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Synthetic and Systems Biotechnology 2024年第1期9卷 152-158页

作者： Xiaoning Xu Lingwen Zhang Yue Cui jian Kong Tingting guo State Key Laboratory of Microbial Technology Shandong UniversityNo.72 Binhai RoadQingdao266237PR China

Lactococcus lactis and Streptococcus thermophilus are considered as ideal chassis of engineered probiotics,while food-grade genetic tools are limited in those ***,a Zn^(2+)-controlled gene expression(ZICE)system was identified in the genome of *** CGMCC7.179,including a transcriptional regulator sczAst and a promoter region of cation transporter czcD(PczcDst).Specific binding of the SczAst to the palindromic sequences in PczcDst was demonstrated by EMSA analysis,suggesting the regulation role of SczAst on *** evaluate their possibility to control gene expression in vivo,the sczAst-PczcDst was employed to drive the expression of green fluorescence protein(GFP)gene in *** NZ9000 and *** CGMCC7.179,*** of the transformants could express GFP under Zn^(2+)induction,while no fluorescence without Zn^(2+)*** optimal conditions,Zn^(2+)was used at a final concentration of 0.8 mM in *** and 0.16 mM in *** at OD600 close to 0.4,and omitting yeast extract powder in the medium unexpectedly improved GFP expression level by *** the help of the ZICE system,engineered *** and *** strains were constructed to secret cytokine interleukin-10(IL-10)with immunogenicity,and the IL-10 content in the supernatant of the engineered *** was 59.37%of that under the nisin controlled expression *** study provided a tightly controlled expression system by the food-grade inducer Zn^(2+),having potential in development of engineered probiotics.

关键词： Lactococcus lactis Streptococcus thermophilus Food-grade expression system Engineered probiotic

Hexagonal boron nitride based slippery liquid infused porous surface with anti-corrosion, anti-contaminant and anti-icing properties for protecting magnesium alloy

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Chinese Chemical Letters 2024年第4期35卷 532-538页

作者： Wenhao Yan Shuaiya Xue Xuerui zhao Wei Zhang jian Li Key Laboratory of Eco-Functional Polymer Materials of the Ministry of Education College of Chemistry and Chemical EngineeringNorthwest Normal UniversityLanzhou 730070China

In an era where the concept of green development is deeply rooted, magnesium(Mg) alloy as a light metal has a long-term development prospect in the process of energy saving, emission reduction and environmental improvement. However, anti-corrosion performance of Mg alloy is poor due to the high chemical activity and low equilibrium potential, which limits the development of Mg alloy ***, three-dimensional mesopore hollow polypyrrole spheres(MHPS) were prepared, and the MHPS was inserted into the middle of the stacked hexagon boron nitride(h-BN) lamellae, which allowed the hBN to be separated forming a further composite with abundant pore structure. Subsequently, the MHPS/hBN-OH composite was uniformly sprayed on the Mg alloy surface via simple spraying method to form the superhydrophobic surface(SHS). Finally, the slippery liquid infused porous surface(SLIPS) was successfully fabricated by applying drops of silicone lubricant on the superhydrophobic coating surface. After a series of characterization and testing, the results showed that the stacking of h-BN lamellae was significantly reduced after h-BN was successfully embedded by MHPS. In addition, the fabricated SLIPS have excellent self-cleaning, mechanical stability, anti-icing and anti-corrosion properties. Therefore, the method of embedding polymer microspheres not only offers a new strategy for h-BN exfoliation, but also the successful prepared SLIPS largely retards the corrosion of Mg alloy while providing new ideas for the development of SLIPS.

关键词： Anti-corrosion Mg alloy Hexagon boron nitride Polypyrrole SLIPS

Genetic and molecular mechanisms of reproductive isolation in the utilization of heterosis for breeding hybrid rice

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Journal of Genetics and Genomics 2024年第6期51卷 583-593页

作者： Chaolong Wang Xiaowen Yu jian Wang Zhigang zhao jianmin Wan State Key Laboratory for Crop Genetics&Germplasm Enhancement and Utlization Nanjing Agricultural UniversityZhongshan Biological Breeding LaboratoryNanjingJiangsu210095China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Heterosis,also known as hybrid vigor,is commonly observed in rice *** hybridization of rice species or subspecies exhibits robust hybrid vigor,however,the direct harnessing of this vigor is hindered by reproductive ***,we review recent advances in the understanding of the molecular mechanisms governing reproductive isolation in inter-subspecific and inter-specific *** review encompasses the genetic model of reproductive isolation within and among Oryza sativa species,emphasizing the essential role of mitochondria in this ***,we delve into the molecular intricacies governing the interaction between mitochondria and autophagosomes,elucidating their significant contribution to reproductive ***,our exploration extends to comprehending the evolutionary dynamics of reproductive isolation and speciation in *** on these advances,we offer a forward-looking perspective on how to overcome the challenges of reproductive isolation and facilitate the utilization of heterosis in future hybrid rice breeding endeavors.

