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Clinical study of customized aspherical intraocular lens implants

International Journal of Ophthalmology(English edition) 2014年第5期7卷 816-821页

作者： lie-Xi Jia zhao-hui li Department of Ophthalmology Chinese PLA General Hospital

AIM:To compare if there is an improvement in visual functions with age-related cataracts between patients receiving a aspherical intraocular lens(IOL) based on corneal wavefront aberration and patients randomly assigned ***:A total of 124 eyes of 124 patients with age-related cataracts were placed in experimental group and a group receiving randomly assigned(RA) *** experimental group was undergone Pentacam corneal spherical aberration measurement before surgery; the targeted range for residual total spherical aberration after surgery was set to 0-0.3 μm. Patients with a corneal spherical aberration <0.3 μm were implanted with a zero-spherical aberration advanced optics(AO) aspherical IOL and patients with an aberration ≥0.3 μm received a Tecnis Z9003 aspherical lens in experimental group. RA patients were randomly implanted with an AO lens or a Tecnis Z9003 lens. Three months after surgery total spherical aberration, photopic/mesopic contrast sensitivities, photopic/mesopic with glare contrast sensitivities, and logMAR vision were ***:Statistical analysis on logMAR vision showed no significant difference between two groups(P =0.413). The post-surgical total spherical aberration was 0.126 ±0.097 μm and 0.152 ±0.151 μm in the experimental and RA groups, respectively(P =0.12). The mesopic contrast sensitivities at spatial frequencies of 6,12 and 18 c/d in the experimental group were significantly higher than of the RA group(P =0.00; P =0.04;P =0.02). The mesopic with glare contrast sensitivity in the experimental group at a spatial frequency of 18 c/d was also significantly higher vs the RA group(P =0.01).CONCLUSION:Pre-surgical corneal spherical aberration measurement in cataract patients followed bycustomized selection of aspherical IOL implants improved mesopic contrast sensitivities at high spatial frequencies, and thus is a superior strategy compared to the random selection of aspherical intraocular lens implants.

关键词： intraocular lens cataract extraction corneal wavefront aberration mesopic vision night vision

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Effect of Aβ protein on inhibiting proliferation and promoting apoptosis of retinal pigment epithelial cells

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International Journal of Ophthalmology(English edition) 2018年第6期11卷 929-934页

作者： Zi Ye Shou-Zhi He zhao-hui li Department of Ophthalmology the Chinese PLA General Hospital

AIM： To identify the effect and regulatory mechanism of amyloid β （Aβ） protein on retinal pigment epithelial （RPE） cells in cell proliferation and apoptosis, and clarify Aβ role in the pathogenesis of age-related macular degeneration （AMD）. METHODS： The model of Aβ25-35 protein cytotoxicity in RPE cell was successfully established to investigate the effect of Aβ protein on RPE cells in vitro. Based on Aβ protein, the specific inhibitors （HY-50682 or BAY11-7082） or activating agent （lipopolysaccharide） was used to analyze the regulatory mechanism of Aβ protein to RPE cells on cell proliferation and apoptosis by flow cytometry, real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay and dual-luciferase reporter gene assay. RESULTS： The number of RPE cells, treated with Aβ25-35 from 0.3 to 60 μmol/L, significantly reduce （P〈0.01）, and had the dose-dependent effect. Aβ protein 60 μmol/L inhibits the G1/S phase transition （P〈0.01） and down-regulated cyclin E mRNA level （P〈0.01）. Similarly, Aβ25-35 induced a significant increase of cell apoptosis, accompanied by the significantly higher level of activated caspase 3 protein. Furthermore, nuclear factor-kappaB （NF-κB） activity and hosphorylated Iκ-Ba level would significantly lower in treated RPE cells. Using specific inhibitors or activating agent based on the Aβ, the cell numbers, NF-κB activity, phosphorylated Iκ-Ba level, receptor for advanced glycation endproducts （RAGE） gene expression levels, cyclin E mRNA level and activated caspase 3 level had accordingly changed by different methods, confirming that RAGE/NF-κB signaling pathway involved in the regulation of Aβ protein on RPE cell apoptosis and proliferation. CONCLUSION： Aβ protein inhibits cell proliferation and activates apoptosis via inactivation of the RAGE/NF-κB signaling pathway in RPE cell.

