enables the nanocomposite paper to achieve a splendid EMI shielding effectiveness (EMI SE) of 54.1 dBat an ultra-thin thickness (25.4 μm) and a high specific EMI SE of 17,204.7 dB cm2g−1. In addition, the papers also have excellent applicationsin electromagnetic protection, electro-/photothermal de-icing, thermal therapy, and fire safety. These findings broaden the ideas for developinghigh-performance and multifunctional MXene-based films with enormous application potential in EMI shielding and thermal management.

关键词： MXene Remarkable mechanical properties heterocyclic aramid Electromagnetic interference shielding Polypyrrole Multifunctionality

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Identification of Residue Involved in Nucleotidyltransferase Activity of LinA from Staphylococci by Site-directed Mutagenesis

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Chinese Journal of Structural Chemistry 2019年第3期38卷 422-428页

作者： ZOU Xiao-Ming JIANG Mei-Qin zhao yan-he SUN Li-Fang yanG Wen-Di YE Xian-Ren WU Yun-Kun State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Department of Cell Biology and Biophysics University of Texas Southwestern Medical Center Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation College of Life Science Fujian Normal University Fujian Cancer Hospital & Fujian Medical University Cancer Hospital

The lincosamide class of antibacterials is widely used for the treatment of a broad spectrum of infections, and one prevalent route of resistance to lincosamides in pathogenic gram-positive cocco is antibiotic modification. Enzymes encoded by lin genes, belonging to nucleotidyltransferase superfamily, catalyze adenylylation to inactivate lincosamides. LinA can adenylylate lincosamides at either 3?-or 4?-OH of the methylthiolincosamide sugar. The crystal structure of LinA/lincomycin has confirmed its active site. However, the residue interacting with nucleotidyl donors remains elusive. here, we modeled the complex structure of LinA/lincomycin/Mg^(2+)/AMPCPP to reveal a putative pocket for nucleotidyl donors and suggested the residue R45 in this pocket involved in the recognition of donor substrates NTP and catalysis. ITC and enzyme activity assays show that the mutation of residue R45 impairs LinA nucleotidyltransferase activity in vitro. This work provides insights into the molecular mechanism of the nucleotide binding and transferring activity of antibiotic NTases.

关键词： LinA nucleotidyltransferase site-directed mutagenesis enzymatic assay ITC

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Gradient maturation experiment on hairs implies taphonomic changes in fossil hairs

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Science China Earth Sciences 2025年第1期68卷 62-73页

作者： Xin LI Yibo ZHOU Tao zhao Xingjian he yanhong PAN State Key Laboratory for Mineral Deposits Research Center for Critical Earth Material Cycling Centre for Research and Education on Biological Evolution and Environment and Frontiers Science School of Earth Sciences and Engineering Nanjing University Institute of Palaeontology Yunnan Key Laboratory of Earth System Science Yunnan Key Laboratory for PalaeobiologyYunnan University

Mammalian hair, as a key feature related to endothermy, physical protection, and camouflage, has a long geohistorical record that dates back even to the Mesozoic. However, the limited information on the taphonomy of hair hinders the understanding of its evolutionary significance in deep time. To address this, we conducted taphonomic experiments to simulate the transformation process of black hair. The changes in the morphological structure, microscopic characteristics, and chemical composition of hair during the simulated fossilization process were documented by scanning electron microscopy, Fourier transform infrared(FTIR) spectroscopy, and Raman spectroscopy. Our results revealed that damage to the morphology of hair began at a mild temperature(150–175°C), and hair transformed into a fluid-like mass at a relatively high temperature(200°C). In addition, the morphological structure of melanosomes had relatively greater preservation potential than keratins did, and the microscopic features of melanosomes were persistently stable. The Raman and FTIR spectra indicated that the characteristic melanin and keratin peaks changed as the temperature increased. Although the chemical signals were not lost completely, the variations in the characteristic amide bands in the FTIR spectra indicate that the keratins severely degraded at high temperatures(>200°C), whereas the characteristic melanin bands in the Raman spectra suggested that melanin signals were still recognizable, even at high temperatures(>200°C). These results were confirmed by principal component analysis(PCA) and principal component analysis linear discriminant analysis(PCA–LDA). In addition, the Raman signals of all the samples in the simulated experiments were distinguished from the signals of common geological carbonaceous materials(humic acid), which suggested that Raman spectroscopy can be used to detect melanin in fossils.

关键词： Mammalian hair Melanin Keratin Fossilization Experimental taphonomy

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备用信用证担保人民币贷款的性质、条件及风险控制

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税务与经济 2006年第1期 83-86页

作者：赵延河深圳发展银行北京分行国际部北京100031

备用信用证贷款是由开证行承担独立、第一性付款责任的外汇、信用保证贷款,其担保的有效性决定了融资业务的低风险特征。认识备用证及其担保作用,明确业务性质,是确立信贷条件和有效控制风险的必要前提,也是规范业务发展的基本要求。

关键词：备用信用证贷款风险控制担保

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IDSSCNN-XgBoost:Improved Dual-Stream Shallow Convolutional Neural Network Based on Extreme Gradient Boosting Algorithm for Micro Expression Recognition

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Computers, Materials & Continua 2025年第1期82卷 729-749页

作者： Adnan Ahmad zhao Li Irfan Tariq Zhengran he School of Information Science and Engineering Southeast UniversityNanjing210016China