关键词： Hybrid vigor Reproductive isolation Hybrid ricebreeding

Efficiency-oriented vehicle relocation of shared autonomous electric fleet in station-based car-sharing system

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Journal of Traffic and Transportation Engineering(English Edition) 2024年第4期11卷 681-699页

作者： Ning Wang Hangqi Tian Jiahui guo Huahua Wu jian Lyu School of Automotive Studies Tongji UniversityShanghai 201804China State Grid Zhejiang Electric Power Co. Ltd.Hangzhou 310007China State Grid Zhejiang Electric Vehicle Service Co. Ltd.Hangzhou 310012China

Long waiting delays for users and significant imbalances in vehicle distribution are bothering traditional station-based one-way electric car-sharing system *** address the problems above,a“demand forecast-station status judgement-vehicle relocation”multistage dynamic relocation algorithm based on the automatic formation cruising technology was proposed in this *** stage one,a novel trip demand forecast model based on the long short-term memory network was established to predict users'car-pickup and car-return order volumes at each *** stage two,a dynamic threshold interval was determined by combining the forecast results with the actual vehicle distribution among stations to evaluate the status of each *** vehicle-surplus,vehicleinsufficient,vehicle-normal stations,and the number of surplus or insufficient vehicles for each station were *** stage three,setting driving mileage and carbon emission as the optimization objectives,an integer linear programming mathematical model was constructed and the optimal vehicle relocation scheme was obtained by the commercial solver *** 43 stations and 187 vehicles in Jiading District,Shanghai,China,as a case study,results showed that rapid vehicle rebalancing among stations with minimum carbon emissions could be realized within 15 min and the users’car-pickup and car-return demands could be fully satisfied without any refusal.

关键词： Sustainable transportation One-way car-sharing Shared autonomous electric fleet Multistage dynamic relocation algorithm

Numerical simulation analysis of wind field above pavement surface in different landforms by computational fluid dynamics approach

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Journal of Traffic and Transportation Engineering(English Edition) 2024年第3期11卷 559-573页

作者： jian Ouyang zhao jiang Leiyu Ye Jing Li Peng Cao School of Civil Engineering and Architecture Hainan UniversityHaikou 570000China School of Transportation and Logistics Dalian University of TechnologyDalian 116024China Hexagon Manufacturing Intelligence(Qingdao)Co. Ltd.Qingdao 266000China China Tower Co. Ltd.Dalian BranchDalian 116024China Faculty of Architecture Civil and Transportation EngineeringBeijing University of TechnologyBeijing 100124China

The temperature and moisture of the pavement structure can be greatly influenced by the wind speed above pavement *** wind speed above pavement surface not only is dominated by the wind speed of atmosphere,but also it is highly related to the landform and buildings around ***,currently there are no studies about the wind field above pavement surface in consideration of the effect of the landform and buildings.A simulation method,which is combined with geographic information system(GIS),wind data from meteorological observatory and computational fluid dynamics(CFD)software,is employed to study the effect of the landform and the wind speed of atmosphere on the wind field above pavement *** cases are studied,including an urban road,a coastal road and a mountainous ***,the wind field distribution above road surface in different wind directions was studied in our *** indicate that the wind field above pavement surface can be greatly affected by the landforms,buildings and wind *** simulation method can provide reliable results for the wind field above pavement *** maximum relative errors between simulated and measured wind speed can be less than 20%in the analysis of the three *** is recommended that the CFD simulation method is a good tool to accurately know the wind field above pavement surface.