关键词： amyloid β protein retinal pigment epithelialcells proliferation apoptosis receptor for advanced glycation endproducts nuclear factor-kappaB age-related macular degeneration

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Phenotype of Usher syndrome type Ⅱ assosiated with compound missense mutations of c.721 C>T and c.1969 C>T in MYO7A in a Chinese Usher syndrome family

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International Journal of Ophthalmology(English edition) 2015年第4期8卷 670-674页

作者： Wei Zhai Xin Jin Yan Gong ling-hui Qu Chen zhao zhao-hui li Department of Ophthalmology General Hospital of Chinese PLA School of Medicine Nan Kai University Key Lab of Visual Damage and Regeneration & Restoration of Chongqing Department of Ophthalmology No.181 Hospital of Guilin

·AIM: To identify the pathogenic mutations in a Chinese pedigree affected with Usher syndrome type II(USH2).· METHODS: The ophthalmic examinations and audiometric tests were performed to ascertain the phenotype of the family. To detect the genetic defect,exons of 103 known RDs-associated genes including 12 Usher syndrome(USH) genes of the proband were captured and sequencing analysis was performed to exclude known genetic defects and find potential pathogenic mutations. Subsequently, candidate mutations were validated in his pedigree and 100 normal controls using polymerase chain reaction(PCR) and Sanger sequencing.·RESULTS: The patient in the family occurred hearing loss(HL) and retinitis pigmentosa(RP) without vestibular dysfunction, which were consistent with standards of classification for USH2. He carried the compound heterozygous mutations, c.721 C >T and c.1969 C >T, in the MYO7 A gene and the unaffected members carried only one of the two mutations. The mutations were not present in the 100 normal controls.· CONCLUSION: We suggested that the compound heterozygous mutations of the MYO7 A could lead to USH2, which had revealed distinguished clinical phenotypes associated with MYO7 A and expanded the spectrum of clinical phenotypes of the MYO7 A mutations.

关键词： Usher syndrome mutation MYO7A next-generation sequencing

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Generation of Leydig-like cells:approaches,characterization,and challenges

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Asian Journal of Andrology 2022年第4期24卷 335-344页

作者： zhao-hui li Jun-Dong Lu Shi-Jun li Hao-lin Chen Zhi-Jian Su Guangdong Provincial Key Laboratory of Bioengineering Medicine Department of Cell BiologyJinan UniversityGuangzhou 510632China lnstitute of life Sciences Wenzhou UniversityWenzhou 325035China Department of Gynecology and Obstetrics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhou 325027China National Engineering Research Center of Genetic Medicine Jinan UniversityGuangzhou 510632China

Testosterone production by Leydig cells(LCs)plays a crucial role in male *** functional degeneration of LCs can cause testosterone deficiency,ultimately resulting in primary male *** of exogenous LCs with the ability to produce testosterone in response to the regulation of the hypothalamus-pituitary-gonad axis could be a promising alternative option to treat male primary *** studies have shown that it is possible to generate Leydig-like cells from stem cells by various *** addition,somatic cells,such as embryonic or adult fibroblasts,have also been successfully reprogrammed into Leydig-like *** this review,we summarized the recent advances in the generation of Leydig-like cells,with an emphasis on comparing the effectiveness and safety of different protocols used and the cells *** further analyzing the characteristics of Leydig-like cells generated from fibroblasts based on small signaling molecules and regulatory factors,we found that although the cells may produce testosterone,they are significantly different from real *** future in vivo applications,it is important that the steroidogenic cells generated be evaluated not only for their steroidogenic functions but also for their overall cell metabolic state by proteomics or transcriptomic tools.