Micro-expressions(ME)recognition is a complex task that requires advanced techniques to extract informative features fromfacial *** deep neural networks(DNNs)with convolutional structures have been ***,unlike DNNs,shallow convolutional neural networks often outperform deeper models in mitigating overfitting,particularly with small ***,many of these methods rely on a single feature for recognition,resulting in an insufficient ability to extract highly effective *** address this limitation,in this paper,an Improved Dual-stream Shallow Convolutional Neural Network based on an Extreme Gradient Boosting Algorithm(IDSSCNN-XgBoost)is introduced for ME *** proposed method utilizes a dual-stream architecture where motion vectors(temporal features)are extracted using Optical Flow TV-L1 and amplify subtle changes(spatial features)via EulerianVideoMagnification(EVM).These features are processed by IDSSCNN,with an attention mechanism applied to refine the extracted effective *** outputs are then fused,concatenated,and classified using the XgBoost *** comprehensive approach significantly improves recognition accuracy by leveraging the strengths of both temporal and spatial information,supported by the robust classification power of *** proposed method is evaluated on three publicly available ME databases named Chinese Academy of Sciences Micro-expression Database(CASMEII),Spontaneous Micro-Expression Database(SMICHS),and Spontaneous Actions and Micro-Movements(SAMM).Experimental results indicate that the proposed model can achieve outstanding results compared to recent *** accuracy results are 79.01%,69.22%,and 68.99%on CASMEII,SMIC-HS,and SAMM,and the F1-score are 75.47%,68.91%,and 63.84%,*** proposed method has the advantage of operational efficiency and less computational time.

关键词： ME recognition dual stream shallow convolutional neural network euler video magnification TV-L1 XgBoost

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Establishing a new testing protocol of the electrochemical stability window for electric double-layer capacitors

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Science China Chemistry 2025年第1期68卷 217-225页

作者： Ruilin Hou Xiaoxi zhao Tingting Liang Qingyun Dou Shan Xu Xingbin yan School of Materials Science and Engineering Sun Yat-sen University Laboratory of Clean Energy Chemistry and Materials Lanzhou Institute of Chemical Physics Chinese Academy of Sciences College of Engineering and Applied Sciences Nanjing University Center of Industrial Analysis and Testing Guangdong Academy of Science

The electrochemical stability window(ESW) is crucial for determining the energy density of electric double-layer capacitors(EDLCs). However, current ESW testing protocols of EDLCs are strongly affected by artificial factors, thereby leading to unreliable result. To solve this problem, we first identify the key shortcomings of the traditional protocols by analyzing their test principles. Subsequently, taking an aqueous carbon-based EDLC as an example, we design a side reaction descriptor to represent the response difference in the charging capacitance to side reactions between the cyclic voltammetry and galvanostatic chargedischarge methods. With the aid of this descriptor, we propose a new ESW testing protocol, which is not only able to completely avoid the adverse influence of subjective factors but also fully consider the operating condition limitations. The protocol can be extended from the single electrode to the full device. This work proposes a more rigorous definition of ESW and provides a reliable protocol for evaluating the ESW of EDLCs.

关键词： electric double-layer capacitors electrochemical stability window testing protocol side reaction descriptor operating limitation

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对日美贸易摩擦的回顾与展望

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现代日本经济 2001年第4期118卷 1-5,44页

作者：赵春明何艳北京师范大学经济学院北京 100875

日美贸易摩擦是战后日美经济贸易关系中一个非常突出的问题，是日美全部经济摩擦的基点，在相当程度上决定着两国其它方面摩擦的产生、发展和解决方式。本文将在简要回顾日美贸易摩擦的历史发展进程的基础上，分析其特点及其产生根源，... 详细信息

日美贸易摩擦是战后日美经济贸易关系中一个非常突出的问题，是日美全部经济摩擦的基点，在相当程度上决定着两国其它方面摩擦的产生、发展和解决方式。本文将在简要回顾日美贸易摩擦的历史发展进程的基础上，分析其特点及其产生根源，并进一步探讨新世纪日美贸易摩擦的发展趋势。

关键词：日美贸易摩擦发展趋势日本美国

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Channel pruning on frequency response

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Science China(Information Sciences) 2025年第1期68卷 159-170页

作者： Hang LIN Yifan PENG Lin BIE Chenggang yan Xibin zhao Yue GAO School of Software Tsinghua University School of Automation Hangzhou Dianzi University

Network pruning has a significant role in reducing network parameters and accelerating the inference time of the network. Some existing methods prune the network based on the frequency of the data, and finally obtain a sub-network with high accuracy. However, according to our experimental analysis, different frequencies of information in the data contribute differently to the accuracy of the model, and using this information directly for pruning without making a selection will lead to incorrect results. We believe that pruning should retain the convolutional kernels in the network that process important information, while those kernels that process unimportant information should be removed. In this paper, we first investigate the meaning of each frequency band information in the spectrum and their contribution to the prediction accuracy of the network,and according to these results, we propose a new pruning method based on frequency response(PFR). Our PFR finds and removes the convolutional kernels in the network that specialize in processing unimportant information, resulting in a compact neural network model. PFR obtains significant experimental results on different datasets, for example, a 56.0% raduction of float points operations(FLOPs) on Res Net-50 and only 0.37% of Top-1 accuracy degradation on the Image Net dataset.

关键词： deep learning model compression filter pruning channel pruning frequency response

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