关键词： Wind field above pavement surface Computational fluid dynamics Landforms Geographic information system Simulation

Recent progress on Sn_(3)O_(4)nanomaterials for photocatalytic applications

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International Journal of Minerals,Metallurgy and Materials 2024年第2期31卷 231-244页

作者： Xin Yu Congcong Li jian Zhang Lili zhao Jinbo Pang Longhua Ding Institute for Advanced Interdisciplinary Research(iAIR) School of Chemistry and Chemical EngineeringUniversity of JinanJinan 250022China School of Sino-German Engineering Shanghai Technical Institute of Electronics and InformationShanghai 201411China Division of Systems and Synthetic Biology Department of Life SciencesChalmers University of Technology41296 GöteborgSweden

Tin(IV)oxide(Sn_(3)O_(4))is layered tin and exhibits mixed valence *** has emerged as a highly promising visible-light pho-tocatalyst,attracting considerable *** comprehensive review is aimed at providing a detailed overview of the latest advance-ments in research,applications,advantages,and challenges associated with Sn_(3)O_(4)photocatalytic *** fundamental con-cepts and principles of Sn_(3)O_(4)are ***_(3)O_(4)possesses a unique crystal structure and optoelectronic properties that allow it to ab-sorb visible light efficiently and generate photoexcited charge carriers that drive photocatalytic ***,strategies for the control and improved performance of Sn_(3)O_(4)photocatalytic nanomaterials are *** control,ion doping,and hetero-structure construction are widely employed in the optimization of the photocatalytic performance of Sn_(3)O_(4)*** effective imple-mentation of these strategies improves the photocatalytic activity and stability of Sn_(3)O_(4)***,the review explores the diverse applications of Sn_(3)O_(4)photocatalytic nanomaterials in various fields,such as photocatalytic degradation,photocatalytic hydro-gen production,photocatalytic reduction of carbon dioxide,solar cells,photocatalytic sterilization,and optoelectronic *** discus-sion focuses on the potential of Sn_(3)O_(4)-based nanomaterials in these applications,highlighting their unique attributes and ***,the review provides an outlook on the future development directions in the field and offers guidance for the exploration and de-velopment of novel and efficient Sn_(3)O_(4)-based *** the identification of emerging research areas and potential avenues for improvement,this review aims to stimulate further advancements in Sn_(3)O_(4)-based photocatalysis and facilitate the translation of this promising technology into practical applications.

关键词： photocatalysis Sn_(3)O_(4)nanomaterials building heterostructures antibacterial therapy water splitting

Accelerating Factor Xa inhibitor discovery with a de novo drug design pipeline

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Chinese Journal of Chemical Engineering 2024年第8期72卷 85-94页

作者： Yujing zhao Qilei Liu jian Du Qingwei Meng Liang Sun Lei Zhang State Key Laboratory of Fine Chemicals Frontiers Science Center for Smart Materials Oriented Chemical EngineeringInstitute of Chemical Process Systems EngineeringSchool of Chemical EngineeringDalian University of TechnologyDalian 116024China Ningbo Institute of Dalian University of Technology Ningbo 315016China Shenzhen Shuli Tech Co. LtdShenzhen 518126China Department of Physics City University of Hong KongTat Chee AvenueKowloonHong Kong SARChina

Small-molecule drugs are essential for maintaining human health. The objective of this study is to identify a molecule that can inhibit the Factor Xa protein and be easily procured. An optimization-based de novo drug design framework, Drug CAMD, that integrates a deep learning model with a mixed-integer nonlinear programming model is used for designing drug candidates. Within this framework, a virtual chemical library is specifically tailored to inhibit Factor Xa. To further filter and narrow down the lead compounds from the designed compounds, comprehensive approaches involving molecular docking,binding pose metadynamics(BPMD), binding free energy calculations, and enzyme activity inhibition analysis are utilized. To maximize efficiency in terms of time and resources, molecules for in vitro activity testing are initially selected from commercially available portions of customized virtual chemical libraries. In vitro studies assessing inhibitor activities have confirmed that the compound EN300-331859shows potential Factor Xa inhibition, with an IC_(50)value of 34.57 μmol·L^(-1). Through in silico molecular docking and BPMD, the most plausible binding pose for the EN300-331859-Factor Xa complex are identified. The estimated binding free energy values correlate well with the results obtained from biological assays. Consequently, EN300-331859 is identified as a novel and effective sub-micromolar inhibitor of Factor Xa.