关键词： fibroblasts hypogonadism Leydig-like cells reprogramming stem cells

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Graphene oxide wrapped magnetic nanoparticle composites induced by SiO_(2) coating with excellent regenerability

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International Journal of Minerals,Metallurgy and Materials 2021年第12期28卷 2001-2007页

作者： Zhong-liang Hu Hou-quan Cui Yan-huai Ding Jing-ying li Yi-rong Zhu zhao-hui li College of Metallurgy and Material Engineering Hunan University of TechnologyZhuzhou 412007China College of Chemistry Xiangtan UniversityXiangtan 411105China Institute of Fundamental Mechanics and Materials Engineering Xiangtan UniversityXiangtan 411105China

Graphene oxide(GO)wrapped Fe_(3)O_(4) nanoparticles(NPs)were prepared by coating the Fe_(3)O_(4) NPs with a SiO_(2) layer,and then modifying by amino groups,which interact with the GO nanosheets to form covalent *** SiO_(2) coating layer plays a key role in integrating the magnetic nanoparticles with the GO *** effect of the amount of SiO_(2) on the morphology,structure,adsorption,and regenerability of the composites was studied in *** appropriate SiO_(2) layer can effectively induce the GO nanosheets to completely wrap the Fe_(3)O_(4) NPs,forming a core-shell Fe_(3)O_(4)@SiO_(2)@GO composite where Fe_(3)O_(4)@SiO_(2) NPs are firmly encapsulated by GO *** optimized Fe_(3)O_(4)@SiO_(2)@GO sample exhibits a high saturated adsorption capacity of 253 mg·g^(-1) Pb(Ⅱ)cations from wastewater,and the adsorption process is well fitted by Langmuir adsorption ***,the composite displays excellent regeneration,maintaining a~90%adsorption capacity for five cycles,while other samples decrease their adsorption capacity *** work provides a theoretical guidance to improve the regeneration of the GO-based adsorbents.

关键词： composites composite coating

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Expression and Characterization of HIV-1 Envelope Glycoprotein in Pichia Pastoris

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Chemical Research in Chinese Universities 2008年第3期24卷 316-321页

作者： zhao li-hui YU Xiang-hui JIANG Chun-lai WU Yong-ge SHEN Jia-cong KONG Wei Changchun University of Science and Techinology Changchun 130022 P. R. China College of life Science for Supramolecular Structure and Materials Jilin University Changchun 130012 P. R. China State Key Laboratory for Supramoleeular Structure and Materials Jilin University Changehun 130012 P. R. China

To obtain a sufficient amount of glycoprotein for further studying the structure and function of HIV-1 envelope glycoprotein, amplified and modified HIV-1 envelope glycoprotein gene which recombined subtypes（850 amino acids） from Guangxi in China was inserted into Pichia pastoris expression vector pPICZαB; then the recombinant plasmid was transported into the yeast cells to induce the expression of Env protein with methanol. The results of SDS-PAGE and Western blot indicate that the envelope glycoprotein could be expressed in Pichia pastoris with productions of a 120000 glycoprotein and a 41000 glycoprotein, which showed satisfactory immunogenicity by indirect EliSA.

关键词： Pichia pastoris env gene Glycoprotein Epitope Immunogenicity

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Theoretical Design of Defects as a Driving Force for Ion Transport in li_(3)OBr Solid Electrolyte

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Energy & Environmental Materials 2024年第3期7卷 143-153页

作者： Xingyun Luo Yanlu li Xian zhao State Key Lab of Crystal Materials and Institute of Crystal Materials Shandong UniversityJinan 250100China Center for Optics Research and Engineering Shandong UniversityQingdao 266237China

Due to ever-increasing concerns about safety issues in using li ionic batteries,solid electrolytes have extensively *** li-rich antiperovskite li_(3)OBr has been considered as a promising solid electrolyte candidate,but it still suffers challenges to achieve a high ionic conductivity owing to the high intrinsic symmetry of the crystal ***,we presented a design strategy that introduces various point defects and grain boundaries to break the high lattice symmetry of li_(3)OBr crystal,and their effect and microscopic mechanism of promoting the migration of li-ion were explored *** has been found that li_(i)are the dominant defects responsible for the fast li-ion diffusion in bulk li_(3)OBr and its surface,but they are easily trapped by the grain boundaries,leading to the annihilating of the Frenkel defect pair V'_(li)+li_(i),and thus limits the V'_(li)diffusion at the grain *** V_(Br)defect near the grain boundaries can effectively drive V'_(li)across the grain boundary,thereby converting the carrier of li^(+)migration from li,in the bulk and surface to V'_(li)at the grain boundary,and thus improving the ionic conductivity in the whole li_(3)OBr *** work provides a comprehensive insight into the li^(+)transport and conduction mechanism in the li_(3)OBr *** opens a new way of improving the conductivity for all-solid-state li electrolyte material through the defect design.