关键词： Chemical product design Mathematical programming method Deep learning Binding affinity Factor Xa inhibitor

Silkworm pupa peptide ameliorates dextrose sodium sulfate-induced colitis by enhancing gut barriers and modifying gut flora

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Food Science and Human Wellness 2024年

作者： Yaxi Zhou Diandian Wang Hao Duan Shiqi Zhou Jinhong guo Yu guo jian zhao Wenjie Yan Beijing Key Laboratory of Bioactive Substances and Functional Food， College of Biochemical Engineering， Beijing Union University

The anti-inflammatory properties of silkworm pupa peptide (SPP) have been recognized for their potential benefits in colitis treatment. However， the underlying mechanism of SPP in colitis animal models remains unclear. The objective of our work was to investigate the improving effect and the mechanism of action of SPP on a mouse model of dextrose sodium sulfate (DSS)-induced colitis. The findings suggested that SPP effectively eased ulcerative colitis (UC) symptoms in mice by decreasing disease activity index， ameliorating histopathological injury， and decreasing serum and colonic markers of inflammation. In addition， our research findings demonstrated that SPP restored intestinal barrier function by increasing the production of tight junction proteins such as zonula occludens-1 (ZO-1)， claudin-1， claudin-3， occludin， and adherens junction protein E-cadherin1. Further， SPP supplementation enhanced the concentration of short-chain fatty acids and positively altered the makeup of the gut bacteria in the mice’s gut. These findings underscore SPP’s ability to slow the progression of colitis and point to its possible use as a functional component in dietary supplements for the prevention of early-stage colitis.

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Visualization and characterization of experimental hydraulic fractures interacting with karst fracture-cavity distributions

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1667-1683页

作者： Hanzhi Yang Xin Chang Chunhe Yang Wuhao guo Lei Wang guokai zhao Yintong guo State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing400044China State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China

Karst fracture-cavity carbonate reservoirs,in which natural cavities are connected by natural fractures to form cavity clusters in many circumstances,have become significant fields of oil and gas exploration and *** fracturing is considered as the best method for exploiting carbonate reservoirs;however,previous studies primarily focused on the effects of individual types of geological formations,such as natural fractures or cavities,on fracture *** this study,true-triaxial physical simulation experiments were systematically performed under four types of stress difference conditions after the accurate prefabrication of four types of different fracture-cavity distributions in artificial ***,the interaction mechanism between the hydraulic fractures and fracture-cavity structures was systematically analyzed in combination with the stress distribution,cross-sectional morphology of the main propagation path,and three-dimensional visualization of the overall fracture *** was found that the propagation of hydraulic fractures near the cavity was inhibited by the stress concentration surrounding the *** contrast,a natural fracture with a smaller approach angle(0°and 30°)around the cavity can alleviate the stress concentration and significantly facilitate the connection with the *** addition,the hydraulic fracture crossed the natural fracture at the 45°approach angle and bypassed the cavity under higher stress difference conditions.A new stimulation effectiveness evaluation index was established based on the stimulated reservoir area(SRA),tortuosity of the hydraulic fractures(T),and connectivity index(CI)of the *** findings provide new insights into the fracturing design of carbonate reservoirs.

关键词： Karst fracture-cavity reservoir Fracturing experiment Fracture propagation Cross-sectional morphology Stimulation effectiveness

Attaining synergetic equilibrium of electrical conductivity and tensile strength in GQDs@GN/Cu composites through multi-scale intragranular and intergranular reinforcements

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Rare Metals 2024年第1期43卷 366-379页

作者： Shuang-Yin Zhang Liang Liu Rui Bao jian-Hong Yi Sheng-Da guo Faculty of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China Key Laboratory of Advanced Materials of Yunnan Province Kunming University of Science and TechnologyKunming 650093China Faculty of Materials Metallurgy and Chemistry Jiangxi University of Science and TechnologyGanzhou 341000China

The configuration and quality of reinforcements, as well as the robustness of interfacial bonding,holding a critical significance in determining the concurrence between electrical conductivity and mechanical strength in metal matrix composites. In this study, citric acid was employed as the precursor for synthesizing multiscale carbon nanomaterials(graphene quantum dots and graphene, abbreviated as GQDs and GN). The GQDs@GN/Cu composites were fabricated through a segmented ball milling process in conjunction with subsequent spark plasma sintering(SPS). The intragranular GQDs and intergranular GQDs@GN had synergistically reinforced Cu composites through Orowan strengthening, load transfer strengthening and refinement strengthening. Furthermore,the robust interface bonding between GQDs@GN and Cu effectively mitigated interfacial impedance stemming from electron-boundary scattering. The yield strength and ultimate tensile strength of the GQDs@GN/Cu composites were recorded as 270 and 314 MPa, respectively, representing an improvement of 92 and 28% over pure Cu, while maintaining electrical conductivity at a level comparable to that of pure Cu. This study advances the understanding of the possibility of realizing a synergistic compatibility between electrical conductivity and mechanical strength in Cu composites.

关键词： Cu matrix composites Interface bonding Mechanical property Graphene quantum dots(GQDs) Electrical conductivity