关键词： defects density functional theory ionic migration solid electrolyte

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Various phenotypes of autosomal dominant cone-rod dystrophy with cone-rod homeobox mutation in two Chinese families

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International Journal of Ophthalmology(English edition) 2022年第12期15卷 1915-1923页

作者： hui Cui Xin Jin Qing-Hua Yang ling-hui Qu Bao-Ke Hou zhao-hui li Hou-Bin Huang Senior Department of Ophthalmology the Third Medical Center of PLA General HospitalBeijing 100853China Department of Ophthalmology the 74th Army Group HospitalGuangzhou 510318Guangdong ProvinceChina Department of Ophthalmology Hainan Hospital of Chinese PLA General HospitalSanya 572000Hainan ProvinceChina

AIM:To present the clinical manifestations of 5 autosomal dominant cone-rod dystrophy(ad CORD)patients from two Chinese families with cone-rod homeobox(CRX)mutation(p.R41W),and to explore the clinical heterogeneity of ad CORD with CRX mutation(p.R41W).METHODS:Interrogation and ophthalmological examinations were undertaken in all patients and unaffected *** of clinical features was performed by visual acuity,slit lamp examination,visual field examination,fundoscopy,autofluorescence and spectral domain optical coherence *** next-generation sequencing was applied as a useful tool to identify the causative mutation of CORD ***:A CRX missense mutation c.121C>T was identified in all patients,resulting in an amino acid change from arginine acid to tryptophan(p.R41W).The patients presented with early onset,progressive and different severities with ***:This is the first report of the clinical phenotype of CRX mutation(p.R41W)in Chinese families,and the mutation can lead to a wide range of various retinal phenotypes.

关键词： cone-rod homeobox cone-rod dystrophy mutation

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Length-Heterogeneity Polymerase Chain Reaction as a Diagnostic Tool for Bacterial Vaginosis

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Chinese Medical Journal 2018年第18期131卷 2242-2243页

作者： Xiao-Xi Niu Ren-Hua Sun zhao-hui li Yao-hui Bai Bing-Bing Xiao Department of Obstetrics and Gynecology Peking University First Hospital Beijing 100034 China Rural Energy and Environment Agency Ministry of Agriculture Beijing China Key Laboratory of Drinking Water Science and Technology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China

To the Editor： Bacterial vaginosis （BV） is the most prevalent form ofvaginitis among women of reproductive age, affecting 8-23% of women globally. Clinically, BV is typically diagnosed using Amsel＇s criteria and the Nugent scoring system. However, these methods are inaccurate in many cases. In this study, we explored the feasibility of using length-heterogeneity-polymerase chain reaction （LH-PCR） for diagnosis of BV.

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Comparison of nonlinear modeling methods for the composite rubber clamp

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Applied Mathematics and Mechanics(English Edition) 2024年第5期45卷 763-778页

作者： Yiming CAO hui MA Xumin GUO Bingfeng zhao hui li Xin WANG Bing WANG School of Mechanical Engineering and Automation Northeastern UniversityShenyang 110819China Key Laboratory of Vibration and Control of Aero-Propulsion Systems of Ministry of Education of China Northeastern UniversityShenyang 110819China AVIC Shenyang Engine Design Institute Shenyang 110013China Dalian Mengyu Machinery Co. Ltd.Dalian 116317Liaoning ProvinceChina

The cubic stiffness force model(CSFM)and Bouc-Wen model(BWM)are introduced and compared *** unknown coefficients of the nonlinear models are identified by the genetic algorithm combined with *** fitting the identified nonlinear coefficients under different excitation amplitudes,the nonlinear vibration responses of the system are *** results show that the accuracy of the BWM is higher than that of the CSFM,especially in the non-resonant ***,the optimization time of the BWM is longer than that of the CSFM.

关键词： pipeline system nonlinear clamp model composite rubber clamp amplitude-dependent characteristic vibration response experiment

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